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Protein

Probable phosphoadenosine phosphosulfate reductase

Gene

met16

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier (By similarity). Required for methionine synthesis.By similarity1 Publication

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (sua1)
  2. Adenylyl-sulfate kinase (met14)
  3. Probable phosphoadenosine phosphosulfate reductase (met16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAdoPS reductase
Gene namesi
Name:met16
ORF Names:SPAC13G7.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13G7.06.
PomBaseiSPAC13G7.06. met16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to methionine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Probable phosphoadenosine phosphosulfate reductasePRO_0000100661Add
BLAST

Proteomic databases

MaxQBiQ10270.

Interactioni

Protein-protein interaction databases

BioGridi279282. 17 interactions.
MINTiMINT-1214622.

Structurei

3D structure databases

ProteinModelPortaliQ10270.
SMRiQ10270. Positions 11-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000249397.
InParanoidiQ10270.
KOiK00390.
OMAiPRNKLLD.
OrthoDBiEOG092C4JOA.
PhylomeDBiQ10270.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIDTPANK LQAKTLFHPE HLEYINKQLS ELSPQDILKW CRWTLPSLFQ
60 70 80 90 100
TSALGLSGLV IMDMLSKMDM NVPLIFINTL HHFPETLDLL EKVKTKYPNV
110 120 130 140 150
PVHVYRCAEA ANEKEFAQKF GEKLWETDES RYDFLVKVEP ASRAYSDLNV
160 170 180 190 200
LAVFTGRRRS QGGERGSLPI VQLDGPVLKI NPLANWSFTE VHNYIITNNV
210 220 230 240 250
PYNELLNKGY RSVGDWHSTQ PVREGEDERA GRWRGREKTE CGLHSHPQSK
260
FAQYMAELKK KETADQ
Length:266
Mass (Da):30,561
Last modified:October 1, 1996 - v1
Checksum:i6243A03A03317170
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93594.1.
PIRiS67435.
RefSeqiNP_593708.1. NM_001019139.2.

Genome annotation databases

EnsemblFungiiSPAC13G7.06.1; SPAC13G7.06.1:pep; SPAC13G7.06.
GeneIDi2542836.
KEGGispo:SPAC13G7.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93594.1.
PIRiS67435.
RefSeqiNP_593708.1. NM_001019139.2.

3D structure databases

ProteinModelPortaliQ10270.
SMRiQ10270. Positions 11-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279282. 17 interactions.
MINTiMINT-1214622.

Proteomic databases

MaxQBiQ10270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC13G7.06.1; SPAC13G7.06.1:pep; SPAC13G7.06.
GeneIDi2542836.
KEGGispo:SPAC13G7.06.

Organism-specific databases

EuPathDBiFungiDB:SPAC13G7.06.
PomBaseiSPAC13G7.06. met16.

Phylogenomic databases

HOGENOMiHOG000249397.
InParanoidiQ10270.
KOiK00390.
OMAiPRNKLLD.
OrthoDBiEOG092C4JOA.
PhylomeDBiQ10270.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206.

Miscellaneous databases

PROiQ10270.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMET16_SCHPO
AccessioniPrimary (citable) accession number: Q10270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.