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Q10468 (B4GN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1

EC=2.4.1.92
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene names
Name:B4galnt1
Synonyms:Galgt, Galgt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the biosynthesis of gangliosides GM2, GD2 and asialo-GM2.

Catalytic activity

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

Strongly expressed in brain, testis, spleen, and to a lesser extent in liver.

Sequence similarities

Belongs to the glycosyltransferase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1
PRO_0000059102

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2518Helical; Signal-anchor for type II membrane protein; Potential
Topological domain26 – 533508Lumenal Potential

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation2741N-linked (GlcNAc...) Potential
Disulfide bond80Interchain (with C-412) By similarity
Disulfide bond82Interchain (with C-529) By similarity
Disulfide bond412Interchain (with C-80) By similarity
Disulfide bond429 ↔ 476 By similarity
Disulfide bond529Interchain (with C-82) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q10468 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 43EF653FB07EA651

FASTA53359,279
        10         20         30         40         50         60 
MRLDRRALYA LVLLLACASL GLLYASTRDA PGLPNPLALW SPPQGPPRLD LLDLATEPRY 

        70         80         90        100        110        120 
AHIPVRIKEQ VVGLLAQNNC SCESSGGRFA LPFLRQVRAI DFTKAFDAEE LRAVSISREQ 

       130        140        150        160        170        180 
EYQAFLARSR SLADQLLIAP ANSPLQYPLQ GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL 

       190        200        210        220        230        240 
IASLGTWDVA GEVTGVTLTG EGQSDLTLAS PILDKLNRQL QLVTYSSRSY QANTADTVRF 

       250        260        270        280        290        300 
STKGHEVAFT ILIRHPPNPR LYPPSSLPQG AQYNISALVT VATKTFLRYD RLRALIASIR 

       310        320        330        340        350        360 
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK YVLWVDDDFV 

       370        380        390        400        410        420 
FTARTRLEKL VDVLERTPLD LVGGAVREIS GYATTYRQLL SVEPGAPGFG NCLRQKQGFH 

       430        440        450        460        470        480 
HELAGFPNCV VTDGVVNFFL ARTDKVRQVG FDPRLNRVAH LEFFLDGLGS LRVGSCSDVV 

       490        500        510        520        530 
VDHASKVKLP WTSKDPGAEL YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE 

« Hide

References

« Hide 'large scale' references
[1]"Beta-1,4-N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta-1,4-N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F."
Hidari J.K., Ichikawa S., Furukawa K., Yamasaki M., Hirabayashi Y.
Biochem. J. 303:957-965(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17809 mRNA. Translation: BAA04632.1.
BC081799 mRNA. Translation: AAH81799.1.
PIRS53320.
RefSeqNP_074051.1. NM_022860.2.
XP_006241560.1. XM_006241498.1.
UniGeneRn.10119.

3D structure databases

ProteinModelPortalQ10468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4576709.
STRING10116.ENSRNOP00000048586.

Protein family/group databases

CAZyGT12. Glycosyltransferase Family 12.

Proteomic databases

PaxDbQ10468.
PRIDEQ10468.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839.
GeneID64828.
KEGGrno:64828.
UCSCRGD:620490. rat.

Organism-specific databases

CTD2583.
RGD620490. B4galnt1.

Phylogenomic databases

eggNOGNOG40821.
GeneTreeENSGT00390000006679.
HOGENOMHOG000220853.
HOVERGENHBG004812.
KOK00725.
OMAIEHYLMP.
OrthoDBEOG7R2BJS.
PhylomeDBQ10468.
TreeFamTF332297.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ10468.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio613742.
PROQ10468.

Entry information

Entry nameB4GN1_RAT
AccessionPrimary (citable) accession number: Q10468
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways