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Protein

AP-1 complex subunit beta-1

Gene

AP1B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Molecular functioni

  • protein kinase binding Source: ParkinsonsUK-UCL
  • protein transporter activity Source: InterPro
  • transporter activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100280-MONOMER.
ReactomeiR-HSA-164940. Nef mediated downregulation of MHC class I complex cell surface expression.
R-HSA-2132295. MHC class II antigen presentation.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit beta-1
Alternative name(s):
Adaptor protein complex AP-1 subunit beta-1
Adaptor-related protein complex 1 subunit beta-1
Beta-1-adaptin
Beta-adaptin 1
Clathrin assembly protein complex 1 beta large chain
Golgi adaptor HA1/AP1 adaptin beta subunit
Gene namesi
Name:AP1B1
Synonyms:ADTB1, BAM22, CLAPB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:554. AP1B1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi162.
PharmGKBiPA24844.

Polymorphism and mutation databases

BioMutaiAP1B1.
DMDMi290457628.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937381 – 949AP-1 complex subunit beta-1Add BLAST949

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei318N6-acetyllysineCombined sources1
Modified residuei574Nitrated tyrosineBy similarity1

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

EPDiQ10567.
MaxQBiQ10567.
PaxDbiQ10567.
PeptideAtlasiQ10567.
PRIDEiQ10567.

PTM databases

iPTMnetiQ10567.
PhosphoSitePlusiQ10567.
SwissPalmiQ10567.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSG00000100280.
CleanExiHS_AP1B1.
ExpressionAtlasiQ10567. baseline and differential.
GenevisibleiQ10567. HS.

Organism-specific databases

HPAiHPA065226.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Binary interactionsi

WithEntry#Exp.IntActNotes
Ap1m1P355855EBI-1171303,EBI-1040251From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106671. 74 interactors.
DIPiDIP-24207N.
IntActiQ10567. 56 interactors.
MINTiMINT-1540570.
STRINGi9606.ENSP00000350199.

Structurei

Secondary structure

1949
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 23Combined sources9
Helixi27 – 42Combined sources16
Helixi48 – 50Combined sources3
Helixi51 – 55Combined sources5
Helixi63 – 76Combined sources14
Turni77 – 79Combined sources3
Helixi83 – 87Combined sources5
Helixi88 – 94Combined sources7
Helixi100 – 110Combined sources11
Helixi116 – 130Combined sources15
Helixi135 – 151Combined sources17
Helixi153 – 159Combined sources7
Helixi161 – 168Combined sources8
Helixi174 – 190Combined sources17
Beta strandi191 – 193Combined sources3
Helixi201 – 213Combined sources13
Helixi216 – 228Combined sources13
Helixi234 – 244Combined sources11
Helixi245 – 249Combined sources5
Helixi253 – 266Combined sources14
Helixi267 – 269Combined sources3
Helixi275 – 290Combined sources16
Helixi291 – 293Combined sources3
Helixi296 – 312Combined sources17
Turni314 – 319Combined sources6
Helixi321 – 324Combined sources4
Helixi332 – 345Combined sources14
Turni348 – 350Combined sources3
Helixi351 – 361Combined sources11
Helixi367 – 383Combined sources17
Helixi387 – 399Combined sources13
Helixi404 – 420Combined sources17
Helixi428 – 431Combined sources4
Helixi432 – 434Combined sources3
Helixi442 – 454Combined sources13
Turni455 – 458Combined sources4
Helixi462 – 467Combined sources6
Turni471 – 475Combined sources5
Helixi478 – 494Combined sources17
Helixi500 – 511Combined sources12
Helixi517 – 532Combined sources16
Helixi534 – 540Combined sources7
Helixi557 – 564Combined sources8
Turni565 – 568Combined sources4
Helixi570 – 574Combined sources5
Helixi578 – 580Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HMYX-ray7.00B1-584[»]
4P6ZX-ray3.00B1-584[»]
ProteinModelPortaliQ10567.
SMRiQ10567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi576 – 725Pro-rich (stalk region)Add BLAST150

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
HOGENOMiHOG000163270.
HOVERGENiHBG050515.
InParanoidiQ10567.
KOiK12392.
OrthoDBiEOG091G019U.
PhylomeDBiQ10567.
TreeFamiTF300318.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1150. 1 hit.
3.30.310.10. 1 hit.
InterProiIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00185. ARM. 2 hits.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform A (identifier: Q10567-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL
60 70 80 90 100
FPDVVNCMQT DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP
110 120 130 140 150
LIRALAVRTM GCIRVDKITE YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD
160 170 180 190 200
INAQLVEDQG FLDTLKDLIS DSNPMVVANA VAALSEIAES HPSSNLLDLN
210 220 230 240 250
PQSINKLLTA LNECTEWGQI FILDCLANYM PKDDREAQSI CERVTPRLSH
260 270 280 290 300
ANSAVVLSAV KVLMKFMEML SKDLDYYGTL LKKLAPPLVT LLSAEPELQY
310 320 330 340 350
VALRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN
360 370 380 390 400
IAQVLAELKE YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ
410 420 430 440 450
TKVNYVVQEA IVVIKDIFRK YPNKYESVIA TLCENLDSLD EPEARAAMIW
460 470 480 490 500
IVGEYAERID NADELLESFL EGFHDESTQV QLQLLTAIVK LFLKKPTETQ
510 520 530 540 550
ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV VLAEKPLISE
560 570 580 590 600
ETDLIEPTLL DELICYIGTL ASVYHKPPSA FVEGGRGVVH KSLPPRTASS
610 620 630 640 650
ESAESPETAP TGAPPGEQPD VIPAQGDLLG DLLNLDLGPP VSGPPLATSS
660 670 680 690 700
VQMGAVDLLG GGLDSLMGDE PEGIGGTNFV APPTAAVPAN LGAPIGSGLS
710 720 730 740 750
DLFDLTSGVG TLSGSYVAPK AVWLPAMKAK GLEISGTFTR QVGSISMDLQ
760 770 780 790 800
LTNKALQVMT DFAIQFNRNS FGLAPATPLQ VHAPLSPNQT VEISLPLSTV
810 820 830 840 850
GSVMKMEPLN NLQVAVKNNI DVFYFSTLYP LHILFVEDGK MDRQMFLATW
860 870 880 890 900
KDIPNENEAQ FQIRDCPLNA EAASSKLQSS NIFTVAKRNV EGQDMLYQSL
910 920 930 940
KLTNGIWVLA ELRIQPGNPS CTDLELSLKC RAPEVSQHVY QAYETILKN
Length:949
Mass (Da):104,637
Last modified:March 2, 2010 - v2
Checksum:iFE1BA762F9318585
GO
Isoform B (identifier: Q10567-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.

Show »
Length:942
Mass (Da):103,921
Checksum:i2DB97E5FBB283A38
GO
Isoform C (identifier: Q10567-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.
     923-925: Missing.

Show »
Length:939
Mass (Da):103,564
Checksum:iD8817293C15BD8E4
GO
Isoform 4 (identifier: Q10567-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.
     722-741: Missing.
     923-925: Missing.

Note: No experimental confirmation available.
Show »
Length:919
Mass (Da):101,348
Checksum:iAC046601DF0170D3
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_062816777T → A.4 PublicationsCorresponds to variant rs2857465dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000163667 – 673Missing in isoform B, isoform C and isoform 4. 3 Publications7
Alternative sequenceiVSP_044928722 – 741Missing in isoform 4. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_038753923 – 925Missing in isoform C and isoform 4. 2 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13939 mRNA. Translation: AAC98702.1.
CT841508 mRNA. Translation: CAJ86438.1.
AC000041 Genomic DNA. No translation available.
AC002059 Genomic DNA. No translation available.
BC046242 mRNA. Translation: AAH46242.1.
U36268
, U36250, U36251, U36252, U36253, U36254, U36255, U36256, U36257, U36258, U36259, U36260, U36261, U36262, U36263, U36264, U36265, U36266, U36267, AF379038, AF379039 Genomic DNA. Translation: AAC50684.2.
L48038 Genomic DNA. No translation available.
CCDSiCCDS13855.1. [Q10567-1]
CCDS13856.2. [Q10567-3]
CCDS54515.1. [Q10567-4]
PIRiI54360.
RefSeqiNP_001118.3. NM_001127.3.
NP_001159491.1. NM_001166019.1.
NP_663782.2. NM_145730.2.
UniGeneiHs.368794.

Genome annotation databases

EnsembliENST00000317368; ENSP00000319361; ENSG00000100280.
ENST00000357586; ENSP00000350199; ENSG00000100280.
ENST00000402502; ENSP00000386071; ENSG00000100280.
ENST00000405198; ENSP00000384194; ENSG00000100280.
ENST00000415447; ENSP00000387612; ENSG00000100280.
ENST00000432560; ENSP00000400065; ENSG00000100280.
GeneIDi162.
KEGGihsa:162.
UCSCiuc003afi.4. human. [Q10567-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13939 mRNA. Translation: AAC98702.1.
CT841508 mRNA. Translation: CAJ86438.1.
AC000041 Genomic DNA. No translation available.
AC002059 Genomic DNA. No translation available.
BC046242 mRNA. Translation: AAH46242.1.
U36268
, U36250, U36251, U36252, U36253, U36254, U36255, U36256, U36257, U36258, U36259, U36260, U36261, U36262, U36263, U36264, U36265, U36266, U36267, AF379038, AF379039 Genomic DNA. Translation: AAC50684.2.
L48038 Genomic DNA. No translation available.
CCDSiCCDS13855.1. [Q10567-1]
CCDS13856.2. [Q10567-3]
CCDS54515.1. [Q10567-4]
PIRiI54360.
RefSeqiNP_001118.3. NM_001127.3.
NP_001159491.1. NM_001166019.1.
NP_663782.2. NM_145730.2.
UniGeneiHs.368794.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HMYX-ray7.00B1-584[»]
4P6ZX-ray3.00B1-584[»]
ProteinModelPortaliQ10567.
SMRiQ10567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106671. 74 interactors.
DIPiDIP-24207N.
IntActiQ10567. 56 interactors.
MINTiMINT-1540570.
STRINGi9606.ENSP00000350199.

PTM databases

iPTMnetiQ10567.
PhosphoSitePlusiQ10567.
SwissPalmiQ10567.

Polymorphism and mutation databases

BioMutaiAP1B1.
DMDMi290457628.

Proteomic databases

EPDiQ10567.
MaxQBiQ10567.
PaxDbiQ10567.
PeptideAtlasiQ10567.
PRIDEiQ10567.

Protocols and materials databases

DNASUi162.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317368; ENSP00000319361; ENSG00000100280.
ENST00000357586; ENSP00000350199; ENSG00000100280.
ENST00000402502; ENSP00000386071; ENSG00000100280.
ENST00000405198; ENSP00000384194; ENSG00000100280.
ENST00000415447; ENSP00000387612; ENSG00000100280.
ENST00000432560; ENSP00000400065; ENSG00000100280.
GeneIDi162.
KEGGihsa:162.
UCSCiuc003afi.4. human. [Q10567-1]

Organism-specific databases

CTDi162.
DisGeNETi162.
GeneCardsiAP1B1.
HGNCiHGNC:554. AP1B1.
HPAiHPA065226.
MIMi600157. gene.
neXtProtiNX_Q10567.
PharmGKBiPA24844.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
HOGENOMiHOG000163270.
HOVERGENiHBG050515.
InParanoidiQ10567.
KOiK12392.
OrthoDBiEOG091G019U.
PhylomeDBiQ10567.
TreeFamiTF300318.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100280-MONOMER.
ReactomeiR-HSA-164940. Nef mediated downregulation of MHC class I complex cell surface expression.
R-HSA-2132295. MHC class II antigen presentation.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiAP1B1. human.
GeneWikiiAP1B1.
GenomeRNAii162.
PROiQ10567.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100280.
CleanExiHS_AP1B1.
ExpressionAtlasiQ10567. baseline and differential.
GenevisibleiQ10567. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1150. 1 hit.
3.30.310.10. 1 hit.
InterProiIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00185. ARM. 2 hits.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAP1B1_HUMAN
AccessioniPrimary (citable) accession number: Q10567
Secondary accession number(s): C9JRD1
, F8WDL0, P78436, Q20WL3, Q86X54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 2, 2010
Last modified: November 30, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.