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Q10251

- IF2P_SCHPO

UniProt

Q10251 - IF2P_SCHPO

Protein

Eukaryotic translation initiation factor 5B

Gene

SPAC56F8.03

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi491 – 4988GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: PomBase
    2. GTP binding Source: UniProtKB-KW
    3. translation initiation factor activity Source: PomBase

    GO - Biological processi

    1. cytoplasmic translational initiation Source: PomBase
    2. GTP catabolic process Source: GOC
    3. translational initiation Source: PomBase

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_215930. GTP hydrolysis and joining of the 60S ribosomal subunit.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 5B
    Short name:
    eIF-5B
    Alternative name(s):
    Translation initiation factor IF-2
    Gene namesi
    ORF Names:SPAC56F8.03
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC56F8.03.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10791079Eukaryotic translation initiation factor 5BPRO_0000137295Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei73 – 731Phosphoserine1 Publication
    Modified residuei77 – 771Phosphoserine1 Publication
    Modified residuei82 – 821Phosphoserine1 Publication
    Modified residuei127 – 1271Phosphoserine1 Publication
    Modified residuei364 – 3641Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ10251.
    PaxDbiQ10251.
    PRIDEiQ10251.

    Interactioni

    Protein-protein interaction databases

    BioGridi279669. 3 interactions.
    MINTiMINT-4698132.
    STRINGi4896.SPAC56F8.03-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10251.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini482 – 700219tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni491 – 4988G1PROSITE-ProRule annotation
    Regioni516 – 5205G2PROSITE-ProRule annotation
    Regioni555 – 5584G3PROSITE-ProRule annotation
    Regioni609 – 6124G4PROSITE-ProRule annotation
    Regioni677 – 6793G5PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0532.
    HOGENOMiHOG000105770.
    KOiK03243.
    OMAiEAKRKDA.
    OrthoDBiEOG73BVNC.
    PhylomeDBiQ10251.

    Family and domain databases

    Gene3Di3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF11987. IF-2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q10251-1 [UniParc]FASTAAdd to Basket

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    MGKKGKKSGY ADWEDDLGED ISGQNEYLDN TSQDSPQNDE LAEKSENLAV     50
    SSEKTTSKKK KGKKNKGNKN QVSDDESQEL ESPQGPKELT AVTELDDDEF 100
    DYKPKKGKKG KKSKKVEEDD EPQEIESPQG PKELTAVTEL DDDEFDYKPK 150
    KGKKGKKAQN NNESEAAAPP EIPEVRVKTK KEKEREKKER EKLRKKQQQA 200
    KKKGSTGEDT LASSEVSSEV DISTPAENDS SAKGKQAAGS KRKGPNVTAL 250
    QKMLEEKRAR EEEEQRIREE EARIAEEEKR LAEVEEARKE EARLKKKEKE 300
    RKKKEEMKAQ GKYLSKKQKE QQALAQRRLQ QMLESGVRVA GLSNGEKKQK 350
    PVYTNKKKSN RSGTSSISSS GILESSPATS ISVDEPQKDS KDDSEKVEKE 400
    TEVERKEENE AEAEAVFDDW EAALEEPEVA ENNEVVTEKK ETDIKSDAVE 450
    HSIKDKEDSK TDKVDDIPQA APAESNVSES DLRSPICCIL GHVDTGKTKL 500
    LDNLRRSNVQ EGEAGGITQQ IGATYFPIES IKQKTKVVNK KGKLQYNIPG 550
    LLIIDTPGHE SFTNLRSRGT SLCNIAILVI DIMHGLEPQT IESIRLLRDQ 600
    KTPFVVALNK VDRLYGWHSI KDNAIQDSLS KQKKAIQREF RDRVESIILQ 650
    LNEQGLNAAL YFENKNLGRY VSLVPTSAQS GEGVPDLVAL LISLTQTRMS 700
    DRIKYITTLE CTVLEVKVIE GLGATIDVIL SNGVLHEGDR IVLCGMGGPI 750
    ITTVRALLTP QPLKEMRVKS AYVHHKEIKA AMGVKICAND LEKAVAGSRL 800
    LVVGPDDDEE DLAEEIMEDL ENLLGRIDTS GIGVSVQAST LGSLEALLEF 850
    LKQMKIPVAS VNIGPVYKKD VMRCATMLEK AKEYALMLCF DVKVDRDAED 900
    LAEQLGVKIF SANVIYHLFD AFTAHQKKIL EQKREESSDV AVFPCVLKTV 950
    AAFNKRDPII LGVDVVEGVL RINTPIVAVK QLPNGEPQII ELGRVASLEM 1000
    NHKPVDKVKK GQAGAGVAMK LESSGSQILF GRQVTESDAL YSHITRQSID 1050
    SLKDPAFRDE VSRDEWQLII QLKKLFGII 1079
    Length:1,079
    Mass (Da):119,931
    Last modified:October 1, 1996 - v1
    Checksum:i73A01CE933C1F6AA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93574.1.
    PIRiT38913.
    RefSeqiNP_593218.1. NM_001018614.2.

    Genome annotation databases

    EnsemblFungiiSPAC56F8.03.1; SPAC56F8.03.1:pep; SPAC56F8.03.
    GeneIDi2543241.
    KEGGispo:SPAC56F8.03.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAA93574.1 .
    PIRi T38913.
    RefSeqi NP_593218.1. NM_001018614.2.

    3D structure databases

    ProteinModelPortali Q10251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279669. 3 interactions.
    MINTi MINT-4698132.
    STRINGi 4896.SPAC56F8.03-1.

    Proteomic databases

    MaxQBi Q10251.
    PaxDbi Q10251.
    PRIDEi Q10251.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC56F8.03.1 ; SPAC56F8.03.1:pep ; SPAC56F8.03 .
    GeneIDi 2543241.
    KEGGi spo:SPAC56F8.03.

    Organism-specific databases

    PomBasei SPAC56F8.03.

    Phylogenomic databases

    eggNOGi COG0532.
    HOGENOMi HOG000105770.
    KOi K03243.
    OMAi EAKRKDA.
    OrthoDBi EOG73BVNC.
    PhylomeDBi Q10251.

    Enzyme and pathway databases

    Reactomei REACT_215930. GTP hydrolysis and joining of the 60S ribosomal subunit.

    Miscellaneous databases

    NextBioi 20804263.
    PROi Q10251.

    Family and domain databases

    Gene3Di 3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF11987. IF-2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-77; SER-82; SER-127 AND THR-364, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiIF2P_SCHPO
    AccessioniPrimary (citable) accession number: Q10251
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3