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Q10251

- IF2P_SCHPO

UniProt

Q10251 - IF2P_SCHPO

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Protein

Eukaryotic translation initiation factor 5B

Gene

SPAC56F8.03

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi491 – 4988GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: PomBase
  2. GTP binding Source: UniProtKB-KW
  3. translation initiation factor activity Source: PomBase

GO - Biological processi

  1. cytoplasmic translational initiation Source: PomBase
  2. GTP catabolic process Source: GOC
  3. translational initiation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_215930. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
ORF Names:SPAC56F8.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC56F8.03.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10791079Eukaryotic translation initiation factor 5BPRO_0000137295Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731Phosphoserine1 Publication
Modified residuei77 – 771Phosphoserine1 Publication
Modified residuei82 – 821Phosphoserine1 Publication
Modified residuei127 – 1271Phosphoserine1 Publication
Modified residuei364 – 3641Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10251.
PaxDbiQ10251.
PRIDEiQ10251.

Interactioni

Protein-protein interaction databases

BioGridi279669. 3 interactions.
MINTiMINT-4698132.
STRINGi4896.SPAC56F8.03-1.

Structurei

3D structure databases

ProteinModelPortaliQ10251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini482 – 700219tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni491 – 4988G1PROSITE-ProRule annotation
Regioni516 – 5205G2PROSITE-ProRule annotation
Regioni555 – 5584G3PROSITE-ProRule annotation
Regioni609 – 6124G4PROSITE-ProRule annotation
Regioni677 – 6793G5PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000105770.
InParanoidiQ10251.
KOiK03243.
OMAiEAKRKDA.
OrthoDBiEOG73BVNC.
PhylomeDBiQ10251.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10251 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKKGKKSGY ADWEDDLGED ISGQNEYLDN TSQDSPQNDE LAEKSENLAV
60 70 80 90 100
SSEKTTSKKK KGKKNKGNKN QVSDDESQEL ESPQGPKELT AVTELDDDEF
110 120 130 140 150
DYKPKKGKKG KKSKKVEEDD EPQEIESPQG PKELTAVTEL DDDEFDYKPK
160 170 180 190 200
KGKKGKKAQN NNESEAAAPP EIPEVRVKTK KEKEREKKER EKLRKKQQQA
210 220 230 240 250
KKKGSTGEDT LASSEVSSEV DISTPAENDS SAKGKQAAGS KRKGPNVTAL
260 270 280 290 300
QKMLEEKRAR EEEEQRIREE EARIAEEEKR LAEVEEARKE EARLKKKEKE
310 320 330 340 350
RKKKEEMKAQ GKYLSKKQKE QQALAQRRLQ QMLESGVRVA GLSNGEKKQK
360 370 380 390 400
PVYTNKKKSN RSGTSSISSS GILESSPATS ISVDEPQKDS KDDSEKVEKE
410 420 430 440 450
TEVERKEENE AEAEAVFDDW EAALEEPEVA ENNEVVTEKK ETDIKSDAVE
460 470 480 490 500
HSIKDKEDSK TDKVDDIPQA APAESNVSES DLRSPICCIL GHVDTGKTKL
510 520 530 540 550
LDNLRRSNVQ EGEAGGITQQ IGATYFPIES IKQKTKVVNK KGKLQYNIPG
560 570 580 590 600
LLIIDTPGHE SFTNLRSRGT SLCNIAILVI DIMHGLEPQT IESIRLLRDQ
610 620 630 640 650
KTPFVVALNK VDRLYGWHSI KDNAIQDSLS KQKKAIQREF RDRVESIILQ
660 670 680 690 700
LNEQGLNAAL YFENKNLGRY VSLVPTSAQS GEGVPDLVAL LISLTQTRMS
710 720 730 740 750
DRIKYITTLE CTVLEVKVIE GLGATIDVIL SNGVLHEGDR IVLCGMGGPI
760 770 780 790 800
ITTVRALLTP QPLKEMRVKS AYVHHKEIKA AMGVKICAND LEKAVAGSRL
810 820 830 840 850
LVVGPDDDEE DLAEEIMEDL ENLLGRIDTS GIGVSVQAST LGSLEALLEF
860 870 880 890 900
LKQMKIPVAS VNIGPVYKKD VMRCATMLEK AKEYALMLCF DVKVDRDAED
910 920 930 940 950
LAEQLGVKIF SANVIYHLFD AFTAHQKKIL EQKREESSDV AVFPCVLKTV
960 970 980 990 1000
AAFNKRDPII LGVDVVEGVL RINTPIVAVK QLPNGEPQII ELGRVASLEM
1010 1020 1030 1040 1050
NHKPVDKVKK GQAGAGVAMK LESSGSQILF GRQVTESDAL YSHITRQSID
1060 1070
SLKDPAFRDE VSRDEWQLII QLKKLFGII
Length:1,079
Mass (Da):119,931
Last modified:October 1, 1996 - v1
Checksum:i73A01CE933C1F6AA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA93574.1.
PIRiT38913.
RefSeqiNP_593218.1. NM_001018614.2.

Genome annotation databases

EnsemblFungiiSPAC56F8.03.1; SPAC56F8.03.1:pep; SPAC56F8.03.
GeneIDi2543241.
KEGGispo:SPAC56F8.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAA93574.1 .
PIRi T38913.
RefSeqi NP_593218.1. NM_001018614.2.

3D structure databases

ProteinModelPortali Q10251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279669. 3 interactions.
MINTi MINT-4698132.
STRINGi 4896.SPAC56F8.03-1.

Proteomic databases

MaxQBi Q10251.
PaxDbi Q10251.
PRIDEi Q10251.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC56F8.03.1 ; SPAC56F8.03.1:pep ; SPAC56F8.03 .
GeneIDi 2543241.
KEGGi spo:SPAC56F8.03.

Organism-specific databases

PomBasei SPAC56F8.03.

Phylogenomic databases

eggNOGi COG0532.
HOGENOMi HOG000105770.
InParanoidi Q10251.
KOi K03243.
OMAi EAKRKDA.
OrthoDBi EOG73BVNC.
PhylomeDBi Q10251.

Enzyme and pathway databases

Reactomei REACT_215930. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

NextBioi 20804263.
PROi Q10251.

Family and domain databases

Gene3Di 3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-77; SER-82; SER-127 AND THR-364, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiIF2P_SCHPO
AccessioniPrimary (citable) accession number: Q10251
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3