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Protein

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Gene

Mgat3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R = UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • N-acetylglucosamine metabolic process Source: MGI
  • protein N-linked glycosylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT17. Glycosyltransferase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (EC:2.4.1.144)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
Short name:
GNT-III
Short name:
GlcNAc-T III
Short name:
N-acetylglucosaminyltransferase III
Gene namesi
Name:Mgat3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104532. Mgat3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2316Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini24 – 538515LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferasePRO_0000188843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ10470.
PaxDbiQ10470.
PeptideAtlasiQ10470.
PRIDEiQ10470.

PTM databases

iPTMnetiQ10470.
PhosphoSiteiQ10470.

Expressioni

Tissue specificityi

Highly expressed in brain and kidney and to a much lesser extent in stomach, heart, intestine, uterus, testis, ovary and lung. Not present in spleen, liver and muscle.

Gene expression databases

CleanExiMM_MGAT3.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043077.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 8652Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 17 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEQJ. Eukaryota.
ENOG410YQKP. LUCA.
HOGENOMiHOG000113579.
HOVERGENiHBG052468.
InParanoidiQ10470.
PhylomeDBiQ10470.

Family and domain databases

InterProiIPR006813. Glyco_trans_17.
[Graphical view]
PANTHERiPTHR12224. PTHR12224. 2 hits.
PfamiPF04724. Glyco_transf_17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLISSFF
60 70 80 90 100
WNNAPVTPQA SPEPGDPDLL RTPLYSHSPL LQPLSPSKAT EELHRVDFVL
110 120 130 140 150
PEDTTEYFVR TKAGGVCFKP GTRMLEKPSP GRTEEKTEVS EGSSARGPAR
160 170 180 190 200
RPMRHVLSSR ERLGSRGTRR KWVECVCLPG WHGPSCGVPT VVQYSNLPTK
210 220 230 240 250
ERLVPREVPR RVINAININH EFDLLDVRFH ELGDVVDAFV VCDSNFTAYG
260 270 280 290 300
EPRPLKFREM LTNGTFEYIR HKVLYVFLDH FPPGGRQDGW IADDYLRTFL
310 320 330 340 350
TQDGVSRLRN LRPDDVFIID DADEIPARDG VLFLKLYDGW TEPFAFHMRK
360 370 380 390 400
SLYGFFWKQP GTLEVVSGCT MDMLQAVYGL DGIRLRRRQY YTMPNFRQYE
410 420 430 440 450
NRTGHILVQW SLGSPLHFAG WHCSWCFTPE GIYFKLVSAQ NGDFPRWGDY
460 470 480 490 500
EDKRDLNYIR SLIRTGGWFD GTQQEYPPAD PSEHMYAPKY LLKNYDQFRY
510 520 530
LLENPYREPK STVEGGRQNQ GSDGRSSAVR GKLDTAEG
Length:538
Mass (Da):62,003
Last modified:March 15, 2005 - v2
Checksum:iE5B7CF71C2022994
GO

Sequence cautioni

The sequence AAB71422.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti46 – 461I → V in strain: 129/SvJ.
Natural varianti66 – 661D → G in strain: 129/SvJ.
Natural varianti137 – 1371T → P in strain: 129/SvJ.
Natural varianti159 – 1591S → T in strain: 129/SvJ.
Natural varianti243 – 2431D → E in strain: 129/SvJ.
Natural varianti526 – 5261S → P in strain: 129/SvJ.
Natural varianti536 – 5361A → V in strain: 129/SvJ.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39373 Genomic DNA. Translation: AAB71422.1. Different initiation.
U66844 Genomic DNA. Translation: AAC53064.1.
CCDSiCCDS27660.1.
PIRiJC4362.
UniGeneiMm.299693.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

beta-1,4-mannosyl-glycoprotein beta 1,4-GlcNAc T III (Mgat3)

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39373 Genomic DNA. Translation: AAB71422.1. Different initiation.
U66844 Genomic DNA. Translation: AAC53064.1.
CCDSiCCDS27660.1.
PIRiJC4362.
UniGeneiMm.299693.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043077.

Protein family/group databases

CAZyiGT17. Glycosyltransferase Family 17.

PTM databases

iPTMnetiQ10470.
PhosphoSiteiQ10470.

Proteomic databases

MaxQBiQ10470.
PaxDbiQ10470.
PeptideAtlasiQ10470.
PRIDEiQ10470.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:104532. Mgat3.

Phylogenomic databases

eggNOGiENOG410IEQJ. Eukaryota.
ENOG410YQKP. LUCA.
HOGENOMiHOG000113579.
HOVERGENiHBG052468.
InParanoidiQ10470.
PhylomeDBiQ10470.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ10470.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MGAT3.

Family and domain databases

InterProiIPR006813. Glyco_trans_17.
[Graphical view]
PANTHERiPTHR12224. PTHR12224. 2 hits.
PfamiPF04724. Glyco_transf_17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and chromosomal mapping of the mouse Mgat3 gene encoding N-acetylglucosaminyltransferase III."
    Bhaumik M., Seldin M.F., Stanley P.
    Gene 164:295-300(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Liver.
  2. "Isolation, characterization and inactivation of the mouse Mgat3 gene: the bisecting N-acetylglucosamine in asparagine-linked oligosaccharides appears dispensable for viability and reproduction."
    Priatel J.J., Sarkar M., Schachter H., Marth J.D.
    Glycobiology 7:45-56(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Liver.

Entry informationi

Entry nameiMGAT3_MOUSE
AccessioniPrimary (citable) accession number: Q10470
Secondary accession number(s): P70386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 15, 2005
Last modified: July 6, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.