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Protein

Ribonuclease H2 subunit A

Gene

rnh201

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Divalent metal cationBy similarity
Metal bindingi70 – 701Divalent metal cationBy similarity
Metal bindingi180 – 1801Divalent metal cationBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: InterPro
  • RNA-DNA hybrid ribonuclease activity Source: PomBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit A (EC:3.1.26.4)
Short name:
RNase H2 subunit A
Alternative name(s):
Ribonuclease HI large subunit
Short name:
RNase HI large subunit
Ribonuclease HI subunit A
Gene namesi
Name:rnh201
ORF Names:SPAC4G9.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.02.
PomBaseiSPAC4G9.02. rnh201.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Ribonuclease H2 subunit APRO_0000111717Add
BLAST

Proteomic databases

MaxQBiQ10236.

Interactioni

Protein-protein interaction databases

BioGridi278042. 25 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ10236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. Eukaryotic subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000100290.
InParanoidiQ10236.
KOiK10743.
OMAiREECRFF.
OrthoDBiEOG76X68V.
PhylomeDBiQ10236.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q10236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDDHDAWEP EELVSDNNSS ENELQEDQNS SITFLPPSVN KSNPAKSNYY
60 70 80 90 100
HSTVTDDISK SQPYRLGVDE AGRGPVLGPM VYAVAYCPVD FDLTNYGFAD
110 120 130 140 150
SKTLASLKRE ELLKLICNKS NELGKNVGWS TMSISARELA AGMLRYRNKY
160 170 180 190 200
NLNLQAHDTT IDLIKKVYES GINVTEIYVD TVGPPISYQE KLQAHFPQAK
210 220 230 240 250
VTVTKKADSL FPIVSLASIC AKVTRDIQLE CARESIRTEN WGSGYSSDAR
260 270 280 290 300
TTEWLKVNVD KIFGWKGDIV RYSWKTAKDL LELPSKSQSS IEIDWHEDDD
310 320
TPTLNFTQKK KPNPASRSWF GSEFYF
Length:326
Mass (Da):36,808
Last modified:October 1, 1996 - v1
Checksum:i04E82E145CF0CCD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93552.1.
PIRiT38861.
RefSeqiNP_593684.1. NM_001019116.2.

Genome annotation databases

EnsemblFungiiSPAC4G9.02.1; SPAC4G9.02.1:pep; SPAC4G9.02.
GeneIDi2541542.
KEGGispo:SPAC4G9.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93552.1.
PIRiT38861.
RefSeqiNP_593684.1. NM_001019116.2.

3D structure databases

ProteinModelPortaliQ10236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278042. 25 interactions.

Proteomic databases

MaxQBiQ10236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4G9.02.1; SPAC4G9.02.1:pep; SPAC4G9.02.
GeneIDi2541542.
KEGGispo:SPAC4G9.02.

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.02.
PomBaseiSPAC4G9.02. rnh201.

Phylogenomic databases

HOGENOMiHOG000100290.
InParanoidiQ10236.
KOiK10743.
OMAiREECRFF.
OrthoDBiEOG76X68V.
PhylomeDBiQ10236.

Miscellaneous databases

PROiQ10236.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiRNH2A_SCHPO
AccessioniPrimary (citable) accession number: Q10236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.