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Q10490

- SYLC_SCHPO

UniProt

Q10490 - SYLC_SCHPO

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Protein
Putative leucine--tRNA ligase, cytoplasmic
Gene
lrs1, SPAC26F1.13c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei740 – 7401ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: PomBase

GO - Biological processi

  1. cytoplasmic translation Source: PomBase
  2. leucyl-tRNA aminoacylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:lrs1
ORF Names:SPAC26F1.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC26F1.13c.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11111111Putative leucine--tRNA ligase, cytoplasmic
PRO_0000152154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei460 – 4601Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10490.
PaxDbiQ10490.
PRIDEiQ10490.

Interactioni

Protein-protein interaction databases

BioGridi279123. 3 interactions.
MINTiMINT-4699691.
STRINGi4896.SPAC26F1.13c-1.

Structurei

3D structure databases

ProteinModelPortaliQ10490.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi74 – 8411"HIGH" region
Add
BLAST
Motifi737 – 7415"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216621.
KOiK01869.
OMAiYEGVMLV.
OrthoDBiEOG7TTQH2.
PhylomeDBiQ10490.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10490-1 [UniParc]FASTAAdd to Basket

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MATTEPSVEQ LETKTAKLKL ENTTKRDTLI ELEKKYQQKW QEEKAFEVDA     50
PLEDVPIDEL RKKYPKFFGN MPYPYMNGAL HLGHAFTLSK VEFTTAFERL 100
NGKRVLFPMG FHCTGMPICA SADRLSREIE MFGPSFDVPE EKEEEVEVEV 150
KTPNAREDVT KHSGKKSKAA AKTAAVKYQF QIMESLGVPR TEIHKFADAK 200
YWLSYFPPLC QRDCTEFGLG IDWRRSFITT DVNPYYDSFV RWQVNHLHDS 250
GKIKFGERYT VYSIKDGQPC MDHDRKSGEG VGPQEYTGIK MEVLEFPEAA 300
RKALQSIDLS NKKVCMIAAT LRPETMYGQT NCYVGPNITY GIYESNVPNE 350
LFICTRRAAN NMAYQKLSKE RGVVSELGTI KGQDLIGALV NAPLSVHKQV 400
YVLPMETVLA TKGTGVVTSV PSDSPDDFAT LTELRKKAEF YHLNPEWMKY 450
EAVPIIRTPS YGDMCAEFLC KKLKIQSPKD VKQLAQAKEL AYKECFYQGT 500
MIIGKYSGEK VETAKPKVRK ELIDQGLAFV YNEPEGQVIS RSGDDCIVAL 550
CDQWFLDYGE ASWKAVTEKA LDRLNTFSPE VRNGFLKTLD WLSQWACARS 600
YGLGTRLPWD PQFLVESLTD STIYMAYYTI CHLLHSDVYG KVPGALNIKP 650
EQMTPEVWDH VFRQAPKPKN TSISDEALAR LCREFQYFYP FDIRASGKDL 700
VPNHLTFCLY THTAIFDEKY WPKGIRANGH LLMNGEKMSK STGNFMTLHE 750
ATKKFGADAT RLALADAGDT VDDANFEEAL ANSAILRLYT QEAWCKEMME 800
NLDNLRTGPY NFHDKVFENE INQLIESSRE AFSATLFKAA LKSCFYDLQN 850
ARDWYREVTA DRKMHRDLVC RWIETQVLLL ATFAPHWSEH IWLTTLKKPQ 900
SIHVSGRFPQ VSSPVNTALS NSLLYIRTLS RVIREAEAAQ LKRQKKGKGM 950
LFDPSKPKRL TVFVAEKFPE WQAQYVALLQ KYYNESENKF DDKAIISSVD 1000
KKEMKRAMPF IQQFKQSVIN RGEHVSANSI FSRELGFNEL EVLREVKPYL 1050
VRNVGIQELR IVLLQKPADK SSAAIGLVES GSDAGATVEI APNFANTVPG 1100
QPTFLFENVS A 1111
Length:1,111
Mass (Da):126,452
Last modified:October 1, 1996 - v1
Checksum:i88D654634F0415A9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA97370.1.
D89170 mRNA. Translation: BAA13832.1.
PIRiT38407.
T42535.
RefSeqiNP_594882.1. NM_001020311.2.

Genome annotation databases

EnsemblFungiiSPAC26F1.13c.1; SPAC26F1.13c.1:pep; SPAC26F1.13c.
GeneIDi2542670.
KEGGispo:SPAC26F1.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA97370.1 .
D89170 mRNA. Translation: BAA13832.1 .
PIRi T38407.
T42535.
RefSeqi NP_594882.1. NM_001020311.2.

3D structure databases

ProteinModelPortali Q10490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279123. 3 interactions.
MINTi MINT-4699691.
STRINGi 4896.SPAC26F1.13c-1.

Proteomic databases

MaxQBi Q10490.
PaxDbi Q10490.
PRIDEi Q10490.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC26F1.13c.1 ; SPAC26F1.13c.1:pep ; SPAC26F1.13c .
GeneIDi 2542670.
KEGGi spo:SPAC26F1.13c.

Organism-specific databases

PomBasei SPAC26F1.13c.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216621.
KOi K01869.
OMAi YEGVMLV.
OrthoDBi EOG7TTQH2.
PhylomeDBi Q10490.

Miscellaneous databases

NextBioi 20803718.
PROi Q10490.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 739-1110.
    Strain: PR745.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSYLC_SCHPO
AccessioniPrimary (citable) accession number: Q10490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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