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Q10490

- SYLC_SCHPO

UniProt

Q10490 - SYLC_SCHPO

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Protein

Putative leucine--tRNA ligase, cytoplasmic

Gene

lrs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei740 – 7401ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: PomBase

GO - Biological processi

  1. cytoplasmic translation Source: PomBase
  2. leucyl-tRNA aminoacylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:lrs1
ORF Names:SPAC26F1.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC26F1.13c.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11111111Putative leucine--tRNA ligase, cytoplasmicPRO_0000152154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei460 – 4601Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10490.
PaxDbiQ10490.
PRIDEiQ10490.

Interactioni

Protein-protein interaction databases

BioGridi279123. 2 interactions.
MINTiMINT-4699691.
STRINGi4896.SPAC26F1.13c-1.

Structurei

3D structure databases

ProteinModelPortaliQ10490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi74 – 8411"HIGH" regionAdd
BLAST
Motifi737 – 7415"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216621.
InParanoidiQ10490.
KOiK01869.
OMAiYEGVMLV.
OrthoDBiEOG7TTQH2.
PhylomeDBiQ10490.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10490-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATTEPSVEQ LETKTAKLKL ENTTKRDTLI ELEKKYQQKW QEEKAFEVDA
60 70 80 90 100
PLEDVPIDEL RKKYPKFFGN MPYPYMNGAL HLGHAFTLSK VEFTTAFERL
110 120 130 140 150
NGKRVLFPMG FHCTGMPICA SADRLSREIE MFGPSFDVPE EKEEEVEVEV
160 170 180 190 200
KTPNAREDVT KHSGKKSKAA AKTAAVKYQF QIMESLGVPR TEIHKFADAK
210 220 230 240 250
YWLSYFPPLC QRDCTEFGLG IDWRRSFITT DVNPYYDSFV RWQVNHLHDS
260 270 280 290 300
GKIKFGERYT VYSIKDGQPC MDHDRKSGEG VGPQEYTGIK MEVLEFPEAA
310 320 330 340 350
RKALQSIDLS NKKVCMIAAT LRPETMYGQT NCYVGPNITY GIYESNVPNE
360 370 380 390 400
LFICTRRAAN NMAYQKLSKE RGVVSELGTI KGQDLIGALV NAPLSVHKQV
410 420 430 440 450
YVLPMETVLA TKGTGVVTSV PSDSPDDFAT LTELRKKAEF YHLNPEWMKY
460 470 480 490 500
EAVPIIRTPS YGDMCAEFLC KKLKIQSPKD VKQLAQAKEL AYKECFYQGT
510 520 530 540 550
MIIGKYSGEK VETAKPKVRK ELIDQGLAFV YNEPEGQVIS RSGDDCIVAL
560 570 580 590 600
CDQWFLDYGE ASWKAVTEKA LDRLNTFSPE VRNGFLKTLD WLSQWACARS
610 620 630 640 650
YGLGTRLPWD PQFLVESLTD STIYMAYYTI CHLLHSDVYG KVPGALNIKP
660 670 680 690 700
EQMTPEVWDH VFRQAPKPKN TSISDEALAR LCREFQYFYP FDIRASGKDL
710 720 730 740 750
VPNHLTFCLY THTAIFDEKY WPKGIRANGH LLMNGEKMSK STGNFMTLHE
760 770 780 790 800
ATKKFGADAT RLALADAGDT VDDANFEEAL ANSAILRLYT QEAWCKEMME
810 820 830 840 850
NLDNLRTGPY NFHDKVFENE INQLIESSRE AFSATLFKAA LKSCFYDLQN
860 870 880 890 900
ARDWYREVTA DRKMHRDLVC RWIETQVLLL ATFAPHWSEH IWLTTLKKPQ
910 920 930 940 950
SIHVSGRFPQ VSSPVNTALS NSLLYIRTLS RVIREAEAAQ LKRQKKGKGM
960 970 980 990 1000
LFDPSKPKRL TVFVAEKFPE WQAQYVALLQ KYYNESENKF DDKAIISSVD
1010 1020 1030 1040 1050
KKEMKRAMPF IQQFKQSVIN RGEHVSANSI FSRELGFNEL EVLREVKPYL
1060 1070 1080 1090 1100
VRNVGIQELR IVLLQKPADK SSAAIGLVES GSDAGATVEI APNFANTVPG
1110
QPTFLFENVS A
Length:1,111
Mass (Da):126,452
Last modified:October 1, 1996 - v1
Checksum:i88D654634F0415A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA97370.1.
D89170 mRNA. Translation: BAA13832.1.
PIRiT38407.
T42535.
RefSeqiNP_594882.1. NM_001020311.2.

Genome annotation databases

EnsemblFungiiSPAC26F1.13c.1; SPAC26F1.13c.1:pep; SPAC26F1.13c.
GeneIDi2542670.
KEGGispo:SPAC26F1.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA97370.1 .
D89170 mRNA. Translation: BAA13832.1 .
PIRi T38407.
T42535.
RefSeqi NP_594882.1. NM_001020311.2.

3D structure databases

ProteinModelPortali Q10490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279123. 2 interactions.
MINTi MINT-4699691.
STRINGi 4896.SPAC26F1.13c-1.

Proteomic databases

MaxQBi Q10490.
PaxDbi Q10490.
PRIDEi Q10490.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC26F1.13c.1 ; SPAC26F1.13c.1:pep ; SPAC26F1.13c .
GeneIDi 2542670.
KEGGi spo:SPAC26F1.13c.

Organism-specific databases

PomBasei SPAC26F1.13c.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216621.
InParanoidi Q10490.
KOi K01869.
OMAi YEGVMLV.
OrthoDBi EOG7TTQH2.
PhylomeDBi Q10490.

Miscellaneous databases

NextBioi 20803718.
PROi Q10490.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 739-1110.
    Strain: PR745.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSYLC_SCHPO
AccessioniPrimary (citable) accession number: Q10490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3