Reviewed, UniProtKB/Swiss-Prot Q10585 (SPD1_SCHPO)

Names and origin

Protein names

Recommended name:
    S-phase delaying protein 1
Alternative name(s):
    Protein p14

Gene names

Name:	spd1
ORF Names:	SPAC29B12.03

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Delays the progression of the G1-S phase transition, thereby ensuring the G1 phase is complete. Interacts with both p34 and the p34-p56 complex, although no direct inhibitory effect on the bound proteins has been demonstrated. The action of p14 may happen coincidentally with the cdc10 function or may happen downstream of this.
Developmental stage	The levels peak in the G1 phase of the cell cycle, then mostly disappear during S phase and reappear in the G2 phase.
Post-translational modification	Ubiquitinated. Ubiquitination requires ddb1 and leads to subsequent proteolysis. Ref.3

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication checkpoint Traceable author statement. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular protein localization Inferred from genetic interaction. Source: GeneDB_SPombe mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of S phase of mitotic cell cycle Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	enzyme inhibitor activity Inferred from direct assay. Source: GeneDB_SPombe protein anchor Inferred from genetic interaction. Source: GeneDB_SPombe
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc22	P36602	1	EBI-969142,EBI-969154

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	S-phase delaying protein 1		PRO_0000072110

Sequences

	Sequence		Length	Mass (Da)
	Q10585-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F43A6C9D86F55F51	FASTA	124	14,225
	10 20 30 40 50 60 MHSSKRVMTT KTHVEQPESS MRPQLPESIQ GSLMDVGMRV RKSISTGYKS KQTTFPAYNP 70 80 90 100 110 120 PLYNTVSENI ALKNTAFSYE PNGTKRPFEQ AIPNYNWANP PQDFEEPEWL KPFDVVMEGT NERL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel S phase inhibitor in fission yeast." Woollard A., Basi G., Nurse P. EMBO J. 15:4603-4612(1996) [PubMed: 8887552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Ddb1 is required for the proteolysis of the Schizosaccharomyces pombe replication inhibitor Spd1 during S phase and after DNA damage." Bondar T., Ponomarev A., Raychaudhuri P. J. Biol. Chem. 279:9937-9943(2004) [PubMed: 14701809] [Abstract] Cited for: UBIQUITINATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X98361 Genomic DNA. Translation: CAA67006.1. CU329670 Genomic DNA. Translation: CAB16248.1.
PIR	S71888.
RefSeq	NP_594981.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10585. 1 interaction.
STRING	Q10585.
Genome annotation databases
GeneID	2542696.
GenomeReviews	Gene locus spd1 in contig CU329670_GR.
KEGG	spo:SPAC29B12.03.
NMPDR	fig|4896.1.peg.4951.
Organism-specific databases
GeneDB_Spombe	SPAC29B12.03.
Phylogenomic databases
OrthoDB	EOG97PZP7.
Gene expression databases
ArrayExpress	Q10585.
Family and domain databases
InterPro	IPR013900. RNR_inhibitor. [Graphical view]
Pfam	PF08591. RNR_inhib. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPD1_SCHPO

Accession

Primary (citable) accession number: Q10585

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)