Q10223 (POF14_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein pof14

Gene names

Name:	pof14
ORF Names:	SPAC13D6.01

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Expression is induced during oxidative stress. Plays an essential, SCF-independent, role in the stress response to hydrogen peroxide for survival, by negatively regulating ergosterol synthesis via direct binding to the squalene synthase erg9. Ref.2
Subunit structure	Component of the E3 ubiquitin ligase Skp1-Cullin-1-F-box (SCF) complex. Interacts with skp1, cul1 and erg9. Ref.2
Subcellular location	Cytoplasm. Nucleus. Endoplasmic reticulum. Note: Associated with vesicle-like and endoplasmic reticulum (excluding a nuclear rim) structures. Ref.2 Ref.3
Induction	Expression is induced during oxidative stress. Ref.2
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Endoplasmic reticulum Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to oxidative stress Inferred from mutant phenotype. Source: GeneDB_Spombe protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from physical interaction. Source: GeneDB_Spombe regulation of ergosterol biosynthetic process Inferred from direct assay Ref.2. Source: UniProtKB
Cellular component	SCF ubiquitin ligase complex Inferred from direct assay Ref.2. Source: UniProtKB cytosol Inferred from direct assay. Source: GeneDB_Spombe endoplasmic reticulum membrane Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	protein binding Inferred from physical interaction Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
erg9	P36596	5	EBI-1793014,EBI-1794119
skp1	Q9Y709	5	EBI-1793014,EBI-1172248

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	F-box protein pof14		PRO_0000116488
Regions
	Domain	172 – 186	15	F-box; atypical

Sequences

	Sequence		Length	Mass (Da)
	Q10223 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 132FB0919646AC9F	FASTA	431	49,624
	10 20 30 40 50 60 MLQFSFQNCK THTCYMNTSV NEHLDQDESF LRILIDTRKK IRSSYFKPQT FDEFVHCLAR 70 80 90 100 110 120 VRAMKRLVSI CSNFDEEDNW NGVWNTLVVD GDSHASAMID YEGLLDKCSL SRNLLELIND 130 140 150 160 170 180 YAEYTTQVLP FRKIPSSDNL DSSLNLTVAS PKSNLYYRYS SESPKYAKIL DCPDEILQLI 190 200 210 220 230 240 FSYCYDASYI EKLPFAFSYR KQRHTLIHDL PNTCLRFKKI LSPRNVSFWK RLLKVHKKNP 250 260 270 280 290 300 NSAVTCSESI NTSAVAKFTD IPTIIPIFLD PSYQSYAAKT IHSDTSSLAS SIIQTGDGTQ 310 320 330 340 350 360 SCDESTYIEL ACNLVGRCVL CNRIPKLTKF KDCITSFYRP SVATNICDQC LEAIIDFYEP 370 380 390 400 410 420 VSRYKFDVMK DRLGVGYISR PGQKFPSWLS EHVQLARDEE KAFKSIYHSQ GEFARSLFRL 430 FRAQLAELCE Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF." Tafforeau L., Le Blastier S., Bamps S., Dewez M., Vandenhaute J., Hermand D. EMBO J. 25:4547-4556(2006) [PubMed: 17016471] [Abstract] Cited for: FUNCTION, INTERACTION WITH CUL1; ERG9 AND SKP1, SUBCELLULAR LOCATION, INDUCTION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA93541.1.
PIR	T37621.
RefSeq	NP_593679.1. NM_001019111.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q10223. 6 interactions.
MINT	MINT-3376933.
STRING	Q10223.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC13D6.01.1; SPAC13D6.01.1:pep; SPAC13D6.01.
GeneID	2542815.
GenomeReviews	Gene locus pof14 in contig CU329670_GR.
KEGG	spo:SPAC13D6.01.
NMPDR	fig|4896.1.peg.3649.
Organism-specific databases
GeneDB_Spombe	SPAC13D6.01.
Phylogenomic databases
OrthoDB	EOG4V9Z1V.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001346-MONOMER.
Gene expression databases
ArrayExpress	Q10223.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POF14_SCHPO

Accession

Primary (citable) accession number: Q10223

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program