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Q10208

- KPYK_SCHPO

UniProt

Q10208 - KPYK_SCHPO

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Protein

Pyruvate kinase

Gene
pyk1, SPAC4H3.10c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561Substrate By similarity
Metal bindingi58 – 581Potassium By similarity
Metal bindingi60 – 601Potassium By similarity
Metal bindingi91 – 911Potassium By similarity
Metal bindingi92 – 921Potassium; via carbonyl oxygen By similarity
Sitei247 – 2471Transition state stabilizer By similarity
Metal bindingi249 – 2491Magnesium Reviewed prediction
Binding sitei272 – 2721Substrate; via amide nitrogen By similarity
Metal bindingi273 – 2731Magnesium By similarity
Binding sitei273 – 2731Substrate; via amide nitrogen By similarity
Binding sitei305 – 3051Substrate By similarity
Binding sitei344 – 3441ADP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: PomBase

GO - Biological processi

  1. cellular response to nitrogen starvation Source: PomBase
  2. glycolytic process Source: PomBase
  3. negative regulation of G0 to G1 transition Source: PomBase
  4. pyruvate metabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BRENDAi2.7.1.40. 5615.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk1
ORF Names:SPAC4H3.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC4H3.10c.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Pyruvate kinasePRO_0000112118Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Phosphoserine1 Publication
Modified residuei63 – 631Phosphoserine1 Publication
Modified residuei281 – 2811Phosphoserine1 Publication
Modified residuei412 – 4121Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10208.
PaxDbiQ10208.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

BioGridi279974. 8 interactions.
MINTiMINT-4697816.
STRINGi4896.SPAC4H3.10c-1.

Structurei

3D structure databases

ProteinModelPortaliQ10208.
SMRiQ10208. Positions 26-506.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
KOiK00873.
OMAiCIVENAG.
OrthoDBiEOG7M6DJC.
PhylomeDBiQ10208.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10208-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSAVSPKQ WVAGLNSELD IPAVNRRTSI ICTIGPKSNN VETLCKLRDA    50
GMNIVRMNFS HGSYEYHQSV IDNARKASAT NPLFPLAIAL DTKGPEIRTG 100
LTVGGTDYPI SSGHEMIFTT DDAYAEKCND KVMYIDYKNI TKVIQPGRII 150
YVDDGILSFT VIEKVDDKNL KVRVNNNGKI SSKKGVNLPK TDVDLPALSE 200
KDKADLRFGV KNGVDMIFAS FIRRAEDVIH IREVLGEEGK NIKIICKIEN 250
QQGVNNFDSI LDVTDGIMVA RGDLGIEIPA SQVFVAQKMM IAKCNIAGKP 300
VACATQMLES MTYNPRPTRA EVSDVGNAVL DGADLVMLSG ETTKGSYPVE 350
AVTYMAETAR VAEASIPYGS LYQEMFGLVR RPLECATETT AVAAIGASIE 400
SDAKAIVVLS TSGNTARLCS KYRPSIPIVM VTRCPQRARQ SHLNRGVYPV 450
IYEKEPLSDW QKDVDARVAY GCQQAYKMNI LKKGDKIIVL QGAVGGKGHT 500
SIFRLTVAE 509
Length:509
Mass (Da):55,515
Last modified:October 1, 1996 - v1
Checksum:i589378533FCFD5F7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti391 – 3911A → R in CAA62490. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91008 mRNA. Translation: CAA62490.1.
CU329670 Genomic DNA. Translation: CAA93349.1.
PIRiT38890.
T45166.
RefSeqiNP_594346.1. NM_001019767.2.

Genome annotation databases

EnsemblFungiiSPAC4H3.10c.1; SPAC4H3.10c.1:pep; SPAC4H3.10c.
GeneIDi2543557.
KEGGispo:SPAC4H3.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91008 mRNA. Translation: CAA62490.1 .
CU329670 Genomic DNA. Translation: CAA93349.1 .
PIRi T38890.
T45166.
RefSeqi NP_594346.1. NM_001019767.2.

3D structure databases

ProteinModelPortali Q10208.
SMRi Q10208. Positions 26-506.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279974. 8 interactions.
MINTi MINT-4697816.
STRINGi 4896.SPAC4H3.10c-1.

Proteomic databases

MaxQBi Q10208.
PaxDbi Q10208.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC4H3.10c.1 ; SPAC4H3.10c.1:pep ; SPAC4H3.10c .
GeneIDi 2543557.
KEGGi spo:SPAC4H3.10c.

Organism-specific databases

PomBasei SPAC4H3.10c.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
KOi K00873.
OMAi CIVENAG.
OrthoDBi EOG7M6DJC.
PhylomeDBi Q10208.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BRENDAi 2.7.1.40. 5615.

Miscellaneous databases

NextBioi 20804566.
PROi Q10208.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a gene encoding pyruvate kinase from Schizosaccharomyces pombe; implications for quaternary structure and regulation of the enzyme."
    Nairn J., Smith S., Allison P.J., Rigden D., Fothergill-Gilmore L.A., Price N.C.
    FEMS Microbiol. Lett. 134:221-226(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-63; SER-281 AND SER-412, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiKPYK_SCHPO
AccessioniPrimary (citable) accession number: Q10208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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