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Protein

Glutathione-independent glyoxalase DJ-1

Gene

hsp3106

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step (PubMed:24758716). May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.2 Publications

Kineticsi

kcat is 85.7 min(-1) with methylglyoxal as substrate.1 Publication

  1. KM=10.8 mM for methylglyoxal1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 1611 Publication
Active sitei111 – 11111 Publication
Active sitei130 – 13011 Publication

GO - Molecular functioni

  1. glyoxalase III activity Source: PomBase

GO - Biological processi

  1. lactate biosynthetic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-independent glyoxalase DJ-11 Publication (EC:4.2.1.1302 Publications)
Alternative name(s):
Heat shock protein 31 homolog 6By similarity
Protein DJ-1 homolog
Gene namesi
Name:hsp3106Imported
Synonyms:spDJ-11 Publication
ORF Names:SPAC22E12.03cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

PomBaseiSPAC22E12.03c.

Subcellular locationi

Cytoplasm 2 Publications. Nucleus 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi16 – 161E → A: Nearly completely abolishes enzymatic activity. 1 Publication
Mutagenesisi111 – 1111C → A: Nearly completely abolishes enzymatic activity. 1 Publication
Mutagenesisi130 – 1301H → A: Leads to 5- to 6-fold reduction in catalytic efficiency. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Glutathione-independent glyoxalase DJ-1PRO_0000157857Add
BLAST

Proteomic databases

MaxQBiQ10356.

Interactioni

Protein-protein interaction databases

BioGridi278266. 1 interaction.
MINTiMINT-4699059.
STRINGi4896.SPAC22E12.03c-1.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi14 – 2613Combined sources
Beta strandi31 – 388Combined sources
Beta strandi43 – 453Combined sources
Beta strandi51 – 533Combined sources
Beta strandi55 – 573Combined sources
Helixi58 – 603Combined sources
Helixi64 – 707Combined sources
Beta strandi72 – 765Combined sources
Helixi80 – 878Combined sources
Helixi90 – 9910Combined sources
Beta strandi106 – 1105Combined sources
Helixi111 – 1144Combined sources
Helixi115 – 1195Combined sources
Beta strandi125 – 1273Combined sources
Helixi131 – 1333Combined sources
Helixi134 – 1396Combined sources
Beta strandi147 – 1526Combined sources
Beta strandi155 – 1584Combined sources
Helixi161 – 1633Combined sources
Helixi164 – 17512Combined sources
Helixi178 – 18710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GE0X-ray1.45A/B/C/D1-191[»]
4GE3X-ray1.50A/B/C/D1-191[»]
4QYTX-ray1.05A/B/C/D1-191[»]
ProteinModelPortaliQ10356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C56 family.Curated

Phylogenomic databases

eggNOGiCOG0693.
HOGENOMiHOG000063194.
InParanoidiQ10356.
OMAiPRVANEW.
OrthoDBiEOG7QNVZ1.
PhylomeDBiQ10356.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002818. ThiJ/PfpI.
[Graphical view]
PfamiPF01965. DJ-1_PfpI. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVCLFVAD GTDEIEFSAP WGIFKRAEIP IDSVYVGENK DRLVKMSRDV
60 70 80 90 100
EMYANRSYKE IPSADDFAKQ YDIAIIPGGG LGAKTLSTTP FVQQVVKEFY
110 120 130 140 150
KKPNKWIGMI CAGTLTAKTS GLPNKQITGH PSVRGQLEEG GYKYLDQPVV
160 170 180 190
LEENLITSQG PGTAMLFGLK LLEQVASKDK YNAVYKSLSM P
Length:191
Mass (Da):21,078
Last modified:September 30, 1996 - v1
Checksum:i909817B8D554AB2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93890.1.
PIRiT38160.
RefSeqiNP_594829.1. NM_001020258.2.

Genome annotation databases

EnsemblFungiiSPAC22E12.03c.1; SPAC22E12.03c.1:pep; SPAC22E12.03c.
GeneIDi2541772.
KEGGispo:SPAC22E12.03c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93890.1.
PIRiT38160.
RefSeqiNP_594829.1. NM_001020258.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GE0X-ray1.45A/B/C/D1-191[»]
4GE3X-ray1.50A/B/C/D1-191[»]
4QYTX-ray1.05A/B/C/D1-191[»]
ProteinModelPortaliQ10356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278266. 1 interaction.
MINTiMINT-4699059.
STRINGi4896.SPAC22E12.03c-1.

Proteomic databases

MaxQBiQ10356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC22E12.03c.1; SPAC22E12.03c.1:pep; SPAC22E12.03c.
GeneIDi2541772.
KEGGispo:SPAC22E12.03c.

Organism-specific databases

PomBaseiSPAC22E12.03c.

Phylogenomic databases

eggNOGiCOG0693.
HOGENOMiHOG000063194.
InParanoidiQ10356.
OMAiPRVANEW.
OrthoDBiEOG7QNVZ1.
PhylomeDBiQ10356.

Miscellaneous databases

NextBioi20802863.
PROiQ10356.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002818. ThiJ/PfpI.
[Graphical view]
PfamiPF01965. DJ-1_PfpI. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. "Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe."
    Zhao Q., Su Y., Wang Z., Chen C., Wu T., Huang Y.
    BMC Evol. Biol. 14:86-86(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-16; CYS-111 AND HIS-130.
  4. "A glutathione-independent glyoxalase of the DJ-1 superfamily plays an important role in managing metabolically generated methylglyoxal in Candida albicans."
    Hasim S., Hussin N.A., Alomar F., Bidasee K.R., Nickerson K.W., Wilson M.A.
    J. Biol. Chem. 289:1662-1674(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  5. "Influence of peptide dipoles and hydrogen bonds on reactive cysteine pKa values in fission yeast DJ-1."
    Madzelan P., Labunska T., Wilson M.A.
    FEBS J. 279:4111-4120(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS).

Entry informationi

Entry nameiDJ1_SCHPO
AccessioniPrimary (citable) accession number: Q10356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: March 3, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.