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Protein

Thyrotroph embryonic factor

Gene

TEF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3'.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ10587.

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotroph embryonic factor
Gene namesi
Name:TEF
Synonyms:KIAA1655
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:11722. TEF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36439.

Polymorphism and mutation databases

BioMutaiTEF.
DMDMi51338730.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Thyrotroph embryonic factorPRO_0000076512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ10587.
PRIDEiQ10587.

PTM databases

PhosphoSiteiQ10587.

Expressioni

Inductioni

Accumulates according to a robust circadian rhythm.By similarity

Gene expression databases

BgeeiQ10587.
CleanExiHS_TEF.
ExpressionAtlasiQ10587. baseline and differential.
GenevisibleiQ10587. HS.

Organism-specific databases

HPAiHPA028868.

Interactioni

Subunit structurei

Binds DNA as a homodimer or a heterodimer. Can form a heterodimer with DBP.

Binary interactionsi

WithEntry#Exp.IntActNotes
MRPL28Q130843EBI-2796967,EBI-723426

Protein-protein interaction databases

BioGridi112867. 1 interaction.
IntActiQ10587. 31 interactions.
STRINGi9606.ENSP00000266304.

Structurei

3D structure databases

ProteinModelPortaliQ10587.
SMRiQ10587. Positions 261-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini233 – 29664bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni235 – 25521Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni256 – 2638Leucine-zipperPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi166 – 21752Pro-rich (proline/acidic region (PAR))Add
BLAST

Sequence similaritiesi

Belongs to the bZIP family. PAR subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG323431.
GeneTreeiENSGT00440000039070.
HOVERGENiHBG004983.
InParanoidiQ10587.
KOiK09058.
OMAiCKNIVSK.
OrthoDBiEOG712TZJ.
PhylomeDBiQ10587.
TreeFamiTF315869.

Family and domain databases

InterProiIPR004827. bZIP.
IPR029832. TEF/VBP.
[Graphical view]
PANTHERiPTHR11988:SF24. PTHR11988:SF24. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q10587-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDAGGGKKP PVDPQAGPGP GPGRAAGERG LSGSFPLVLK KLMENPPREA
60 70 80 90 100
RLDKEKGKEK LEEDEAAAAS TMAVSASLMP PIWDKTIPYD GESFHLEYMD
110 120 130 140 150
LDEFLLENGI PASPTHLAHN LLLPVAELEG KESASSSTAS PPSSSTAIFQ
160 170 180 190 200
PSETVSSTES SLEKERETPS PIDPNCVEVD VNFNPDPADL VLSSVPGGEL
210 220 230 240 250
FNPRKHKFAE EDLKPQPMIK KAKKVFVPDE QKDEKYWTRR KKNNVAAKRS
260 270 280 290 300
RDARRLKENQ ITIRAAFLEK ENTALRTEVA ELRKEVGKCK TIVSKYETKY

GPL
Length:303
Mass (Da):33,248
Last modified:August 16, 2004 - v3
Checksum:i4A87B7BFA7248C6F
GO
Isoform 2 (identifier: Q10587-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: MSDAGGGKKPPVDPQAGPGPGPGRAAGERGLSGSFPLVLKKLMENPPREARL → MDMPEVLKSLLEHSLPWPEKRT

Note: No experimental confirmation available.
Show »
Length:273
Mass (Da):30,635
Checksum:iB1A956839CBC4AC4
GO

Sequence cautioni

The sequence AAA81373.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541K → E in AAA81373 (PubMed:7835883).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5252MSDAG…REARL → MDMPEVLKSLLEHSLPWPEK RT in isoform 2. 1 PublicationVSP_041376Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06935 mRNA. Translation: AAA81373.1. Different initiation.
U44059 mRNA. Translation: AAB06497.1.
CR456592 mRNA. Translation: CAG30478.1.
CR541827 mRNA. Translation: CAG46626.1.
AK091916 mRNA. Translation: BAG52441.1.
AK314906 mRNA. Translation: BAG37419.1.
AL035659 Genomic DNA. Translation: CAI23516.1.
AL035659 Genomic DNA. Translation: CAQ08872.1.
CH471095 Genomic DNA. Translation: EAW60430.1.
CH471095 Genomic DNA. Translation: EAW60431.1.
BC039258 mRNA. Translation: AAH39258.1.
BC042476 mRNA. Translation: AAH42476.1.
BX537848 mRNA. Translation: CAD97856.1.
CCDSiCCDS14014.1. [Q10587-1]
CCDS46716.1. [Q10587-2]
PIRiG02360.
RefSeqiNP_001138870.1. NM_001145398.2. [Q10587-2]
NP_003207.1. NM_003216.3. [Q10587-1]
UniGeneiHs.181159.

Genome annotation databases

EnsembliENST00000266304; ENSP00000266304; ENSG00000167074. [Q10587-1]
ENST00000406644; ENSP00000385256; ENSG00000167074. [Q10587-2]
GeneIDi7008.
KEGGihsa:7008.
UCSCiuc003azx.4. human. [Q10587-2]
uc003azy.4. human. [Q10587-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06935 mRNA. Translation: AAA81373.1. Different initiation.
U44059 mRNA. Translation: AAB06497.1.
CR456592 mRNA. Translation: CAG30478.1.
CR541827 mRNA. Translation: CAG46626.1.
AK091916 mRNA. Translation: BAG52441.1.
AK314906 mRNA. Translation: BAG37419.1.
AL035659 Genomic DNA. Translation: CAI23516.1.
AL035659 Genomic DNA. Translation: CAQ08872.1.
CH471095 Genomic DNA. Translation: EAW60430.1.
CH471095 Genomic DNA. Translation: EAW60431.1.
BC039258 mRNA. Translation: AAH39258.1.
BC042476 mRNA. Translation: AAH42476.1.
BX537848 mRNA. Translation: CAD97856.1.
CCDSiCCDS14014.1. [Q10587-1]
CCDS46716.1. [Q10587-2]
PIRiG02360.
RefSeqiNP_001138870.1. NM_001145398.2. [Q10587-2]
NP_003207.1. NM_003216.3. [Q10587-1]
UniGeneiHs.181159.

3D structure databases

ProteinModelPortaliQ10587.
SMRiQ10587. Positions 261-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112867. 1 interaction.
IntActiQ10587. 31 interactions.
STRINGi9606.ENSP00000266304.

PTM databases

PhosphoSiteiQ10587.

Polymorphism and mutation databases

BioMutaiTEF.
DMDMi51338730.

Proteomic databases

PaxDbiQ10587.
PRIDEiQ10587.

Protocols and materials databases

DNASUi7008.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000266304; ENSP00000266304; ENSG00000167074. [Q10587-1]
ENST00000406644; ENSP00000385256; ENSG00000167074. [Q10587-2]
GeneIDi7008.
KEGGihsa:7008.
UCSCiuc003azx.4. human. [Q10587-2]
uc003azy.4. human. [Q10587-1]

Organism-specific databases

CTDi7008.
GeneCardsiGC22P041763.
HGNCiHGNC:11722. TEF.
HPAiHPA028868.
MIMi188595. gene.
neXtProtiNX_Q10587.
PharmGKBiPA36439.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG323431.
GeneTreeiENSGT00440000039070.
HOVERGENiHBG004983.
InParanoidiQ10587.
KOiK09058.
OMAiCKNIVSK.
OrthoDBiEOG712TZJ.
PhylomeDBiQ10587.
TreeFamiTF315869.

Enzyme and pathway databases

SignaLinkiQ10587.

Miscellaneous databases

ChiTaRSiTEF. human.
GeneWikiiTEF_(gene).
GenomeRNAii7008.
NextBioi27374.
PROiQ10587.
SOURCEiSearch...

Gene expression databases

BgeeiQ10587.
CleanExiHS_TEF.
ExpressionAtlasiQ10587. baseline and differential.
GenevisibleiQ10587. HS.

Family and domain databases

InterProiIPR004827. bZIP.
IPR029832. TEF/VBP.
[Graphical view]
PANTHERiPTHR11988:SF24. PTHR11988:SF24. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Chromosomal localization and cDNA cloning of the human DBP and TEF genes."
    Khatib Z.A., Inaba T., Valentine M., Look A.T.
    Genomics 23:344-351(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The proto-oncogene HLF and the related basic leucine zipper protein TEF display highly similar DNA-binding and transcriptional regulatory properties."
    Hunger S.P., Li S., Fall M.Z., Naumovski L., Cleary M.L.
    Blood 87:4607-4617(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Kidney.
  6. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-303.
    Tissue: Retina.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiTEF_HUMAN
AccessioniPrimary (citable) accession number: Q10587
Secondary accession number(s): B0QYS8
, B2RC22, Q15729, Q7Z3J7, Q8IU94, Q96TG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: August 16, 2004
Last modified: June 24, 2015
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.