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Protein

Lanosterol synthase

Gene

erg7

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.

Pathwayi: lanosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (erg9)
  2. Probable squalene monooxygenase (erg1)
  3. Lanosterol synthase (erg7)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei229Proton acceptorBy similarity1
Active sitei451Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-191273. Cholesterol biosynthesis.
UniPathwayiUPA00767; UER00753.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol synthase (EC:5.4.99.7)
Alternative name(s):
2,3-epoxysqualene--lanosterol cyclase
Oxidosqualene--lanosterol cyclase
Short name:
OSC
Gene namesi
Name:erg7
ORF Names:SPAC13G7.01c, SPAC4G9.21c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC13G7.01c.
PomBaseiSPAC13G7.01c. erg7.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726561 – 721Lanosterol synthaseAdd BLAST721

Proteomic databases

MaxQBiQ10231.
PRIDEiQ10231.

Interactioni

Protein-protein interaction databases

MINTiMINT-4697960.

Structurei

3D structure databases

ProteinModelPortaliQ10231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati121 – 162PFTB 1Add BLAST42
Repeati555 – 595PFTB 2Add BLAST41
Repeati604 – 645PFTB 3Add BLAST42
Repeati662 – 703PFTB 4Add BLAST42

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000234317.
InParanoidiQ10231.
KOiK01852.
OMAiCAINRTS.
OrthoDBiEOG092C0KMX.
PhylomeDBiQ10231.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEACRVRPEL SKTVEQIDKS LWRLNIDSAG GETWEYVTKE EAEKRPLTIA
60 70 80 90 100
EKYFLGFDLD LPKRPPAKTP LESAEYGYEF FRRLQLPDGH WASPYEGPMF
110 120 130 140 150
LICGAVFAFY ISQTPFPKGW APEIIQYLIN HTNDDGGWGI HTEGVSTVFG
160 170 180 190 200
TSMNYVVLRI LGMDAGHPVA TRARNRLHEL GGAIGCPHWG KFWLATLNCY
210 220 230 240 250
DWDGVNPIPP ELWLLPDWIP FHPGKWWCHV RLVYLPMGYM YGERLKCPKD
260 270 280 290 300
SLIMQLRKEL YVENYDSINF ADHRNTISDV DLYFPHTQIL DRLNWILEKY
310 320 330 340 350
FTYLRPSWLK KLGTRRAYEL IKIEDQNTDY SCIGPVNAAM NTVCVYFHEG
360 370 380 390 400
PSSKAFQKHI QRLHDFMWVQ PEGMLMRGTN GLQVWETSFT LQALVESGLY
410 420 430 440 450
EKEAFKPDIA KALEFLDRQQ IRTQYEGSGY RYNSLGAWPF SNITQGYTVS
460 470 480 490 500
DTTSEALRAV LLVQSLPDFE KLVDIPRLRL SVDVILGMQN ENLGFASYEP
510 520 530 540 550
ARTGEWMELL NPAEVFGNIM VEYSYPECTT SVILALRAFT KYDPGYRRDE
560 570 580 590 600
IENTIENALE YVVKMQRPDG SWYGSWAICF TYAAMFATGS LASAGRYYEN
610 620 630 640 650
CPVQKKACEF LLSKQRPDGG WSESYMACVT GVYTETESSL VTQTGWALDA
660 670 680 690 700
LINAKYPDRK PIEKGIKFLM ASQKSDGSWQ QKSMEGIFNK NVAIAYPNYK
710 720
LYFSIYTLGK FAKQYGNYLT I
Length:721
Mass (Da):82,819
Last modified:October 1, 1996 - v1
Checksum:i445B67F4BF7BFBA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41368 mRNA. Translation: AAA92502.1.
CU329670 Genomic DNA. Translation: CAA93571.1.
PIRiJC4643.
RefSeqiNP_593702.2. NM_001019134.2.

Genome annotation databases

EnsemblFungiiSPAC13G7.01c.1; SPAC13G7.01c.1:pep; SPAC13G7.01c.
GeneIDi2542882.
KEGGispo:SPAC13G7.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41368 mRNA. Translation: AAA92502.1.
CU329670 Genomic DNA. Translation: CAA93571.1.
PIRiJC4643.
RefSeqiNP_593702.2. NM_001019134.2.

3D structure databases

ProteinModelPortaliQ10231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4697960.

Proteomic databases

MaxQBiQ10231.
PRIDEiQ10231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC13G7.01c.1; SPAC13G7.01c.1:pep; SPAC13G7.01c.
GeneIDi2542882.
KEGGispo:SPAC13G7.01c.

Organism-specific databases

EuPathDBiFungiDB:SPAC13G7.01c.
PomBaseiSPAC13G7.01c. erg7.

Phylogenomic databases

HOGENOMiHOG000234317.
InParanoidiQ10231.
KOiK01852.
OMAiCAINRTS.
OrthoDBiEOG092C0KMX.
PhylomeDBiQ10231.

Enzyme and pathway databases

UniPathwayiUPA00767; UER00753.
ReactomeiR-SPO-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiQ10231.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERG7_SCHPO
AccessioniPrimary (citable) accession number: Q10231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.