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Q10475

- IF4G_SCHPO

UniProt

Q10475 - IF4G_SCHPO

Protein

Eukaryotic translation initiation factor 4 gamma

Gene

tif471

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.1 Publication

    GO - Molecular functioni

    1. protein binding Source: PomBase
    2. structural molecule activity Source: PomBase
    3. translation initiation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. cytoplasmic translational initiation Source: PomBase
    2. ribosomal large subunit biogenesis Source: PomBase

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 4 gamma
    Short name:
    eIF-4-gamma
    Short name:
    eIF-4G
    Gene namesi
    Name:tif471
    ORF Names:SPAC17C9.03
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC17C9.03.

    Subcellular locationi

    Cytoplasmperinuclear region 1 Publication
    Note: Localized to the perinuclear region, the growing tips and septum.

    GO - Cellular componenti

    1. cytoplasmic stress granule Source: PomBase
    2. cytosol Source: PomBase
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14031403Eukaryotic translation initiation factor 4 gammaPRO_0000213333Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei83 – 831Phosphoserine1 Publication
    Modified residuei452 – 4521Phosphoserine1 Publication
    Modified residuei455 – 4551Phosphoserine1 Publication
    Modified residuei456 – 4561Phosphoserine1 Publication
    Modified residuei459 – 4591Phosphoserine1 Publication
    Modified residuei866 – 8661Phosphoserine1 Publication
    Modified residuei882 – 8821Phosphoserine1 Publication
    Modified residuei884 – 8841Phosphothreonine1 Publication
    Modified residuei886 – 8861Phosphoserine1 Publication
    Modified residuei911 – 9111Phosphoserine1 Publication
    Modified residuei919 – 9191Phosphoserine1 Publication
    Modified residuei921 – 9211Phosphoserine1 Publication
    Modified residuei923 – 9231Phosphotyrosine1 Publication
    Modified residuei1333 – 13331Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ10475.
    PaxDbiQ10475.
    PRIDEiQ10475.

    Interactioni

    Protein-protein interaction databases

    BioGridi278758. 11 interactions.
    IntActiQ10475. 2 interactions.
    MINTiMINT-4699578.
    STRINGi4896.SPAC17C9.03-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1009 – 1245237MIF4GAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MIF4G domain.Curated

    Phylogenomic databases

    eggNOGiNOG301289.
    KOiK03260.
    OMAiSNTHSHA.
    OrthoDBiEOG7VTDWG.

    Family and domain databases

    Gene3Di1.25.40.180. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR022745. eIF4G1_eIF4E-bd.
    IPR012969. Fibrinogen_BP.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR003890. MIF4G-like_typ-3.
    [Graphical view]
    PfamiPF12152. eIF_4G1. 1 hit.
    PF08017. Fibrinogen_BP. 1 hit.
    PF02854. MIF4G. 1 hit.
    [Graphical view]
    SMARTiSM00543. MIF4G. 1 hit.
    [Graphical view]
    SUPFAMiSSF101489. SSF101489. 1 hit.
    SSF48371. SSF48371. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q10475-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKPPSNTP KFSYARALAS SQSNKSNSTK ASENNTATAE KQAVKPSGVE     50
    PTNTSRANAQ KKTESTGKIT SEADTEKYNS SKSPVNKEGS VEKKSSEKSS 100
    TNNKPWRGDN TSKPSANSSA ERTSSQHQKP ETSSQIGKDN AAPVENVNEK 150
    STSQETAPPV STVPIQFGSI TRNAAIPSKP KVSGNMQNKS GVSSYSSKSQ 200
    SVNSSVTSNP PHTEEPVAAK PEASSTATKG PRPTTSASNT NTSPANGAPT 250
    NKPSTDINTT DPATQTTQVS ASNSPALSGS STPSNTSSRS NRQNHGNFSE 300
    KRHYDRYGNS HPSYNKYSHY QHGFNYNNSG NNRNESGHPR FRNSRRNYNN 350
    QGAYPTYMSN GRSANQSPRN NPQNVNNGST PIQIPVSLQT PYGQVYGQPQ 400
    YIVDPNMVQY GPILQPGYVP QYYPVYHQTP YTQNFPNMSR SGSQVSDQVV 450
    ESPNSSTLSP RNGFAPIVKQ QKKSSALKIV NPVTHTEVVV PQKNASSPNP 500
    SETNSRAETP TAAPPQISEE EASQRKDAIK LAIQQRIQEK AEAEAKRKAE 550
    EKARLEAEEN AKREAEEQAK REAEEKAKRE AEEKAKREAE EKAKREAEEN 600
    AKREAEEKAK REAEEKAKRE AEEKAKREAE EKAKREAEEK AKREAEEKAK 650
    REAEEKAKRE AEENAKREAE EKAKREAEEN AKREAEEKVK RETEENAKRK 700
    AEEEGKREAD KNPEIKSSAP LASSEANVDT SKQTNATEPE VVDKTKVEKL 750
    KASEGKSTSS LSSPSHSTSS KRDLLSGLES LSLKTNPKSE QCLESLLNSQ 800
    FITDFSALVY PSTIKPPSTE EALKAGKYEY DVPFLLQFQS VYTDKPMKGW 850
    DERMKETVAS AFSDKSSRGM YSSSRQSSRS GSNTHSHAGP GFGGPSERKG 900
    ISRLGIDRGF SSSGAGFGSG SNYKSAPSRG VSHHGHGGMS GSHRGSQRGS 950
    RRGGGERDKP DPSSLTIPVD QVAPLQLSAN RWQPKKLTEK PAETKGEDEE 1000
    ALLPPEVVQR KVKGSLNKMT LEKFDKISDQ ILEIAMQSRK ENDGRTLKQV 1050
    IQLTFEKATD EPNFSNMYAR FARKMMDSID DSIRDEGVLD KNNQPVRGGL 1100
    LFRKYLLSRC QEDFERGWKA NLPSGKAGEA EIMSDEYYVA AAIKRRGLGL 1150
    VRFIGELFKL SMLSEKIMHE CIKRLLGNVT DPEEEEIESL CRLLMTVGVN 1200
    IDATEKGHAA MDVYVLRMET ITKIPNLPSR IKFMLMDVMD SRKNGWAVKN 1250
    EVEKGPKTIA EIHEEAERKK ALAESQRPSS GRMHGRDMNR GDSRMGGRGS 1300
    NPPFSSSDWS NNKDGYARLG QGIRGLKSGT QGSHGPTSLS SMLKGGSVSR 1350
    TPSRQNSALR REQSVRAPPS NVAVTSANSF ELLEEHDHDN DGGQKDSNSK 1400
    TSS 1403
    Length:1,403
    Mass (Da):154,035
    Last modified:October 1, 1996 - v1
    Checksum:i0317EE65BE2A1E63
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1017 – 10171N → T in BAA13842. (PubMed:9501991)Curated
    Sequence conflicti1249 – 12491K → L in BAA13842. (PubMed:9501991)Curated
    Sequence conflicti1253 – 12531E → G in BAA13842. (PubMed:9501991)Curated
    Sequence conflicti1257 – 12571K → G in BAA13842. (PubMed:9501991)Curated
    Sequence conflicti1301 – 13011N → T in BAA13842. (PubMed:9501991)Curated
    Sequence conflicti1304 – 13041F → P in BAA13842. (PubMed:9501991)Curated
    Sequence conflicti1353 – 13531S → Y in BAA13842. (PubMed:9501991)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA97349.1.
    D89180 mRNA. Translation: BAA13842.1.
    PIRiT11583.
    T42624.
    RefSeqiNP_594602.1. NM_001020030.2.

    Genome annotation databases

    EnsemblFungiiSPAC17C9.03.1; SPAC17C9.03.1:pep; SPAC17C9.03.
    GeneIDi2542290.
    KEGGispo:SPAC17C9.03.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA97349.1 .
    D89180 mRNA. Translation: BAA13842.1 .
    PIRi T11583.
    T42624.
    RefSeqi NP_594602.1. NM_001020030.2.

    3D structure databases

    ProteinModelPortali Q10475.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278758. 11 interactions.
    IntActi Q10475. 2 interactions.
    MINTi MINT-4699578.
    STRINGi 4896.SPAC17C9.03-1.

    Proteomic databases

    MaxQBi Q10475.
    PaxDbi Q10475.
    PRIDEi Q10475.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC17C9.03.1 ; SPAC17C9.03.1:pep ; SPAC17C9.03 .
    GeneIDi 2542290.
    KEGGi spo:SPAC17C9.03.

    Organism-specific databases

    PomBasei SPAC17C9.03.

    Phylogenomic databases

    eggNOGi NOG301289.
    KOi K03260.
    OMAi SNTHSHA.
    OrthoDBi EOG7VTDWG.

    Miscellaneous databases

    NextBioi 20803353.
    PROi Q10475.

    Family and domain databases

    Gene3Di 1.25.40.180. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR022745. eIF4G1_eIF4E-bd.
    IPR012969. Fibrinogen_BP.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR003890. MIF4G-like_typ-3.
    [Graphical view ]
    Pfami PF12152. eIF_4G1. 1 hit.
    PF08017. Fibrinogen_BP. 1 hit.
    PF02854. MIF4G. 1 hit.
    [Graphical view ]
    SMARTi SM00543. MIF4G. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101489. SSF101489. 1 hit.
    SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Overproduction of a conserved domain of fission yeast and mammalian translation initiation factor eIF4G causes aberrant cell morphology and results in disruption of the localization of F-actin and the organization of microtubules."
      Hashemzadeh-Bonehi L., Curtis P.S., Morley S.J., Thorpe J.R., Pain V.M.
      Genes Cells 8:163-178(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 865-1403.
      Strain: PR745.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83; SER-452; SER-455; SER-456; SER-459; SER-866; SER-882; THR-884; SER-886; SER-911; SER-919; SER-921; TYR-923 AND SER-1333, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiIF4G_SCHPO
    AccessioniPrimary (citable) accession number: Q10475
    Secondary accession number(s): P78832
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3