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Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene

B4galnt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of gangliosides GM2, GD2 and asialo-GM2.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.1 Publication

Pathwayi: Sphingolipid metabolism

This protein is involved in Sphingolipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in Sphingolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-1660662 Glycosphingolipid metabolism

Protein family/group databases

CAZyiGT12 Glycosyltransferase Family 12

Chemistry databases

SwissLipidsiSLP:000000769

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.921 Publication)
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene namesi
Name:B4galnt1
Synonyms:Galgt, Galgt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620490 B4galnt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini26 – 533LumenalSequence analysisAdd BLAST508

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591021 – 533Beta-1,4 N-acetylgalactosaminyltransferase 1Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi80Interchain (with C-412)By similarity
Disulfide bondi82Interchain (with C-529)By similarity
Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi412Interchain (with C-80)By similarity
Disulfide bondi429 ↔ 476By similarity
Disulfide bondi529Interchain (with C-82)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ10468
PRIDEiQ10468

Expressioni

Tissue specificityi

Strongly expressed in brain, testis, spleen, and to a lesser extent in liver.

Gene expression databases

BgeeiENSRNOG00000004839
GenevisibleiQ10468 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048586

Structurei

3D structure databases

ProteinModelPortaliQ10468
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF61 Eukaryota
ENOG4111N56 LUCA
GeneTreeiENSGT00390000006679
HOGENOMiHOG000220853
HOVERGENiHBG004812
InParanoidiQ10468
KOiK00725
OMAiTYAKFRY
OrthoDBiEOG091G04CW
PhylomeDBiQ10468
TreeFamiTF332297

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR011143 GM2_synthase
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PIRSFiPIRSF000474 GM2_GD2_synthase, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q10468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLDRRALYA LVLLLACASL GLLYASTRDA PGLPNPLALW SPPQGPPRLD
60 70 80 90 100
LLDLATEPRY AHIPVRIKEQ VVGLLAQNNC SCESSGGRFA LPFLRQVRAI
110 120 130 140 150
DFTKAFDAEE LRAVSISREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ
160 170 180 190 200
GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL IASLGTWDVA GEVTGVTLTG
210 220 230 240 250
EGQSDLTLAS PILDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT
260 270 280 290 300
ILIRHPPNPR LYPPSSLPQG AQYNISALVT VATKTFLRYD RLRALIASIR
310 320 330 340 350
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK
360 370 380 390 400
YVLWVDDDFV FTARTRLEKL VDVLERTPLD LVGGAVREIS GYATTYRQLL
410 420 430 440 450
SVEPGAPGFG NCLRQKQGFH HELAGFPNCV VTDGVVNFFL ARTDKVRQVG
460 470 480 490 500
FDPRLNRVAH LEFFLDGLGS LRVGSCSDVV VDHASKVKLP WTSKDPGAEL
510 520 530
YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE
Length:533
Mass (Da):59,279
Last modified:October 1, 1996 - v1
Checksum:i43EF653FB07EA651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17809 mRNA Translation: BAA04632.1
BC081799 mRNA Translation: AAH81799.1
PIRiS53320
RefSeqiNP_074051.1, NM_022860.2
XP_017450589.1, XM_017595100.1
UniGeneiRn.10119

Genome annotation databases

EnsembliENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839
GeneIDi64828
KEGGirno:64828
UCSCiRGD:620490 rat

Similar proteinsi

Entry informationi

Entry nameiB4GN1_RAT
AccessioniPrimary (citable) accession number: Q10468
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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