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Q10490

- SYLC_SCHPO

UniProt

Q10490 - SYLC_SCHPO

Protein

Putative leucine--tRNA ligase, cytoplasmic

Gene

lrs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei740 – 7401ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: PomBase

    GO - Biological processi

    1. cytoplasmic translation Source: PomBase
    2. leucyl-tRNA aminoacylation Source: PomBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:lrs1
    ORF Names:SPAC26F1.13c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC26F1.13c.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11111111Putative leucine--tRNA ligase, cytoplasmicPRO_0000152154Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei460 – 4601Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ10490.
    PaxDbiQ10490.
    PRIDEiQ10490.

    Interactioni

    Protein-protein interaction databases

    BioGridi279123. 3 interactions.
    MINTiMINT-4699691.
    STRINGi4896.SPAC26F1.13c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi74 – 8411"HIGH" regionAdd
    BLAST
    Motifi737 – 7415"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000216621.
    KOiK01869.
    OMAiYEGVMLV.
    OrthoDBiEOG7TTQH2.
    PhylomeDBiQ10490.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q10490-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTEPSVEQ LETKTAKLKL ENTTKRDTLI ELEKKYQQKW QEEKAFEVDA     50
    PLEDVPIDEL RKKYPKFFGN MPYPYMNGAL HLGHAFTLSK VEFTTAFERL 100
    NGKRVLFPMG FHCTGMPICA SADRLSREIE MFGPSFDVPE EKEEEVEVEV 150
    KTPNAREDVT KHSGKKSKAA AKTAAVKYQF QIMESLGVPR TEIHKFADAK 200
    YWLSYFPPLC QRDCTEFGLG IDWRRSFITT DVNPYYDSFV RWQVNHLHDS 250
    GKIKFGERYT VYSIKDGQPC MDHDRKSGEG VGPQEYTGIK MEVLEFPEAA 300
    RKALQSIDLS NKKVCMIAAT LRPETMYGQT NCYVGPNITY GIYESNVPNE 350
    LFICTRRAAN NMAYQKLSKE RGVVSELGTI KGQDLIGALV NAPLSVHKQV 400
    YVLPMETVLA TKGTGVVTSV PSDSPDDFAT LTELRKKAEF YHLNPEWMKY 450
    EAVPIIRTPS YGDMCAEFLC KKLKIQSPKD VKQLAQAKEL AYKECFYQGT 500
    MIIGKYSGEK VETAKPKVRK ELIDQGLAFV YNEPEGQVIS RSGDDCIVAL 550
    CDQWFLDYGE ASWKAVTEKA LDRLNTFSPE VRNGFLKTLD WLSQWACARS 600
    YGLGTRLPWD PQFLVESLTD STIYMAYYTI CHLLHSDVYG KVPGALNIKP 650
    EQMTPEVWDH VFRQAPKPKN TSISDEALAR LCREFQYFYP FDIRASGKDL 700
    VPNHLTFCLY THTAIFDEKY WPKGIRANGH LLMNGEKMSK STGNFMTLHE 750
    ATKKFGADAT RLALADAGDT VDDANFEEAL ANSAILRLYT QEAWCKEMME 800
    NLDNLRTGPY NFHDKVFENE INQLIESSRE AFSATLFKAA LKSCFYDLQN 850
    ARDWYREVTA DRKMHRDLVC RWIETQVLLL ATFAPHWSEH IWLTTLKKPQ 900
    SIHVSGRFPQ VSSPVNTALS NSLLYIRTLS RVIREAEAAQ LKRQKKGKGM 950
    LFDPSKPKRL TVFVAEKFPE WQAQYVALLQ KYYNESENKF DDKAIISSVD 1000
    KKEMKRAMPF IQQFKQSVIN RGEHVSANSI FSRELGFNEL EVLREVKPYL 1050
    VRNVGIQELR IVLLQKPADK SSAAIGLVES GSDAGATVEI APNFANTVPG 1100
    QPTFLFENVS A 1111
    Length:1,111
    Mass (Da):126,452
    Last modified:October 1, 1996 - v1
    Checksum:i88D654634F0415A9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA97370.1.
    D89170 mRNA. Translation: BAA13832.1.
    PIRiT38407.
    T42535.
    RefSeqiNP_594882.1. NM_001020311.2.

    Genome annotation databases

    EnsemblFungiiSPAC26F1.13c.1; SPAC26F1.13c.1:pep; SPAC26F1.13c.
    GeneIDi2542670.
    KEGGispo:SPAC26F1.13c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA97370.1 .
    D89170 mRNA. Translation: BAA13832.1 .
    PIRi T38407.
    T42535.
    RefSeqi NP_594882.1. NM_001020311.2.

    3D structure databases

    ProteinModelPortali Q10490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279123. 3 interactions.
    MINTi MINT-4699691.
    STRINGi 4896.SPAC26F1.13c-1.

    Proteomic databases

    MaxQBi Q10490.
    PaxDbi Q10490.
    PRIDEi Q10490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC26F1.13c.1 ; SPAC26F1.13c.1:pep ; SPAC26F1.13c .
    GeneIDi 2542670.
    KEGGi spo:SPAC26F1.13c.

    Organism-specific databases

    PomBasei SPAC26F1.13c.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000216621.
    KOi K01869.
    OMAi YEGVMLV.
    OrthoDBi EOG7TTQH2.
    PhylomeDBi Q10490.

    Miscellaneous databases

    NextBioi 20803718.
    PROi Q10490.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 739-1110.
      Strain: PR745.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSYLC_SCHPO
    AccessioniPrimary (citable) accession number: Q10490
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3