Q10359 (GMH3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,2-galactosyltransferase gmh3
EC=2.4.1.-

Gene names

Name:	gmh3
ORF Names:	SPAC22E12.06c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the galactosylation of the N-linked core oligosaccharide Man9GlcNAc2. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall biogenesis Traceable author statement. Source: GeneDB_Spombe protein N-linked glycosylation Inferred from mutant phenotype. Source: GeneDB_Spombe protein galactosylation Traceable author statement. Source: GeneDB_Spombe
Cellular component	Golgi membrane Inferred from sequence or structural similarity. Source: GeneDB_Spombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,2-galactosyltransferase activity Inferred from mutant phenotype. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Alpha-1,2-galactosyltransferase gmh3		PRO_0000215168
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 332	301	Lumenal Potential
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10359 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 998E687D846BCEEE	FASTA	332	38,054
	10 20 30 40 50 60 MAVLQWKLSR VIFVLLIIGI SSFLIYFQFG NSFSASPALE RLQDVFSKPS VQEYDYLVPL 70 80 90 100 110 120 DEDMQGLSTT YHQATINEDD PQEPKSHEIV ILLASDGRSS GNMAPETFNQ CIENRINYAK 130 140 150 160 170 180 HHNYGFEYVN VSQMNIPPVW AKMPAIIQTM NKHPHAKWIW WLDQDALILN TELSIQEHIL 190 200 210 220 230 240 SPDVLVEKLM KNEPMISPFS ADLERLTPSS YTVDSARSLG LLISQDLNGL NAGSFFVRRS 250 260 270 280 290 300 PMMALFLDLW GDKSFRENKV ADHEQALLGY FVRYHPEIAA IIGILPQTLI NSYPVGQPEM 310 320 330 GWKEGHLVIH FAGCWVENRC QVLWDEYRER VH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe." Yoko-o T., Roy S.K., Jigami Y. Eur. J. Biochem. 257:630-637(1998) [PubMed: 9839953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA93893.1.
PIR	T38163.
RefSeq	NP_594832.1. NM_001020261.1.
3D structure databases
ProteinModelPortal	Q10359.
ModBase	Search...
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC22E12.06c.1; SPAC22E12.06c.1:pep; SPAC22E12.06c.
GeneID	2541748.
GenomeReviews	Gene locus gmh3 in contig CU329670_GR.
KEGG	spo:SPAC22E12.06c.
NMPDR	fig|4896.1.peg.4802.
Organism-specific databases
GeneDB_Spombe	SPAC22E12.06c.
Phylogenomic databases
eggNOG	fuNOG08674.
GeneTree	EFGT00050000004280.
HOGENOM	HBG207378.
OrthoDB	EOG49PF7F.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002904-MONOMER.
Gene expression databases
ArrayExpress	Q10359.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMH3_SCHPO

Accession

Primary (citable) accession number: Q10359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program