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Protein

Guanine nucleotide-binding protein subunit beta-like protein

Gene

rkp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a receptor for protein kinase C in the regulation of actin cytoskeleton organization during cell wall synthesis and morphogenesis.1 Publication

GO - Molecular functioni

  • protein kinase C binding Source: PomBase
  • ribosome binding Source: PomBase

GO - Biological processi

  • conjugation with cellular fusion Source: PomBase
  • negative regulation of cytoplasmic translation Source: PomBase
  • positive regulation of protein autophosphorylation Source: PomBase
  • regulation of eIF2 alpha phosphorylation by amino acid starvation Source: PomBase
  • regulation of fungal-type cell wall biogenesis Source: PomBase
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-like protein
Alternative name(s):
Receptor of activated protein kinase C
Gene namesi
Name:rkp1
Synonyms:cpc2
ORF Names:SPAC6B12.15
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6B12.15.
PomBaseiSPAC6B12.15.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Guanine nucleotide-binding protein subunit beta-like proteinPRO_0000127757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Phosphothreonine1 Publication
Modified residuei39 – 391Phosphoserine1 Publication
Modified residuei52 – 521Phosphotyrosine1 Publication
Modified residuei148 – 1481Phosphoserine1 Publication
Modified residuei242 – 2421Phosphoserine1 Publication
Modified residuei255 – 2551Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10281.

PTM databases

iPTMnetiQ10281.

Interactioni

Subunit structurei

Interacts with pck2.

Binary interactionsi

WithEntry#Exp.IntActNotes
ded1O133703EBI-696304,EBI-2478405
nrd1Q097024EBI-696304,EBI-696291
rpl3202P790152EBI-696304,EBI-7169357
sds23Q098263EBI-696304,EBI-7169035

GO - Molecular functioni

  • protein kinase C binding Source: PomBase

Protein-protein interaction databases

BioGridi279723. 13 interactions.
IntActiQ10281. 6 interactions.
MINTiMINT-1203489.

Structurei

3D structure databases

ProteinModelPortaliQ10281.
SMRiQ10281. Positions 4-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati13 – 4432WD 1Add
BLAST
Repeati61 – 9131WD 2Add
BLAST
Repeati103 – 13331WD 3Add
BLAST
Repeati146 – 17833WD 4Add
BLAST
Repeati190 – 22031WD 5Add
BLAST
Repeati231 – 26030WD 6Add
BLAST
Repeati281 – 31131WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000091643.
InParanoidiQ10281.
KOiK14753.
OMAiKHESEMA.
OrthoDBiEOG73Z348.
PhylomeDBiQ10281.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10281-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEQLVLRAT LEGHSGWVTS LSTAPENPDI LLSGSRDKSI ILWNLVRDDV
60 70 80 90 100
NYGVAQRRLT GHSHFVSDCA LSFDSHYALS ASWDKTIRLW DLEKGECTHQ
110 120 130 140 150
FVGHTSDVLS VSISPDNRQV VSGSRDKTIK IWNIIGNCKY TITDGGHSDW
160 170 180 190 200
VSCVRFSPNP DNLTFVSAGW DKAVKVWDLE TFSLRTSHYG HTGYVSAVTI
210 220 230 240 250
SPDGSLCASG GRDGTLMLWD LNESTHLYSL EAKANINALV FSPNRYWLCA
260 270 280 290 300
ATGSSIRIFD LETQEKVDEL TVDFVGVGKK SSEPECISLT WSPDGQTLFS
310
GWTDNLIRVW QVTK
Length:314
Mass (Da):34,851
Last modified:August 29, 2001 - v3
Checksum:i4E14707164E68ACD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411I → L in AAA56865 (Ref. 1) Curated
Sequence conflicti41 – 411I → L in AAK38633 (PubMed:11263963).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37885 mRNA. Translation: AAA56865.2.
AF320333 Genomic DNA. Translation: AAK38633.1.
CU329670 Genomic DNA. Translation: CAB11079.1.
D89247 mRNA. Translation: BAA13908.1.
PIRiT43158.
T43299.
RefSeqiNP_593770.1. NM_001019200.2.

Genome annotation databases

EnsemblFungiiSPAC6B12.15.1; SPAC6B12.15.1:pep; SPAC6B12.15.
GeneIDi2543298.
KEGGispo:SPAC6B12.15.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37885 mRNA. Translation: AAA56865.2.
AF320333 Genomic DNA. Translation: AAK38633.1.
CU329670 Genomic DNA. Translation: CAB11079.1.
D89247 mRNA. Translation: BAA13908.1.
PIRiT43158.
T43299.
RefSeqiNP_593770.1. NM_001019200.2.

3D structure databases

ProteinModelPortaliQ10281.
SMRiQ10281. Positions 4-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279723. 13 interactions.
IntActiQ10281. 6 interactions.
MINTiMINT-1203489.

PTM databases

iPTMnetiQ10281.

Proteomic databases

MaxQBiQ10281.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6B12.15.1; SPAC6B12.15.1:pep; SPAC6B12.15.
GeneIDi2543298.
KEGGispo:SPAC6B12.15.

Organism-specific databases

EuPathDBiFungiDB:SPAC6B12.15.
PomBaseiSPAC6B12.15.

Phylogenomic databases

HOGENOMiHOG000091643.
InParanoidiQ10281.
KOiK14753.
OMAiKHESEMA.
OrthoDBiEOG73Z348.
PhylomeDBiQ10281.

Miscellaneous databases

NextBioi20804316.
PROiQ10281.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and nucleotide sequence of Schizosaccharomyces pombe homologue of the receptor for activated protein kinase C gene."
    Park S.-K., Yoo H.-S.
    J. Microbiol. 33:128-131(1995)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ED616.
  2. "Rkp1/Cpc2, a fission yeast RACK1 homolog, is involved in actin cytoskeleton organization through protein kinase C, Pck2, signaling."
    Won M., Park S.-K., Hoe K.-L., Jang Y.-J., Chung K.-S., Kim D.-U., Kim H.-B., Yoo H.-S.
    Biochem. Biophys. Res. Commun. 282:10-15(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: ED616.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-314.
    Strain: PR745.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-10; SER-39; TYR-52; SER-148; SER-242 AND SER-255, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGBLP_SCHPO
AccessioniPrimary (citable) accession number: Q10281
Secondary accession number(s): P78896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 29, 2001
Last modified: May 11, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.