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Q10325

- MST2_SCHPO

UniProt

Q10325 - MST2_SCHPO

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Protein

Histone acetyltransferase mst2

Gene
mst2, SPAC17G8.13c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the mst2 complex which is a highly specific H3 lysine 14 (H3K14) acetyltransferase that functions together with gcn5 to regulate global levels of H3K14 acetylation (H3K14ac), critical for DNA damage checkpoint activation. Negatively regulates telomere silencing. Telomere silencing is increased due to histone hypoacetylation and/or an increase in the ratio of methylated histones to acetylated histones. Telomeric histone acetylation contributes to normal meiotic progression.2 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981 By similarity
Active sitei240 – 2401Nucleophile By similarity
Binding sitei243 – 2431Acetyl-CoA By similarity
Binding sitei278 – 2781Acetyl-CoA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri132 – 15423C2HC-typeAdd
BLAST

GO - Molecular functioni

  1. histone acetyltransferase activity (H3-K14 specific) Source: PomBase
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB-KW
  2. heterochromatin maintenance involved in chromatin silencing at centromere outer repeat region Source: PomBase
  3. histone H3-K14 acetylation Source: GOC
  4. negative regulation of chromatin silencing at telomere Source: PomBase
  5. regulation of meiotic cell cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Transferase

Keywords - Biological processi

DNA damage

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase mst2 (EC:2.3.1.48)
Gene namesi
Name:mst2
ORF Names:SPAC17G8.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC17G8.13c.

Subcellular locationi

Cytoplasm. Nucleus 2 Publications

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. histone acetyltransferase complex Source: PomBase
  3. Mst2 histone acetyltransferase complex Source: PomBase
  4. nuclear chromatin Source: PomBase
  5. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Histone acetyltransferase mst2PRO_0000051565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981N6-acetyllysine; by autocatalysis By similarity

Post-translational modificationi

Autoacetylation at Lys-198 is required for proper function By similarity.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ10325.

Interactioni

Subunit structurei

Component of the mst2 complex composed of at least eaf6, mst2, nto1, pdp3, ptf1, ptf2 and tfg3.1 Publication

Protein-protein interaction databases

BioGridi278645. 96 interactions.
MINTiMINT-4698771.
STRINGi4896.SPAC17G8.13c-1.

Structurei

3D structure databases

ProteinModelPortaliQ10325.
SMRiQ10325. Positions 102-372.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni248 – 2547Acetyl-CoA binding By similarity

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri132 – 15423C2HC-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5027.
KOiK11378.
OMAiYICESCL.
OrthoDBiEOG7RFTRR.
PhylomeDBiQ10325.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR002717. MOZ_SAS.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10325-1 [UniParc]FASTAAdd to Basket

« Hide

MPATILKSTA SSKTLLIIKF DTNSSRYPFY KKHLLELNSE STFLGLLTKG    50
QADTSITRLN QDDVRLFEKA KTVADRTKDV AYSFSDPILS TQLRTPPPQP 100
TSIRYLYFGT YRIKPWYTSP YPEEYSCAKN LYICESCLKY MNSDHVLQRH 150
KMKCSWSYPP GDEIYRDKNI SIFEVDGQRQ PIYCQNLCLL AKMFLHSKML 200
YYDVEPFLFY VLTEFDGQEC KVIGYFSKEK RSASDYNVSC ILTLPIYQRR 250
GYGVFLIDFS YLLTQVEGKL GSPEKPLSDL GLVTYRSYWK MRVAKALLEI 300
TTPISINAIA KSTSMVCDDV ISTLESLSVF KYDPLKKKYV LQLKRDELEN 350
VYKAWNIKHP QRVNPKLLRW TPYLGEEQIS NLLLKENILI PLPQKRLLDN 400
SHHLDSV 407
Length:407
Mass (Da):47,275
Last modified:October 1, 1996 - v1
Checksum:i27B70EB7AB947102
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA93696.1.
PIRiT37865.
RefSeqiNP_593736.1. NM_001019167.2.

Genome annotation databases

EnsemblFungiiSPAC17G8.13c.1; SPAC17G8.13c.1:pep; SPAC17G8.13c.
GeneIDi2542170.
KEGGispo:SPAC17G8.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA93696.1 .
PIRi T37865.
RefSeqi NP_593736.1. NM_001019167.2.

3D structure databases

ProteinModelPortali Q10325.
SMRi Q10325. Positions 102-372.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 278645. 96 interactions.
MINTi MINT-4698771.
STRINGi 4896.SPAC17G8.13c-1.

Proteomic databases

MaxQBi Q10325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC17G8.13c.1 ; SPAC17G8.13c.1:pep ; SPAC17G8.13c .
GeneIDi 2542170.
KEGGi spo:SPAC17G8.13c.

Organism-specific databases

PomBasei SPAC17G8.13c.

Phylogenomic databases

eggNOGi COG5027.
KOi K11378.
OMAi YICESCL.
OrthoDBi EOG7RFTRR.
PhylomeDBi Q10325.

Miscellaneous databases

NextBioi 20803241.

Family and domain databases

Gene3Di 3.40.630.30. 1 hit.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR002717. MOZ_SAS.
[Graphical view ]
Pfami PF01853. MOZ_SAS. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing."
    Gomez E.B., Espinosa J.M., Forsburg S.L.
    Mol. Cell. Biol. 25:8887-8903(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  3. "Histone H3 lysine 14 acetylation is required for activation of a DNA damage checkpoint in fission yeast."
    Wang Y., Kallgren S.P., Reddy B.D., Kuntz K., Lopez-Maury L., Thompson J., Watt S., Ma C., Hou H., Shi Y., Yates J.R. III, Bahler J., O'Connell M.J., Jia S.
    J. Biol. Chem. 287:4386-4393(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MST2 COMPLEX, FUNCTION.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMST2_SCHPO
AccessioniPrimary (citable) accession number: Q10325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3