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Q10258

- MTHR1_SCHPO

UniProt

Q10258 - MTHR1_SCHPO

Protein

Methylenetetrahydrofolate reductase 1

Gene

met9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Major methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine. Performs 80 to 85 percent of the total methylenetetrahydrofolate reductase activity of the cells.1 Publication

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.1 Publication

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei21 – 211Proton donor/acceptorBy similarity
    Binding sitei82 – 821FADBy similarity
    Binding sitei114 – 1141SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei173 – 1731FADBy similarity
    Binding sitei184 – 1841SubstrateBy similarity
    Binding sitei276 – 2761SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 266NADBy similarity
    Nucleotide bindingi53 – 542NAD and FADBy similarity
    Nucleotide bindingi112 – 1143FADBy similarity
    Nucleotide bindingi130 – 1312FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: PomBase

    GO - Biological processi

    1. methionine biosynthetic process Source: PomBase
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
    Gene namesi
    Name:met9
    ORF Names:SPAC56F8.10
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC56F8.10.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. mitochondrion Source: PomBase
    3. nucleus Source: PomBase

    Pathology & Biotechi

    Disruption phenotypei

    Leads to strict auxotrophy for methionine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 603603Methylenetetrahydrofolate reductase 1PRO_0000190253Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei355 – 3551Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ10258.

    Interactioni

    Protein-protein interaction databases

    BioGridi279431. 1 interaction.
    MINTiMINT-4698197.
    STRINGi4896.SPAC56F8.10-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    KOiK00297.
    OMAiYLEFFVS.
    OrthoDBiEOG7M0P15.
    PhylomeDBiQ10258.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q10258-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKISDKLLHP DWKEKVTYSY EFFPPKTSTG VQNLYNRIDR MKTWGRPMFV    50
    DVTWGAGGTS SELTPGIVNV IQTDFEVDTC MHLTCTNMST EMIDAALKRA 100
    HETGCRNILA LRGDPVKDTD WTEGESGFRY ASDLVRYIRT HYNDEFCIGV 150
    AGYPEGYSPD DDIDESIKHL KLKVDEGADF IVTQMFYDVD NFIAWVDKVR 200
    AAGINIPIFP GIMPIQAWDS FIRRAKWSGV KIPQHFMDTL VPVKDDDEGV 250
    RERGVELIVE MCRKLIASGI TRLHFYTMNL EKAVKMIIER LGLLDENLAP 300
    IVDTNNVELT NASSQDRRIN EGVRPIFWRT RNESYVSRTD QWDELPHGRW 350
    GDSRSPAFGE FDAIRYGLRM SPKEITTSWG SPKSYSEIGD LFARYCEKKI 400
    SSLPWSDLPI SDEADLIRDQ LLSMNRNAFL TINSQPALNG EKSSHPVFGW 450
    GPPNGYVFQK PYVEFFVHPS LLNELKETVK KLNSVSYFVT NKNGDLDTNS 500
    QYEIPNAVTW GVFPNREIIQ PTIVESTSFL AWKDEAYSLG MEWANAYSPD 550
    SISRKLLVSM MKEWFLCVIV DNDFQNGQSL FDVFNKMRSL KDIHPELYYA 600
    NAS 603
    Length:603
    Mass (Da):69,012
    Last modified:October 1, 1996 - v1
    Checksum:i38519FEE783D331F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93581.1.
    PIRiT38920.
    RefSeqiNP_593224.1. NM_001018621.2.

    Genome annotation databases

    EnsemblFungiiSPAC56F8.10.1; SPAC56F8.10.1:pep; SPAC56F8.10.
    GeneIDi2542993.
    KEGGispo:SPAC56F8.10.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93581.1 .
    PIRi T38920.
    RefSeqi NP_593224.1. NM_001018621.2.

    3D structure databases

    ProteinModelPortali Q10258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279431. 1 interaction.
    MINTi MINT-4698197.
    STRINGi 4896.SPAC56F8.10-1.

    Proteomic databases

    MaxQBi Q10258.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC56F8.10.1 ; SPAC56F8.10.1:pep ; SPAC56F8.10 .
    GeneIDi 2542993.
    KEGGi spo:SPAC56F8.10.

    Organism-specific databases

    PomBasei SPAC56F8.10.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    KOi K00297.
    OMAi YLEFFVS.
    OrthoDBi EOG7M0P15.
    PhylomeDBi Q10258.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Miscellaneous databases

    NextBioi 20804026.
    PROi Q10258.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe."
      Naula N., Walther C., Baumann D., Schweingruber M.E.
      Yeast 19:841-848(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiMTHR1_SCHPO
    AccessioniPrimary (citable) accession number: Q10258
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3