Q10361 (ETP1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Electron transfer protein 1, mitochondrial

Gene names

Name:	etp1
ORF Names:	SPAC22E12.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Ref.1.
Sequence similarities	Belongs to the COX15/CtaA family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Mitochondrion
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from direct assay. Source: GeneDB_Spombe heme a biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe respiratory chain complex IV assembly Inferred from sequence or structural similarity. Source: GeneDB_Spombe transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to mitochondrial inner membrane Inferred by curator. Source: GeneDB_Spombe
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from direct assay. Source: GeneDB_Spombe metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the CH-CH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 616

Electron transfer protein 1, mitochondrial

PRO_0000006079

Regions

Transmembrane

98 – 118

21

Helical; Potential

Transmembrane

183 – 203

21

Helical; Potential

Transmembrane

213 – 233

21

Helical; Potential

Transmembrane

255 – 275

21

Helical; Potential

Transmembrane

312 – 332

21

Helical; Potential

Transmembrane

381 – 401

21

Helical; Potential

Transmembrane

418 – 438

21

Helical; Potential

Transmembrane

440 – 460

21

Helical; Potential

Domain

502 – 606

105

2Fe-2S ferredoxin-type

Sites

Metal binding

541

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

547

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

550

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

587

1

Iron-sulfur (2Fe-2S) By similarity

Experimental info

Sequence conflict

94

1

P → S no nucleotide entry Ref.1

Sequence conflict

112

1

I → T no nucleotide entry Ref.1

Sequence conflict

443

1

A → V no nucleotide entry Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

616

Helix Strand Turn

Details...

Beta strand

521 – 524

4

Beta strand

530 – 533

4

Beta strand

552 – 554

3

Helix

556 – 561

6

Helix

567 – 573

7

Helix

593 – 595

3

Beta strand

599 – 601

3

Sequences

	Sequence		Length	Mass (Da)
	Q10361 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: D5E3846A28789691	FASTA	616	68,584
	10 20 30 40 50 60 MNISRSSGLM RQFLLQPLRK GCDISCLGRS SWRMSRSFSG SSVLNEINLS RTKNLFLNDC 70 80 90 100 110 120 KFNKNSFEKF FARRLSNSVA PTPGGILQET EKIPSKKVAF WLLGSSALVL AIVVVGGITR 130 140 150 160 170 180 LTESGLSITE WKPITGVIPP LTDEQWNQEF ELYKKSPEFE KLNSHMTVDE FKNIFFWEWF 190 200 210 220 230 240 HRVLGRGIGL TILLPSIYMI VTKRASPWLS KRLIGLTGLV GLQGVIGWWM VKSGLSEELF 250 260 270 280 290 300 SDGSHPRVSH YRLATHLAAA VALYIGLVWT GHGILQRHAF LKSMKSGSTS QLTSMVSSVQ 310 320 330 340 350 360 KMKGFRTSVN SFVGLVLITL LSGAFVAGLD AGMIYCTFPE MGEGRLAPSK SELFDQRFCR 370 380 390 400 410 420 KDDKSDLIWR NMIDNPSLVQ LEHRILAITT FVAACGLFIF SRAKRNILPK KIKTSINVVT 430 440 450 460 470 480 GVVTAQATLG IMTLIYVVPV PLAALHQAGS LVTLTAALSL AQRLHPEYAL KNIRSWTKLI 490 500 510 520 530 540 SSPPKSSISS SILTQQRQFH TFRPSFHSEI KKPLPGTGIK VFFVTPEGRE IMIEGNEEGA 550 560 570 580 590 600 CEGSVACSTC HVIVDPEHYE LLDPPEEDEE DMLDLAFGLE ETSRLGCQVL LRKDLDGIRV 610 RIPAQTRNIR LERPKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional expression of human mitochondrial CYP11B2 in fission yeast and identification of a new internal electron transfer protein, etp1." Bureik M., Schiffler B., Hiraoka Y., Vogel F., Bernhardt R. Biochemistry 41:2311-2321(2002) [PubMed: 11841224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CU329670 Genomic DNA. Translation: CAA93897.2.

PIR

T38167.

RefSeq

NP_594836.2. NM_001020265.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WLB	X-ray	2.60	A/B	516-603	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q10361.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

SPAC22E12.10c.1; SPAC22E12.10c.1:pep; SPAC22E12.10c.

GeneID

2541839.

GenomeReviews

Gene locus etp1 in contig CU329670_GR.

KEGG

spo:SPAC22E12.10c.

NMPDR

fig|4896.1.peg.4806.

Organism-specific databases

GeneDB_Spombe

SPAC22E12.10c.

Phylogenomic databases

eggNOG

fuNOG04581.

GeneTree

EFGT00050000004064.

HOGENOM

HBG628966.

OrthoDB

EOG4MWCFV.

Enzyme and pathway databases

BioCyc

SPOM-XXX-01:SPOM-XXX-01-002908-MONOMER.

Gene expression databases

ArrayExpress

Q10361.

Family and domain databases

InterPro

IPR001055. Adrenodoxin.
IPR018298. Adrenodoxin_Fe-S_BS.
IPR012675. Beta-grasp_ferredoxin-type.
IPR001041. Ferredoxin.
IPR003780. HemeA_syn.
IPR023754. HemeA_Synthase_type2.
[Graphical view]

Gene3D

G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.

KO

K02259.

Pfam

PF02628. COX15-CtaA. 1 hit.
PF00111. Fer2. 1 hit.
[Graphical view]

PRINTS

PR00355. ADRENODOXIN.

SUPFAM

SSF54292. Ferredoxin. 1 hit.

PROSITE

PS51085. 2FE2S_FER_2. False negative.
PS00814. ADX. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ETP1_SCHPO

Accession

Primary (citable) accession number: Q10361

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 22, 2009
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program