Q10270 (MET16_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAdoPS reductase

Gene names

ORF Names:

SPAC13G7.06

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier By similarity.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Methionine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Probable phosphoadenosine phosphosulfate reductase		PRO_0000100661

Sequences

	Sequence		Length	Mass (Da)
	Q10270 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6243A03A03317170	FASTA	266	30,561
	10 20 30 40 50 60 MSSIDTPANK LQAKTLFHPE HLEYINKQLS ELSPQDILKW CRWTLPSLFQ TSALGLSGLV 70 80 90 100 110 120 IMDMLSKMDM NVPLIFINTL HHFPETLDLL EKVKTKYPNV PVHVYRCAEA ANEKEFAQKF 130 140 150 160 170 180 GEKLWETDES RYDFLVKVEP ASRAYSDLNV LAVFTGRRRS QGGERGSLPI VQLDGPVLKI 190 200 210 220 230 240 NPLANWSFTE VHNYIITNNV PYNELLNKGY RSVGDWHSTQ PVREGEDERA GRWRGREKTE 250 260 CGLHSHPQSK FAQYMAELKK KETADQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA93594.1.
PIR	S67435.
RefSeq	NP_593708.1. NM_001019139.1.
3D structure databases
ProteinModelPortal	Q10270.
SMR	Q10270. Positions 11-254.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10270. 1 interaction.
MINT	MINT-1214622.
STRING	Q10270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC13G7.06.1; SPAC13G7.06.1:pep; SPAC13G7.06.
GeneID	2542836.
KEGG	spo:SPAC13G7.06.
NMPDR	fig|4896.1.peg.3678.
Organism-specific databases
GeneDB_Spombe	SPAC13G7.06.
Phylogenomic databases
eggNOG	fuNOG04624.
GeneTree	EFGT00050000005384.
HOGENOM	HBG758022.
OMA	FEETLAY.
OrthoDB	EOG4W3WX4.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001372-MONOMER.
Gene expression databases
ArrayExpress	Q10270.
Family and domain databases
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MET16_SCHPO

Accession

Primary (citable) accession number: Q10270

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program