Reviewed, UniProtKB/Swiss-Prot Q10295 (PAP_SCHPO)

Names and origin

Protein names

Recommended name:
    Poly(A) polymerase pla1
      Short name=PAP
    EC=2.7.7.19
Alternative name(s):
    Polynucleotide adenylyltransferase

Gene names

Name:	pla1
ORF Names:	SPBC646.04

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CF I). Ref.1
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Nucleus. Ref.3
Sequence similarities	Belongs to the poly(A) polymerase family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA polyadenylation Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe transcription Inferred from electronic annotation. Source: InterPro
Cellular component	mRNA cleavage and polyadenylation specificity factor complex Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Poly(A) polymerase pla1		PRO_0000051620
Sites
	Active site	99	1	By similarity
	Active site	101	1	By similarity
	Active site	153	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10295-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E8E0E4165AAFD3D5	FASTA	566	64,109
	10 20 30 40 50 60 MTTKQWGITP PISTAPATEQ ENALNTALIN ELKNQNLFES PAESEKRVKV LDELQQITTE 70 80 90 100 110 120 FVKKVSLAKH MNEKMANEAG GKIFTYGSYR LGVYGPGSDI DTLVVVPKHV SRDNFFQDLE 130 140 150 160 170 180 PMLREREEVT DLAAVPDAYV PIIKFKFLGI SIDLIFARLS VPRVPRDLEL SDNNLLKGVE 190 200 210 220 230 240 ERCVLSLNGT RVTDQILQLV PNRAVFKHAL RAIKFWAQRR AIYANVVGFP GGVAWAMMVA 250 260 270 280 290 300 RICQLYPNAV SSVIVAKFFR ILHQWNWPQP ILLKPIEDGP LQVRIWNPKL YPSDKAHRMP 310 320 330 340 350 360 IITPAYPSMC ATHNITLSTQ TIILREMVRA GEIADQIMVK ALPWSALFQK HDFFHRYKHY 370 380 390 400 410 420 LTITAAAKTA EAQLKWAGLV ESKLRHLVTR LELVDAIALA HPFNKGFDKV YNCSSEEEAQ 430 440 450 460 470 480 QVASGVTLEV AYESTDHEKL ANDTVNEEKA DNTESKADGS ENGEKQIFPV YTTTCYIGLE 490 500 510 520 530 540 LEKKKGHPIK RLDISWPTQE FYELCKKWDK YDDTLMNVFI KNTKNTALPD EVFEPGEERP 550 560 KATKKRSTAD TAHSTEQLKR QKVSTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue." Ohnacker M., Minivielle-Sebastia L., Keller W. Nucleic Acids Res. 24:2585-2591(1996) [PubMed: 8692700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 233-436, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79705 mRNA. Translation: CAA56141.1. CU329671 Genomic DNA. Translation: CAA22808.1. AB027883 Genomic DNA. Translation: BAA87187.1.
PIR	JC6058.
RefSeq	NP_595362.1.
3D structure databases
HSSP	HSSP built from PDB template 1FA0 based on UniProtKB P29468.
ModBase	Search...
Genome annotation databases
GeneID	2541099.
KEGG	spo:SPBC646.04.
NMPDR	fig|4896.1.peg.1228.
Organism-specific databases
GeneDB_Spombe	SPBC646.04.
Phylogenomic databases
OMA	Q10295. WSWPQPV.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003438-MON.
BRENDA	2.7.7.19. 653.
Gene expression databases
ArrayExpress	Q10295.
Family and domain databases
InterPro	IPR002934. Nucleotidyltransferase. IPR007012. PolA_pol_centr. IPR007010. PolA_pol_RNA-bd. IPR014492. PolyA_polymerase. [Graphical view]
Gene3D	G3DSA:3.30.70.590. PolA_pol_RNA-bd. 1 hit.
Pfam	PF01909. NTP_transf_2. 1 hit. PF04928. PAP_central. 1 hit. PF04926. PAP_RNA-bind. 1 hit. [Graphical view]
PIRSF	PIRSF018425. PolyA_polymerase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP_SCHPO

Accession

Primary (citable) accession number: Q10295
Secondary accession number(s): Q9UU09

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)