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Protein

Glutathione-independent glyoxalase DJ-1

Gene

hsp3106

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step (PubMed:24758716). May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.2 Publications

Kineticsi

kcat is 85.7 min(-1) with methylglyoxal as substrate.1 Publication

  1. KM=10.8 mM for methylglyoxal1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei16 – 1611 Publication
    Active sitei111 – 11111 Publication
    Active sitei130 – 13011 Publication

    GO - Molecular functioni

    • glyoxalase III activity Source: PomBase

    GO - Biological processi

    • lactate biosynthetic process Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Stress response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase DJ-11 Publication (EC:4.2.1.1302 Publications)
    Alternative name(s):
    Heat shock protein 31 homolog 6By similarity
    Protein DJ-1 homolog
    Gene namesi
    Name:hsp3106Imported
    Synonyms:spDJ-11 Publication
    ORF Names:SPAC22E12.03cImported
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC22E12.03c.
    PomBaseiSPAC22E12.03c. hsp3106.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: PomBase
    • cytosol Source: PomBase
    • nucleus Source: PomBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi16 – 161E → A: Nearly completely abolishes enzymatic activity. 1 Publication
    Mutagenesisi111 – 1111C → A: Nearly completely abolishes enzymatic activity. 1 Publication
    Mutagenesisi130 – 1301H → A: Leads to 5- to 6-fold reduction in catalytic efficiency. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 191191Glutathione-independent glyoxalase DJ-1PRO_0000157857Add
    BLAST

    Proteomic databases

    MaxQBiQ10356.

    Interactioni

    Protein-protein interaction databases

    BioGridi278266. 1 interaction.
    MINTiMINT-4699059.

    Structurei

    Secondary structure

    1
    191
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96Combined sources
    Helixi14 – 2613Combined sources
    Beta strandi31 – 388Combined sources
    Beta strandi43 – 453Combined sources
    Beta strandi51 – 533Combined sources
    Beta strandi55 – 573Combined sources
    Helixi58 – 603Combined sources
    Helixi64 – 707Combined sources
    Beta strandi72 – 765Combined sources
    Helixi80 – 878Combined sources
    Helixi90 – 9910Combined sources
    Beta strandi106 – 1105Combined sources
    Helixi111 – 1144Combined sources
    Helixi115 – 1195Combined sources
    Beta strandi125 – 1273Combined sources
    Helixi131 – 1333Combined sources
    Helixi134 – 1396Combined sources
    Beta strandi147 – 1526Combined sources
    Beta strandi155 – 1584Combined sources
    Helixi161 – 1633Combined sources
    Helixi164 – 17512Combined sources
    Helixi178 – 18710Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GE0X-ray1.45A/B/C/D1-191[»]
    4GE3X-ray1.50A/B/C/D1-191[»]
    4QYTX-ray1.05A/B/C/D1-191[»]
    ProteinModelPortaliQ10356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C56 family.Curated

    Phylogenomic databases

    eggNOGiCOG0693.
    HOGENOMiHOG000063194.
    InParanoidiQ10356.
    OMAiWIGMICA.
    OrthoDBiEOG7QNVZ1.
    PhylomeDBiQ10356.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. ThiJ/PfpI.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q10356-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVKVCLFVAD GTDEIEFSAP WGIFKRAEIP IDSVYVGENK DRLVKMSRDV
    60 70 80 90 100
    EMYANRSYKE IPSADDFAKQ YDIAIIPGGG LGAKTLSTTP FVQQVVKEFY
    110 120 130 140 150
    KKPNKWIGMI CAGTLTAKTS GLPNKQITGH PSVRGQLEEG GYKYLDQPVV
    160 170 180 190
    LEENLITSQG PGTAMLFGLK LLEQVASKDK YNAVYKSLSM P
    Length:191
    Mass (Da):21,078
    Last modified:October 1, 1996 - v1
    Checksum:i909817B8D554AB2E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93890.1.
    PIRiT38160.
    RefSeqiNP_594829.1. NM_001020258.2.

    Genome annotation databases

    EnsemblFungiiSPAC22E12.03c.1; SPAC22E12.03c.1:pep; SPAC22E12.03c.
    GeneIDi2541772.
    KEGGispo:SPAC22E12.03c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93890.1.
    PIRiT38160.
    RefSeqiNP_594829.1. NM_001020258.2.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GE0X-ray1.45A/B/C/D1-191[»]
    4GE3X-ray1.50A/B/C/D1-191[»]
    4QYTX-ray1.05A/B/C/D1-191[»]
    ProteinModelPortaliQ10356.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi278266. 1 interaction.
    MINTiMINT-4699059.

    Proteomic databases

    MaxQBiQ10356.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPAC22E12.03c.1; SPAC22E12.03c.1:pep; SPAC22E12.03c.
    GeneIDi2541772.
    KEGGispo:SPAC22E12.03c.

    Organism-specific databases

    EuPathDBiFungiDB:SPAC22E12.03c.
    PomBaseiSPAC22E12.03c. hsp3106.

    Phylogenomic databases

    eggNOGiCOG0693.
    HOGENOMiHOG000063194.
    InParanoidiQ10356.
    OMAiWIGMICA.
    OrthoDBiEOG7QNVZ1.
    PhylomeDBiQ10356.

    Miscellaneous databases

    NextBioi20802863.
    PROiQ10356.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. ThiJ/PfpI.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    3. "Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe."
      Zhao Q., Su Y., Wang Z., Chen C., Wu T., Huang Y.
      BMC Evol. Biol. 14:86-86(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-16; CYS-111 AND HIS-130.
    4. "A glutathione-independent glyoxalase of the DJ-1 superfamily plays an important role in managing metabolically generated methylglyoxal in Candida albicans."
      Hasim S., Hussin N.A., Alomar F., Bidasee K.R., Nickerson K.W., Wilson M.A.
      J. Biol. Chem. 289:1662-1674(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    5. "Influence of peptide dipoles and hydrogen bonds on reactive cysteine pKa values in fission yeast DJ-1."
      Madzelan P., Labunska T., Wilson M.A.
      FEBS J. 279:4111-4120(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS).

    Entry informationi

    Entry nameiDJ1_SCHPO
    AccessioniPrimary (citable) accession number: Q10356
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: July 22, 2015
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.