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Protein

Probable multifunctional protein ADE2

Gene

B0286.3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable multifunctional protein ADE2 (B0286.3)
  2. Adenylosuccinate lyase (adsl-1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Probable multifunctional protein ADE2 (B0286.3)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00130.
UPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable multifunctional protein ADE2
Including the following 2 domains:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
ORF Names:B0286.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiB0286.3; CE03863; WBGene00015116.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000750341 – 423Probable multifunctional protein ADE2Add BLAST423

Proteomic databases

EPDiQ10457.
PaxDbiQ10457.
PeptideAtlasiQ10457.
PRIDEiQ10457.

Expressioni

Gene expression databases

BgeeiWBGene00015116.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-311886,EBI-311886

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi39128. 2 interactors.
DIPiDIP-24510N.
IntActiQ10457. 20 interactors.
MINTiMINT-1086946.
STRINGi6239.B0286.3.

Structurei

3D structure databases

ProteinModelPortaliQ10457.
SMRiQ10457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 263SAICAR synthetaseAdd BLAST263
Regioni264 – 423AIR carboxylaseAdd BLAST160

Sequence similaritiesi

In the N-terminal section; belongs to the SAICAR synthetase family.Curated
In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.Curated

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0041. LUCA.
COG0152. LUCA.
GeneTreeiENSGT00390000010172.
HOGENOMiHOG000082628.
InParanoidiQ10457.
KOiK01587.
OMAiWQTKNCA.
OrthoDBiEOG091G08DN.
PhylomeDBiQ10457.

Family and domain databases

Gene3Di3.40.50.7700. 1 hit.
HAMAPiMF_02045. PurE_classII. 1 hit.
MF_00137. SAICAR_synth. 1 hit.
InterProiView protein in InterPro
IPR033626. PurE_classII.
IPR000031. PurE_dom.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
PfamiView protein in Pfam
PF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
SMARTiView protein in SMART
SM01001. AIRC. 1 hit.
SUPFAMiSSF52255. SSF52255. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
PROSITEiView protein in PROSITE
PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLAEIASR PENLELLAEG KTKQIFDIKG EKDYVLIRSK DSLTAFNAVR
60 70 80 90 100
KNELEGKSRI ASKTTSNVFE YLQLLGLPTH FEKSISETEF VARKCTMIPI
110 120 130 140 150
EWVARRVATG SFLKRNPGVK EGFRFNDLKL ETFFKDDAND DPQWTDEQIV
160 170 180 190 200
SNGLMIDHLK IGREEISLMK KMTKLVFRAL EKGWALSNSA LIDMKIEFGV
210 220 230 240 250
TVEGEILLAD VIDNDSWRVW PENDRRLQLD KQVYRDMKEV TEEGLALVLK
260 270 280 290 300
NYTKVMDITA TFSKHQQKCH VLVIMGSGSD GVFARKISDE AKKFGLETTL
310 320 330 340 350
KVSSAHKTTS DTLEVIADFE ESGVPTVVIA VAGRSNGLGP VIAGNSSLPV
360 370 380 390 400
INCPPPSEST SLDIWSSLRM PNGIGCTTVL DPSEAALAAA KILASHNHIV
410 420
FGKVLTAQLK NQINIYNANR KLE
Length:423
Mass (Da):46,978
Last modified:October 1, 1996 - v1
Checksum:i101CD65318C4804A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080154 Genomic DNA. Translation: CCD61663.1.
PIRiT15309.
RefSeqiNP_494771.1. NM_062370.5.
UniGeneiCel.16061.

Genome annotation databases

EnsemblMetazoaiB0286.3; B0286.3; WBGene00015116.
GeneIDi173772.
KEGGicel:CELE_B0286.3.
UCSCiB0286.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080154 Genomic DNA. Translation: CCD61663.1.
PIRiT15309.
RefSeqiNP_494771.1. NM_062370.5.
UniGeneiCel.16061.

3D structure databases

ProteinModelPortaliQ10457.
SMRiQ10457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39128. 2 interactors.
DIPiDIP-24510N.
IntActiQ10457. 20 interactors.
MINTiMINT-1086946.
STRINGi6239.B0286.3.

Proteomic databases

EPDiQ10457.
PaxDbiQ10457.
PeptideAtlasiQ10457.
PRIDEiQ10457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0286.3; B0286.3; WBGene00015116.
GeneIDi173772.
KEGGicel:CELE_B0286.3.
UCSCiB0286.3. c. elegans.

Organism-specific databases

CTDi173772.
WormBaseiB0286.3; CE03863; WBGene00015116.

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0041. LUCA.
COG0152. LUCA.
GeneTreeiENSGT00390000010172.
HOGENOMiHOG000082628.
InParanoidiQ10457.
KOiK01587.
OMAiWQTKNCA.
OrthoDBiEOG091G08DN.
PhylomeDBiQ10457.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00130.
UPA00074; UER00131.
ReactomeiR-CEL-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiPR:Q10457.

Gene expression databases

BgeeiWBGene00015116.

Family and domain databases

Gene3Di3.40.50.7700. 1 hit.
HAMAPiMF_02045. PurE_classII. 1 hit.
MF_00137. SAICAR_synth. 1 hit.
InterProiView protein in InterPro
IPR033626. PurE_classII.
IPR000031. PurE_dom.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
PfamiView protein in Pfam
PF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
SMARTiView protein in SMART
SM01001. AIRC. 1 hit.
SUPFAMiSSF52255. SSF52255. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
PROSITEiView protein in PROSITE
PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUR6_CAEEL
AccessioniPrimary (citable) accession number: Q10457
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 10, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.