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Protein

25S rRNA (adenine(645)-N(1))-methyltransferase

Gene

rrp8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2 (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + adenine(645) in 25S rRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(645) in 25S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei132 – 1321S-adenosyl-L-methionineBy similarity
Binding sitei175 – 1751S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei197 – 1971S-adenosyl-L-methionineBy similarity
Binding sitei209 – 2091S-adenosyl-L-methionineBy similarity
Binding sitei226 – 2261S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (adenine(645)-N(1))-methyltransferase (EC:2.1.1.287)
Alternative name(s):
Ribosomal RNA-processing protein 8
Gene namesi
Name:rrp8
ORF Names:SPAC56F8.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC56F8.09.
PomBaseiSPAC56F8.09. rrp8.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31831825S rRNA (adenine(645)-N(1))-methyltransferasePRO_0000116569Add
BLAST

Proteomic databases

MaxQBiQ10257.

Interactioni

Protein-protein interaction databases

BioGridi279647. 10 interactions.
MINTiMINT-4698188.

Structurei

3D structure databases

ProteinModelPortaliQ10257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi20 – 9677Lys-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000164004.
InParanoidiQ10257.
KOiK14850.
OMAiKWPVNPV.
OrthoDBiEOG7WX0KP.
PhylomeDBiQ10257.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. RRP8.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 1 hit.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKVDWDLGP VSKSASDQTK ESRKEKKRKK GERKNVGDKG EKLNEKVLKK
60 70 80 90 100
AKSVTTNNSL KSEIKKEKSV PSIKEKNKGD AKHTKLTSLQ QKMKDKLDGA
110 120 130 140 150
NFRWINEQLY TTESDKAVQM FKENPDLFDI YHAGFRYQVE GWPENPVDIF
160 170 180 190 200
IQHLKIRFEH SNAKKKNNIV IADLGCGEAK IASTFRKSRS LQVHSFDLVA
210 220 230 240 250
PNEHVVACDI ANVPMADETV DIAVFCLSLM GTNWQSFLKE AYRILKVGGL
260 270 280 290 300
LWVAEIKSRF SDKSGEVFAK ELPKLGFETK SIQLQNKMFT LFEFKKVPVH
310
GKCEELPPIL SACIYKRR
Length:318
Mass (Da):36,329
Last modified:October 1, 1996 - v1
Checksum:i623181910971F721
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93580.1.
PIRiT38919.
RefSeqiNP_593223.1. NM_001018620.2.

Genome annotation databases

EnsemblFungiiSPAC56F8.09.1; SPAC56F8.09.1:pep; SPAC56F8.09.
GeneIDi2543219.
KEGGispo:SPAC56F8.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93580.1.
PIRiT38919.
RefSeqiNP_593223.1. NM_001018620.2.

3D structure databases

ProteinModelPortaliQ10257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279647. 10 interactions.
MINTiMINT-4698188.

Proteomic databases

MaxQBiQ10257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC56F8.09.1; SPAC56F8.09.1:pep; SPAC56F8.09.
GeneIDi2543219.
KEGGispo:SPAC56F8.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC56F8.09.
PomBaseiSPAC56F8.09. rrp8.

Phylogenomic databases

HOGENOMiHOG000164004.
InParanoidiQ10257.
KOiK14850.
OMAiKWPVNPV.
OrthoDBiEOG7WX0KP.
PhylomeDBiQ10257.

Miscellaneous databases

PROiQ10257.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. RRP8.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 1 hit.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRRP8_SCHPO
AccessioniPrimary (citable) accession number: Q10257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.