Reviewed, UniProtKB/Swiss-Prot Q10233 (RPC2_SCHPO)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase III subunit RPC2
      Short name=RNA polymerase III subunit C2
      Short name=RNA polymerase III subunit 2
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase III 130 kDa polypeptide
    RPC130
    C128

Gene names

Name:	rpc2
ORF Names:	SPAC4G9.08c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription from RNA polymerase III promoter Non-traceable author statement. Source: GeneDB_SPombe
Cellular component	DNA-directed RNA polymerase III complex Non-traceable author statement. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Non-traceable author statement. Source: GeneDB_SPombe ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1165	1165	DNA-directed RNA polymerase III subunit RPC2		PRO_0000048095
Regions
	Zinc finger	1111 – 1126	16	C4-type By similarity
Sites
	Metal binding	1111	1	Zinc By similarity
	Metal binding	1114	1	Zinc By similarity
	Metal binding	1123	1	Zinc By similarity
	Metal binding	1126	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q10233-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6690055F257EC0EB	FASTA	1,165	130,238
	10 20 30 40 50 60 MGVNTAGDPQ KSQPKINKGG IGKDESFGAL FKPVYKGKKL ADPVPTIEDK WQLLPAFLKV 70 80 90 100 110 120 KGLVKQHLDS YNYFVDVDLK KIVQANEKVT SDVEPWFYLK YLDIRVGAPV RTDADAIQAS 130 140 150 160 170 180 ISPHECRLRD LTYGANIYVD IEYTRGKQVV RRRNVPIGRM PVMLRSNKCV LSGKNEMEMA 190 200 210 220 230 240 ALNECPLDPG GYFIVKGTEK VILVQEQLSK NRIIVEAEPK KGLWQASVTS STHERKSKTY 250 260 270 280 290 300 VITKNGKLYL KHNSVADDIP IVVVLKAMGL QSDQEIFELV AGAEASYQDL FAPSIEECAK 310 320 330 340 350 360 LNIYTAQQAL EYIGARVKVN RRAGANRLPP HEEALEVLAA VVLAHINVFN LEFRPKAVYI 370 380 390 400 410 420 GIMARRVLMA MVDPLQVDDR DYVGNKRLEL AGQLLALLFE DLFKKFNSDL KLNIDKVLKK 430 440 450 460 470 480 PHRTQEFDAY NQLTVHSDHI TQGMVRALST GNWSLKRFKM ERAGVTHVLS RLSYISALGM 490 500 510 520 530 540 MTRITSQFEK TRKVSGPRSL QASQFGMLCT SDTPEGEACG LVKNLALMTH ITTDEEEEPI 550 560 570 580 590 600 IKLAYAFGIE DIHVISGREL HSHGTYLVYL NGAILGISRY PSLFVASFRK LRRSGKISPF 610 620 630 640 650 660 IGIFINTHQR AVFISTDGGR ICRPLIIVQN GLPKVESKHI RLLKEGKWGF EDFLKQGLVE 670 680 690 700 710 720 YVDVNEENDS LISVYERDIT PDTTHLEIEP FTILGAVAGL IPYPHHNQSP RNTYQCAMGK 730 740 750 760 770 780 QAIGAIAYNQ LQRIDTLLYL MVYPQQPMVK TKTIELIGYD KLPAGQNATV AIMSYSGYDI 790 800 810 820 830 840 EDALVLNKSS IDRGFGRCQV FHKHSVIVRK YPNGTHDRIG DPQRDPETGE VVWKHGVVED 850 860 870 880 890 900 DGLAGVGCRV QPGQIYVNKQ TPTNALDNSI TLGHTQTVES GYKATPMTYK APEPGYIDKV 910 920 930 940 950 960 MLTTTDSDQT LIKVLMRQTR RPELGDKFSS RHGQKGVCGV IVQQEDMPFN DQGICPDIIM 970 980 990 1000 1010 1020 NPHGFPSRMT VGKMIELLSG KVGVLRGTLE YGTCFGGTKV EDASRILVEH GYNYSGKDML 1030 1040 1050 1060 1070 1080 TSGITGETLE AYIFMGPIYY QKLKHMVMDK MHARARGPRA VLTRQPTEGR SRDGGLRLGE 1090 1100 1110 1120 1130 1140 MERDCLIAYG ASQLLLERLM ISSDACDVDV CGQCGLLGYK GWCNSCQSTR EVVKMTIPYA 1150 1160 AKLLFQELLS MNIVPRLALE DEFKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III." Proshkina G.M., Shematorova E.K., Proshkin S.A., Zaros C., Thuriaux P., Shpakovski G.V. Nucleic Acids Res. 34:3615-3624(2006) [PubMed: 16877568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ156226 mRNA. Translation: ABA54854.1. CU329670 Genomic DNA. Translation: CAA93558.1.
PIR	T38867.
RefSeq	NP_593690.1.
3D structure databases
HSSP	HSSP built from PDB template 1I50 based on UniProtKB P08518.
ModBase	Search...
Genome annotation databases
GeneID	2543393.
KEGG	spo:SPAC4G9.08c.
NMPDR	fig|4896.1.peg.3660.
Organism-specific databases
GeneDB_Spombe	SPAC4G9.08c.
Phylogenomic databases
OMA	Q10233. FKMERAG.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001356-MON.
BRENDA	2.7.7.6. 653.
Gene expression databases
ArrayExpress	Q10233.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC2_SCHPO

Accession

Primary (citable) accession number: Q10233
Secondary accession number(s): Q0QYE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)