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Protein

ATP-dependent DNA helicase srs2

Gene

srs2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase involved in DNA repair at least for UV-induced lesions. Also aids the recombinational repair of camptothecin-induced collapsed replication forks.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei302 – 3021ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to UV Source: PomBase
  • postreplication repair Source: PomBase
  • recombinational repair Source: PomBase
  • replication fork processing Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase srs2 (EC:3.6.4.12)
Gene namesi
Name:srs2
ORF Names:SPAC4H3.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4H3.05.
PomBaseiSPAC4H3.05. srs2.

Subcellular locationi

GO - Cellular componenti

  • mitotic spindle Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887ATP-dependent DNA helicase srs2PRO_0000102083Add
BLAST

Proteomic databases

MaxQBiQ10213.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
sws1O136002EBI-8527058,EBI-8527040

Protein-protein interaction databases

BioGridi279992. 58 interactions.
IntActiQ10213. 1 interaction.
MINTiMINT-2730223.

Structurei

3D structure databases

ProteinModelPortaliQ10213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 304296UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini305 – 597293UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ10213.
KOiK03657.
OMAiRLQIAFQ.
OrthoDBiEOG77HDP4.
PhylomeDBiQ10213.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 2 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METKSSYLKF LNEEQRISVQ SPHKYTQILA GPGSGKTRVL TARVAYLLQK
60 70 80 90 100
NHIAAEDLII ATFTNKAANE IKLRIEAILG NSEASKLISG TFHSIAYKYL
110 120 130 140 150
VKYGKHIGLS SNWLIADRND TQAIMKRLLD SLKKAKNPIA SGIRGQELTP
160 170 180 190 200
QNALNRITKL KSNGLLVKPG MDQLSLINGL EEPPKELQSH QSVELYRLYQ
210 220 230 240 250
TSLWKNNLAD FDDLLLNFIL LLQKQPDCVR NIKHILIDEF QDTSKIQYFL
260 270 280 290 300
VKLLALQNSD ITIVGDPDQS IYGFRSAEIR NLNQMSEDFE GTQVLHLERN
310 320 330 340 350
YRSAKPILEL ALSIISQDKS RPKKGLKSNH ISSLKPHYRL FETNNKESYW
360 370 380 390 400
IAREIKRIVG SCPELIFYND IAILVRSSSL TRSLEHALSE LGVPYRMVGV
410 420 430 440 450
NKFFDREEIR DLIAYLRVLA NKDSTSLIRV INVPPRNIGK TKIDRIIFES
460 470 480 490 500
ERRGLTFWQT LNEVKNENIL LSQRNDKSFL KSLKSFLCSI SKLENRYLSN
510 520 530 540 550
GHSATLSDLL LGILSEIKYY EYLVRKNKET VEEKWENVME LVQQSDNISC
560 570 580 590 600
IFYELDYKIS TIVLLQNFLT QIALVNEEQK EGESQKVTIS TLHAAKGLEW
610 620 630 640 650
PVVFLPCLCE NIIPHSRSDD LDEERRLLYV GATRAQALLY LSSFKSVTGM
660 670 680 690 700
FADMQNSDNV QDVSPFLKGE EMKRWVMESE IVFNEKIASE IGTILGRKSY
710 720 730 740 750
GKITNLSGIG SNANHNGTKF ENLGFQCCRV LAEAELKKRE RVKSVNDYNK
760 770 780 790 800
DETNFRKHNA KRSKTDIRSW FEKKQPIDSD VEISEPSRSA SIMVANKDLN
810 820 830 840 850
DRSFETVNRI VSTRASTTNA SFMSSVRQNL GRGPSTKDQV INRTLREGHQ
860 870 880
DVVQHTDLNQ SNTKVASARP AGSRKRLGVR LRVSRML
Length:887
Mass (Da):101,124
Last modified:October 1, 1996 - v1
Checksum:i39830861766783F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93344.1.
PIRiT38885.
RefSeqiNP_594341.1. NM_001019762.2.

Genome annotation databases

EnsemblFungiiSPAC4H3.05.1; SPAC4H3.05.1:pep; SPAC4H3.05.
GeneIDi2543577.
KEGGispo:SPAC4H3.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93344.1.
PIRiT38885.
RefSeqiNP_594341.1. NM_001019762.2.

3D structure databases

ProteinModelPortaliQ10213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279992. 58 interactions.
IntActiQ10213. 1 interaction.
MINTiMINT-2730223.

Proteomic databases

MaxQBiQ10213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4H3.05.1; SPAC4H3.05.1:pep; SPAC4H3.05.
GeneIDi2543577.
KEGGispo:SPAC4H3.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC4H3.05.
PomBaseiSPAC4H3.05. srs2.

Phylogenomic databases

InParanoidiQ10213.
KOiK03657.
OMAiRLQIAFQ.
OrthoDBiEOG77HDP4.
PhylomeDBiQ10213.

Miscellaneous databases

PROiQ10213.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 2 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast."
    Doe C.L., Whitby M.C.
    Nucleic Acids Res. 32:1480-1491(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSRS2_SCHPO
AccessioniPrimary (citable) accession number: Q10213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.