Q10587 (TEF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thyrotroph embryonic factor

Gene names

Name:	TEF
Synonyms:	KIAA1655

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3'.
Subunit structure	Binds DNA as a homodimer or a heterodimer. Can form a heterodimer with DBP.
Subcellular location	Nucleus.
Induction	Accumulates according to a robust circadian rhythm By similarity.
Sequence similarities	Belongs to the bZIP family. PAR subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Sequence caution	The sequence AAA81373.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Biological rhythms Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q10587-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q10587-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: MSDAGGGKKPPVDPQAGPGPGPGRAAGERGLSGSFPLVLKKLMENPPREARL → MDMPEVLKSLLEHSLPWPEKRT

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Thyrotroph embryonic factor		PRO_0000076512
Regions
	Domain	233 – 296	64	bZIP
	Region	235 – 255	21	Basic motif By similarity
	Region	256 – 263	8	Leucine-zipper By similarity
	Compositional bias	166 – 217	52	Pro-rich (proline/acidic region (PAR))
Amino acid modifications
	Modified residue	32	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	1 – 52	52	MSDAG…REARL → MDMPEVLKSLLEHSLPWPEK RT in isoform 2.		VSP_041376
Experimental info
	Sequence conflict	54	1	K → E in AAA81373. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: 4A87B7BFA7248C6F	FASTA	303	33,248
	10 20 30 40 50 60 MSDAGGGKKP PVDPQAGPGP GPGRAAGERG LSGSFPLVLK KLMENPPREA RLDKEKGKEK 70 80 90 100 110 120 LEEDEAAAAS TMAVSASLMP PIWDKTIPYD GESFHLEYMD LDEFLLENGI PASPTHLAHN 130 140 150 160 170 180 LLLPVAELEG KESASSSTAS PPSSSTAIFQ PSETVSSTES SLEKERETPS PIDPNCVEVD 190 200 210 220 230 240 VNFNPDPADL VLSSVPGGEL FNPRKHKFAE EDLKPQPMIK KAKKVFVPDE QKDEKYWTRR 250 260 270 280 290 300 KKNNVAAKRS RDARRLKENQ ITIRAAFLEK ENTALRTEVA ELRKEVGKCK TIVSKYETKY GPL
	Isoform 2 [UniParc]. Checksum: B1A956839CBC4AC4	FASTA	273	30,635
	10 20 30 40 50 60 MDMPEVLKSL LEHSLPWPEK RTDKEKGKEK LEEDEAAAAS TMAVSASLMP PIWDKTIPYD 70 80 90 100 110 120 GESFHLEYMD LDEFLLENGI PASPTHLAHN LLLPVAELEG KESASSSTAS PPSSSTAIFQ 130 140 150 160 170 180 PSETVSSTES SLEKERETPS PIDPNCVEVD VNFNPDPADL VLSSVPGGEL FNPRKHKFAE 190 200 210 220 230 240 EDLKPQPMIK KAKKVFVPDE QKDEKYWTRR KKNNVAAKRS RDARRLKENQ ITIRAAFLEK 250 260 270 ENTALRTEVA ELRKEVGKCK TIVSKYETKY GPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chromosomal localization and cDNA cloning of the human DBP and TEF genes." Khatib Z.A., Inaba T., Valentine M., Look A.T. Genomics 23:344-351(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The proto-oncogene HLF and the related basic leucine zipper protein TEF display highly similar DNA-binding and transcriptional regulatory properties." Hunger S.P., Li S., Fall M.Z., Naumovski L., Cleary M.L. Blood 87:4607-4617(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Cerebellum and Kidney.
[6]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[9]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-303. Tissue: Retina.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06935 mRNA. Translation: AAA81373.1. Different initiation. U44059 mRNA. Translation: AAB06497.1. CR456592 mRNA. Translation: CAG30478.1. CR541827 mRNA. Translation: CAG46626.1. AK091916 mRNA. Translation: BAG52441.1. AK314906 mRNA. Translation: BAG37419.1. AL035659 Genomic DNA. Translation: CAI23516.1. AL035659 Genomic DNA. Translation: CAQ08872.1. CH471095 Genomic DNA. Translation: EAW60430.1. CH471095 Genomic DNA. Translation: EAW60431.1. BC039258 mRNA. Translation: AAH39258.1. BC042476 mRNA. Translation: AAH42476.1. BX537848 mRNA. Translation: CAD97856.1.
IPI	IPI00216670. IPI00879207.
PIR	G02360.
RefSeq	NP_001138870.1. NM_001145398.2. NP_003207.1. NM_003216.3.
UniGene	Hs.181159.
3D structure databases
ProteinModelPortal	Q10587.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10587. 29 interactions.
STRING	9606.ENSP00000266304.
PTM databases
PhosphoSite	Q10587.
Polymorphism databases
DMDM	51338730.
Proteomic databases
PaxDb	Q10587.
PRIDE	Q10587.
Protocols and materials databases
DNASU	7008.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000266304; ENSP00000266304; ENSG00000167074. ENST00000406644; ENSP00000385256; ENSG00000167074. ENST00000433913; ENSP00000400221; ENSG00000167074.
GeneID	7008.
KEGG	hsa:7008.
UCSC	uc003azx.3. human. uc003azy.3. human.
Organism-specific databases
CTD	7008.
GeneCards	GC22P041763.
HGNC	HGNC:11722. TEF.
HPA	HPA028868.
MIM	188595. gene.
neXtProt	NX_Q10587.
PharmGKB	PA36439.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG323431.
HOVERGEN	HBG004983.
InParanoid	Q10587.
KO	K09058.
OMA	ETRYGPF.
OrthoDB	EOG444KM8.
PhylomeDB	Q10587.
Gene expression databases
ArrayExpress	Q10587.
Bgee	Q10587.
CleanEx	HS_TEF.
Genevestigator	Q10587.
GermOnline	ENSG00000167074. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TEF. human.
GenomeRNAi	7008.
NextBio	27374.
SOURCE	Search...

Entry information

Entry name

TEF_HUMAN

Accession

Primary (citable) accession number: Q10587
Secondary accession number(s): B0QYS8 , B2RC22, Q15729, Q7Z3J7, Q8IU94, Q96TG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.