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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

rpc2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1111 – 11111ZincBy similarity
Metal bindingi1114 – 11141ZincBy similarity
Metal bindingi1123 – 11231ZincBy similarity
Metal bindingi1126 – 11261ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1111 – 112616C4-typeBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit 2
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 130 kDa polypeptide
RPC130
Gene namesi
Name:rpc2
ORF Names:SPAC4G9.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.08c.
PomBaseiSPAC4G9.08c. rpc2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA-directed RNA polymerase III complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11651165DNA-directed RNA polymerase III subunit RPC2PRO_0000048095Add
BLAST

Proteomic databases

MaxQBiQ10233.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

BioGridi279815. 4 interactions.
DIPiDIP-29536N.
MINTiMINT-4697983.

Structurei

3D structure databases

ProteinModelPortaliQ10233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1111 – 112616C4-typeBy similarityAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000222962.
InParanoidiQ10233.
KOiK03021.
OMAiVCEDLIR.
OrthoDBiEOG7RRFGC.
PhylomeDBiQ10233.

Family and domain databases

Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVNTAGDPQ KSQPKINKGG IGKDESFGAL FKPVYKGKKL ADPVPTIEDK
60 70 80 90 100
WQLLPAFLKV KGLVKQHLDS YNYFVDVDLK KIVQANEKVT SDVEPWFYLK
110 120 130 140 150
YLDIRVGAPV RTDADAIQAS ISPHECRLRD LTYGANIYVD IEYTRGKQVV
160 170 180 190 200
RRRNVPIGRM PVMLRSNKCV LSGKNEMEMA ALNECPLDPG GYFIVKGTEK
210 220 230 240 250
VILVQEQLSK NRIIVEAEPK KGLWQASVTS STHERKSKTY VITKNGKLYL
260 270 280 290 300
KHNSVADDIP IVVVLKAMGL QSDQEIFELV AGAEASYQDL FAPSIEECAK
310 320 330 340 350
LNIYTAQQAL EYIGARVKVN RRAGANRLPP HEEALEVLAA VVLAHINVFN
360 370 380 390 400
LEFRPKAVYI GIMARRVLMA MVDPLQVDDR DYVGNKRLEL AGQLLALLFE
410 420 430 440 450
DLFKKFNSDL KLNIDKVLKK PHRTQEFDAY NQLTVHSDHI TQGMVRALST
460 470 480 490 500
GNWSLKRFKM ERAGVTHVLS RLSYISALGM MTRITSQFEK TRKVSGPRSL
510 520 530 540 550
QASQFGMLCT SDTPEGEACG LVKNLALMTH ITTDEEEEPI IKLAYAFGIE
560 570 580 590 600
DIHVISGREL HSHGTYLVYL NGAILGISRY PSLFVASFRK LRRSGKISPF
610 620 630 640 650
IGIFINTHQR AVFISTDGGR ICRPLIIVQN GLPKVESKHI RLLKEGKWGF
660 670 680 690 700
EDFLKQGLVE YVDVNEENDS LISVYERDIT PDTTHLEIEP FTILGAVAGL
710 720 730 740 750
IPYPHHNQSP RNTYQCAMGK QAIGAIAYNQ LQRIDTLLYL MVYPQQPMVK
760 770 780 790 800
TKTIELIGYD KLPAGQNATV AIMSYSGYDI EDALVLNKSS IDRGFGRCQV
810 820 830 840 850
FHKHSVIVRK YPNGTHDRIG DPQRDPETGE VVWKHGVVED DGLAGVGCRV
860 870 880 890 900
QPGQIYVNKQ TPTNALDNSI TLGHTQTVES GYKATPMTYK APEPGYIDKV
910 920 930 940 950
MLTTTDSDQT LIKVLMRQTR RPELGDKFSS RHGQKGVCGV IVQQEDMPFN
960 970 980 990 1000
DQGICPDIIM NPHGFPSRMT VGKMIELLSG KVGVLRGTLE YGTCFGGTKV
1010 1020 1030 1040 1050
EDASRILVEH GYNYSGKDML TSGITGETLE AYIFMGPIYY QKLKHMVMDK
1060 1070 1080 1090 1100
MHARARGPRA VLTRQPTEGR SRDGGLRLGE MERDCLIAYG ASQLLLERLM
1110 1120 1130 1140 1150
ISSDACDVDV CGQCGLLGYK GWCNSCQSTR EVVKMTIPYA AKLLFQELLS
1160
MNIVPRLALE DEFKY
Length:1,165
Mass (Da):130,238
Last modified:October 1, 1996 - v1
Checksum:i6690055F257EC0EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ156226 mRNA. Translation: ABA54854.1.
CU329670 Genomic DNA. Translation: CAA93558.1.
PIRiT38867.
RefSeqiNP_593690.1. NM_001019122.2.

Genome annotation databases

EnsemblFungiiSPAC4G9.08c.1; SPAC4G9.08c.1:pep; SPAC4G9.08c.
GeneIDi2543393.
KEGGispo:SPAC4G9.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ156226 mRNA. Translation: ABA54854.1.
CU329670 Genomic DNA. Translation: CAA93558.1.
PIRiT38867.
RefSeqiNP_593690.1. NM_001019122.2.

3D structure databases

ProteinModelPortaliQ10233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279815. 4 interactions.
DIPiDIP-29536N.
MINTiMINT-4697983.

Proteomic databases

MaxQBiQ10233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4G9.08c.1; SPAC4G9.08c.1:pep; SPAC4G9.08c.
GeneIDi2543393.
KEGGispo:SPAC4G9.08c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.08c.
PomBaseiSPAC4G9.08c. rpc2.

Phylogenomic databases

HOGENOMiHOG000222962.
InParanoidiQ10233.
KOiK03021.
OMAiVCEDLIR.
OrthoDBiEOG7RRFGC.
PhylomeDBiQ10233.

Enzyme and pathway databases

ReactomeiR-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.

Miscellaneous databases

PROiQ10233.

Family and domain databases

Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III."
    Proshkina G.M., Shematorova E.K., Proshkin S.A., Zaros C., Thuriaux P., Shpakovski G.V.
    Nucleic Acids Res. 34:3615-3624(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiRPC2_SCHPO
AccessioniPrimary (citable) accession number: Q10233
Secondary accession number(s): Q0QYE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.