Q10574 (LIN32_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein lin-32

Alternative name(s):
Abnormal cell lineage protein 32

Gene names

Name:	lin-32
ORF Names:	T14F9.5

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the specification of the neuroblast cell fate in the development of peripheral sense organs. Ref.1
Subcellular location	Nucleus Ref.1.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.
Sequence caution	The sequence AAA67360.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryo development ending in birth or egg hatching Inferred from mutant phenotype PubMed 11076762. Source: WormBase localization Inferred from mutant phenotype PubMed 8187641. Source: WormBase neuron development Inferred from mutant phenotype PubMed 11076762PubMed 8187641. Source: WormBase tail tip morphogenesis Inferred from mutant phenotype PubMed 11076762. Source: WormBase
Cellular_component	nucleus Inferred by curator PubMed 11076762. Source: WormBase
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 11076762. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 142	142	Protein lin-32		PRO_0000127261
Regions
	Domain	72 – 124	53	bHLH
Experimental info
	Mutagenesis	81	1	E → K in lin-32(U282); 99% ray loss and tail touch insensitivity. Ref.1
	Mutagenesis	95	1	L → F in lin-32(E1926); 81% ray loss. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q10574 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: DA20245666C65E8D	FASTA	142	16,480
	10 20 30 40 50 60 MSWEQYQMYV PQCHPSFMYQ GSIQSTMTTP LQSPNFSLDS PNYPDSLSNG GGKDDKKKCR 70 80 90 100 110 120 RYKTPSPQLL RMRRSAANER ERRRMNTLNV AYDELREVLP EIDSGKKLSK FETLQMAQKY 130 140 IECLSQILKQ DSKNENLKSK SG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A transcription factor controlling development of peripheral sense organs in C. elegans." Zhao C., Emmons S.W. Nature 373:74-78(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-81 AND LEU-95. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15418 Genomic DNA. Translation: AAA67360.1. Sequence problems. FO081076 Genomic DNA. Translation: CCD68944.1.
PIR	T29378.
RefSeq	NP_508410.2. NM_076009.2.
UniGene	Cel.662.
3D structure databases
ProteinModelPortal	Q10574.
SMR	Q10574. Positions 73-129.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10574. 1 interaction.
STRING	6239.T14F9.5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T14F9.5; T14F9.5; T14F9.5.
GeneID	191703.
KEGG	cel:CELE_T14F9.5.
UCSC	T14F9.5. c. elegans.
Organism-specific databases
CTD	191703.
WormBase	T14F9.5; CE33318; WBGene00003018; lin-32.
Phylogenomic databases
eggNOG	NOG267878.
GeneTree	ENSGT00680000099574.
HOGENOM	HOG000020160.
InParanoid	Q10574.
KO	K09083.
OMA	CRRYKTP.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	950028.

Entry information

Entry name

LIN32_CAEEL

Accession

Primary (citable) accession number: Q10574

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project