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Protein

Glutathione-independent glyoxalase DJ-1

Gene

hsp3106

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step (PubMed:24758716). May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.2 Publications

Kineticsi

kcat is 85.7 min(-1) with methylglyoxal as substrate.1 Publication
  1. KM=10.8 mM for methylglyoxal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei161 Publication1
    Active sitei1111 Publication1
    Active sitei1301 Publication1

    GO - Molecular functioni

    • glyoxalase III activity Source: PomBase

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    Biological processStress response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase DJ-11 Publication (EC:4.2.1.1302 Publications)
    Alternative name(s):
    Heat shock protein 31 homolog 6By similarity
    Protein DJ-1 homolog
    Gene namesi
    Name:hsp3106Imported
    Synonyms:spDJ-11 Publication
    ORF Names:SPAC22E12.03cImported
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC22E12.03c.
    PomBaseiSPAC22E12.03c. hsp3106.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: PomBase
    • cytosol Source: PomBase
    • nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi16E → A: Nearly completely abolishes enzymatic activity. 1 Publication1
    Mutagenesisi111C → A: Nearly completely abolishes enzymatic activity. 1 Publication1
    Mutagenesisi130H → A: Leads to 5- to 6-fold reduction in catalytic efficiency. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001578571 – 191Glutathione-independent glyoxalase DJ-1Add BLAST191

    Proteomic databases

    MaxQBiQ10356.
    PRIDEiQ10356.

    Interactioni

    Protein-protein interaction databases

    BioGridi278266. 144 interactors.
    MINTiMINT-4699059.

    Structurei

    Secondary structure

    1191
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 9Combined sources6
    Helixi14 – 26Combined sources13
    Beta strandi31 – 38Combined sources8
    Beta strandi43 – 45Combined sources3
    Beta strandi51 – 53Combined sources3
    Beta strandi55 – 57Combined sources3
    Helixi58 – 60Combined sources3
    Helixi64 – 70Combined sources7
    Beta strandi72 – 76Combined sources5
    Helixi80 – 87Combined sources8
    Helixi90 – 99Combined sources10
    Beta strandi106 – 110Combined sources5
    Helixi111 – 114Combined sources4
    Helixi115 – 119Combined sources5
    Beta strandi125 – 127Combined sources3
    Helixi131 – 133Combined sources3
    Helixi134 – 139Combined sources6
    Beta strandi147 – 152Combined sources6
    Beta strandi155 – 158Combined sources4
    Helixi161 – 163Combined sources3
    Helixi164 – 175Combined sources12
    Helixi178 – 187Combined sources10

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4GE0X-ray1.45A/B/C/D1-191[»]
    4GE3X-ray1.50A/B/C/D1-191[»]
    4QYTX-ray1.05A/B/C/D1-191[»]
    ProteinModelPortaliQ10356.
    SMRiQ10356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C56 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000063194.
    InParanoidiQ10356.
    OMAiWIGMICA.
    OrthoDBiEOG092C4PPS.
    PhylomeDBiQ10356.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    PfamiView protein in Pfam
    PF01965. DJ-1_PfpI. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q10356-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVKVCLFVAD GTDEIEFSAP WGIFKRAEIP IDSVYVGENK DRLVKMSRDV
    60 70 80 90 100
    EMYANRSYKE IPSADDFAKQ YDIAIIPGGG LGAKTLSTTP FVQQVVKEFY
    110 120 130 140 150
    KKPNKWIGMI CAGTLTAKTS GLPNKQITGH PSVRGQLEEG GYKYLDQPVV
    160 170 180 190
    LEENLITSQG PGTAMLFGLK LLEQVASKDK YNAVYKSLSM P
    Length:191
    Mass (Da):21,078
    Last modified:October 1, 1996 - v1
    Checksum:i909817B8D554AB2E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93890.1.
    PIRiT38160.
    RefSeqiNP_594829.1. NM_001020258.2.

    Genome annotation databases

    EnsemblFungiiSPAC22E12.03c.1; SPAC22E12.03c.1:pep; SPAC22E12.03c.
    GeneIDi2541772.
    KEGGispo:SPAC22E12.03c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93890.1.
    PIRiT38160.
    RefSeqiNP_594829.1. NM_001020258.2.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4GE0X-ray1.45A/B/C/D1-191[»]
    4GE3X-ray1.50A/B/C/D1-191[»]
    4QYTX-ray1.05A/B/C/D1-191[»]
    ProteinModelPortaliQ10356.
    SMRiQ10356.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi278266. 144 interactors.
    MINTiMINT-4699059.

    Proteomic databases

    MaxQBiQ10356.
    PRIDEiQ10356.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPAC22E12.03c.1; SPAC22E12.03c.1:pep; SPAC22E12.03c.
    GeneIDi2541772.
    KEGGispo:SPAC22E12.03c.

    Organism-specific databases

    EuPathDBiFungiDB:SPAC22E12.03c.
    PomBaseiSPAC22E12.03c. hsp3106.

    Phylogenomic databases

    HOGENOMiHOG000063194.
    InParanoidiQ10356.
    OMAiWIGMICA.
    OrthoDBiEOG092C4PPS.
    PhylomeDBiQ10356.

    Miscellaneous databases

    PROiPR:Q10356.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    PfamiView protein in Pfam
    PF01965. DJ-1_PfpI. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDJ1_SCHPO
    AccessioniPrimary (citable) accession number: Q10356
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: March 15, 2017
    This is version 102 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.