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Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene

B4galnt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of gangliosides GM2, GD2 and asialo-GM2.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

Pathwayi

GO - Molecular functioni

  1. (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
  2. acetylgalactosaminyltransferase activity Source: RGD

GO - Biological processi

  1. ganglioside biosynthetic process Source: Ensembl
  2. lipid glycosylation Source: InterPro
  3. lipid storage Source: Ensembl
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT12. Glycosyltransferase Family 12.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.92)
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene namesi
Name:B4galnt1
Synonyms:Galgt, Galgt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620490. B4galnt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2518Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini26 – 533508LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
  2. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1PRO_0000059102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi80 – 80Interchain (with C-412)By similarity
Disulfide bondi82 – 82Interchain (with C-529)By similarity
Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi412 – 412Interchain (with C-80)By similarity
Disulfide bondi429 ↔ 476By similarity
Disulfide bondi529 – 529Interchain (with C-82)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ10468.
PRIDEiQ10468.

Expressioni

Tissue specificityi

Strongly expressed in brain, testis, spleen, and to a lesser extent in liver.

Gene expression databases

GenevestigatoriQ10468.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

MINTiMINT-4576709.
STRINGi10116.ENSRNOP00000048586.

Structurei

3D structure databases

ProteinModelPortaliQ10468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40821.
GeneTreeiENSGT00390000006679.
HOGENOMiHOG000220853.
HOVERGENiHBG004812.
InParanoidiQ10468.
KOiK00725.
OMAiIEHYLMP.
OrthoDBiEOG7R2BJS.
PhylomeDBiQ10468.
TreeFamiTF332297.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLDRRALYA LVLLLACASL GLLYASTRDA PGLPNPLALW SPPQGPPRLD
60 70 80 90 100
LLDLATEPRY AHIPVRIKEQ VVGLLAQNNC SCESSGGRFA LPFLRQVRAI
110 120 130 140 150
DFTKAFDAEE LRAVSISREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ
160 170 180 190 200
GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL IASLGTWDVA GEVTGVTLTG
210 220 230 240 250
EGQSDLTLAS PILDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT
260 270 280 290 300
ILIRHPPNPR LYPPSSLPQG AQYNISALVT VATKTFLRYD RLRALIASIR
310 320 330 340 350
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK
360 370 380 390 400
YVLWVDDDFV FTARTRLEKL VDVLERTPLD LVGGAVREIS GYATTYRQLL
410 420 430 440 450
SVEPGAPGFG NCLRQKQGFH HELAGFPNCV VTDGVVNFFL ARTDKVRQVG
460 470 480 490 500
FDPRLNRVAH LEFFLDGLGS LRVGSCSDVV VDHASKVKLP WTSKDPGAEL
510 520 530
YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE
Length:533
Mass (Da):59,279
Last modified:September 30, 1996 - v1
Checksum:i43EF653FB07EA651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17809 mRNA. Translation: BAA04632.1.
BC081799 mRNA. Translation: AAH81799.1.
PIRiS53320.
RefSeqiNP_074051.1. NM_022860.2.
UniGeneiRn.10119.

Genome annotation databases

EnsembliENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839.
GeneIDi64828.
KEGGirno:64828.
UCSCiRGD:620490. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17809 mRNA. Translation: BAA04632.1.
BC081799 mRNA. Translation: AAH81799.1.
PIRiS53320.
RefSeqiNP_074051.1. NM_022860.2.
UniGeneiRn.10119.

3D structure databases

ProteinModelPortaliQ10468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4576709.
STRINGi10116.ENSRNOP00000048586.

Protein family/group databases

CAZyiGT12. Glycosyltransferase Family 12.

Proteomic databases

PaxDbiQ10468.
PRIDEiQ10468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000046099; ENSRNOP00000048586; ENSRNOG00000004839.
GeneIDi64828.
KEGGirno:64828.
UCSCiRGD:620490. rat.

Organism-specific databases

CTDi2583.
RGDi620490. B4galnt1.

Phylogenomic databases

eggNOGiNOG40821.
GeneTreeiENSGT00390000006679.
HOGENOMiHOG000220853.
HOVERGENiHBG004812.
InParanoidiQ10468.
KOiK00725.
OMAiIEHYLMP.
OrthoDBiEOG7R2BJS.
PhylomeDBiQ10468.
TreeFamiTF332297.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi613742.
PROiQ10468.

Gene expression databases

GenevestigatoriQ10468.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Beta-1,4-N-acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a beta-1,4-N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F."
    Hidari J.K., Ichikawa S., Furukawa K., Yamasaki M., Hirabayashi Y.
    Biochem. J. 303:957-965(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiB4GN1_RAT
AccessioniPrimary (citable) accession number: Q10468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: January 6, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.