ID   ERG7_SCHPO              Reviewed;         721 AA.
AC   Q10231;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   05-MAY-2009, entry version 64.
DE   RecName: Full=Lanosterol synthase;
DE            EC=5.4.99.7;
DE   AltName: Full=Oxidosqualene--lanosterol cyclase;
DE            Short=OSC;
DE   AltName: Full=2,3-epoxysqualene--lanosterol cyclase;
GN   Name=erg7; ORFNames=SPAC13G7.01c, SPAC4G9.21c;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=96193650; PubMed=8604986; DOI=10.1006/bbrc.1996.0232;
RA   Corey E.J., Matsuda S.P.T., Baker C.H., Ting A.Y., Cheng H.;
RT   "Molecular cloning of a Schizosaccharomyces pombe cDNA encoding
RT   lanosterol synthase and investigation of conserved tryptophan
RT   residues.";
RL   Biochem. Biophys. Res. Commun. 219:327-331(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Catalyzes the cyclization of (S)-2,3 oxidosqualene to
CC       lanosterol, a reaction that forms the sterol nucleus.
CC   -!- CATALYTIC ACTIVITY: (S)-2,3-epoxysqualene = lanosterol.
CC   -!- PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol
CC       from farnesyl-PP: step 3/3.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC   -!- SIMILARITY: Contains 4 PFTB repeats.
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DR   EMBL; U41368; AAA92502.1; -; mRNA.
DR   EMBL; CU329670; CAA93571.1; -; Genomic_DNA.
DR   PIR; JC4643; JC4643.
DR   RefSeq; NP_593702.2; -.
DR   GeneID; 2542882; -.
DR   KEGG; spo:SPAC13G7.01c; -.
DR   GeneDB_Spombe; SPAC13G7.01c; -.
DR   BioCyc; SPOM-XXX-01:SPOM-XXX-01-002010-MON; -.
DR   BRENDA; 5.4.99.7; 653.
DR   ArrayExpress; Q10231; -.
DR   GO; GO:0005829; C:cytosol; IDA:GeneDB_SPombe.
DR   GO; GO:0000250; F:lanosterol synthase activity; IEA:EC.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001330; Prenyltrans.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   Pfam; PF00432; Prenyltrans; 3.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Isomerase; Lipid synthesis; Repeat;
KW   Steroid biosynthesis.
FT   CHAIN         1    721       Lanosterol synthase.
FT                                /FTId=PRO_0000072656.
FT   REPEAT      121    162       PFTB 1.
FT   REPEAT      555    595       PFTB 2.
FT   REPEAT      604    645       PFTB 3.
FT   REPEAT      662    703       PFTB 4.
FT   ACT_SITE    229    229       Proton acceptor (By similarity).
FT   ACT_SITE    451    451       Proton donor (By similarity).
SQ   SEQUENCE   721 AA;  82819 MW;  445B67F4BF7BFBA3 CRC64;
     MEACRVRPEL SKTVEQIDKS LWRLNIDSAG GETWEYVTKE EAEKRPLTIA EKYFLGFDLD
     LPKRPPAKTP LESAEYGYEF FRRLQLPDGH WASPYEGPMF LICGAVFAFY ISQTPFPKGW
     APEIIQYLIN HTNDDGGWGI HTEGVSTVFG TSMNYVVLRI LGMDAGHPVA TRARNRLHEL
     GGAIGCPHWG KFWLATLNCY DWDGVNPIPP ELWLLPDWIP FHPGKWWCHV RLVYLPMGYM
     YGERLKCPKD SLIMQLRKEL YVENYDSINF ADHRNTISDV DLYFPHTQIL DRLNWILEKY
     FTYLRPSWLK KLGTRRAYEL IKIEDQNTDY SCIGPVNAAM NTVCVYFHEG PSSKAFQKHI
     QRLHDFMWVQ PEGMLMRGTN GLQVWETSFT LQALVESGLY EKEAFKPDIA KALEFLDRQQ
     IRTQYEGSGY RYNSLGAWPF SNITQGYTVS DTTSEALRAV LLVQSLPDFE KLVDIPRLRL
     SVDVILGMQN ENLGFASYEP ARTGEWMELL NPAEVFGNIM VEYSYPECTT SVILALRAFT
     KYDPGYRRDE IENTIENALE YVVKMQRPDG SWYGSWAICF TYAAMFATGS LASAGRYYEN
     CPVQKKACEF LLSKQRPDGG WSESYMACVT GVYTETESSL VTQTGWALDA LINAKYPDRK
     PIEKGIKFLM ASQKSDGSWQ QKSMEGIFNK NVAIAYPNYK LYFSIYTLGK FAKQYGNYLT
     I
//