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Q10468

- B4GN1_RAT

UniProt

Q10468 - B4GN1_RAT

Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene

B4galnt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of gangliosides GM2, GD2 and asialo-GM2.

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

    Pathwayi

    GO - Molecular functioni

    1. (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
    2. acetylgalactosaminyltransferase activity Source: RGD

    GO - Biological processi

    1. ganglioside biosynthetic process Source: Ensembl
    2. lipid glycosylation Source: InterPro
    3. lipid storage Source: Ensembl
    4. protein glycosylation Source: UniProtKB-UniPathway
    5. spermatogenesis Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT12. Glycosyltransferase Family 12.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.92)
    Alternative name(s):
    (N-acetylneuraminyl)-galactosylglucosylceramide
    GM2/GD2 synthase
    GalNAc-T
    Gene namesi
    Name:B4galnt1
    Synonyms:Galgt, Galgt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi620490. B4galnt1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of Golgi membrane Source: InterPro
    2. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1PRO_0000059102Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi80 – 80Interchain (with C-412)By similarity
    Disulfide bondi82 – 82Interchain (with C-529)By similarity
    Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi412 – 412Interchain (with C-80)By similarity
    Disulfide bondi429 ↔ 476By similarity
    Disulfide bondi529 – 529Interchain (with C-82)By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ10468.
    PRIDEiQ10468.

    Expressioni

    Tissue specificityi

    Strongly expressed in brain, testis, spleen, and to a lesser extent in liver.

    Gene expression databases

    GenevestigatoriQ10468.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    MINTiMINT-4576709.
    STRINGi10116.ENSRNOP00000048586.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10468.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini26 – 533508LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2518Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40821.
    GeneTreeiENSGT00390000006679.
    HOGENOMiHOG000220853.
    HOVERGENiHBG004812.
    KOiK00725.
    OMAiIEHYLMP.
    OrthoDBiEOG7R2BJS.
    PhylomeDBiQ10468.
    TreeFamiTF332297.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR011143. GM2_synthase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q10468-1 [UniParc]FASTAAdd to Basket

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    MRLDRRALYA LVLLLACASL GLLYASTRDA PGLPNPLALW SPPQGPPRLD    50
    LLDLATEPRY AHIPVRIKEQ VVGLLAQNNC SCESSGGRFA LPFLRQVRAI 100
    DFTKAFDAEE LRAVSISREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ 150
    GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL IASLGTWDVA GEVTGVTLTG 200
    EGQSDLTLAS PILDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT 250
    ILIRHPPNPR LYPPSSLPQG AQYNISALVT VATKTFLRYD RLRALIASIR 300
    RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK 350
    YVLWVDDDFV FTARTRLEKL VDVLERTPLD LVGGAVREIS GYATTYRQLL 400
    SVEPGAPGFG NCLRQKQGFH HELAGFPNCV VTDGVVNFFL ARTDKVRQVG 450
    FDPRLNRVAH LEFFLDGLGS LRVGSCSDVV VDHASKVKLP WTSKDPGAEL 500
    YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE 533
    Length:533
    Mass (Da):59,279
    Last modified:October 1, 1996 - v1
    Checksum:i43EF653FB07EA651
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17809 mRNA. Translation: BAA04632.1.
    BC081799 mRNA. Translation: AAH81799.1.
    PIRiS53320.
    RefSeqiNP_074051.1. NM_022860.2.
    XP_006241560.1. XM_006241498.1.
    UniGeneiRn.10119.

    Genome annotation databases

    EnsembliENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839.
    GeneIDi64828.
    KEGGirno:64828.
    UCSCiRGD:620490. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17809 mRNA. Translation: BAA04632.1 .
    BC081799 mRNA. Translation: AAH81799.1 .
    PIRi S53320.
    RefSeqi NP_074051.1. NM_022860.2.
    XP_006241560.1. XM_006241498.1.
    UniGenei Rn.10119.

    3D structure databases

    ProteinModelPortali Q10468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4576709.
    STRINGi 10116.ENSRNOP00000048586.

    Protein family/group databases

    CAZyi GT12. Glycosyltransferase Family 12.

    Proteomic databases

    PaxDbi Q10468.
    PRIDEi Q10468.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000046099 ; ENSRNOP00000048586 ; ENSRNOG00000004839 .
    GeneIDi 64828.
    KEGGi rno:64828.
    UCSCi RGD:620490. rat.

    Organism-specific databases

    CTDi 2583.
    RGDi 620490. B4galnt1.

    Phylogenomic databases

    eggNOGi NOG40821.
    GeneTreei ENSGT00390000006679.
    HOGENOMi HOG000220853.
    HOVERGENi HBG004812.
    KOi K00725.
    OMAi IEHYLMP.
    OrthoDBi EOG7R2BJS.
    PhylomeDBi Q10468.
    TreeFami TF332297.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 613742.
    PROi Q10468.

    Gene expression databases

    Genevestigatori Q10468.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR011143. GM2_synthase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000474. GM2_GD2_synthase. 1 hit.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Beta-1,4-N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta-1,4-N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F."
      Hidari J.K., Ichikawa S., Furukawa K., Yamasaki M., Hirabayashi Y.
      Biochem. J. 303:957-965(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiB4GN1_RAT
    AccessioniPrimary (citable) accession number: Q10468
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3