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Q10295 (PAP_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase pla1

Short name=PAP
EC=2.7.7.19
Alternative name(s):
Polynucleotide adenylyltransferase
Gene names
Name:pla1
ORF Names:SPBC646.04
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CF I). Ref.1

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subcellular location

Nucleus Ref.3.

Sequence similarities

Belongs to the poly(A) polymerase family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566Poly(A) polymerase pla1
PRO_0000051620

Regions

Nucleotide binding86 – 883ATP By similarity
Nucleotide binding99 – 1013ATP By similarity
Nucleotide binding232 – 2332ATP By similarity

Sites

Metal binding991Magnesium 1; catalytic By similarity
Metal binding991Magnesium 2; catalytic By similarity
Metal binding1011Magnesium 1; catalytic By similarity
Metal binding1011Magnesium 2; catalytic By similarity
Metal binding1531Magnesium 2; catalytic By similarity
Binding site1531ATP By similarity
Binding site2141ATP By similarity
Binding site2231ATP By similarity
Site1441Interaction with RNA By similarity
Site3131Interaction with RNA By similarity
Site3141Interaction with RNA By similarity
Site3851Interaction with RNA By similarity
Site5001Interaction with RNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q10295 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E8E0E4165AAFD3D5

FASTA56664,109
        10         20         30         40         50         60 
MTTKQWGITP PISTAPATEQ ENALNTALIN ELKNQNLFES PAESEKRVKV LDELQQITTE 

        70         80         90        100        110        120 
FVKKVSLAKH MNEKMANEAG GKIFTYGSYR LGVYGPGSDI DTLVVVPKHV SRDNFFQDLE 

       130        140        150        160        170        180 
PMLREREEVT DLAAVPDAYV PIIKFKFLGI SIDLIFARLS VPRVPRDLEL SDNNLLKGVE 

       190        200        210        220        230        240 
ERCVLSLNGT RVTDQILQLV PNRAVFKHAL RAIKFWAQRR AIYANVVGFP GGVAWAMMVA 

       250        260        270        280        290        300 
RICQLYPNAV SSVIVAKFFR ILHQWNWPQP ILLKPIEDGP LQVRIWNPKL YPSDKAHRMP 

       310        320        330        340        350        360 
IITPAYPSMC ATHNITLSTQ TIILREMVRA GEIADQIMVK ALPWSALFQK HDFFHRYKHY 

       370        380        390        400        410        420 
LTITAAAKTA EAQLKWAGLV ESKLRHLVTR LELVDAIALA HPFNKGFDKV YNCSSEEEAQ 

       430        440        450        460        470        480 
QVASGVTLEV AYESTDHEKL ANDTVNEEKA DNTESKADGS ENGEKQIFPV YTTTCYIGLE 

       490        500        510        520        530        540 
LEKKKGHPIK RLDISWPTQE FYELCKKWDK YDDTLMNVFI KNTKNTALPD EVFEPGEERP 

       550        560 
KATKKRSTAD TAHSTEQLKR QKVSTA 

« Hide

References

« Hide 'large scale' references
[1]"The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue."
Ohnacker M., Minivielle-Sebastia L., Keller W.
Nucleic Acids Res. 24:2585-2591(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 233-436, SUBCELLULAR LOCATION.
Strain: ATCC 38364 / 968.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79705 mRNA. Translation: CAA56141.1.
CU329671 Genomic DNA. Translation: CAA22808.1.
AB027883 Genomic DNA. Translation: BAA87187.1.
PIRJC6058.
RefSeqNP_595362.1. NM_001021270.2.

3D structure databases

ProteinModelPortalQ10295.
SMRQ10295. Positions 2-534.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277614. 5 interactions.
IntActQ10295. 5 interactions.
MINTMINT-2730388.
STRING4896.SPBC646.04-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC646.04.1; SPBC646.04.1:pep; SPBC646.04.
GeneID2541099.
KEGGspo:SPBC646.04.

Organism-specific databases

PomBaseSPBC646.04.

Phylogenomic databases

eggNOGCOG5186.
HOGENOMHOG000204376.
KOK14376.
OMAMGIVVRH.
OrthoDBEOG7M6DHG.
PhylomeDBQ10295.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Other

NextBio20802212.
PROQ10295.

Entry information

Entry namePAP_SCHPO
AccessionPrimary (citable) accession number: Q10295
Secondary accession number(s): Q9UU09
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names