Q10468 (B4GN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1
EC=2.4.1.92

Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T

Gene names

Name:	B4galnt1
Synonyms:	Galgt, Galgt1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the biosynthesis of gangliosides GM2, GD2 and asialo-GM2.
Catalytic activity	UDP-N-acetyl-D-galactosamine + 1-O-(O-(N-acetyl-alpha-neuraminosyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl)-ceramide = UDP + 1-O-(O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminosyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Strongly expressed in brain, testis, spleen, and to a lesser extent in liver.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay Ref.1. Source: RGD
Molecular function	(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Beta-1,4 N-acetylgalactosaminyltransferase 1		PRO_0000059102
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 25	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 533	508	Lumenal Potential
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Disulfide bond	80		Interchain (with C-412) By similarity
	Disulfide bond	82		Interchain (with C-529) By similarity
	Disulfide bond	412		Interchain (with C-80) By similarity
	Disulfide bond	429 ↔ 476		By similarity
	Disulfide bond	529		Interchain (with C-82) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10468 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 43EF653FB07EA651	FASTA	533	59,279
	10 20 30 40 50 60 MRLDRRALYA LVLLLACASL GLLYASTRDA PGLPNPLALW SPPQGPPRLD LLDLATEPRY 70 80 90 100 110 120 AHIPVRIKEQ VVGLLAQNNC SCESSGGRFA LPFLRQVRAI DFTKAFDAEE LRAVSISREQ 130 140 150 160 170 180 EYQAFLARSR SLADQLLIAP ANSPLQYPLQ GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL 190 200 210 220 230 240 IASLGTWDVA GEVTGVTLTG EGQSDLTLAS PILDKLNRQL QLVTYSSRSY QANTADTVRF 250 260 270 280 290 300 STKGHEVAFT ILIRHPPNPR LYPPSSLPQG AQYNISALVT VATKTFLRYD RLRALIASIR 310 320 330 340 350 360 RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK YVLWVDDDFV 370 380 390 400 410 420 FTARTRLEKL VDVLERTPLD LVGGAVREIS GYATTYRQLL SVEPGAPGFG NCLRQKQGFH 430 440 450 460 470 480 HELAGFPNCV VTDGVVNFFL ARTDKVRQVG FDPRLNRVAH LEFFLDGLGS LRVGSCSDVV 490 500 510 520 530 VDHASKVKLP WTSKDPGAEL YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-1,4-N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta-1,4-N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F." Hidari J.K., Ichikawa S., Furukawa K., Yamasaki M., Hirabayashi Y. Biochem. J. 303:957-965(1994) [PubMed: 7980468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17809 mRNA. Translation: BAA04632.1. BC081799 mRNA. Translation: AAH81799.1.
IPI	IPI00208973.
PIR	S53320.
RefSeq	NP_074051.1. NM_022860.2.
UniGene	Rn.10119.
3D structure databases
ProteinModelPortal	Q10468.
ModBase	Search...
Protein-protein interaction databases
STRING	Q10468.
Protein family/group databases
CAZy	GT12. Glycosyltransferase Family 12.
Proteomic databases
PRIDE	Q10468.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839.
GeneID	64828.
KEGG	rno:64828.
UCSC	NM_022860. rat.
Organism-specific databases
CTD	2583.
RGD	620490. B4galnt1.
Phylogenomic databases
eggNOG	roNOG05366.
GeneTree	ENSGT00390000006679.
HOVERGEN	HBG004812.
OrthoDB	EOG4WH8KK.
Gene expression databases
ArrayExpress	Q10468.
Genevestigator	Q10468.
GermOnline	ENSRNOG00000004839. Rattus norvegicus.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR011143. GM2_synthase. [Graphical view]
KO	K00725.
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF000474. GM2_GD2_synthase. 1 hit.
ProtoNet	Search...
Other
NextBio	613742.

Entry information

Entry name

B4GN1_RAT

Accession

Primary (citable) accession number: Q10468

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program