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Protein

Eukaryotic translation initiation factor 5B

Gene

SPAC56F8.03

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.By similarity

Catalytic activityi

GTP + H2O = GDP + phosphate.By similarity

Cofactori

a monovalent cationBy similarityNote: Binds 1 monovalent cation per monomer in the active site. Structural cofactor that stabilizes the GTP-bound "on" state. May also act as a transition state stabilizer of the hydrolysis reaction.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi491 – 4988GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B (EC:3.6.5.3)
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
ORF Names:SPAC56F8.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC56F8.03.
PomBaseiSPAC56F8.03.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytosol Source: PomBase
  • intracellular Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10791079Eukaryotic translation initiation factor 5BPRO_0000137295Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731Phosphoserine1 Publication
Modified residuei77 – 771Phosphoserine1 Publication
Modified residuei82 – 821Phosphoserine1 Publication
Modified residuei127 – 1271Phosphoserine1 Publication
Modified residuei364 – 3641Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10251.
PRIDEiQ10251.

PTM databases

iPTMnetiQ10251.

Interactioni

Protein-protein interaction databases

MINTiMINT-4698132.

Structurei

3D structure databases

ProteinModelPortaliQ10251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini482 – 700219tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni491 – 4988G1PROSITE-ProRule annotation
Regioni516 – 5205G2PROSITE-ProRule annotation
Regioni555 – 5584G3PROSITE-ProRule annotation
Regioni609 – 6124G4PROSITE-ProRule annotation
Regioni677 – 6793G5PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000105770.
InParanoidiQ10251.
KOiK03243.
OMAiRQQNEDV.
OrthoDBiEOG092C11L7.
PhylomeDBiQ10251.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKGKKSGY ADWEDDLGED ISGQNEYLDN TSQDSPQNDE LAEKSENLAV
60 70 80 90 100
SSEKTTSKKK KGKKNKGNKN QVSDDESQEL ESPQGPKELT AVTELDDDEF
110 120 130 140 150
DYKPKKGKKG KKSKKVEEDD EPQEIESPQG PKELTAVTEL DDDEFDYKPK
160 170 180 190 200
KGKKGKKAQN NNESEAAAPP EIPEVRVKTK KEKEREKKER EKLRKKQQQA
210 220 230 240 250
KKKGSTGEDT LASSEVSSEV DISTPAENDS SAKGKQAAGS KRKGPNVTAL
260 270 280 290 300
QKMLEEKRAR EEEEQRIREE EARIAEEEKR LAEVEEARKE EARLKKKEKE
310 320 330 340 350
RKKKEEMKAQ GKYLSKKQKE QQALAQRRLQ QMLESGVRVA GLSNGEKKQK
360 370 380 390 400
PVYTNKKKSN RSGTSSISSS GILESSPATS ISVDEPQKDS KDDSEKVEKE
410 420 430 440 450
TEVERKEENE AEAEAVFDDW EAALEEPEVA ENNEVVTEKK ETDIKSDAVE
460 470 480 490 500
HSIKDKEDSK TDKVDDIPQA APAESNVSES DLRSPICCIL GHVDTGKTKL
510 520 530 540 550
LDNLRRSNVQ EGEAGGITQQ IGATYFPIES IKQKTKVVNK KGKLQYNIPG
560 570 580 590 600
LLIIDTPGHE SFTNLRSRGT SLCNIAILVI DIMHGLEPQT IESIRLLRDQ
610 620 630 640 650
KTPFVVALNK VDRLYGWHSI KDNAIQDSLS KQKKAIQREF RDRVESIILQ
660 670 680 690 700
LNEQGLNAAL YFENKNLGRY VSLVPTSAQS GEGVPDLVAL LISLTQTRMS
710 720 730 740 750
DRIKYITTLE CTVLEVKVIE GLGATIDVIL SNGVLHEGDR IVLCGMGGPI
760 770 780 790 800
ITTVRALLTP QPLKEMRVKS AYVHHKEIKA AMGVKICAND LEKAVAGSRL
810 820 830 840 850
LVVGPDDDEE DLAEEIMEDL ENLLGRIDTS GIGVSVQAST LGSLEALLEF
860 870 880 890 900
LKQMKIPVAS VNIGPVYKKD VMRCATMLEK AKEYALMLCF DVKVDRDAED
910 920 930 940 950
LAEQLGVKIF SANVIYHLFD AFTAHQKKIL EQKREESSDV AVFPCVLKTV
960 970 980 990 1000
AAFNKRDPII LGVDVVEGVL RINTPIVAVK QLPNGEPQII ELGRVASLEM
1010 1020 1030 1040 1050
NHKPVDKVKK GQAGAGVAMK LESSGSQILF GRQVTESDAL YSHITRQSID
1060 1070
SLKDPAFRDE VSRDEWQLII QLKKLFGII
Length:1,079
Mass (Da):119,931
Last modified:October 1, 1996 - v1
Checksum:i73A01CE933C1F6AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93574.1.
PIRiT38913.
RefSeqiNP_593218.1. NM_001018614.2.

Genome annotation databases

EnsemblFungiiSPAC56F8.03.1; SPAC56F8.03.1:pep; SPAC56F8.03.
GeneIDi2543241.
KEGGispo:SPAC56F8.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93574.1.
PIRiT38913.
RefSeqiNP_593218.1. NM_001018614.2.

3D structure databases

ProteinModelPortaliQ10251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4698132.

PTM databases

iPTMnetiQ10251.

Proteomic databases

MaxQBiQ10251.
PRIDEiQ10251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC56F8.03.1; SPAC56F8.03.1:pep; SPAC56F8.03.
GeneIDi2543241.
KEGGispo:SPAC56F8.03.

Organism-specific databases

EuPathDBiFungiDB:SPAC56F8.03.
PomBaseiSPAC56F8.03.

Phylogenomic databases

HOGENOMiHOG000105770.
InParanoidiQ10251.
KOiK03243.
OMAiRQQNEDV.
OrthoDBiEOG092C11L7.
PhylomeDBiQ10251.

Enzyme and pathway databases

ReactomeiR-SPO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

PROiQ10251.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2P_SCHPO
AccessioniPrimary (citable) accession number: Q10251
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.