Reviewed, UniProtKB/Swiss-Prot Q10254 (ALG10_SCHPO)

Names and origin

Protein names

Recommended name:
    Alpha-1,2 glucosyltransferase alg10
    EC=2.4.1.-
Alternative name(s):
    Alpha-2-glucosyltransferase alg10
    Dolichyl-phosphoglucose-dependent glucosyltransferase alg10
    Asparagine-linked glycosylation protein 10

Gene names

Name:	alg10
ORF Names:	SPAC56F8.06c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ALG10 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Alpha-1,2 glucosyltransferase alg10		PRO_0000215460
Regions
	Transmembrane	7 – 27	21	Potential
	Transmembrane	54 – 74	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	123 – 143	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	192 – 212	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	256 – 276	21	Potential
	Transmembrane	298 – 318	21	Potential
	Transmembrane	336 – 356	21	Potential
	Transmembrane	364 – 384	21	Potential
	Transmembrane	408 – 428	21	Potential
Amino acid modifications
	Glycosylation	76	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10254-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D031A9914058AE69	FASTA	445	52,279
	10 20 30 40 50 60 MFLGKAIILI LWIFLAFTGL LAIQYYVPNP YLDEIFHIAQ AQRFCRKDWD WDPAITTPPG 70 80 90 100 110 120 LYLVSVALSP FIGCSNVSLR LINWLVGVIG LPWLINDIVS LLNNRKGDVV TYFAYTLSSL 130 140 150 160 170 180 PPLWFFSFLY YTDIGSTFFV LLAYDFALRK SAFSSSVSCF FSLWFRQTNI VWMVFIAVTY 190 200 210 220 230 240 FASNMSFFNP HLAEATFADV LLTIISFLGV FLKNLRRFSC PILSYGAVFC SFLAFLLWNG 250 260 270 280 290 300 SIVLGDKSHH QASIHLSQIN YFLWFFFFFS FPSYIIKYLM SHSRRSKLLS AVFSKKSFLI 310 320 330 340 350 360 VSVLLLIAHF NTIFHPFILA DNRHYLFYVF NRLFRIWWLK YLGPFSYLIL YYFFLDISKL 370 380 390 400 410 420 QMTSLTFFLL ISTTILTLVP APLVEFRYFL LPFLFWRFHL PLPSGRECLM EYALHMVINS 430 440 VTLYIFLFRT FIWPSEPNAL QRFMW

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA93577.1.
PIR	T38916.
RefSeq	NP_593221.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT59. Glycosyltransferase Family 59.
Genome annotation databases
GeneID	2543245.
KEGG	spo:SPAC56F8.06c.
NMPDR	fig|4896.1.peg.3191.
Organism-specific databases
GeneDB_Spombe	SPAC56F8.06c.
Phylogenomic databases
OMA	Q10254. WKFTYVH.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000914-MON.
Gene expression databases
ArrayExpress	Q10254.
Family and domain databases
InterPro	IPR016900. Alpha1_2_glucosyltferase_Alg10. IPR007006. Glycosyltransferase_ALG10. [Graphical view]
PANTHER	PTHR12989. DIE2_ALG10. 1 hit.
Pfam	PF04922. DIE2_ALG10. 1 hit. [Graphical view]
PIRSF	PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALG10_SCHPO

Accession

Primary (citable) accession number: Q10254

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)