Q10585 (SPD1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-phase delaying protein 1

Alternative name(s):
Protein p14

Gene names

Name:	spd1
ORF Names:	SPAC29B12.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the ribonucleotide reductase activity through its mediation of the nuclear localization of suc22, the small subunit of the ribonucleotide reductase. Delays the progression of the G1-S phase transition, thereby ensuring the G1 phase is complete. Interacts with both p34 and the p34-p56 complex, although no direct inhibitory effect on the bound proteins has been demonstrated. The action of p14 may happen coincidentally with the cdc10 function or may happen downstream of this. Ref.1 Ref.3 Ref.4 Ref.7
Subunit structure	Interacts with cdc22. Ref.1 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.3 Ref.8.
Developmental stage	The levels peak in the G1 phase of the cell cycle, then mostly disappear during S phase and reappear in the G2 phase.
Post-translational modification	Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation. Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the DIF1/spd1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular protein localization Inferred from genetic interaction. Source: GeneDB_Spombe mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic cell cycle DNA replication checkpoint Traceable author statement. Source: GeneDB_Spombe negative regulation of S phase of mitotic cell cycle Inferred from genetic interaction. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	enzyme inhibitor activity Inferred from direct assay. Source: GeneDB_Spombe protein anchor Inferred from genetic interaction. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	S-phase delaying protein 1		PRO_0000072110

Sequences

	Sequence		Length	Mass (Da)
	Q10585 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F43A6C9D86F55F51	FASTA	124	14,225
	10 20 30 40 50 60 MHSSKRVMTT KTHVEQPESS MRPQLPESIQ GSLMDVGMRV RKSISTGYKS KQTTFPAYNP 70 80 90 100 110 120 PLYNTVSENI ALKNTAFSYE PNGTKRPFEQ AIPNYNWANP PQDFEEPEWL KPFDVVMEGT NERL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel S phase inhibitor in fission yeast." Woollard A., Basi G., Nurse P. EMBO J. 15:4603-4612(1996) [PubMed: 8887552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH CDC22. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast." Borgne A., Nurse P. J. Cell Sci. 113:4341-4350(2000) [PubMed: 11069778] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms." Liu C., Powell K.A., Mundt K., Wu L., Carr A.M., Caspari T. Genes Dev. 17:1130-1140(2003) [PubMed: 12695334] [Abstract] Cited for: FUNCTION, UBIQUITINATION.
[5]	"Ddb1 is required for the proteolysis of the Schizosaccharomyces pombe replication inhibitor Spd1 during S phase and after DNA damage." Bondar T., Ponomarev A., Raychaudhuri P. J. Biol. Chem. 279:9937-9943(2004) [PubMed: 14701809] [Abstract] Cited for: UBIQUITINATION.
[6]	"Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase." Liu C., Poitelea M., Watson A., Yoshida S.H., Shimoda C., Holmberg C., Nielsen O., Carr A.M. EMBO J. 24:3940-3951(2005) [PubMed: 16252005] [Abstract] Cited for: UBIQUITINATION.
[7]	"The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit." Hakansson P., Dahl L., Chilkova O., Domkin V., Thelander L. J. Biol. Chem. 281:1778-1783(2006) [PubMed: 16317005] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC22.
[8]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98361 Genomic DNA. Translation: CAA67006.1. CU329670 Genomic DNA. Translation: CAB16248.1.
PIR	S71888.
RefSeq	NP_594981.1. NM_001020412.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10585. 1 interaction.
STRING	Q10585.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC29B12.03.1; SPAC29B12.03.1:pep; SPAC29B12.03.
GeneID	2542696.
GenomeReviews	Gene locus spd1 in contig CU329670_GR.
KEGG	spo:SPAC29B12.03.
NMPDR	fig|4896.1.peg.4951.
Organism-specific databases
GeneDB_Spombe	SPAC29B12.03.
Phylogenomic databases
eggNOG	fuNOG13199.
OrthoDB	EOG46HKM7.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003040-MONOMER.
Gene expression databases
ArrayExpress	Q10585.
Family and domain databases
InterPro	IPR013900. RNR_inhibitor. [Graphical view]
Pfam	PF08591. RNR_inhib. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPD1_SCHPO

Accession

Primary (citable) accession number: Q10585

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program