Reviewed, UniProtKB/Swiss-Prot Q10572 (FOX1_CAEEL)

Names and origin

Protein names

Recommended name:
    Sex determination protein fox-1
Alternative name(s):
    Feminizing locus on X protein 1

Gene names

Name:	fox-1
ORF Names:	T07D1.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	A member of the sex determination pathway involved in making the distinction between one and two X-chromosomes. RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUG-3' elements. Regulates alternative splicing of tissue-specific exons. Promotes binding of sup-12 to target RNA. Ref.1 Ref.3 Ref.4
Subunit structure	Interacts with sup-12. Ref.4
Subcellular location	Nucleus By similarity.
Tissue specificity	Detected in pharynx, intestine and in muscle from vulva and body wall. Widely expressed in early embryos before morphogenesis.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oviposition Inferred from mutant phenotype. Source: WormBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
eya-1	O17670	1	EBI-317821,EBI-311862
pha-4	Q17381	1	EBI-317821,EBI-321384

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Sex determination protein fox-1		PRO_0000081589
Regions
	Domain	153 – 229	77	RRM
	Compositional bias	1 – 122	122	Gln-rich
	Compositional bias	251 – 354	104	Ala-rich
Experimental info
	Sequence conflict	97	1	Q → R in AAA67723. Ref.1
	Sequence conflict	281	1	A → P in AAA67723. Ref.1
	Sequence conflict	336	1	Q → L in AAA67723. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q10572-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 2792E15219454754	FASTA	415	44,339
	10 20 30 40 50 60 MQALYQLSAT GAQQQNQQIP IGLSNSLLYQ QLAAHQQIAA QQHQQQLAVS AAHQTQNNIM 70 80 90 100 110 120 LATSAPSLIN HMENSTDGKV KDDPNSDYDL QLSIQQQLAA AAQAAQMGQT QIGPQIVGQQ 130 140 150 160 170 180 GQPVVATTAG STNGSAAVTQ PDPSTSSGPD GPKRLHVSNI PFRFRDPDLK TMFEKFGVVS 190 200 210 220 230 240 DVEIIFNERG SKGFGFVTME RPQDAERARQ ELHGSMIEGR KIEVNCATAR VHSKKVKPTG 250 260 270 280 290 300 GILDQMNPLM AQSALAAQAQ MNRALLLRSP LVAQSLLGRG AALIPGMQQP AFQLQAALAG 310 320 330 340 350 360 NPLAQLQGQP LLFNAAALQT NALQQSAFGM DPAAVQAALL ANEQARFQLA AAAAQGNEYI 370 380 390 400 410 MYHQAKQQEL PGRIPSSGNA SAFGEQYLSN ALATASLPSY QMNPALRTLN RFTPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans." Hodgkin J., Zellan J.D., Albertson D.G. Development 120:3681-3689(1994) [PubMed: 7821230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: CB1489. Tissue: Embryo.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo." Kuroyanagi H., Kobayashi T., Mitani S., Hagiwara M. Nat. Methods 3:909-915(2006) [PubMed: 17060915] [Abstract] Cited for: FUNCTION.
[4]	"The Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo." Kuroyanagi H., Ohno G., Mitani S., Hagiwara M. Mol. Cell. Biol. 27:8612-8621(2007) [PubMed: 17923701] [Abstract] Cited for: FUNCTION, INTERACTION WITH SUP-12, TISSUE-SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U14946 mRNA. Translation: AAA67723.1. U41531 Genomic DNA. Translation: AAA83161.2.
PIR	T16825.
RefSeq	NP_508446.1.
UniGene	Cel.6361
3D structure databases
HSSP	HSSP built from PDB template 2MST based on UniProtKB Q61474.
SMR	Q10572. Positions 152-240.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10572. 2 interactions.
Genome annotation databases
Ensembl	T07D1.4. Caenorhabditis elegans. [Contig view]
GeneID	180549.
KEGG	cel:T07D1.4.
Organism-specific databases
WormBase	WBGene00001484. fox-1.
WormPep	T07D1.4. CE25105. [WorfDB]
Phylogenomic databases
OMA	Q10572. VNCATAR.
Gene expression databases
ArrayExpress	Q10572.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	909832.

Entry information

Entry name

FOX1_CAEEL

Accession

Primary (citable) accession number: Q10572
Secondary accession number(s): Q22304

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project