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Protein

Probable phosphoadenosine phosphosulfate reductase

Gene

met16

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier (By similarity). Required for methionine synthesis.By similarity1 Publication

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (sua1)
  2. Adenylyl-sulfate kinase (met14)
  3. Probable phosphoadenosine phosphosulfate reductase (met16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAdoPS reductase
Gene namesi
Name:met16
ORF Names:SPAC13G7.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13G7.06
PomBaseiSPAC13G7.06 met16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to methionine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001006611 – 266Probable phosphoadenosine phosphosulfate reductaseAdd BLAST266

Proteomic databases

MaxQBiQ10270
PaxDbiQ10270
PRIDEiQ10270

Interactioni

Protein-protein interaction databases

BioGridi279282, 17 interactors
MINTiQ10270
STRINGi4896.SPAC13G7.06.1

Structurei

3D structure databases

ProteinModelPortaliQ10270
SMRiQ10270
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000249397
InParanoidiQ10270
KOiK00390
OMAiVGDWHSS
OrthoDBiEOG092C4JOA
PhylomeDBiQ10270

Family and domain databases

CDDicd01713 PAPS_reductase, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR004511 PAPS/APS_Rdtase
IPR002500 PAPS_reduct
IPR011800 PAPS_reductase_CysH
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01507 PAPS_reduct, 1 hit
PIRSFiPIRSF000857 PAPS_reductase, 1 hit
TIGRFAMsiTIGR00434 cysH, 1 hit
TIGR02057 PAPS_reductase, 1 hit

Sequencei

Sequence statusi: Complete.

Q10270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIDTPANK LQAKTLFHPE HLEYINKQLS ELSPQDILKW CRWTLPSLFQ
60 70 80 90 100
TSALGLSGLV IMDMLSKMDM NVPLIFINTL HHFPETLDLL EKVKTKYPNV
110 120 130 140 150
PVHVYRCAEA ANEKEFAQKF GEKLWETDES RYDFLVKVEP ASRAYSDLNV
160 170 180 190 200
LAVFTGRRRS QGGERGSLPI VQLDGPVLKI NPLANWSFTE VHNYIITNNV
210 220 230 240 250
PYNELLNKGY RSVGDWHSTQ PVREGEDERA GRWRGREKTE CGLHSHPQSK
260
FAQYMAELKK KETADQ
Length:266
Mass (Da):30,561
Last modified:October 1, 1996 - v1
Checksum:i6243A03A03317170
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA93594.1
PIRiS67435
RefSeqiNP_593708.1, NM_001019139.2

Genome annotation databases

EnsemblFungiiSPAC13G7.06.1; SPAC13G7.06.1:pep; SPAC13G7.06
GeneIDi2542836
KEGGispo:SPAC13G7.06

Similar proteinsi

Entry informationi

Entry nameiMET16_SCHPO
AccessioniPrimary (citable) accession number: Q10270
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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