Q10334 (ALAT_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative alanine aminotransferase
EC=2.6.1.2

Alternative name(s):
Glutamate pyruvate transaminase
Short name=GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase

Gene names

ORF Names:

SPBC582.08

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-alanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway L-alanine metabolic process Inferred from sequence or structural similarity. Source: PomBase glutamate biosynthetic process Inferred by curator. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase mitochondrion Inferred from sequence or structural similarity. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	L-alanine:2-oxoglutarate aminotransferase activity Inferred from sequence or structural similarity. Source: PomBase pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Putative alanine aminotransferase		PRO_0000123938
Amino acid modifications
	Modified residue	326	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10334 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6F6A0756A0458FAC	FASTA	505	56,628
	10 20 30 40 50 60 MFTDYPNDIN CESPRMSDLD GFCQNAFSDL NSLNQQVFKA NYAVRGALAI LADEIQDDLL 70 80 90 100 110 120 ENPSSYPFSE IVYANIGNPQ QMGQSPITFV RQVLSLCQYP TLLDHAEEKW FQNLFPTDVV 130 140 150 160 170 180 QRSKMLLKES GSLGAYSASQ GIPLVRRHVA DFIRARDGFD CEPSDIYLTS GASHAARLIM 190 200 210 220 230 240 TLIIARPTDG VMVPAPQYPL YGAQIDLMSG SMVSYSLSEE NNWDIDFDQF KKSFDEASKK 250 260 270 280 290 300 GINVRLCVVI NPGNPTGACI SENSMEKVLR FAKAKGIVLL ADEVYQNNIY QNKFHSFRRK 310 320 330 340 350 360 LGELREKEPD NHWDQVSLIS VNSVSKGQFG ECGQRGGYLD VVNIPEPAKD QILKLATIDI 370 380 390 400 410 420 CPPVAGQLLV DMLVNPPKPG DPSYDLFIKE VDEIHEALRL QCRQLYEGTK RMKRVSCLEP 430 440 450 460 470 480 HGAMYLHPSV SLPEKLITTA KAQKIQPDEF YAIELLKRSG ICVVPGSGFG QPEGDYHIRI 490 500 TFLAKGTEYI ERFVKAHNEI MDLYE

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB46671.1.
PIR	T37975.
RefSeq	NP_595176.1. NM_001021084.2.
3D structure databases
ProteinModelPortal	Q10334.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4698867.
STRING	4896.SPBC582.08-1.
Proteomic databases
PaxDb	Q10334.
PRIDE	Q10334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC582.08.1; SPBC582.08.1:pep; SPBC582.08.
GeneID	2540891.
KEGG	spo:SPBC582.08.
Organism-specific databases
PomBase	SPBC582.08.
Phylogenomic databases
eggNOG	COG0436.
HOGENOM	HOG000215020.
KO	K00814.
OMA	CIEEVLH.
OrthoDB	EOG41VPB9.
Enzyme and pathway databases
UniPathway	UPA00528; UER00586.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...
Other
NextBio	20802006.

Entry information

Entry name

ALAT_SCHPO

Accession

Primary (citable) accession number: Q10334

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program