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Protein

3-hydroxy-3-methylglutaryl-coenzyme A reductase

Gene

hmg1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of HMG-CoA to mevalonate. It is the rate-limiting enzyme of the sterol biosynthesis pathway. Involved in ergosterol biosynthesis.2 Publications

Catalytic activityi

(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Acetyl-CoA acetyltransferase (erg10)
  2. Hydroxymethylglutaryl-CoA synthase (hcs1)
  3. 3-hydroxy-3-methylglutaryl-coenzyme A reductase (hmg1)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei712 – 7121Charge relay systemBy similarity
Active sitei846 – 8461Charge relay systemBy similarity
Active sitei922 – 9221Charge relay systemBy similarity
Active sitei1018 – 10181Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

  • coenzyme A metabolic process Source: InterPro
  • ergosterol biosynthetic process Source: PomBase
  • farnesyl diphosphate biosynthetic process, mevalonate pathway Source: PomBase
  • isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-SPO-191273. Cholesterol biosynthesis.
UniPathwayiUPA00058; UER00103.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC:1.1.1.34)
Short name:
HMG-CoA reductase
Gene namesi
Name:hmg1
Synonyms:its12
ORF Names:SPCC162.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC162.09c.
PomBaseiSPCC162.09c. hmg1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicBy similarity
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 203174LumenalBy similarityAdd
BLAST
Transmembranei204 – 22421HelicalSequence analysisAdd
BLAST
Topological domaini225 – 2328CytoplasmicBy similarity
Transmembranei233 – 25321HelicalSequence analysisAdd
BLAST
Topological domaini254 – 2585LumenalBy similarity
Transmembranei259 – 27921HelicalSequence analysisAdd
BLAST
Topological domaini280 – 32041CytoplasmicBy similarityAdd
BLAST
Transmembranei321 – 34121HelicalSequence analysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 41755CytoplasmicSequence analysisAdd
BLAST
Transmembranei418 – 43821HelicalSequence analysisAdd
BLAST
Topological domaini439 – 52688LumenalSequence analysisAdd
BLAST
Transmembranei527 – 54721HelicalSequence analysisAdd
BLAST
Topological domaini548 – 1053506CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: PomBase
  • endoplasmic reticulum membrane Source: PomBase
  • integral component of membrane Source: UniProtKB-KW
  • nuclear membrane Source: PomBase
  • nuclear outer membrane-endoplasmic reticulum membrane network Source: PomBase
  • peroxisomal membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1163121.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 105310533-hydroxy-3-methylglutaryl-coenzyme A reductasePRO_0000114455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Glycosylationi518 – 5181N-linked (GlcNAc...)Sequence analysis
Modified residuei1024 – 10241Phosphoserine1 Publication
Modified residuei1028 – 10281Phosphothreonine1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ10283.

PTM databases

iPTMnetiQ10283.

Interactioni

Protein-protein interaction databases

BioGridi275573. 1 interaction.

Chemistry

BindingDBiQ10283.

Structurei

3D structure databases

ProteinModelPortaliQ10283.
SMRiQ10283. Positions 595-1015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 365162SSDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni548 – 61568LinkerAdd
BLAST
Regioni616 – 1053438CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the HMG-CoA reductase family.Curated
Contains 1 SSD (sterol-sensing) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000183489.
InParanoidiQ10283.
KOiK00021.
OrthoDBiEOG092C3BIQ.
PhylomeDBiQ10283.

Family and domain databases

CDDicd00643. HMG-CoA_reductase_classI. 1 hit.
Gene3Di1.10.3270.10. 1 hit.
3.30.70.420. 1 hit.
3.90.770.10. 2 hits.
InterProiIPR002202. HMG_CoA_Rdtase.
IPR023074. HMG_CoA_Rdtase_cat.
IPR023076. HMG_CoA_Rdtase_CS.
IPR004554. HMG_CoA_Rdtase_eu_arc.
IPR023282. HMG_CoA_Rdtase_N.
IPR009023. HMG_CoA_Rdtase_NAD(P)-bd_dom.
IPR009029. HMG_CoA_Rdtase_sub-bd_dom.
IPR000731. SSD.
[Graphical view]
PANTHERiPTHR10572. PTHR10572. 2 hits.
PfamiPF00368. HMG-CoA_red. 1 hit.
PF12349. Sterol-sensing. 1 hit.
[Graphical view]
PRINTSiPR00071. HMGCOARDTASE.
SUPFAMiSSF55035. SSF55035. 1 hit.
SSF56542. SSF56542. 2 hits.
TIGRFAMsiTIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITEiPS00066. HMG_COA_REDUCTASE_1. 1 hit.
PS00318. HMG_COA_REDUCTASE_2. 1 hit.
PS50065. HMG_COA_REDUCTASE_4. 1 hit.
PS50156. SSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYKLAARYP IQVIAIVGIL VSMAYFSFLE ALTQEDFPVL IRALKRFGIL
60 70 80 90 100
DGFPNTRLPN EMILKLSSVQ GEDASVWEQI PAAELGGEGF VDFDITQWYY
110 120 130 140 150
PANAKVDVAQ LVEPYRNDCI FHDASGACHF FFKEVGNWTV SSIALPSNLA
160 170 180 190 200
NPPIDYFLDS SSTVIQRILP AIREHGISWS WLLQLIARTW MNTLKIASQA
210 220 230 240 250
SKTELLIVGT AYACMLISIV SLYLKMRRLG SKFWLFFSVL LSTLFSVQFA
260 270 280 290 300
MTLVRASGVR ISLVSLIESL PFLINVVALD KAAELTRQVI TRCSVSDSHS
310 320 330 340 350
PMHEDIAKAC RNAAPPILRH FSFGIVVLAI FSYCNFGIKQ FFLFAAVMIY
360 370 380 390 400
DLLLLFSFFV AILTLKLEMR RYNAKDDVRK VLIEEGLSES TARHVADGND
410 420 430 440 450
SSATTSAGSR YFKVRYGTKI ILFIFIAFNL FELCSIPFKH YAATSAAAAR
460 470 480 490 500
LIPLVRSQYP DFKSQRLLDD GVFDDVLSAI SSMSNIESPS VRLLPAVFYG
510 520 530 540 550
AELSSTSFLS TIHSFINNWS HYISASFLSK WIVCALSLSI AVNVFLLNAA
560 570 580 590 600
RLNSIKEEPE KKVVEKVVEV VKYIPSSNSS SIDDIQKDEI AQESVVRSLE
610 620 630 640 650
ECITLYNNGQ ISTLNDEEVV QLTLAKKIPL YALERVLKDV TRAVVIRRTV
660 670 680 690 700
VSRSSRTKTL ESSNCPVYHY DYSRVLNACC ENVIGYMPLP LGVAGPLIID
710 720 730 740 750
GKPFYIPMAT TEGALVASTM RGCKAINAGG GAVTVLTRDQ MSRGPCVAFP
760 770 780 790 800
NLTRAGRAKI WLDSPEGQEV MKKAFNSTSR FARLQHIKTA LAGTRLFIRF
810 820 830 840 850
CTSTGDAMGM NMISKGVEHA LVVMSNDAGF DDMQVISVSG NYCTDKKPAA
860 870 880 890 900
INWIDGRGKS VIAEAIIPGD AVKSVLKTTV EDLVKLNVDK NLIGSAMAGS
910 920 930 940 950
VGGFNAHAAN IVTAVYLATG QDPAQNVESS NCITLMDNVD GNLQLSVSMP
960 970 980 990 1000
SIEVGTIGGG TVLEPQGAML DLLGVRGAHM TSPGDNSRQL ARVVAAAVMA
1010 1020 1030 1040 1050
GELSLCSALA SGHLVKSHIG LNRSALNTPA MDSSAKKPAT DALKSVNSRV

PGR
Length:1,053
Mass (Da):114,877
Last modified:December 1, 2000 - v2
Checksum:i33E5C2365222D238
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti751 – 7511N → D in AAB39277 (PubMed:8896278).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76979 Genomic DNA. Translation: AAB39277.1.
CU329672 Genomic DNA. Translation: CAA19589.1.
PIRiS72194.
RefSeqiNP_588235.1. NM_001023225.2.

Genome annotation databases

EnsemblFungiiSPCC162.09c.1; SPCC162.09c.1:pep; SPCC162.09c.
GeneIDi2538999.
KEGGispo:SPCC162.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76979 Genomic DNA. Translation: AAB39277.1.
CU329672 Genomic DNA. Translation: CAA19589.1.
PIRiS72194.
RefSeqiNP_588235.1. NM_001023225.2.

3D structure databases

ProteinModelPortaliQ10283.
SMRiQ10283. Positions 595-1015.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275573. 1 interaction.

Chemistry

BindingDBiQ10283.
ChEMBLiCHEMBL1163121.

PTM databases

iPTMnetiQ10283.

Proteomic databases

MaxQBiQ10283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC162.09c.1; SPCC162.09c.1:pep; SPCC162.09c.
GeneIDi2538999.
KEGGispo:SPCC162.09c.

Organism-specific databases

EuPathDBiFungiDB:SPCC162.09c.
PomBaseiSPCC162.09c. hmg1.

Phylogenomic databases

HOGENOMiHOG000183489.
InParanoidiQ10283.
KOiK00021.
OrthoDBiEOG092C3BIQ.
PhylomeDBiQ10283.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00103.
ReactomeiR-SPO-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiQ10283.

Family and domain databases

CDDicd00643. HMG-CoA_reductase_classI. 1 hit.
Gene3Di1.10.3270.10. 1 hit.
3.30.70.420. 1 hit.
3.90.770.10. 2 hits.
InterProiIPR002202. HMG_CoA_Rdtase.
IPR023074. HMG_CoA_Rdtase_cat.
IPR023076. HMG_CoA_Rdtase_CS.
IPR004554. HMG_CoA_Rdtase_eu_arc.
IPR023282. HMG_CoA_Rdtase_N.
IPR009023. HMG_CoA_Rdtase_NAD(P)-bd_dom.
IPR009029. HMG_CoA_Rdtase_sub-bd_dom.
IPR000731. SSD.
[Graphical view]
PANTHERiPTHR10572. PTHR10572. 2 hits.
PfamiPF00368. HMG-CoA_red. 1 hit.
PF12349. Sterol-sensing. 1 hit.
[Graphical view]
PRINTSiPR00071. HMGCOARDTASE.
SUPFAMiSSF55035. SSF55035. 1 hit.
SSF56542. SSF56542. 2 hits.
TIGRFAMsiTIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITEiPS00066. HMG_COA_REDUCTASE_1. 1 hit.
PS00318. HMG_COA_REDUCTASE_2. 1 hit.
PS50065. HMG_COA_REDUCTASE_4. 1 hit.
PS50156. SSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMDH_SCHPO
AccessioniPrimary (citable) accession number: Q10283
Secondary accession number(s): O74425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.