Reviewed, UniProtKB/Swiss-Prot Q10578 (RPB2_CAEEL)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase II subunit RPB2
      Short name=RNA polymerase II subunit B2
      Short name=RNA polymerase II subunit 2
    EC=2.7.7.6

Gene names

Name:	rbp-2
ORF Names:	C26E6.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	determination of adult life span Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1193	1193	DNA-directed RNA polymerase II subunit RPB2		PRO_0000048083
Regions
	Zinc finger	1125 – 1146	22	C4-type
Sites
	Metal binding	798	1	Magnesium; shared with RPB1 By similarity
	Metal binding	1125	1	Zinc By similarity
	Metal binding	1128	1	Zinc By similarity
	Metal binding	1143	1	Zinc By similarity
	Metal binding	1146	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10578-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B8A85E74E9CC7EBE	FASTA	1,193	134,905
	10 20 30 40 50 60 MYDDEDEMVN DPMDGDYIDD SDEISAEAWQ EACWVVISAY FDEKGLVRQQ LDSFDEFVQM 70 80 90 100 110 120 NVQRIVEDSP PVELQSENQH LGTDMENPAK FSLKFNQIYL SKPTHWEKDG APMPMMPNEA 130 140 150 160 170 180 RLRNLTYASP LYVDITKVVT RDDSATEKVY DKVFVGKVPV MLRSSYCMLS NMTDRDLTEL 190 200 210 220 230 240 NECPLDPGGY FVINGSEKVL IAQEKMATNT VYVFSMKDGK YAFKTECRSC LENSSRPTST 250 260 270 280 290 300 MWVNMLARGG GGGKKTAMGQ RIIGILPYIK QEIPIMIVFR ALGFVSDRDI LGHIIYDFND 310 320 330 340 350 360 PEMMEMVKPS LDEAFVIQEQ NVALNFIGAR GAKPGVTREQ RIKYAREILQ KELLPHVGVS 370 380 390 400 410 420 EHCETKKAFF IGYMVHRLLL AALGRRELDD RDHIGNKRLD LAGPLLAFLF RSLFRNLLKE 430 440 450 460 470 480 MRMTAQKYIN KNDDFALDVC VKTSTITRGL TYSLATGNWG DQKKAHQSRA GVSQVLNRLT 490 500 510 520 530 540 YTATLSHLRR ANSPIGREGK LAKPRQLHNT QWGMVCPAET PEGQAVGLVK NLALMAYISV 550 560 570 580 590 600 GSLPEPILEF LEEWSMENLE EVSPSAIADA TKIFVNGAWV GIHREPDQLM TTLKKLRRQM 610 620 630 640 650 660 DIIVSEVSMV RDIRDREIRI YTDAGRVCRP LLIVENQKLA LKKRHIDQLK EAADEANKYT 670 680 690 700 710 720 WSDLVGGGVV ELIDSMEEET SMIAMMPEDL RSGGYCDTHT HCEIHPAMIL GVCASIIPFP 730 740 750 760 770 780 DHNQSPRNTY QSAMGKQAMG VYTTNFHVRM DTLAHVLYYP QKPLVTTRSM EYLRFNELPA 790 800 810 820 830 840 GINAIVAILS YSGYNQEDSV IMNNSAIDRG LFRSVFYRSY RDNEANLDNA NEELIEKPTR 850 860 870 880 890 900 EKCSGMRHSL YDKLDEDGII SPGMRVSGDD VIIGKTVALP DIDDDLDASG KKYPKRDAST 910 920 930 940 950 960 FLRSSETGIV DQVMLSLNSD GNKFVKIRMR SVRLPQIGDK FASRHGQKGT MGIMYRQEDM 970 980 990 1000 1010 1020 PFTAEGLTPD IIINPHAVPS RMTIGHLIEC LQGKLSANKG EIGDATPFND TVNVQKISGL 1030 1040 1050 1060 1070 1080 LCEYGYHLRG NEVMYNGHTG KKLTTQIFFG PTYYQRLKHM VDDKIHSRAR GPIQMMNRQP 1090 1100 1110 1120 1130 1140 MEGRARDGGL RFGEMERDCQ ISHGATQFLR ERLFEVSDPY HVYVCNNCGL IVVANLRTNS 1150 1160 1170 1180 1190 FECKACRNKT QVSAVRIPYA CKLLFQELMS MSIAPRLMVK PRQSKRSKHQ SEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"A molecular evolutionary framework for eukaryotic model organisms." Sidow A., Thomas W.K. Curr. Biol. 4:596-603(1994) [PubMed: 7953533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 200-1058.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13875 Genomic DNA. Translation: AAA21158.1. U10333 mRNA. Translation: AAA50224.1.
PIR	E88445. T43701.
RefSeq	NP_498047.1.
UniGene	Cel.7716
3D structure databases
HSSP	HSSP built from PDB template 1I50 based on UniProtKB P08518.
ModBase	Search...
Genome annotation databases
Ensembl	C26E6.4. Caenorhabditis elegans. [Contig view]
GeneID	175668.
KEGG	cel:C26E6.4.
NMPDR	fig|6239.3.peg.9616.
Organism-specific databases
WormBase	WBGene00016140. rbp-2.
WormPep	C26E6.4. CE01162. [WorfDB]
Phylogenomic databases
OMA	Q10578. FGPTYYQ.
Enzyme and pathway databases
BRENDA	2.7.7.6. 672.
Gene expression databases
ArrayExpress	Q10578.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	889138.

Entry information

Entry name

RPB2_CAEEL

Accession

Primary (citable) accession number: Q10578

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project