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Protein

Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

alg8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:alg8
ORF Names:SPAC17C9.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17C9.07.
PomBaseiSPAC17C9.07. alg8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33LumenalSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 5935CytoplasmicSequence analysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence analysisAdd
BLAST
Topological domaini81 – 9515LumenalSequence analysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 13115CytoplasmicSequence analysisAdd
BLAST
Transmembranei132 – 15221HelicalSequence analysisAdd
BLAST
Topological domaini153 – 1564LumenalSequence analysis
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Topological domaini178 – 1803CytoplasmicSequence analysis
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Topological domaini202 – 315114LumenalSequence analysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence analysisAdd
BLAST
Topological domaini337 – 3415CytoplasmicSequence analysis
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 38927LumenalSequence analysisAdd
BLAST
Transmembranei390 – 41021HelicalSequence analysisAdd
BLAST
Topological domaini411 – 4122CytoplasmicSequence analysis
Transmembranei413 – 43321HelicalSequence analysisAdd
BLAST
Topological domaini434 – 44310LumenalSequence analysis
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Topological domaini465 – 4739CytoplasmicSequence analysis
Transmembranei474 – 49421HelicalSequence analysisAdd
BLAST
Topological domaini495 – 5017LumenalSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000174167Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi278619. 20 interactions.
MINTiMINT-4699612.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000168174.
InParanoidiQ10479.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG7DZ8VC.
PhylomeDBiQ10479.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKLSMLYNA AIASTFVKVF LFPSYRSTDF EVHRNWLAIT HSLPISEWYK
60 70 80 90 100
SSISEWTLDY PPFFAYMECV LSWIAYFFGF DKAMLDPYNL NYVSPSTVVF
110 120 130 140 150
QRGSVIVLEL VLLFALREYV LSSNVKDQRN ALLTAIDIFL SPGLLIIDHI
160 170 180 190 200
HFQYNGFLFG LLLWSIVLAK PEKNMLLSAA IFSALICFKH IFLYVAPAYF
210 220 230 240 250
VYLLRVYCFT PNFRPQFLNI LKLGSTVISI FLLAFGPWIY MKQIPQLLSR
260 270 280 290 300
LFPFSRGLCH AYWAPNFWAL YSFVDRVAFA VLPRFGYALN QGTSINAPTR
310 320 330 340 350
GLVGESSFAV LPNIPPALTF YICLGLQITV LIKLFIKPTW RVFVGAVTLC
360 370 380 390 400
GWISFLFGWH VHEKAILMVI LPFSILSLID RRYLEAFRPL AVSGYLSLLP
410 420 430 440 450
LLFTLNEAPI KYLFTGAWIA MLLTFDKCAP VPVKRVFLLN RVNIAYISGF
460 470 480 490 500
VPLFIYNCFI HKLVMGDKFE FLPLMLLSTY AAWGIFWSFV SLLWLYFTDL

K
Length:501
Mass (Da):57,606
Last modified:October 1, 1996 - v1
Checksum:i4F9A08D85390CA94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA97353.1.
PIRiT11587.
RefSeqiNP_594599.1. NM_001020027.2.

Genome annotation databases

EnsemblFungiiSPAC17C9.07.1; SPAC17C9.07.1:pep; SPAC17C9.07.
GeneIDi2542143.
KEGGispo:SPAC17C9.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA97353.1.
PIRiT11587.
RefSeqiNP_594599.1. NM_001020027.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278619. 20 interactions.
MINTiMINT-4699612.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC17C9.07.1; SPAC17C9.07.1:pep; SPAC17C9.07.
GeneIDi2542143.
KEGGispo:SPAC17C9.07.

Organism-specific databases

EuPathDBiFungiDB:SPAC17C9.07.
PomBaseiSPAC17C9.07. alg8.

Phylogenomic databases

HOGENOMiHOG000168174.
InParanoidiQ10479.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG7DZ8VC.
PhylomeDBiQ10479.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ10479.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALG8_SCHPO
AccessioniPrimary (citable) accession number: Q10479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.