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Protein

25S rRNA (adenine(645)-N(1))-methyltransferase

Gene

rrp8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2 (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + adenine(645) in 25S rRNA = S-adenosyl-L-homocysteine + N1-methyladenine(645) in 25S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132S-adenosyl-L-methionineBy similarity1
Binding sitei175S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei197S-adenosyl-L-methionineBy similarity1
Binding sitei209S-adenosyl-L-methionineBy similarity1
Binding sitei226S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-427359 SIRT1 negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (adenine(645)-N(1))-methyltransferase (EC:2.1.1.287)
Alternative name(s):
Ribosomal RNA-processing protein 8
Gene namesi
Name:rrp8
ORF Names:SPAC56F8.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC56F8.09
PomBaseiSPAC56F8.09 rrp8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001165691 – 31825S rRNA (adenine(645)-N(1))-methyltransferaseAdd BLAST318

Proteomic databases

MaxQBiQ10257
PaxDbiQ10257
PRIDEiQ10257

Interactioni

Protein-protein interaction databases

BioGridi279647, 10 interactors
STRINGi4896.SPAC56F8.09.1

Structurei

3D structure databases

ProteinModelPortaliQ10257
SMRiQ10257
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 96Lys-richAdd BLAST77

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000164004
InParanoidiQ10257
KOiK14850
OMAiCLREAWR
OrthoDBiEOG092C2Q7B
PhylomeDBiQ10257

Family and domain databases

InterProiView protein in InterPro
IPR007823 RRP8
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12787 PTHR12787, 1 hit
PfamiView protein in Pfam
PF05148 Methyltransf_8, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q10257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKVDWDLGP VSKSASDQTK ESRKEKKRKK GERKNVGDKG EKLNEKVLKK
60 70 80 90 100
AKSVTTNNSL KSEIKKEKSV PSIKEKNKGD AKHTKLTSLQ QKMKDKLDGA
110 120 130 140 150
NFRWINEQLY TTESDKAVQM FKENPDLFDI YHAGFRYQVE GWPENPVDIF
160 170 180 190 200
IQHLKIRFEH SNAKKKNNIV IADLGCGEAK IASTFRKSRS LQVHSFDLVA
210 220 230 240 250
PNEHVVACDI ANVPMADETV DIAVFCLSLM GTNWQSFLKE AYRILKVGGL
260 270 280 290 300
LWVAEIKSRF SDKSGEVFAK ELPKLGFETK SIQLQNKMFT LFEFKKVPVH
310
GKCEELPPIL SACIYKRR
Length:318
Mass (Da):36,329
Last modified:October 1, 1996 - v1
Checksum:i623181910971F721
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA93580.1
PIRiT38919
RefSeqiNP_593223.1, NM_001018620.2

Genome annotation databases

EnsemblFungiiSPAC56F8.09.1; SPAC56F8.09.1:pep; SPAC56F8.09
GeneIDi2543219
KEGGispo:SPAC56F8.09

Similar proteinsi

Entry informationi

Entry nameiRRP8_SCHPO
AccessioniPrimary (citable) accession number: Q10257
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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