Reviewed, UniProtKB/Swiss-Prot Q10359 (GMH3_SCHPO)

Names and origin

Protein names

Recommended name:
    Alpha-1,2-galactosyltransferase gmh3
    EC=2.4.1.-

Gene names

Name:	gmh3
ORF Names:	SPAC22E12.06c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the galactosylation of the N-linked core oligosaccharide Man9GlcNAc2. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall biogenesis Ref.1 Traceable author statement. Source: GeneDB_SPombe protein amino acid N-linked glycosylation Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid galactosylation Ref.1 Traceable author statement. Source: GeneDB_SPombe
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,2-galactosyltransferase activity Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Alpha-1,2-galactosyltransferase gmh3		PRO_0000215168
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 332	301	Lumenal Potential
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10359-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 998E687D846BCEEE	FASTA	332	38,054
	10 20 30 40 50 60 MAVLQWKLSR VIFVLLIIGI SSFLIYFQFG NSFSASPALE RLQDVFSKPS VQEYDYLVPL 70 80 90 100 110 120 DEDMQGLSTT YHQATINEDD PQEPKSHEIV ILLASDGRSS GNMAPETFNQ CIENRINYAK 130 140 150 160 170 180 HHNYGFEYVN VSQMNIPPVW AKMPAIIQTM NKHPHAKWIW WLDQDALILN TELSIQEHIL 190 200 210 220 230 240 SPDVLVEKLM KNEPMISPFS ADLERLTPSS YTVDSARSLG LLISQDLNGL NAGSFFVRRS 250 260 270 280 290 300 PMMALFLDLW GDKSFRENKV ADHEQALLGY FVRYHPEIAA IIGILPQTLI NSYPVGQPEM 310 320 330 GWKEGHLVIH FAGCWVENRC QVLWDEYRER VH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe." Yoko-o T., Roy S.K., Jigami Y. Eur. J. Biochem. 257:630-637(1998) [PubMed: 9839953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA93893.1.
PIR	T38163.
RefSeq	NP_594832.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Genome annotation databases
GeneID	2541748.
KEGG	spo:SPAC22E12.06c.
NMPDR	fig|4896.1.peg.4802.
Organism-specific databases
GeneDB_Spombe	SPAC22E12.06c.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002904-MON.
Gene expression databases
ArrayExpress	Q10359.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMH3_SCHPO

Accession

Primary (citable) accession number: Q10359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)