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Q10295

- PAP_SCHPO

UniProt

Q10295 - PAP_SCHPO

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Protein

Poly(A) polymerase pla1

Gene

pla1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CF I).1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Magnesium 1; catalyticBy similarity
Metal bindingi99 – 991Magnesium 2; catalyticBy similarity
Metal bindingi101 – 1011Magnesium 1; catalyticBy similarity
Metal bindingi101 – 1011Magnesium 2; catalyticBy similarity
Sitei144 – 1441Interaction with RNABy similarity
Metal bindingi153 – 1531Magnesium 2; catalyticBy similarity
Binding sitei153 – 1531ATPBy similarity
Binding sitei214 – 2141ATPBy similarity
Binding sitei223 – 2231ATPBy similarity
Sitei313 – 3131Interaction with RNABy similarity
Sitei314 – 3141Interaction with RNABy similarity
Sitei385 – 3851Interaction with RNABy similarity
Sitei500 – 5001Interaction with RNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 883ATPBy similarity
Nucleotide bindingi99 – 1013ATPBy similarity
Nucleotide bindingi232 – 2332ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: PomBase
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase pla1 (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
Polynucleotide adenylyltransferase
Gene namesi
Name:pla1
ORF Names:SPBC646.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC646.04.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. mRNA cleavage and polyadenylation specificity factor complex Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Poly(A) polymerase pla1PRO_0000051620Add
BLAST

Proteomic databases

MaxQBiQ10295.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mmi1O749583EBI-7997221,EBI-7997069
red1Q9UTR83EBI-7997221,EBI-1117407
SPAC17H9.02O137992EBI-7997221,EBI-8993901

Protein-protein interaction databases

BioGridi277614. 5 interactions.
IntActiQ10295. 5 interactions.
MINTiMINT-2730388.
STRINGi4896.SPBC646.04-1.

Structurei

3D structure databases

ProteinModelPortaliQ10295.
SMRiQ10295. Positions 2-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiCOG5186.
HOGENOMiHOG000204376.
InParanoidiQ10295.
KOiK14376.
OMAiMGIVVRH.
OrthoDBiEOG7M6DHG.
PhylomeDBiQ10295.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10295-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTKQWGITP PISTAPATEQ ENALNTALIN ELKNQNLFES PAESEKRVKV
60 70 80 90 100
LDELQQITTE FVKKVSLAKH MNEKMANEAG GKIFTYGSYR LGVYGPGSDI
110 120 130 140 150
DTLVVVPKHV SRDNFFQDLE PMLREREEVT DLAAVPDAYV PIIKFKFLGI
160 170 180 190 200
SIDLIFARLS VPRVPRDLEL SDNNLLKGVE ERCVLSLNGT RVTDQILQLV
210 220 230 240 250
PNRAVFKHAL RAIKFWAQRR AIYANVVGFP GGVAWAMMVA RICQLYPNAV
260 270 280 290 300
SSVIVAKFFR ILHQWNWPQP ILLKPIEDGP LQVRIWNPKL YPSDKAHRMP
310 320 330 340 350
IITPAYPSMC ATHNITLSTQ TIILREMVRA GEIADQIMVK ALPWSALFQK
360 370 380 390 400
HDFFHRYKHY LTITAAAKTA EAQLKWAGLV ESKLRHLVTR LELVDAIALA
410 420 430 440 450
HPFNKGFDKV YNCSSEEEAQ QVASGVTLEV AYESTDHEKL ANDTVNEEKA
460 470 480 490 500
DNTESKADGS ENGEKQIFPV YTTTCYIGLE LEKKKGHPIK RLDISWPTQE
510 520 530 540 550
FYELCKKWDK YDDTLMNVFI KNTKNTALPD EVFEPGEERP KATKKRSTAD
560
TAHSTEQLKR QKVSTA
Length:566
Mass (Da):64,109
Last modified:November 1, 1996 - v1
Checksum:iE8E0E4165AAFD3D5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79705 mRNA. Translation: CAA56141.1.
CU329671 Genomic DNA. Translation: CAA22808.1.
AB027883 Genomic DNA. Translation: BAA87187.1.
PIRiJC6058.
RefSeqiNP_595362.1. NM_001021270.2.

Genome annotation databases

EnsemblFungiiSPBC646.04.1; SPBC646.04.1:pep; SPBC646.04.
GeneIDi2541099.
KEGGispo:SPBC646.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79705 mRNA. Translation: CAA56141.1 .
CU329671 Genomic DNA. Translation: CAA22808.1 .
AB027883 Genomic DNA. Translation: BAA87187.1 .
PIRi JC6058.
RefSeqi NP_595362.1. NM_001021270.2.

3D structure databases

ProteinModelPortali Q10295.
SMRi Q10295. Positions 2-534.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277614. 5 interactions.
IntActi Q10295. 5 interactions.
MINTi MINT-2730388.
STRINGi 4896.SPBC646.04-1.

Proteomic databases

MaxQBi Q10295.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC646.04.1 ; SPBC646.04.1:pep ; SPBC646.04 .
GeneIDi 2541099.
KEGGi spo:SPBC646.04.

Organism-specific databases

PomBasei SPBC646.04.

Phylogenomic databases

eggNOGi COG5186.
HOGENOMi HOG000204376.
InParanoidi Q10295.
KOi K14376.
OMAi MGIVVRH.
OrthoDBi EOG7M6DHG.
PhylomeDBi Q10295.

Miscellaneous databases

NextBioi 20802212.
PROi Q10295.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue."
    Ohnacker M., Minivielle-Sebastia L., Keller W.
    Nucleic Acids Res. 24:2585-2591(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 233-436, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.

Entry informationi

Entry nameiPAP_SCHPO
AccessioniPrimary (citable) accession number: Q10295
Secondary accession number(s): Q9UU09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3