Q10251 (IF2P_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 5B
Short name=eIF-5B

Alternative name(s):
Translation initiation factor IF-2

Gene names

ORF Names:

SPAC56F8.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	1079 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translational initiation Non-traceable author statement. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	GTP binding Inferred by curator. Source: PomBase GTPase activity Inferred from sequence orthology. Source: PomBase translation initiation factor activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1079	1079	Eukaryotic translation initiation factor 5B		PRO_0000137295
Regions
	Nucleotide binding	491 – 498	8	GTP By similarity
Amino acid modifications
	Modified residue	73	1	Phosphoserine Ref.2
	Modified residue	77	1	Phosphoserine Ref.2
	Modified residue	82	1	Phosphoserine Ref.2
	Modified residue	127	1	Phosphoserine Ref.2
	Modified residue	364	1	Phosphothreonine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q10251 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 73A01CE933C1F6AA	FASTA	1,079	119,931
	10 20 30 40 50 60 MGKKGKKSGY ADWEDDLGED ISGQNEYLDN TSQDSPQNDE LAEKSENLAV SSEKTTSKKK 70 80 90 100 110 120 KGKKNKGNKN QVSDDESQEL ESPQGPKELT AVTELDDDEF DYKPKKGKKG KKSKKVEEDD 130 140 150 160 170 180 EPQEIESPQG PKELTAVTEL DDDEFDYKPK KGKKGKKAQN NNESEAAAPP EIPEVRVKTK 190 200 210 220 230 240 KEKEREKKER EKLRKKQQQA KKKGSTGEDT LASSEVSSEV DISTPAENDS SAKGKQAAGS 250 260 270 280 290 300 KRKGPNVTAL QKMLEEKRAR EEEEQRIREE EARIAEEEKR LAEVEEARKE EARLKKKEKE 310 320 330 340 350 360 RKKKEEMKAQ GKYLSKKQKE QQALAQRRLQ QMLESGVRVA GLSNGEKKQK PVYTNKKKSN 370 380 390 400 410 420 RSGTSSISSS GILESSPATS ISVDEPQKDS KDDSEKVEKE TEVERKEENE AEAEAVFDDW 430 440 450 460 470 480 EAALEEPEVA ENNEVVTEKK ETDIKSDAVE HSIKDKEDSK TDKVDDIPQA APAESNVSES 490 500 510 520 530 540 DLRSPICCIL GHVDTGKTKL LDNLRRSNVQ EGEAGGITQQ IGATYFPIES IKQKTKVVNK 550 560 570 580 590 600 KGKLQYNIPG LLIIDTPGHE SFTNLRSRGT SLCNIAILVI DIMHGLEPQT IESIRLLRDQ 610 620 630 640 650 660 KTPFVVALNK VDRLYGWHSI KDNAIQDSLS KQKKAIQREF RDRVESIILQ LNEQGLNAAL 670 680 690 700 710 720 YFENKNLGRY VSLVPTSAQS GEGVPDLVAL LISLTQTRMS DRIKYITTLE CTVLEVKVIE 730 740 750 760 770 780 GLGATIDVIL SNGVLHEGDR IVLCGMGGPI ITTVRALLTP QPLKEMRVKS AYVHHKEIKA 790 800 810 820 830 840 AMGVKICAND LEKAVAGSRL LVVGPDDDEE DLAEEIMEDL ENLLGRIDTS GIGVSVQAST 850 860 870 880 890 900 LGSLEALLEF LKQMKIPVAS VNIGPVYKKD VMRCATMLEK AKEYALMLCF DVKVDRDAED 910 920 930 940 950 960 LAEQLGVKIF SANVIYHLFD AFTAHQKKIL EQKREESSDV AVFPCVLKTV AAFNKRDPII 970 980 990 1000 1010 1020 LGVDVVEGVL RINTPIVAVK QLPNGEPQII ELGRVASLEM NHKPVDKVKK GQAGAGVAMK 1030 1040 1050 1060 1070 LESSGSQILF GRQVTESDAL YSHITRQSID SLKDPAFRDE VSRDEWQLII QLKKLFGII

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-77; SER-82; SER-127 AND THR-364, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA93574.1.
PIR	T38913.
RefSeq	NP_593218.1. NM_001018614.2.
3D structure databases
ProteinModelPortal	Q10251.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC56F8.03-1.
Proteomic databases
PaxDb	Q10251.
PRIDE	Q10251.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC56F8.03.1; SPAC56F8.03.1:pep; SPAC56F8.03.
GeneID	2543241.
KEGG	spo:SPAC56F8.03.
Organism-specific databases
PomBase	SPAC56F8.03.
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000105770.
KO	K03243.
OMA	VMGVIVE.
OrthoDB	EOG40311D.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804263.

Entry information

Entry name

IF2P_SCHPO

Accession

Primary (citable) accession number: Q10251

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program