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Protein

Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

alg8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:alg8
ORF Names:SPAC17C9.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17C9.07.
PomBaseiSPAC17C9.07. alg8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3LumenalSequence analysis3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 59CytoplasmicSequence analysisAdd BLAST35
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 95LumenalSequence analysisAdd BLAST15
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 131CytoplasmicSequence analysisAdd BLAST15
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 156LumenalSequence analysis4
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Topological domaini178 – 180CytoplasmicSequence analysis3
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 315LumenalSequence analysisAdd BLAST114
Transmembranei316 – 336HelicalSequence analysisAdd BLAST21
Topological domaini337 – 341CytoplasmicSequence analysis5
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 389LumenalSequence analysisAdd BLAST27
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 412CytoplasmicSequence analysis2
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Topological domaini434 – 443LumenalSequence analysis10
Transmembranei444 – 464HelicalSequence analysisAdd BLAST21
Topological domaini465 – 473CytoplasmicSequence analysis9
Transmembranei474 – 494HelicalSequence analysisAdd BLAST21
Topological domaini495 – 501LumenalSequence analysis7

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741671 – 501Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST501

Proteomic databases

PRIDEiQ10479.

Interactioni

Protein-protein interaction databases

BioGridi278619. 20 interactors.
MINTiMINT-4699612.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000168174.
InParanoidiQ10479.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG092C28G5.
PhylomeDBiQ10479.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKLSMLYNA AIASTFVKVF LFPSYRSTDF EVHRNWLAIT HSLPISEWYK
60 70 80 90 100
SSISEWTLDY PPFFAYMECV LSWIAYFFGF DKAMLDPYNL NYVSPSTVVF
110 120 130 140 150
QRGSVIVLEL VLLFALREYV LSSNVKDQRN ALLTAIDIFL SPGLLIIDHI
160 170 180 190 200
HFQYNGFLFG LLLWSIVLAK PEKNMLLSAA IFSALICFKH IFLYVAPAYF
210 220 230 240 250
VYLLRVYCFT PNFRPQFLNI LKLGSTVISI FLLAFGPWIY MKQIPQLLSR
260 270 280 290 300
LFPFSRGLCH AYWAPNFWAL YSFVDRVAFA VLPRFGYALN QGTSINAPTR
310 320 330 340 350
GLVGESSFAV LPNIPPALTF YICLGLQITV LIKLFIKPTW RVFVGAVTLC
360 370 380 390 400
GWISFLFGWH VHEKAILMVI LPFSILSLID RRYLEAFRPL AVSGYLSLLP
410 420 430 440 450
LLFTLNEAPI KYLFTGAWIA MLLTFDKCAP VPVKRVFLLN RVNIAYISGF
460 470 480 490 500
VPLFIYNCFI HKLVMGDKFE FLPLMLLSTY AAWGIFWSFV SLLWLYFTDL

K
Length:501
Mass (Da):57,606
Last modified:October 1, 1996 - v1
Checksum:i4F9A08D85390CA94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA97353.1.
PIRiT11587.
RefSeqiNP_594599.1. NM_001020027.2.

Genome annotation databases

EnsemblFungiiSPAC17C9.07.1; SPAC17C9.07.1:pep; SPAC17C9.07.
GeneIDi2542143.
KEGGispo:SPAC17C9.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA97353.1.
PIRiT11587.
RefSeqiNP_594599.1. NM_001020027.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278619. 20 interactors.
MINTiMINT-4699612.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

PRIDEiQ10479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC17C9.07.1; SPAC17C9.07.1:pep; SPAC17C9.07.
GeneIDi2542143.
KEGGispo:SPAC17C9.07.

Organism-specific databases

EuPathDBiFungiDB:SPAC17C9.07.
PomBaseiSPAC17C9.07. alg8.

Phylogenomic databases

HOGENOMiHOG000168174.
InParanoidiQ10479.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG092C28G5.
PhylomeDBiQ10479.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ10479.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG8_SCHPO
AccessioniPrimary (citable) accession number: Q10479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.