Reviewed, UniProtKB/Swiss-Prot Q10490 (SYLC_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative leucyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

ORF Names:

SPAC26F1.13c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1111 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW leucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1111	1111	Putative leucyl-tRNA synthetase, cytoplasmic		PRO_0000152154
Regions
	Motif	74 – 84	11	"HIGH" region
	Motif	737 – 741	5	"KMSKS" region
Sites
	Binding site	740	1	ATP By similarity
Amino acid modifications
	Modified residue	460	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q10490-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 88D654634F0415A9	FASTA	1,111	126,452
	10 20 30 40 50 60 MATTEPSVEQ LETKTAKLKL ENTTKRDTLI ELEKKYQQKW QEEKAFEVDA PLEDVPIDEL 70 80 90 100 110 120 RKKYPKFFGN MPYPYMNGAL HLGHAFTLSK VEFTTAFERL NGKRVLFPMG FHCTGMPICA 130 140 150 160 170 180 SADRLSREIE MFGPSFDVPE EKEEEVEVEV KTPNAREDVT KHSGKKSKAA AKTAAVKYQF 190 200 210 220 230 240 QIMESLGVPR TEIHKFADAK YWLSYFPPLC QRDCTEFGLG IDWRRSFITT DVNPYYDSFV 250 260 270 280 290 300 RWQVNHLHDS GKIKFGERYT VYSIKDGQPC MDHDRKSGEG VGPQEYTGIK MEVLEFPEAA 310 320 330 340 350 360 RKALQSIDLS NKKVCMIAAT LRPETMYGQT NCYVGPNITY GIYESNVPNE LFICTRRAAN 370 380 390 400 410 420 NMAYQKLSKE RGVVSELGTI KGQDLIGALV NAPLSVHKQV YVLPMETVLA TKGTGVVTSV 430 440 450 460 470 480 PSDSPDDFAT LTELRKKAEF YHLNPEWMKY EAVPIIRTPS YGDMCAEFLC KKLKIQSPKD 490 500 510 520 530 540 VKQLAQAKEL AYKECFYQGT MIIGKYSGEK VETAKPKVRK ELIDQGLAFV YNEPEGQVIS 550 560 570 580 590 600 RSGDDCIVAL CDQWFLDYGE ASWKAVTEKA LDRLNTFSPE VRNGFLKTLD WLSQWACARS 610 620 630 640 650 660 YGLGTRLPWD PQFLVESLTD STIYMAYYTI CHLLHSDVYG KVPGALNIKP EQMTPEVWDH 670 680 690 700 710 720 VFRQAPKPKN TSISDEALAR LCREFQYFYP FDIRASGKDL VPNHLTFCLY THTAIFDEKY 730 740 750 760 770 780 WPKGIRANGH LLMNGEKMSK STGNFMTLHE ATKKFGADAT RLALADAGDT VDDANFEEAL 790 800 810 820 830 840 ANSAILRLYT QEAWCKEMME NLDNLRTGPY NFHDKVFENE INQLIESSRE AFSATLFKAA 850 860 870 880 890 900 LKSCFYDLQN ARDWYREVTA DRKMHRDLVC RWIETQVLLL ATFAPHWSEH IWLTTLKKPQ 910 920 930 940 950 960 SIHVSGRFPQ VSSPVNTALS NSLLYIRTLS RVIREAEAAQ LKRQKKGKGM LFDPSKPKRL 970 980 990 1000 1010 1020 TVFVAEKFPE WQAQYVALLQ KYYNESENKF DDKAIISSVD KKEMKRAMPF IQQFKQSVIN 1030 1040 1050 1060 1070 1080 RGEHVSANSI FSRELGFNEL EVLREVKPYL VRNVGIQELR IVLLQKPADK SSAAIGLVES 1090 1100 1110 GSDAGATVEI APNFANTVPG QPTFLFENVS A

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 739-1110. Strain: PR745.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA97370.1. D89170 mRNA. Translation: BAA13832.1.
PIR	T38407. T42535.
RefSeq	NP_594882.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2542670.
KEGG	spo:SPAC26F1.13c.
NMPDR	fig|4896.1.peg.4852.
Organism-specific databases
GeneDB_Spombe	SPAC26F1.13c.
Phylogenomic databases
OMA	Q10490. HVTGTPI.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002950-MON.
BRENDA	6.1.1.4. 653.
Gene expression databases
ArrayExpress	Q10490.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR004493. Leu-tRNA-synth_Ia_arc/euk. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
TIGRFAMs	TIGR00395. leuS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYLC_SCHPO

Accession

Primary (citable) accession number: Q10490

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)