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Q10567 (AP1B1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit beta-1
Alternative name(s):
Adapter-related protein complex 1 subunit beta-1
Adaptor protein complex AP-1 subunit beta-1
Beta-1-adaptin
Beta-adaptin 1
Clathrin assembly protein complex 1 beta large chain
Golgi adaptor HA1/AP1 adaptin beta subunit
Gene names
Name:AP1B1
Synonyms:ADTB1, BAM22, CLAPB2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length949 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasmic vesicle
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class II

Traceable author statement. Source: Reactome

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

membrane organization

Traceable author statement. Source: Reactome

post-Golgi vesicle-mediated transport

Traceable author statement. Source: Reactome

regulation of defense response to virus by virus

Traceable author statement. Source: Reactome

viral process

Traceable author statement. Source: Reactome

   Cellular_componentGolgi membrane

Traceable author statement. Source: Reactome

clathrin adaptor complex

Inferred from electronic annotation. Source: InterPro

clathrin-coated vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

cytosol

Traceable author statement. Source: Reactome

lysosomal membrane

Traceable author statement. Source: Reactome

trans-Golgi network membrane

Traceable author statement. Source: Reactome

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 9733768. Source: UniProtKB

protein kinase binding

Inferred from sequence or structural similarity. Source: ParkinsonsUK-UCL

protein transporter activity

Inferred from electronic annotation. Source: InterPro

transporter activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ap1m1P355853EBI-1171303,EBI-1040251From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform A (identifier: Q10567-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q10567-2)

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.
Isoform C (identifier: Q10567-3)

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.
     923-925: Missing.
Isoform 4 (identifier: Q10567-4)

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.
     722-741: Missing.
     923-925: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 949948AP-1 complex subunit beta-1
PRO_0000193738

Regions

Compositional bias576 – 725150Pro-rich (stalk region)

Amino acid modifications

Modified residue21N-acetylthreonine By similarity
Modified residue2651N6-acetyllysine By similarity
Modified residue3181N6-acetyllysine Ref.6

Natural variations

Alternative sequence667 – 6737Missing in isoform B, isoform C and isoform 4.
VSP_000163
Alternative sequence722 – 74120Missing in isoform 4.
VSP_044928
Alternative sequence923 – 9253Missing in isoform C and isoform 4.
VSP_038753
Natural variant7771T → A. Ref.1 Ref.2 Ref.4 Ref.5
Corresponds to variant rs2857465 [ dbSNP | Ensembl ].
VAR_062816

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified March 2, 2010. Version 2.
Checksum: FE1BA762F9318585

FASTA949104,637
        10         20         30         40         50         60 
MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL FPDVVNCMQT 

        70         80         90        100        110        120 
DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP LIRALAVRTM GCIRVDKITE 

       130        140        150        160        170        180 
YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD INAQLVEDQG FLDTLKDLIS DSNPMVVANA 

       190        200        210        220        230        240 
VAALSEIAES HPSSNLLDLN PQSINKLLTA LNECTEWGQI FILDCLANYM PKDDREAQSI 

       250        260        270        280        290        300 
CERVTPRLSH ANSAVVLSAV KVLMKFMEML SKDLDYYGTL LKKLAPPLVT LLSAEPELQY 

       310        320        330        340        350        360 
VALRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN IAQVLAELKE 

       370        380        390        400        410        420 
YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ TKVNYVVQEA IVVIKDIFRK 

       430        440        450        460        470        480 
YPNKYESVIA TLCENLDSLD EPEARAAMIW IVGEYAERID NADELLESFL EGFHDESTQV 

       490        500        510        520        530        540 
QLQLLTAIVK LFLKKPTETQ ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV 

       550        560        570        580        590        600 
VLAEKPLISE ETDLIEPTLL DELICYIGTL ASVYHKPPSA FVEGGRGVVH KSLPPRTASS 

       610        620        630        640        650        660 
ESAESPETAP TGAPPGEQPD VIPAQGDLLG DLLNLDLGPP VSGPPLATSS VQMGAVDLLG 

       670        680        690        700        710        720 
GGLDSLMGDE PEGIGGTNFV APPTAAVPAN LGAPIGSGLS DLFDLTSGVG TLSGSYVAPK 

       730        740        750        760        770        780 
AVWLPAMKAK GLEISGTFTR QVGSISMDLQ LTNKALQVMT DFAIQFNRNS FGLAPATPLQ 

       790        800        810        820        830        840 
VHAPLSPNQT VEISLPLSTV GSVMKMEPLN NLQVAVKNNI DVFYFSTLYP LHILFVEDGK 

       850        860        870        880        890        900 
MDRQMFLATW KDIPNENEAQ FQIRDCPLNA EAASSKLQSS NIFTVAKRNV EGQDMLYQSL 

       910        920        930        940 
KLTNGIWVLA ELRIQPGNPS CTDLELSLKC RAPEVSQHVY QAYETILKN 

« Hide

Isoform B [UniParc].

Checksum: 2DB97E5FBB283A38
Show »

FASTA942103,921
Isoform C [UniParc].

Checksum: D8817293C15BD8E4
Show »

FASTA939103,564
Isoform 4 [UniParc].

Checksum: AC046601DF0170D3
Show »

FASTA919101,348

References

« Hide 'large scale' references
[1]"Characterization of a new member of the human beta-adaptin gene family from chromosome 22q12, a candidate meningioma gene."
Peyrard M., Fransson I., Xie Y.-G., Han F.-Y., Ruttledge M.H., Swahn S., Collins J.E., Dunham I., Collins V.P., Dumanski J.P.
Hum. Mol. Genet. 3:1393-1399(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), VARIANT ALA-777.
Tissue: Brain.
[2]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C), VARIANT ALA-777.
[3]"The DNA sequence of human chromosome 22."
Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. expand/collapse author list , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), VARIANT ALA-777.
Tissue: Lymph.
[5]"Structure of the promoter and genomic organization of the human beta'-adaptin gene (BAM22) from chromosome 22q12."
Peyrard M., Pan H.-Q., Kedra D., Fransson I., Swahn S., Hartman K., Clifton S.W., Roe B.A., Dumanski J.P.
Genomics 36:112-117(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-922, VARIANT ALA-777.
[6]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-318, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L13939 mRNA. Translation: AAC98702.1.
CT841508 mRNA. Translation: CAJ86438.1.
AC000041 Genomic DNA. No translation available.
AC002059 Genomic DNA. No translation available.
BC046242 mRNA. Translation: AAH46242.1.
U36268 expand/collapse EMBL AC list , U36250, U36251, U36252, U36253, U36254, U36255, U36256, U36257, U36258, U36259, U36260, U36261, U36262, U36263, U36264, U36265, U36266, U36267, AF379038, AF379039 Genomic DNA. Translation: AAC50684.2.
L48038 Genomic DNA. No translation available.
CCDSCCDS13855.1. [Q10567-1]
CCDS13856.2. [Q10567-3]
CCDS54515.1. [Q10567-4]
PIRI54360.
RefSeqNP_001118.3. NM_001127.3.
NP_001159491.1. NM_001166019.1.
NP_663782.2. NM_145730.2.
UniGeneHs.368794.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HMYX-ray7.00B1-584[»]
4P6ZX-ray3.00B1-584[»]
ProteinModelPortalQ10567.
SMRQ10567. Positions 12-574, 714-949.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106671. 38 interactions.
DIPDIP-24207N.
IntActQ10567. 26 interactions.
MINTMINT-1540570.
STRING9606.ENSP00000350199.

PTM databases

PhosphoSiteQ10567.

Polymorphism databases

DMDM290457628.

Proteomic databases

MaxQBQ10567.
PaxDbQ10567.
PRIDEQ10567.

Protocols and materials databases

DNASU162.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000317368; ENSP00000319361; ENSG00000100280. [Q10567-4]
ENST00000356015; ENSP00000348297; ENSG00000100280. [Q10567-2]
ENST00000357586; ENSP00000350199; ENSG00000100280. [Q10567-1]
ENST00000402502; ENSP00000386071; ENSG00000100280. [Q10567-3]
ENST00000405198; ENSP00000384194; ENSG00000100280. [Q10567-1]
ENST00000415447; ENSP00000387612; ENSG00000100280. [Q10567-3]
ENST00000432560; ENSP00000400065; ENSG00000100280. [Q10567-3]
GeneID162.
KEGGhsa:162.
UCSCuc003afi.3. human. [Q10567-3]
uc003afj.3. human. [Q10567-1]

Organism-specific databases

CTD162.
GeneCardsGC22M029723.
HGNCHGNC:554. AP1B1.
MIM600157. gene.
neXtProtNX_Q10567.
PharmGKBPA24844.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5096.
HOGENOMHOG000163270.
HOVERGENHBG050515.
InParanoidQ10567.
KOK12392.
OMAQPGNPSC.
OrthoDBEOG7BGHK0.
PhylomeDBQ10567.
TreeFamTF300318.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressQ10567.
BgeeQ10567.
CleanExHS_AP1B1.
GenevestigatorQ10567.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
2.60.40.1150. 1 hit.
3.30.310.10. 1 hit.
InterProIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERPTHR11134. PTHR11134. 1 hit.
PfamPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFPIRSF002291. AP_complex_beta. 1 hit.
SMARTSM00809. Alpha_adaptinC2. 1 hit.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.
ProtoNetSearch...

Other

ChiTaRSAP1B1. human.
GeneWikiAP1B1.
GenomeRNAi162.
NextBio35514080.
PROQ10567.
SOURCESearch...

Entry information

Entry nameAP1B1_HUMAN
AccessionPrimary (citable) accession number: Q10567
Secondary accession number(s): C9JRD1 expand/collapse secondary AC list , F8WDL0, P78436, Q20WL3, Q86X54
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 2, 2010
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM