ID   TEF_HUMAN               Reviewed;         303 AA.
AC   Q10587; B0QYS8; B2RC22; Q15729; Q7Z3J7; Q8IU94; Q96TG4;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 3.
DT   24-JAN-2024, entry version 193.
DE   RecName: Full=Thyrotroph embryonic factor;
GN   Name=TEF; Synonyms=KIAA1655;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=7835883; DOI=10.1006/geno.1994.1510;
RA   Khatib Z.A., Inaba T., Valentine M., Look A.T.;
RT   "Chromosomal localization and cDNA cloning of the human DBP and TEF
RT   genes.";
RL   Genomics 23:344-351(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=8639829;
RA   Hunger S.P., Li S., Fall M.Z., Naumovski L., Cleary M.L.;
RT   "The proto-oncogene HLF and the related basic leucine zipper protein TEF
RT   display highly similar DNA-binding and transcriptional regulatory
RT   properties.";
RL   Blood 87:4607-4617(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=15461802; DOI=10.1186/gb-2004-5-10-r84;
RA   Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A.,
RA   Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J.,
RA   Beare D.M., Dunham I.;
RT   "A genome annotation-driven approach to cloning the human ORFeome.";
RL   Genome Biol. 5:R84.1-R84.11(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P.,
RA   Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y.,
RA   LaBaer J.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Cerebellum, and Kidney;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10591208; DOI=10.1038/990031;
RA   Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA   Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA   Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA   Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA   Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA   Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA   Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA   Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA   Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA   Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA   Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA   Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA   Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA   Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA   Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA   Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA   Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA   Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA   Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA   Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA   Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA   Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA   Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA   Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA   Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA   Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA   Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA   Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA   Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA   Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA   Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA   Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA   Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA   McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA   Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA   Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA   Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA   Wright H.;
RT   "The DNA sequence of human chromosome 22.";
RL   Nature 402:489-495(1999).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-303.
RC   TISSUE=Retina;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT   site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
CC   -!- FUNCTION: Transcription factor that binds to and transactivates the
CC       TSHB promoter. Binds to a minimal DNA-binding sequence 5'-
CC       [TC][AG][AG]TTA[TC][AG]-3'.
CC   -!- SUBUNIT: Binds DNA as a homodimer or a heterodimer. Can form a
CC       heterodimer with DBP.
CC   -!- INTERACTION:
CC       Q10587; P50570-2: DNM2; NbExp=3; IntAct=EBI-2796967, EBI-10968534;
CC       Q10587; Q13084: MRPL28; NbExp=3; IntAct=EBI-2796967, EBI-723426;
CC       Q10587; Q16236: NFE2L2; NbExp=5; IntAct=EBI-2796967, EBI-2007911;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q10587-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q10587-2; Sequence=VSP_041376;
CC   -!- INDUCTION: Accumulates according to a robust circadian rhythm.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bZIP family. PAR subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA81373.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U06935; AAA81373.1; ALT_INIT; mRNA.
DR   EMBL; U44059; AAB06497.1; -; mRNA.
DR   EMBL; CR456592; CAG30478.1; -; mRNA.
DR   EMBL; CR541827; CAG46626.1; -; mRNA.
DR   EMBL; AK091916; BAG52441.1; -; mRNA.
DR   EMBL; AK314906; BAG37419.1; -; mRNA.
DR   EMBL; AL035659; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471095; EAW60430.1; -; Genomic_DNA.
DR   EMBL; CH471095; EAW60431.1; -; Genomic_DNA.
DR   EMBL; BC039258; AAH39258.1; -; mRNA.
DR   EMBL; BC042476; AAH42476.1; -; mRNA.
DR   EMBL; BX537848; CAD97856.1; -; mRNA.
DR   CCDS; CCDS14014.1; -. [Q10587-1]
DR   CCDS; CCDS46716.1; -. [Q10587-2]
DR   PIR; G02360; G02360.
DR   RefSeq; NP_001138870.1; NM_001145398.2. [Q10587-2]
DR   RefSeq; NP_003207.1; NM_003216.3. [Q10587-1]
DR   PDB; 4U5T; X-ray; 3.30 A; A/B=257-295.
DR   PDBsum; 4U5T; -.
DR   AlphaFoldDB; Q10587; -.
DR   SMR; Q10587; -.
DR   BioGRID; 112867; 3.
DR   IntAct; Q10587; 33.
DR   STRING; 9606.ENSP00000266304; -.
DR   iPTMnet; Q10587; -.
DR   PhosphoSitePlus; Q10587; -.
DR   BioMuta; TEF; -.
DR   DMDM; 51338730; -.
DR   EPD; Q10587; -.
DR   jPOST; Q10587; -.
DR   MassIVE; Q10587; -.
DR   PaxDb; 9606-ENSP00000266304; -.
DR   PeptideAtlas; Q10587; -.
DR   ProteomicsDB; 58863; -. [Q10587-1]
DR   ProteomicsDB; 58864; -. [Q10587-2]
DR   Antibodypedia; 26917; 191 antibodies from 25 providers.
DR   DNASU; 7008; -.
DR   Ensembl; ENST00000266304.9; ENSP00000266304.4; ENSG00000167074.15. [Q10587-1]
DR   Ensembl; ENST00000406644.7; ENSP00000385256.3; ENSG00000167074.15. [Q10587-2]
DR   GeneID; 7008; -.
DR   KEGG; hsa:7008; -.
DR   MANE-Select; ENST00000266304.9; ENSP00000266304.4; NM_003216.4; NP_003207.1.
DR   UCSC; uc003azx.5; human. [Q10587-1]
DR   AGR; HGNC:11722; -.
DR   CTD; 7008; -.
DR   DisGeNET; 7008; -.
DR   GeneCards; TEF; -.
DR   HGNC; HGNC:11722; TEF.
DR   HPA; ENSG00000167074; Low tissue specificity.
DR   MIM; 188595; gene.
DR   neXtProt; NX_Q10587; -.
DR   OpenTargets; ENSG00000167074; -.
DR   PharmGKB; PA36439; -.
DR   VEuPathDB; HostDB:ENSG00000167074; -.
DR   eggNOG; KOG3119; Eukaryota.
DR   GeneTree; ENSGT00940000156578; -.
DR   HOGENOM; CLU_051922_2_0_1; -.
DR   InParanoid; Q10587; -.
DR   OMA; CCANRIC; -.
DR   OrthoDB; 5354784at2759; -.
DR   PhylomeDB; Q10587; -.
DR   TreeFam; TF315869; -.
DR   PathwayCommons; Q10587; -.
DR   SignaLink; Q10587; -.
DR   SIGNOR; Q10587; -.
DR   BioGRID-ORCS; 7008; 31 hits in 1171 CRISPR screens.
DR   ChiTaRS; TEF; human.
DR   GeneWiki; TEF_(gene); -.
DR   GenomeRNAi; 7008; -.
DR   Pharos; Q10587; Tbio.
DR   PRO; PR:Q10587; -.
DR   Proteomes; UP000005640; Chromosome 22.
DR   RNAct; Q10587; Protein.
DR   Bgee; ENSG00000167074; Expressed in cerebellar hemisphere and 181 other cell types or tissues.
DR   ExpressionAtlas; Q10587; baseline and differential.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR   GO; GO:0044877; F:protein-containing complex binding; IEA:Ensembl.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR   CDD; cd14695; bZIP_HLF; 1.
DR   Gene3D; 1.20.5.170; -; 1.
DR   InterPro; IPR004827; bZIP.
DR   InterPro; IPR046347; bZIP_sf.
DR   InterPro; IPR040223; PAR_bZIP.
DR   PANTHER; PTHR11988:SF24; THYROTROPH EMBRYONIC FACTOR; 1.
DR   PANTHER; PTHR11988; THYROTROPH EMBRYONIC FACTOR RELATED; 1.
DR   Pfam; PF07716; bZIP_2; 1.
DR   SMART; SM00338; BRLZ; 1.
DR   SUPFAM; SSF57959; Leucine zipper domain; 1.
DR   PROSITE; PS50217; BZIP; 1.
DR   Genevisible; Q10587; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Alternative splicing; Biological rhythms;
KW   DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..303
FT                   /note="Thyrotroph embryonic factor"
FT                   /id="PRO_0000076512"
FT   DOMAIN          233..296
FT                   /note="bZIP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   REGION          1..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          132..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          235..255
FT                   /note="Basic motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   REGION          256..263
FT                   /note="Leucine-zipper"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   COMPBIAS        44..63
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        132..158
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         32
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:20068231"
FT   VAR_SEQ         1..52
FT                   /note="MSDAGGGKKPPVDPQAGPGPGPGRAAGERGLSGSFPLVLKKLMENPPREARL
FT                   -> MDMPEVLKSLLEHSLPWPEKRT (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_041376"
FT   CONFLICT        54
FT                   /note="K -> E (in Ref. 1; AAA81373)"
FT                   /evidence="ECO:0000305"
FT   HELIX           261..293
FT                   /evidence="ECO:0007829|PDB:4U5T"
SQ   SEQUENCE   303 AA;  33248 MW;  4A87B7BFA7248C6F CRC64;
     MSDAGGGKKP PVDPQAGPGP GPGRAAGERG LSGSFPLVLK KLMENPPREA RLDKEKGKEK
     LEEDEAAAAS TMAVSASLMP PIWDKTIPYD GESFHLEYMD LDEFLLENGI PASPTHLAHN
     LLLPVAELEG KESASSSTAS PPSSSTAIFQ PSETVSSTES SLEKERETPS PIDPNCVEVD
     VNFNPDPADL VLSSVPGGEL FNPRKHKFAE EDLKPQPMIK KAKKVFVPDE QKDEKYWTRR
     KKNNVAAKRS RDARRLKENQ ITIRAAFLEK ENTALRTEVA ELRKEVGKCK TIVSKYETKY
     GPL
//