Q10332 (YBMA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized ATP-dependent helicase C582.10c
EC=3.6.4.-

Gene names

ORF Names:

SPBC582.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus Ref.2.
Sequence similarities	Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleotide-excision repair Inferred from sequence orthology. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase nucleotide-excision repair factor 4 complex Inferred from sequence orthology. Source: PomBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from sequence orthology. Source: PomBase helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 830	830	Uncharacterized ATP-dependent helicase C582.10c		PRO_0000074382
Regions
	Domain	249 – 433	185	Helicase ATP-binding
	Domain	662 – 816	155	Helicase C-terminal
	Nucleotide binding	262 – 269	8	ATP Potential
	Motif	384 – 387	4	DEAH box
Amino acid modifications
	Modified residue	712	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q10332 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 47086968FDD951CB	FASTA	830	93,945
	10 20 30 40 50 60 MEKASKNKES GVKKANNSFL QNFGVNTAGK ENESTSLPRL PKSEDESIPK QSIKSKNKKK 70 80 90 100 110 120 TQHFLSDAFE SLKRQEIDTN EKEVIVSAPI TSKPAQVFYS KKLERKDEGI RKWEKDPFAP 130 140 150 160 170 180 ISVKSGAWKK PYTKVPEVSI NKATSKRTDL INDKPIVIPI PRASTSTLYF GKHNKPTSEN 190 200 210 220 230 240 RKGPIGIPTE EILTSQNTQA MLHKLFENNV LDNVKDDSMQ RQSSFIPGMH IRLLDHQVQG 250 260 270 280 290 300 LTWLKSRETV SKSSASGGIL ADDMGLGKTI QMIALILSHP LPKKKHSIKS TLVVAPLSLI 310 320 330 340 350 360 KQWESEVQTK SKLTAIVYHG ASRYKLLKVI HEYDVVITTY QILVSEWVSH NTTGTDGKSP 370 380 390 400 410 420 TEAKSYEKKK PSLFAFYWWR IILDEAHTIK NKSSKSALAC CALQGINRWC LTGTPLQNNV 430 440 450 460 470 480 DELYSLVKFL HINPFNDQSV WKDQISLPLC QGEENLVFKR LRMLLSVIML RRTKTLLEAN 490 500 510 520 530 540 AGKDGTGGAL KLSKRLVYKV ICKFEESERD FYSNLARNME RTMSNFVNSG KLGKNYTNIL 550 560 570 580 590 600 CLLLRLRQAC NHPQSLNFQF EQDVDAFNAL DGAANTNKLA SDQDVDDLAN LLETVEIGSR 610 620 630 640 650 660 KKSFCTICMA ELPPDFHEKK CKDCSRNFKE LDKGIQDPND KTLYKSSKIR EILKILSLDE 670 680 690 700 710 720 QEEDDTVRGL RKTIIFSQFT TFLDIIDLHL RKAGIGFVRY DGRMNNRARE KSLDLLRSDS 730 740 750 760 770 780 GTQVLLCSLK CGALGLNLTC ASRVILCDVW WNPAIEEQAI DRVHRIGQRR DVLVYKLVVE 790 800 810 820 830 NTIEEKIVEL QNLKRDLAKQ ALGDGKKSVF TSKKLTLNDL LFLFNKRAAA

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 264-443, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-712, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB46673.1. AB027965 Genomic DNA. Translation: BAA87269.1.
PIR	T37973.
RefSeq	NP_595178.1. NM_001021086.2.
3D structure databases
ProteinModelPortal	Q10332.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC582.10c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC582.10c.1; SPBC582.10c.1:pep; SPBC582.10c.
GeneID	2540907.
KEGG	spo:SPBC582.10c.
Organism-specific databases
PomBase	SPBC582.10c.
Phylogenomic databases
eggNOG	COG0553.
HOGENOM	HOG000241058.
OMA	QACNHPQ.
OrthoDB	EOG44XNQX.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802022.

Entry information

Entry name

YBMA_SCHPO

Accession

Primary (citable) accession number: Q10332

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program