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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

SPAC25G10.08

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72689. Formation of a pool of free 40S subunits.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 90 kDa subunit homologUniRule annotation
Short name:
eIF3 p90UniRule annotation
Translation initiation factor eIF3 p90 subunit homolog
spPrt1
Gene namesi
ORF Names:SPAC25G10.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC25G10.08.
PomBaseiSPAC25G10.08.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: PomBase
  • eukaryotic 43S preinitiation complex Source: PomBase
  • eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic translation initiation factor 3 complex, eIF3e Source: PomBase
  • eukaryotic translation initiation factor 3 complex, eIF3m Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Eukaryotic translation initiation factor 3 subunit BPRO_0000123534Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231PhosphoserineUniRule annotation1 Publication
Modified residuei135 – 1351PhosphoserineUniRule annotation1 Publication
Modified residuei136 – 1361Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10425.

PTM databases

iPTMnetiQ10425.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex appears to include tif32/eif3a, SPAC25G10.08/eif3b, tif33/eif3c, SPBC4C3.07/eif3f, tif35/eif3g and sum1/eif3i. This set of common subunits may also associate exclusively with either moe1/eif3d and int6/eif3e, or with SPAC821.05/eif3h and SPAC1751.03/eif3m. The eIF-3 complex may also include SPAC3A12.13c/eif3j.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi278567. 20 interactions.
MINTiMINT-4699310.

Structurei

3D structure databases

ProteinModelPortaliQ10425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 12991RRMUniRule annotationAdd
BLAST
Repeati190 – 22940WD 1Add
BLAST
Repeati304 – 34441WD 2Add
BLAST
Repeati347 – 38640WD 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili630 – 67142UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation
Contains 3 WD repeats.UniRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000265546.
InParanoidiQ10425.
KOiK03253.
OMAiKKTCNSL.
OrthoDBiEOG092C0KCM.
PhylomeDBiQ10425.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEILIEEIK DQIVVKDEEI DVSELEVKLN VTKPVGYDTV VVIEGAPVVE
60 70 80 90 100
EAKQQDFFRF LSSKVLAKIG KVKENGFYMP FEEKNGKKMS LGLVFADFEN
110 120 130 140 150
VDGADLCVQE LDGKQILKNH TFVVRKLNQL EKAFSTPDEF SFEEREFKER
160 170 180 190 200
EHLRSWLTDY YGRDQFISYY GNRVSVNWNR KSDVPEQIVD RENWTETYVQ
210 220 230 240 250
WSPMGTYLVS LHLRGIQLWG GESWGMCARF LHPYVKFVDF SPNEKYLVSW
260 270 280 290 300
SYEPVRLPPI GHPARETMPF TDDDEGKHCF VWDIASGRIL RSFKIPPQPE
310 320 330 340 350
GSKDGKKVIW PIFKWSADDK YLARVTVGQS ISVYETPSLA LVDKKTIKID
360 370 380 390 400
GVQNFEWCPV SDALGRDSKE QLLAYWTPEI TNQPARVALI SIPSKSTIRT
410 420 430 440 450
KNLFNVSDCK LYWQSNGDYL CVKVDRHTKT KKSTFSNLEI FRIREKNIPV
460 470 480 490 500
EVVDLKDVVL NFAWEPKSDR FAIISANDQV LNSTNVKTNL SFYGFEQKKN
510 520 530 540 550
TPSTFRHIIT FDKKTCNSLF MAPKGRFMVA ATLGSSTQYD LEFYDLDFDT
560 570 580 590 600
EKKEPDALAN VQQIGSAEHF GMTELEWDPS GRYVTTSSTI WRHKLENGYR
610 620 630 640 650
LCDFRGTLLR EEMIGEFKQF IWRPRPPSPL TKEDMKKIRK KLKDYNRLFD
660 670 680 690 700
EEDIAEQSSA NRELAARRRQ LISEWQKYRD EVIARVAEER AITGQPAITV
710 720
PAEEEEIIQE TVEEVISEEI EPVED
Length:725
Mass (Da):84,035
Last modified:October 1, 1996 - v1
Checksum:iD4725583F7F9EE34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA94637.1.
PIRiT38379.
RefSeqiNP_594528.1. NM_001019957.2.

Genome annotation databases

EnsemblFungiiSPAC25G10.08.1; SPAC25G10.08.1:pep; SPAC25G10.08.
GeneIDi2542090.
KEGGispo:SPAC25G10.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA94637.1.
PIRiT38379.
RefSeqiNP_594528.1. NM_001019957.2.

3D structure databases

ProteinModelPortaliQ10425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278567. 20 interactions.
MINTiMINT-4699310.

PTM databases

iPTMnetiQ10425.

Proteomic databases

MaxQBiQ10425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC25G10.08.1; SPAC25G10.08.1:pep; SPAC25G10.08.
GeneIDi2542090.
KEGGispo:SPAC25G10.08.

Organism-specific databases

EuPathDBiFungiDB:SPAC25G10.08.
PomBaseiSPAC25G10.08.

Phylogenomic databases

HOGENOMiHOG000265546.
InParanoidiQ10425.
KOiK03253.
OMAiKKTCNSL.
OrthoDBiEOG092C0KCM.
PhylomeDBiQ10425.

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72689. Formation of a pool of free 40S subunits.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

PROiQ10425.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3B_SCHPO
AccessioniPrimary (citable) accession number: Q10425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.