Q10470 (MGAT3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
EC=2.4.1.144

Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
Short name=GNT-III
Short name=GlcNAc-T III
Short name=N-acetylglucosaminyltransferase III

Gene names

Name:

Mgat3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R = UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Highly expressed in brain and kidney and to a much lesser extent in stomach, heart, intestine, uterus, testis, ovary and lung. Not present in spleen, liver and muscle.
Sequence similarities	Belongs to the glycosyltransferase 17 family.
Sequence caution	The sequence AAB71422.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase		PRO_0000188843
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 23	16	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 538	515	Lumenal Potential
	Compositional bias	35 – 86	52	Pro-rich
Amino acid modifications
	Modified residue	265	1	Phosphothreonine Ref.3
	Glycosylation	245	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	46	1	I → V in strain: 129/SvJ.
	Natural variant	66	1	D → G in strain: 129/SvJ.
	Natural variant	137	1	T → P in strain: 129/SvJ.
	Natural variant	159	1	S → T in strain: 129/SvJ.
	Natural variant	243	1	D → E in strain: 129/SvJ.
	Natural variant	526	1	S → P in strain: 129/SvJ.
	Natural variant	536	1	A → V in strain: 129/SvJ.

Sequences

	Sequence		Length	Mass (Da)
	Q10470 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: E5B7CF71C2022994	FASTA	538	62,003
	10 20 30 40 50 60 MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLISSFF WNNAPVTPQA 70 80 90 100 110 120 SPEPGDPDLL RTPLYSHSPL LQPLSPSKAT EELHRVDFVL PEDTTEYFVR TKAGGVCFKP 130 140 150 160 170 180 GTRMLEKPSP GRTEEKTEVS EGSSARGPAR RPMRHVLSSR ERLGSRGTRR KWVECVCLPG 190 200 210 220 230 240 WHGPSCGVPT VVQYSNLPTK ERLVPREVPR RVINAININH EFDLLDVRFH ELGDVVDAFV 250 260 270 280 290 300 VCDSNFTAYG EPRPLKFREM LTNGTFEYIR HKVLYVFLDH FPPGGRQDGW IADDYLRTFL 310 320 330 340 350 360 TQDGVSRLRN LRPDDVFIID DADEIPARDG VLFLKLYDGW TEPFAFHMRK SLYGFFWKQP 370 380 390 400 410 420 GTLEVVSGCT MDMLQAVYGL DGIRLRRRQY YTMPNFRQYE NRTGHILVQW SLGSPLHFAG 430 440 450 460 470 480 WHCSWCFTPE GIYFKLVSAQ NGDFPRWGDY EDKRDLNYIR SLIRTGGWFD GTQQEYPPAD 490 500 510 520 530 PSEHMYAPKY LLKNYDQFRY LLENPYREPK STVEGGRQNQ GSDGRSSAVR GKLDTAEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of the mouse Mgat3 gene encoding N-acetylglucosaminyltransferase III." Bhaumik M., Seldin M.F., Stanley P. Gene 164:295-300(1995) [PubMed: 7590346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Liver.
[2]	"Isolation, characterization and inactivation of the mouse Mgat3 gene: the bisecting N-acetylglucosamine in asparagine-linked oligosaccharides appears dispensable for viability and reproduction." Priatel J.J., Sarkar M., Schachter H., Marth J.D. Glycobiology 7:45-56(1997) [PubMed: 9061364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Liver.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-265, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

beta-1,4-mannosyl-glycoprotein beta 1,4-GlcNAc T III (Mgat3)

Cross-references

Sequence databases
EMBL GenBank DDBJ	L39373 Genomic DNA. Translation: AAB71422.1. Different initiation. U66844 Genomic DNA. Translation: AAC53064.1.
IPI	IPI00130260.
PIR	JC4362.
UniGene	Mm.299693.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q10470.
Protein family/group databases
CAZy	GT17. Glycosyltransferase Family 17.
PTM databases
PhosphoSite	Q10470.
Proteomic databases
PRIDE	Q10470.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:104532. Mgat3.
Phylogenomic databases
eggNOG	roNOG09700.
HOGENOM	HBG446579.
HOVERGEN	HBG052468.
InParanoid	Q10470.
OrthoDB	EOG4FFD1N.
Gene expression databases
ArrayExpress	Q10470.
Bgee	Q10470.
CleanEx	MM_MGAT3.
Genevestigator	Q10470.
GermOnline	ENSMUSG00000042428. Mus musculus.
Family and domain databases
InterPro	IPR006813. Glyco_trans_17. [Graphical view]
PANTHER	PTHR12224. Glyco_trans_17. 1 hit.
Pfam	PF04724. Glyco_transf_17. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

MGAT3_MOUSE

Accession

Primary (citable) accession number: Q10470
Secondary accession number(s): P70386

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 15, 2005
Last modified:	September 21, 2011
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program