Reviewed, UniProtKB/Swiss-Prot Q10457 (PUR6_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable multifunctional protein ADE2
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazole-succinocarboxamide synthase
              EC=6.3.2.6
        Alternative name(s):
            SAICAR synthetase
    2- Recommended name:
            Phosphoribosylaminoimidazole carboxylase
              EC=4.1.1.21
        Alternative name(s):
            AIR carboxylase
              Short name=AIRC

Gene names

ORF Names:

B0286.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 3/5. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 4/5.
Sequence similarities	In the N-terminal section; belongs to the SAICAR synthetase family. In the C-terminal section; belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Ligase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-311886,EBI-311886

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Probable multifunctional protein ADE2		PRO_0000075034
Regions
	Region	1 – 263	263	SAICAR synthetase
	Region	264 – 423	160	AIR carboxylase

Sequences

	Sequence		Length	Mass (Da)
	Q10457-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 101CD65318C4804A	FASTA	423	46,978
	10 20 30 40 50 60 MSSLAEIASR PENLELLAEG KTKQIFDIKG EKDYVLIRSK DSLTAFNAVR KNELEGKSRI 70 80 90 100 110 120 ASKTTSNVFE YLQLLGLPTH FEKSISETEF VARKCTMIPI EWVARRVATG SFLKRNPGVK 130 140 150 160 170 180 EGFRFNDLKL ETFFKDDAND DPQWTDEQIV SNGLMIDHLK IGREEISLMK KMTKLVFRAL 190 200 210 220 230 240 EKGWALSNSA LIDMKIEFGV TVEGEILLAD VIDNDSWRVW PENDRRLQLD KQVYRDMKEV 250 260 270 280 290 300 TEEGLALVLK NYTKVMDITA TFSKHQQKCH VLVIMGSGSD GVFARKISDE AKKFGLETTL 310 320 330 340 350 360 KVSSAHKTTS DTLEVIADFE ESGVPTVVIA VAGRSNGLGP VIAGNSSLPV INCPPPSEST 370 380 390 400 410 420 SLDIWSSLRM PNGIGCTTVL DPSEAALAAA KILASHNHIV FGKVLTAQLK NQINIYNANR KLE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U39848 Genomic DNA. Translation: AAA80690.1.
PIR	T15309.
RefSeq	NP_494771.1.
UniGene	Cel.16061
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24510N.
IntAct	Q10457. 1 interaction.
Genome annotation databases
Ensembl	B0286.3. Caenorhabditis elegans. [Contig view]
GeneID	173772.
KEGG	cel:B0286.3.
NMPDR	fig|6239.3.peg.5157.
Organism-specific databases
WormBase	WBGene00015116. B0286.3.
WormPep	B0286.3. CE03863. [WorfDB]
Phylogenomic databases
OMA	Q10457. SQCRVVV.
Enzyme and pathway databases
BRENDA	4.1.1.21. 672. 6.3.2.6. 672.
Gene expression databases
ArrayExpress	Q10457.
Family and domain databases
InterPro	IPR000031. AIR_COase_core. IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF01259. SAICAR_synt. 1 hit. [Graphical view]
ProDom	PD002193. AIR_carboxyl. 1 hit. PD003043. SAICAR_synt. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	881027.

Entry information

Entry name

PUR6_CAEEL

Accession

Primary (citable) accession number: Q10457

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project