Q10359 (GMH3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,2-galactosyltransferase gmh3
EC=2.4.1.-

Gene names

Name:	gmh3
ORF Names:	SPAC22E12.06c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the galactosylation of the N-linked core oligosaccharide Man9GlcNAc2. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall biogenesis Traceable author statement Ref.1. Source: PomBase protein N-linked glycosylation Inferred from mutant phenotype Ref.1. Source: PomBase protein galactosylation Traceable author statement Ref.1. Source: PomBase
Cellular_component	Golgi membrane Inferred from sequence or structural similarity. Source: PomBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-1,2-galactosyltransferase activity Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Alpha-1,2-galactosyltransferase gmh3		PRO_0000215168
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 332	301	Lumenal Potential
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10359 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 998E687D846BCEEE	FASTA	332	38,054
	10 20 30 40 50 60 MAVLQWKLSR VIFVLLIIGI SSFLIYFQFG NSFSASPALE RLQDVFSKPS VQEYDYLVPL 70 80 90 100 110 120 DEDMQGLSTT YHQATINEDD PQEPKSHEIV ILLASDGRSS GNMAPETFNQ CIENRINYAK 130 140 150 160 170 180 HHNYGFEYVN VSQMNIPPVW AKMPAIIQTM NKHPHAKWIW WLDQDALILN TELSIQEHIL 190 200 210 220 230 240 SPDVLVEKLM KNEPMISPFS ADLERLTPSS YTVDSARSLG LLISQDLNGL NAGSFFVRRS 250 260 270 280 290 300 PMMALFLDLW GDKSFRENKV ADHEQALLGY FVRYHPEIAA IIGILPQTLI NSYPVGQPEM 310 320 330 GWKEGHLVIH FAGCWVENRC QVLWDEYRER VH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe." Yoko-o T., Roy S.K., Jigami Y. Eur. J. Biochem. 257:630-637(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA93893.1.
PIR	T38163.
RefSeq	NP_594832.1. NM_001020261.2.
3D structure databases
ProteinModelPortal	Q10359.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC22E12.06c-1.
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC22E12.06c.1; SPAC22E12.06c.1:pep; SPAC22E12.06c.
GeneID	2541748.
KEGG	spo:SPAC22E12.06c.
Organism-specific databases
PomBase	SPAC22E12.06c.
Phylogenomic databases
eggNOG	NOG246716.
HOGENOM	HOG000112745.
OrthoDB	EOG49PF7F.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802839.

Entry information

Entry name

GMH3_SCHPO

Accession

Primary (citable) accession number: Q10359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program