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Q10468

- B4GN1_RAT

UniProt

Q10468 - B4GN1_RAT

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Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene
B4galnt1, Galgt, Galgt1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of gangliosides GM2, GD2 and asialo-GM2.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

Pathwayi

GO - Molecular functioni

  1. (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
  2. acetylgalactosaminyltransferase activity Source: RGD

GO - Biological processi

  1. ganglioside biosynthetic process Source: Ensembl
  2. lipid glycosylation Source: InterPro
  3. lipid storage Source: Ensembl
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT12. Glycosyltransferase Family 12.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.92)
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene namesi
Name:B4galnt1
Synonyms:Galgt, Galgt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi620490. B4galnt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Transmembranei8 – 2518Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini26 – 533508Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
  2. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1PRO_0000059102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi80 – 80Interchain (with C-412) By similarity
Disulfide bondi82 – 82Interchain (with C-529) By similarity
Glycosylationi274 – 2741N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi412 – 412Interchain (with C-80) By similarity
Disulfide bondi429 ↔ 476 By similarity
Disulfide bondi529 – 529Interchain (with C-82) By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ10468.
PRIDEiQ10468.

Expressioni

Tissue specificityi

Strongly expressed in brain, testis, spleen, and to a lesser extent in liver.

Gene expression databases

GenevestigatoriQ10468.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Protein-protein interaction databases

MINTiMINT-4576709.
STRINGi10116.ENSRNOP00000048586.

Structurei

3D structure databases

ProteinModelPortaliQ10468.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40821.
GeneTreeiENSGT00390000006679.
HOGENOMiHOG000220853.
HOVERGENiHBG004812.
KOiK00725.
OMAiIEHYLMP.
OrthoDBiEOG7R2BJS.
PhylomeDBiQ10468.
TreeFamiTF332297.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10468-1 [UniParc]FASTAAdd to Basket

« Hide

MRLDRRALYA LVLLLACASL GLLYASTRDA PGLPNPLALW SPPQGPPRLD    50
LLDLATEPRY AHIPVRIKEQ VVGLLAQNNC SCESSGGRFA LPFLRQVRAI 100
DFTKAFDAEE LRAVSISREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ 150
GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL IASLGTWDVA GEVTGVTLTG 200
EGQSDLTLAS PILDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT 250
ILIRHPPNPR LYPPSSLPQG AQYNISALVT VATKTFLRYD RLRALIASIR 300
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK 350
YVLWVDDDFV FTARTRLEKL VDVLERTPLD LVGGAVREIS GYATTYRQLL 400
SVEPGAPGFG NCLRQKQGFH HELAGFPNCV VTDGVVNFFL ARTDKVRQVG 450
FDPRLNRVAH LEFFLDGLGS LRVGSCSDVV VDHASKVKLP WTSKDPGAEL 500
YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE 533
Length:533
Mass (Da):59,279
Last modified:October 1, 1996 - v1
Checksum:i43EF653FB07EA651
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17809 mRNA. Translation: BAA04632.1.
BC081799 mRNA. Translation: AAH81799.1.
PIRiS53320.
RefSeqiNP_074051.1. NM_022860.2.
XP_006241560.1. XM_006241498.1.
UniGeneiRn.10119.

Genome annotation databases

EnsembliENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839.
GeneIDi64828.
KEGGirno:64828.
UCSCiRGD:620490. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17809 mRNA. Translation: BAA04632.1 .
BC081799 mRNA. Translation: AAH81799.1 .
PIRi S53320.
RefSeqi NP_074051.1. NM_022860.2.
XP_006241560.1. XM_006241498.1.
UniGenei Rn.10119.

3D structure databases

ProteinModelPortali Q10468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4576709.
STRINGi 10116.ENSRNOP00000048586.

Protein family/group databases

CAZyi GT12. Glycosyltransferase Family 12.

Proteomic databases

PaxDbi Q10468.
PRIDEi Q10468.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000046099 ; ENSRNOP00000048586 ; ENSRNOG00000004839 .
GeneIDi 64828.
KEGGi rno:64828.
UCSCi RGD:620490. rat.

Organism-specific databases

CTDi 2583.
RGDi 620490. B4galnt1.

Phylogenomic databases

eggNOGi NOG40821.
GeneTreei ENSGT00390000006679.
HOGENOMi HOG000220853.
HOVERGENi HBG004812.
KOi K00725.
OMAi IEHYLMP.
OrthoDBi EOG7R2BJS.
PhylomeDBi Q10468.
TreeFami TF332297.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 613742.
PROi Q10468.

Gene expression databases

Genevestigatori Q10468.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Beta-1,4-N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta-1,4-N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F."
    Hidari J.K., Ichikawa S., Furukawa K., Yamasaki M., Hirabayashi Y.
    Biochem. J. 303:957-965(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiB4GN1_RAT
AccessioniPrimary (citable) accession number: Q10468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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