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Q10504 (ODP1_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component

Short name=PDH E1 component
EC=1.2.4.1
Gene names
Name:aceE
Ordered Locus Names:Rv2241, MT2301
ORF Names:MTCY427.22
OrganismMycobacterium tuberculosis [Reference proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length901 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. AceE has reductase activity with pyruvate but does not react with 2-oxoglutarate. Ref.3

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactor

Magnesium. Ref.3

Thiamine pyrophosphate. Ref.3

Subunit structure

Homodimer By similarity. Makes part of the PDH complex, consisting of multiple copies of AceE (E1), DlaT (E2) and Lpd (E3). Ref.3

Biophysicochemical properties

Kinetic parameters:

KM=47 µM for pyruvate Ref.3

pH dependence:

Optimum pH is 8.0 for PDH complex activity. Half-maximal activity is observed at pH 7.0 and pH 9.0. Activity is abolished at pH < 5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 901901Pyruvate dehydrogenase E1 component
PRO_0000162246

Amino acid modifications

Cross-link346Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

Sequence LengthMass (Da)Tools
Q10504 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: F7910AA5722539BF

FASTA901100,217
        10         20         30         40         50         60 
MASYLPDIDP EETSEWLESF DTLLQRCGPS RARYLMLRLL ERAGEQRVAI PALTSTDYVN 

        70         80         90        100        110        120 
TIPTELEPWF PGDEDVERRY RAWIRWNAAI MVHRAQRPGV GVGGHISTYA SSAALYEVGF 

       130        140        150        160        170        180 
NHFFRGKSHP GGGDQVFIQG HASPGIYARA FLEGRLTAEQ LDGFRQEHSH VGGGLPSYPH 

       190        200        210        220        230        240 
PRLMPDFWEF PTVSMGLGPL NAIYQARFNH YLHDRGIKDT SDQHVWCFLG DGEMDEPESR 

       250        260        270        280        290        300 
GLAHVGALEG LDNLTFVINC NLQRLDGPVR GNGKIIQELE SFFRGAGWNV IKVVWGREWD 

       310        320        330        340        350        360 
ALLHADRDGA LVNLMNTTPD GDYQTYKAND GGYVRDHFFG RDPRTKALVE NMSDQDIWNL 

       370        380        390        400        410        420 
KRGGHDYRKV YAAYRAAVDH KGQPTVILAK TIKGYALGKH FEGRNATHQM KKLTLEDLKE 

       430        440        450        460        470        480 
FRDTQRIPVS DAQLEENPYL PPYYHPGLNA PEIRYMLDRR RALGGFVPER RTKSKALTLP 

       490        500        510        520        530        540 
GRDIYAPLKK GSGHQEVATT MATVRTFKEV LRDKQIGPRI VPIIPDEART FGMDSWFPSL 

       550        560        570        580        590        600 
KIYNRNGQLY TAVDADLMLA YKESEVGQIL HEGINEAGSV GSFIAAGTSY ATHNEPMIPI 

       610        620        630        640        650        660 
YIFYSMFGFQ RTGDSFWAAA DQMARGFVLG ATAGRTTLTG EGLQHADGHS LLLAATNPAV 

       670        680        690        700        710        720 
VAYDPAFAYE IAYIVESGLA RMCGENPENI FFYITVYNEP YVQPPEPENF DPEGVLRGIY 

       730        740        750        760        770        780 
RYHAATEQRT NKAQILASGV AMPAALRAAQ MLAAEWDVAA DVWSVTSWGE LNRDGVAIET 

       790        800        810        820        830        840 
EKLRHPDRPA GVPYVTRALE NARGPVIAVS DWMRAVPEQI RPWVPGTYLT LGTDGFGFSD 

       850        860        870        880        890        900 
TRPAARRYFN TDAESQVVAV LEALAGDGEI DPSVPVAAAR QYRIDDVAAA PEQTTDPGPG 


A 

« Hide

References

« Hide 'large scale' references
[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"Mycobacterium tuberculosis appears to lack alpha-ketoglutarate dehydrogenase and encodes pyruvate dehydrogenase in widely separated genes."
Tian J., Bryk R., Shi S., Erdjument-Bromage H., Tempst P., Nathan C.
Mol. Microbiol. 57:859-868(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, IDENTIFICATION IN THE PDH COMPLEX.
Strain: ATCC 25618 / H37Rv.
[4]"Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis."
Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H.
PLoS ONE 5:E8589-E8589(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PUPYLATION AT LYS-346, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000516 Genomic DNA. Translation: AAK46585.1.
AL123456 Genomic DNA. Translation: CCP45021.1.
PIRE70778.
RefSeqNP_216757.3. NC_000962.3.
NP_336771.1. NC_002755.2.
YP_006515664.1. NC_018143.1.

3D structure databases

ProteinModelPortalQ10504.
SMRQ10504. Positions 56-885.
ModBaseSearch...

Protein-protein interaction databases

STRING83332.Rv2241.

Proteomic databases

PaxDbQ10504.
PRIDEQ10504.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK46585; AAK46585; MT2301.
GeneID13318934.
887246.
924124.
KEGGmtc:MT2301.
mtu:Rv2241.
PATRIC18126809. VBIMycTub22151_2515.

Organism-specific databases

TubercuListRv2241.

Phylogenomic databases

eggNOGCOG2609.
HOGENOMHOG000115215.
KOK00163.
OMAPEETGEW.
ProtClustDBPRK09405.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
InterProIPR004660. 2-oxoA_DH_E1.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR015941. Transketolase-like_C.
IPR005474. Transketolase_N.
[Graphical view]
PfamPF00456. Transketolase_N. 1 hit.
[Graphical view]
PIRSFPIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAMSSF52922. Transketo_C_like. 1 hit.
TIGRFAMsTIGR00759. aceE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameODP1_MYCTU
AccessionPrimary (citable) accession number: Q10504
Secondary accession number(s): L0TBX6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names