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Q10254 (ALG10_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

EC=2.4.1.256
Alternative name(s):
Alpha-1,2-glucosyltransferase alg10
Alpha-2-glucosyltransferase alg10
Asparagine-linked glycosylation protein 10
Dolichyl-phosphoglucose-dependent glucosyltransferase alg10
Gene names
Name:alg10
ORF Names:SPAC56F8.06c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.

Catalytic activity

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ALG10 glucosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
PRO_0000215460

Regions

Transmembrane7 – 2721Helical; Potential
Transmembrane54 – 7421Helical; Potential
Transmembrane81 – 10121Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane170 – 19021Helical; Potential
Transmembrane192 – 21221Helical; Potential
Transmembrane218 – 23821Helical; Potential
Transmembrane256 – 27621Helical; Potential
Transmembrane298 – 31821Helical; Potential
Transmembrane336 – 35621Helical; Potential
Transmembrane364 – 38421Helical; Potential
Transmembrane408 – 42821Helical; Potential

Amino acid modifications

Glycosylation761N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q10254 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D031A9914058AE69

FASTA44552,279
        10         20         30         40         50         60 
MFLGKAIILI LWIFLAFTGL LAIQYYVPNP YLDEIFHIAQ AQRFCRKDWD WDPAITTPPG 

        70         80         90        100        110        120 
LYLVSVALSP FIGCSNVSLR LINWLVGVIG LPWLINDIVS LLNNRKGDVV TYFAYTLSSL 

       130        140        150        160        170        180 
PPLWFFSFLY YTDIGSTFFV LLAYDFALRK SAFSSSVSCF FSLWFRQTNI VWMVFIAVTY 

       190        200        210        220        230        240 
FASNMSFFNP HLAEATFADV LLTIISFLGV FLKNLRRFSC PILSYGAVFC SFLAFLLWNG 

       250        260        270        280        290        300 
SIVLGDKSHH QASIHLSQIN YFLWFFFFFS FPSYIIKYLM SHSRRSKLLS AVFSKKSFLI 

       310        320        330        340        350        360 
VSVLLLIAHF NTIFHPFILA DNRHYLFYVF NRLFRIWWLK YLGPFSYLIL YYFFLDISKL 

       370        380        390        400        410        420 
QMTSLTFFLL ISTTILTLVP APLVEFRYFL LPFLFWRFHL PLPSGRECLM EYALHMVINS 

       430        440 
VTLYIFLFRT FIWPSEPNAL QRFMW 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329670 Genomic DNA. Translation: CAA93577.1.
PIRT38916.
RefSeqNP_593221.1. NM_001018617.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ10254.

Protein family/group databases

CAZyGT59. Glycosyltransferase Family 59.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC56F8.06c.1; SPAC56F8.06c.1:pep; SPAC56F8.06c.
GeneID2543245.
GenomeReviewsGene locus alg10 in contig CU329670_GR.
KEGGspo:SPAC56F8.06c.
NMPDRfig|4896.1.peg.3191.

Organism-specific databases

GeneDB_SpombeSPAC56F8.06c.

Phylogenomic databases

eggNOGfuNOG05358.
GeneTreeEFGT00050000003395.
HOGENOMHBG714502.
OMALLTWPYI.
OrthoDBEOG47WRXP.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-000914-MONOMER.

Gene expression databases

ArrayExpressQ10254.

Family and domain databases

InterProIPR016900. Alpha1_2_glucosyltferase_Alg10.
IPR007006. Glycosyltransferase_ALG10.
[Graphical view]
KOK03850.
PANTHERPTHR12989. DIE2_ALG10. 1 hit.
PfamPF04922. DIE2_ALG10. 1 hit.
[Graphical view]
PIRSFPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALG10_SCHPO
AccessionPrimary (citable) accession number: Q10254
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families