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Protein

Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

alg10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc2-PP-Dol.

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378

Protein family/group databases

CAZyiGT59 Glycosyltransferase Family 59

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC:2.4.1.256)
Alternative name(s):
Alpha-1,2-glucosyltransferase alg10
Alpha-2-glucosyltransferase alg10
Asparagine-linked glycosylation protein 10
Dolichyl-phosphoglucose-dependent glucosyltransferase alg10
Gene namesi
Name:alg10
ORF Names:SPAC56F8.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC56F8.06c
PomBaseiSPAC56F8.06c alg10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Transmembranei364 – 384HelicalSequence analysisAdd BLAST21
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002154601 – 445Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi76N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi239N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ10254
PRIDEiQ10254

Interactioni

Protein-protein interaction databases

BioGridi279673, 23 interactors
STRINGi4896.SPAC56F8.06c.1

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG10 glucosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000240902
InParanoidiQ10254
KOiK03850
OMAiWWFRYAM
OrthoDBiEOG092C321K
PhylomeDBiQ10254

Family and domain databases

InterProiView protein in InterPro
IPR016900 Alg10
PANTHERiPTHR12989 PTHR12989, 1 hit
PfamiView protein in Pfam
PF04922 DIE2_ALG10, 1 hit
PIRSFiPIRSF028810 Alpha1_2_glucosyltferase_Alg10, 1 hit

Sequencei

Sequence statusi: Complete.

Q10254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLGKAIILI LWIFLAFTGL LAIQYYVPNP YLDEIFHIAQ AQRFCRKDWD
60 70 80 90 100
WDPAITTPPG LYLVSVALSP FIGCSNVSLR LINWLVGVIG LPWLINDIVS
110 120 130 140 150
LLNNRKGDVV TYFAYTLSSL PPLWFFSFLY YTDIGSTFFV LLAYDFALRK
160 170 180 190 200
SAFSSSVSCF FSLWFRQTNI VWMVFIAVTY FASNMSFFNP HLAEATFADV
210 220 230 240 250
LLTIISFLGV FLKNLRRFSC PILSYGAVFC SFLAFLLWNG SIVLGDKSHH
260 270 280 290 300
QASIHLSQIN YFLWFFFFFS FPSYIIKYLM SHSRRSKLLS AVFSKKSFLI
310 320 330 340 350
VSVLLLIAHF NTIFHPFILA DNRHYLFYVF NRLFRIWWLK YLGPFSYLIL
360 370 380 390 400
YYFFLDISKL QMTSLTFFLL ISTTILTLVP APLVEFRYFL LPFLFWRFHL
410 420 430 440
PLPSGRECLM EYALHMVINS VTLYIFLFRT FIWPSEPNAL QRFMW
Length:445
Mass (Da):52,279
Last modified:October 1, 1996 - v1
Checksum:iD031A9914058AE69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA93577.1
PIRiT38916
RefSeqiNP_593221.1, NM_001018617.2

Genome annotation databases

EnsemblFungiiSPAC56F8.06c.1; SPAC56F8.06c.1:pep; SPAC56F8.06c
GeneIDi2543245
KEGGispo:SPAC56F8.06c

Similar proteinsi

Entry informationi

Entry nameiALG10_SCHPO
AccessioniPrimary (citable) accession number: Q10254
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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