Reviewed, UniProtKB/Swiss-Prot Q10407 (MKH1_SCHPO)

Names and origin

Protein names

Recommended name:
    MAP kinase kinase kinase mkh1
    EC=2.7.11.25

Gene names

Name:	mkh1
ORF Names:	SPAC1F3.02c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1116 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May regulate cell morphology, cell wall integrity, salt resistance, cell cycle reentry from stationary-phase arrest, and filamentous growth in response to stress. Activates the MAP kinase kinase skh1/pek1 by phosphorylation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	MAPKKK cascade Inferred from direct assay. Source: GeneDB_SPombe cell morphogenesis Non-traceable author statement. Source: GeneDB_SPombe cellular cell wall organization Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cellular response to heat Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cellular sodium ion homeostasis Non-traceable author statement. Source: GeneDB_SPombe cytokinesis Non-traceable author statement. Source: GeneDB_SPombe positive regulation of calcium-mediated signaling Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to osmotic stress Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cell division site Inferred from direct assay. Source: GeneDB_SPombe cell septum Inferred from direct assay. Source: GeneDB_SPombe cell tip Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Ref.1 Traceable author statement. Source: GeneDB_SPombe protein binding Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1116	1116	MAP kinase kinase kinase mkh1		PRO_0000086331
Regions
	Domain	825 – 1094	270	Protein kinase
	Nucleotide binding	831 – 839	9	ATP By similarity
Sites
	Active site	955	1	Proton acceptor By similarity
	Binding site	854	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10407-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7AFDB3EC62EED47B	FASTA	1,116	125,133
	10 20 30 40 50 60 MAADIGSQSS GSLEERFEQS LHLQNVDKQD WSLNSVLQFL KLYKFNKEWE DVFIKSRIEM 70 80 90 100 110 120 DLFINLADQS KAEEFAFKNK LSKESAIQLS SCIRKTLLAP SSTRVPSKNS SYETLTYSAK 130 140 150 160 170 180 DSSDDVFTET NSGFRSSNQN SSLKSFQSVP DSNVNVFGGF GGSVVDNNEL LSTGKNSHQT 190 200 210 220 230 240 TSLNLEGSPI NLHAYKGTVT SIINDDSRNI NKKTLSKQPV SEHKEKQTSF LRRFRVPGFS 250 260 270 280 290 300 RDKDKTKDCP SSNSNPFHLA SSNVKTLDAS LDQGEWVPRI HRLESQIGLI SKKKSFVLAT 310 320 330 340 350 360 MDDMKFTVVD ITNVQNATQL RKLIAKSMYL DISIDQFDLF LTEVGGAQYI EILDDRKLDI 370 380 390 400 410 420 ARLYSDEFGT IKFFVKPSQN EESGMDSDTY LSFGTKSSST YKADDDSIYH RKEDFKKQPS 430 440 450 460 470 480 YPVLTSDFEI TDAGPNLSLS GHQPDNKYYK GFSSAPNLAV VPELPSRRFR GFEKIRGAKG 490 500 510 520 530 540 EMATKILDAT EAQSEKNKFT VCRPHKKVTL KMPLNSGSSA PQSPSSNTSA SVLTRNFVAH 550 560 570 580 590 600 RDPPPPPTET SSLRRKNTLT RRPSIRHARS SPYIDTGHNE ASKFSHTSFD PKASSKSSNS 610 620 630 640 650 660 LKESVEALSE IPFEDAPALD ESDLSGDPFW AIQPKQSSSQ VPKENHHNIQ SKLSINTEAA 670 680 690 700 710 720 TDLKANELSS PKTPEYCRGD DRSISLSPLS YRLRKSKHIR ESPPSSKVIN SGNWEVRPSA 730 740 750 760 770 780 DDLYEDVDRF FPRYDLDKVL VVDQSRMVSS PSKVSIRPKM KSVRLLAREA SEARKEIRHN 790 800 810 820 830 840 ARRNKSGNLL RRSSTKLWGS RIVELKPDTT ITSGSVVSQN ATFKWMKGEL IGNGTYGKVF 850 860 870 880 890 900 LAMNINTGEL IAVKQVEIPQ TINGRHDQLR KDIVDSINAE ISMIADLDHL NIVQYLGFEK 910 920 930 940 950 960 TETDISIFLE YVSGGSIGRC LRNYGPFEEQ LVRFVSRQVL YGLSYLHSKG IIHRDLKADN 970 980 990 1000 1010 1020 LLIDFDGVCK ISDFGISKHS DNVYDNDANL SMQGSIFWMA PEVIHNDHQG YSAKVDVWSL 1030 1040 1050 1060 1070 1080 GCVVLEMLAG RRPWSTDEAI QAMFKLGTEK KAPPIPSELV SQVSPEAIQF LNACFTVNAD 1090 1100 1110 VRPTAEELLN HPFMKCDEEF NFKDTNLYDM LCKRKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mkh1, a MEK kinase required for cell wall integrity and proper response to osmotic and temperature stress in Schizosaccharomyces pombe." Sengar A.S., Markley N.A., Marini N.J., Young D. Mol. Cell. Biol. 17:3508-3519(1997) [PubMed: 9199286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SP66.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53872 Genomic DNA. Translation: AAB62319.1. CU329670 Genomic DNA. Translation: CAA94620.1.
PIR	T38073.
RefSeq	NP_593005.1.
3D structure databases
SMR	Q10407. Positions 747-1096.
ModBase	Search...
Protein-protein interaction databases
STRING	Q10407.
Genome annotation databases
GeneID	2542175.
GenomeReviews	Gene locus mkh1 in contig CU329670_GR.
KEGG	spo:SPAC1F3.02c.
NMPDR	fig|4896.1.peg.2975.
Organism-specific databases
GeneDB_Spombe	SPAC1F3.02c.
Phylogenomic databases
eggNOG	fuNOG05291.
OrthoDB	EOG98D2BT.
PhylomeDB	Q10407.
Enzyme and pathway databases
BRENDA	2.7.11.25. 653.
Gene expression databases
ArrayExpress	Q10407.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR015748. MAPKKK3. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22986:SF64. MAPKKK3. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MKH1_SCHPO

Accession

Primary (citable) accession number: Q10407

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)