Eukaryotic translation initiation factor 4 gamma - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 4 gamma
Short name=eIF-4-gamma
Short name=eIF-4G

Gene names

Name:	tif471
ORF Names:	SPAC17C9.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length	1403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Ref.1

Subcellular location

Cytoplasm › perinuclear region. Note: Localized to the perinuclear region, the growing tips and septum. Ref.1

Sequence similarities

Belongs to the eukaryotic initiation factor 4G family.

Contains 1 MIF4G domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA metabolic process Inferred from electronic annotation. Source: InterPro cytoplasmic translational initiation Non-traceable author statement. Source: PomBase ribosomal large subunit biogenesis Inferred from sequence orthology. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro structural molecule activity Traceable author statement Ref.1. Source: PomBase translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1403

1403

Eukaryotic translation initiation factor 4 gamma

PRO_0000213333

Regions

Domain

1009 – 1245

237

MIF4G

Amino acid modifications

Modified residue

83

1

Phosphoserine Ref.4

Modified residue

452

1

Phosphoserine Ref.4

Modified residue

455

1

Phosphoserine Ref.4

Modified residue

456

1

Phosphoserine Ref.4

Modified residue

459

1

Phosphoserine Ref.4

Modified residue

866

1

Phosphoserine Ref.4

Modified residue

882

1

Phosphoserine Ref.4

Modified residue

884

1

Phosphothreonine Ref.4

Modified residue

886

1

Phosphoserine Ref.4

Modified residue

911

1

Phosphoserine Ref.4

Modified residue

919

1

Phosphoserine Ref.4

Modified residue

921

1

Phosphoserine Ref.4

Modified residue

923

1

Phosphotyrosine Ref.4

Modified residue

1333

1

Phosphoserine Ref.4

Experimental info

Sequence conflict

1017

1

N → T in BAA13842. Ref.3

Sequence conflict

1249

1

K → L in BAA13842. Ref.3

Sequence conflict

1253

1

E → G in BAA13842. Ref.3

Sequence conflict

1257

1

K → G in BAA13842. Ref.3

Sequence conflict

1301

1

N → T in BAA13842. Ref.3

Sequence conflict

1304

1

F → P in BAA13842. Ref.3

Sequence conflict

1353

1

S → Y in BAA13842. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q10475 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0317EE65BE2A1E63
Show »

FASTA

1,403

154,035

        10         20         30         40         50         60 
MSSKPPSNTP KFSYARALAS SQSNKSNSTK ASENNTATAE KQAVKPSGVE PTNTSRANAQ 

        70         80         90        100        110        120 
KKTESTGKIT SEADTEKYNS SKSPVNKEGS VEKKSSEKSS TNNKPWRGDN TSKPSANSSA 

       130        140        150        160        170        180 
ERTSSQHQKP ETSSQIGKDN AAPVENVNEK STSQETAPPV STVPIQFGSI TRNAAIPSKP 

       190        200        210        220        230        240 
KVSGNMQNKS GVSSYSSKSQ SVNSSVTSNP PHTEEPVAAK PEASSTATKG PRPTTSASNT 

       250        260        270        280        290        300 
NTSPANGAPT NKPSTDINTT DPATQTTQVS ASNSPALSGS STPSNTSSRS NRQNHGNFSE 

       310        320        330        340        350        360 
KRHYDRYGNS HPSYNKYSHY QHGFNYNNSG NNRNESGHPR FRNSRRNYNN QGAYPTYMSN 

       370        380        390        400        410        420 
GRSANQSPRN NPQNVNNGST PIQIPVSLQT PYGQVYGQPQ YIVDPNMVQY GPILQPGYVP 

       430        440        450        460        470        480 
QYYPVYHQTP YTQNFPNMSR SGSQVSDQVV ESPNSSTLSP RNGFAPIVKQ QKKSSALKIV 

       490        500        510        520        530        540 
NPVTHTEVVV PQKNASSPNP SETNSRAETP TAAPPQISEE EASQRKDAIK LAIQQRIQEK 

       550        560        570        580        590        600 
AEAEAKRKAE EKARLEAEEN AKREAEEQAK REAEEKAKRE AEEKAKREAE EKAKREAEEN 

       610        620        630        640        650        660 
AKREAEEKAK REAEEKAKRE AEEKAKREAE EKAKREAEEK AKREAEEKAK REAEEKAKRE 

       670        680        690        700        710        720 
AEENAKREAE EKAKREAEEN AKREAEEKVK RETEENAKRK AEEEGKREAD KNPEIKSSAP 

       730        740        750        760        770        780 
LASSEANVDT SKQTNATEPE VVDKTKVEKL KASEGKSTSS LSSPSHSTSS KRDLLSGLES 

       790        800        810        820        830        840 
LSLKTNPKSE QCLESLLNSQ FITDFSALVY PSTIKPPSTE EALKAGKYEY DVPFLLQFQS 

       850        860        870        880        890        900 
VYTDKPMKGW DERMKETVAS AFSDKSSRGM YSSSRQSSRS GSNTHSHAGP GFGGPSERKG 

       910        920        930        940        950        960 
ISRLGIDRGF SSSGAGFGSG SNYKSAPSRG VSHHGHGGMS GSHRGSQRGS RRGGGERDKP 

       970        980        990       1000       1010       1020 
DPSSLTIPVD QVAPLQLSAN RWQPKKLTEK PAETKGEDEE ALLPPEVVQR KVKGSLNKMT 

      1030       1040       1050       1060       1070       1080 
LEKFDKISDQ ILEIAMQSRK ENDGRTLKQV IQLTFEKATD EPNFSNMYAR FARKMMDSID 

      1090       1100       1110       1120       1130       1140 
DSIRDEGVLD KNNQPVRGGL LFRKYLLSRC QEDFERGWKA NLPSGKAGEA EIMSDEYYVA 

      1150       1160       1170       1180       1190       1200 
AAIKRRGLGL VRFIGELFKL SMLSEKIMHE CIKRLLGNVT DPEEEEIESL CRLLMTVGVN 

      1210       1220       1230       1240       1250       1260 
IDATEKGHAA MDVYVLRMET ITKIPNLPSR IKFMLMDVMD SRKNGWAVKN EVEKGPKTIA 

      1270       1280       1290       1300       1310       1320 
EIHEEAERKK ALAESQRPSS GRMHGRDMNR GDSRMGGRGS NPPFSSSDWS NNKDGYARLG 

      1330       1340       1350       1360       1370       1380 
QGIRGLKSGT QGSHGPTSLS SMLKGGSVSR TPSRQNSALR REQSVRAPPS NVAVTSANSF 

      1390       1400 
ELLEEHDHDN DGGQKDSNSK TSS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overproduction of a conserved domain of fission yeast and mammalian translation initiation factor eIF4G causes aberrant cell morphology and results in disruption of the localization of F-actin and the organization of microtubules." Hashemzadeh-Bonehi L., Curtis P.S., Morley S.J., Thorpe J.R., Pain V.M. Genes Cells 8:163-178(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 865-1403. Strain: PR745.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83; SER-452; SER-455; SER-456; SER-459; SER-866; SER-882; THR-884; SER-886; SER-911; SER-919; SER-921; TYR-923 AND SER-1333, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA97349.1. D89180 mRNA. Translation: BAA13842.1.
PIR	T11583. T42624.
RefSeq	NP_594602.1. NM_001020030.2.
3D structure databases
ProteinModelPortal	Q10475.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10475. 2 interactions.
STRING	4896.SPAC17C9.03-1.
Proteomic databases
PaxDb	Q10475.
PRIDE	Q10475.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC17C9.03.1; SPAC17C9.03.1:pep; SPAC17C9.03.
GeneID	2542290.
KEGG	spo:SPAC17C9.03.
Organism-specific databases
PomBase	SPAC17C9.03.
Phylogenomic databases
eggNOG	NOG301289.
KO	K03260.
OMA	GDSDYSR.
OrthoDB	EOG4CRQ7C.
Family and domain databases
Gene3D	1.25.40.180. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR022745. eIF4G1_eIF4E-bd. IPR012969. Fibrinogen_BP. IPR016021. MIF4-like_typ_1/2/3. IPR003890. MIF4G-like_typ-3. [Graphical view]
Pfam	PF12152. eIF_4G1. 1 hit. PF08017. Fibrinogen_BP. 1 hit. PF02854. MIF4G. 1 hit. [Graphical view]
SMART	SM00543. MIF4G. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF101489. eIF4G1_eIF4E-bd. 1 hit.
ProtoNet	Search...
Other
NextBio	20803353.

Entry information

Entry name

IF4G_SCHPO

Accession

Primary (citable) accession number: Q10475
Secondary accession number(s): P78832

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Translation initiation factors

List of translation initiation factor entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents