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Q10490 (SYLC_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative leucine--tRNA ligase, cytoplasmic

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:lrs1
ORF Names:SPAC26F1.13c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length1111 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Cytoplasm Ref.3.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11111111Putative leucine--tRNA ligase, cytoplasmic
PRO_0000152154

Regions

Motif74 – 8411"HIGH" region
Motif737 – 7415"KMSKS" region

Sites

Binding site7401ATP By similarity

Amino acid modifications

Modified residue4601Phosphoserine Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q10490 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 88D654634F0415A9

FASTA1,111126,452
        10         20         30         40         50         60 
MATTEPSVEQ LETKTAKLKL ENTTKRDTLI ELEKKYQQKW QEEKAFEVDA PLEDVPIDEL 

        70         80         90        100        110        120 
RKKYPKFFGN MPYPYMNGAL HLGHAFTLSK VEFTTAFERL NGKRVLFPMG FHCTGMPICA 

       130        140        150        160        170        180 
SADRLSREIE MFGPSFDVPE EKEEEVEVEV KTPNAREDVT KHSGKKSKAA AKTAAVKYQF 

       190        200        210        220        230        240 
QIMESLGVPR TEIHKFADAK YWLSYFPPLC QRDCTEFGLG IDWRRSFITT DVNPYYDSFV 

       250        260        270        280        290        300 
RWQVNHLHDS GKIKFGERYT VYSIKDGQPC MDHDRKSGEG VGPQEYTGIK MEVLEFPEAA 

       310        320        330        340        350        360 
RKALQSIDLS NKKVCMIAAT LRPETMYGQT NCYVGPNITY GIYESNVPNE LFICTRRAAN 

       370        380        390        400        410        420 
NMAYQKLSKE RGVVSELGTI KGQDLIGALV NAPLSVHKQV YVLPMETVLA TKGTGVVTSV 

       430        440        450        460        470        480 
PSDSPDDFAT LTELRKKAEF YHLNPEWMKY EAVPIIRTPS YGDMCAEFLC KKLKIQSPKD 

       490        500        510        520        530        540 
VKQLAQAKEL AYKECFYQGT MIIGKYSGEK VETAKPKVRK ELIDQGLAFV YNEPEGQVIS 

       550        560        570        580        590        600 
RSGDDCIVAL CDQWFLDYGE ASWKAVTEKA LDRLNTFSPE VRNGFLKTLD WLSQWACARS 

       610        620        630        640        650        660 
YGLGTRLPWD PQFLVESLTD STIYMAYYTI CHLLHSDVYG KVPGALNIKP EQMTPEVWDH 

       670        680        690        700        710        720 
VFRQAPKPKN TSISDEALAR LCREFQYFYP FDIRASGKDL VPNHLTFCLY THTAIFDEKY 

       730        740        750        760        770        780 
WPKGIRANGH LLMNGEKMSK STGNFMTLHE ATKKFGADAT RLALADAGDT VDDANFEEAL 

       790        800        810        820        830        840 
ANSAILRLYT QEAWCKEMME NLDNLRTGPY NFHDKVFENE INQLIESSRE AFSATLFKAA 

       850        860        870        880        890        900 
LKSCFYDLQN ARDWYREVTA DRKMHRDLVC RWIETQVLLL ATFAPHWSEH IWLTTLKKPQ 

       910        920        930        940        950        960 
SIHVSGRFPQ VSSPVNTALS NSLLYIRTLS RVIREAEAAQ LKRQKKGKGM LFDPSKPKRL 

       970        980        990       1000       1010       1020 
TVFVAEKFPE WQAQYVALLQ KYYNESENKF DDKAIISSVD KKEMKRAMPF IQQFKQSVIN 

      1030       1040       1050       1060       1070       1080 
RGEHVSANSI FSRELGFNEL EVLREVKPYL VRNVGIQELR IVLLQKPADK SSAAIGLVES 

      1090       1100       1110 
GSDAGATVEI APNFANTVPG QPTFLFENVS A 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 739-1110.
Strain: PR745.
[3]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329670 Genomic DNA. Translation: CAA97370.1.
D89170 mRNA. Translation: BAA13832.1.
PIRT38407.
T42535.
RefSeqNP_594882.1. NM_001020311.2.

3D structure databases

ProteinModelPortalQ10490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid279123. 3 interactions.
MINTMINT-4699691.
STRING4896.SPAC26F1.13c-1.

Proteomic databases

PaxDbQ10490.
PRIDEQ10490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC26F1.13c.1; SPAC26F1.13c.1:pep; SPAC26F1.13c.
GeneID2542670.
KEGGspo:SPAC26F1.13c.

Organism-specific databases

PomBaseSPAC26F1.13c.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216621.
KOK01869.
OMAGFYEGVM.
OrthoDBEOG7TTQH2.
PhylomeDBQ10490.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20803718.
PROQ10490.

Entry information

Entry nameSYLC_SCHPO
AccessionPrimary (citable) accession number: Q10490
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries