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Protein

Apoptosis-inducing factor 1

Gene

aif1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Putative FAD-dependent oxidoreductase involved in the resistance to cercosporin and other singlet oxygen-generating photosensitizers. Translocates from mitochondria to the nucleus under apoptotic conditions, where it degrades DNA and induces apoptosis (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi99Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi117Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi120Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-inducing factor 1 (EC:1.-.-.-)
Gene namesi
Name:aif1
ORF Names:SPAC26F1.14c, SPAC29A4.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC26F1.14c.
PomBaseiSPAC26F1.14c. aif1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001166251 – 611Apoptosis-inducing factor 1Add BLAST611

Proteomic databases

MaxQBiQ10499.
PRIDEiQ10499.

PTM databases

SwissPalmiQ10499.

Interactioni

Protein-protein interaction databases

MINTiMINT-4699755.

Structurei

3D structure databases

ProteinModelPortaliQ10499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 154RieskePROSITE-ProRule annotationAdd BLAST99

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi38 – 41Poly-Ser4

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000276711.
InParanoidiQ10499.
OMAiRISAECH.
OrthoDBiEOG092C1TNC.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRFFINQP RFIQFTFLRN KKDRILGLQL GKQPFRMSSS SGLKQQGLAQ
60 70 80 90 100
KKKFQLEFDP SSVSKNGTKT EAKVVGTEFG VLLVRARNTY FATAGKCSHY
110 120 130 140 150
GAPLAKGVVT SDGHIVCPWH GACFNAATGD VEDTPAIAAL RTFPVTEEGD
160 170 180 190 200
GSLWIEVEDK NDNGASVLQP EGCWRNKATE VYNKGSVETE VTAPHVCIIG
210 220 230 240 250
GGKGASVAAE YLREKNFKGK ITIFTREDEV PYDRPKLSKS LLHDISKLAL
260 270 280 290 300
RSKEYYDDLD ISFHFNTDVT KIDLAEKKIY CGSDEKPTES YTKLILATGG
310 320 330 340 350
EPNKLPIPGL DSKNVYLLRS IADASKLAAV TTEAGDKKNI VIIGSSFIGL
360 370 380 390 400
ELAVVLKDHN VSVIGMESIP FEKVMGKEVG TALKALHEQN GIAFYLENSI
410 420 430 440 450
KEVKTSSNDS SKAEHIVLKD GQSIPADVVI LAAGVKPNLR YLGNAVSLEK
460 470 480 490 500
DGGVKVDEHC RVLGAEDVYA VGDIAHAPFA GLPSSGEKSH TRIEHWDVAG
510 520 530 540 550
NLGRVAADHI LFGNKAGYTT KSFTPYFWSA QGKQLRYCGN NAAEGFDDVV
560 570 580 590 600
IQGSLSDYKF ACFFTKGEKV VGVCSIMKDP VVSQCARLFI KDAMPSKSQL
610
KENFDVLSIP L
Length:611
Mass (Da):66,566
Last modified:May 16, 2012 - v3
Checksum:i3885A888830EDC76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB55362.4.
PIRiT38406.
RefSeqiXP_001713117.2. XM_001713065.2.

Genome annotation databases

EnsemblFungiiSPAC26F1.14c.1; SPAC26F1.14c.1:pep; SPAC26F1.14c.
GeneIDi3361554.
KEGGispo:SPAC26F1.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB55362.4.
PIRiT38406.
RefSeqiXP_001713117.2. XM_001713065.2.

3D structure databases

ProteinModelPortaliQ10499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4699755.

PTM databases

SwissPalmiQ10499.

Proteomic databases

MaxQBiQ10499.
PRIDEiQ10499.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC26F1.14c.1; SPAC26F1.14c.1:pep; SPAC26F1.14c.
GeneIDi3361554.
KEGGispo:SPAC26F1.14c.

Organism-specific databases

EuPathDBiFungiDB:SPAC26F1.14c.
PomBaseiSPAC26F1.14c. aif1.

Phylogenomic databases

HOGENOMiHOG000276711.
InParanoidiQ10499.
OMAiRISAECH.
OrthoDBiEOG092C1TNC.

Miscellaneous databases

PROiQ10499.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIF1_SCHPO
AccessioniPrimary (citable) accession number: Q10499
Secondary accession number(s): O14004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 16, 2012
Last modified: November 30, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1560 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.