Reviewed, UniProtKB/Swiss-Prot Q10197 (ALP1_SCHPO)

Names and origin

Protein names

Recommended name:
    Tubulin-folding cofactor D
Alternative name(s):
    Altered polarity protein 1

Gene names

Name:	alp1
ORF Names:	SPBC11C11.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a function in the folding of beta-tubulin. Microtubule-associated protein that is essential to direct polarized cell growth and to position the nucleus and septum to the center of the cell during mitosis. Ref.1
Subunit structure	Interacts with alp21. Ref.3
Subcellular location	Cytoplasm › cytoskeleton. Note: Microtubule-associated. Ref.1
Sequence similarities	Contains 1 HEAT repeat.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Molecular function	Chaperone
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	establishment or maintenance of cell polarity Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe microtubule cytoskeleton organization Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe protein folding Ref.1 Traceable author statement. Source: GeneDB_SPombe
Cellular component	astral microtubule Ref.1 Inferred from direct assay. Source: GeneDB_SPombe cell division site Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1121	1121	Tubulin-folding cofactor D		PRO_0000064568
Regions
	Repeat	313 – 351	39	HEAT
Amino acid modifications
	Glycosylation	122	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	721	1	N-linked (GlcNAc...) Potential
	Glycosylation	885	1	N-linked (GlcNAc...) Potential
	Glycosylation	1099	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	988 – 1001	14	Missing in CAA20686. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q10197-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: DCF045E3B81213A2	FASTA	1,121	128,187
	10 20 30 40 50 60 MEEEESLEGI ISIDESLITS RLSQILDDVL DDRSSSHSVE KLKDVAVKYL QFCQFQPTLL 70 80 90 100 110 120 DKLLSKYVPN LASYLLKVKN IGKCNSITVI LYQFCKIRGY KAVRVLFPVG VQYIKELYTL 130 140 150 160 170 180 LNESSNNTWH FHYIVLLWLS QALNTPFPLN SLDDSLDVKK TIYTIAIKYL ENSGIDKEAS 190 200 210 220 230 240 CLVLSRLFSR DDGLDLLLGF LHHCESSWFK RSIFYKIGCL FSLSSFLKIC PRNDCLQTVD 250 260 270 280 290 300 VAFQFLNVAR EDLVGQENSA LRKLLCKCYT RLGIVLLPVN SSPNWKYSIS NPDSFFQLPD 310 320 330 340 350 360 DSNEEVHIYL EVIVDFLLSS VSDIDSFVRW SAAKGLAKII SRLPWNLAEQ VIDAIIELMT 370 380 390 400 410 420 ENMFLNPIEN TVNISITSPL VWHGAILFFA KLAGAGLIKY SKCLHILPLI EVGLSYEVRY 430 440 450 460 470 480 GTRVTGQSIR DASCYFVWSF YHCYSKSAIE GLQTNLILCL LQTVLFDNEI NVRRAATAAL 490 500 510 520 530 540 FEVIGRHASI PDGLSLISHI NYVSVTDISN CYGDLCMKVA HFPQFRSCVF QRLFTNLQHW 550 560 570 580 590 600 DVKVQQLSAF SLRQLSIKYP KELSIYLPPI LDYLSVGNAD FIFGYTIGLA SIIGGFLSIS 610 620 630 640 650 660 FPFDINRIHD LLSHKNLLSL KKFSRQQQTK IILGILKGIQ QIFANDIRVD RAFFSEAFSV 670 680 690 700 710 720 IIAAIDLQEE TIIKDISDAY SVLVKFDDME ETLEVLLDYI RKCSTSKEAR IVYIILQNLP 730 740 750 760 770 780 NISFRYQKKI CKLLLDIYPQ LHSIDYQAPV ANALQNIIPF TYEKTESIEE FVKELLQVCS 790 800 810 820 830 840 NYLTDTRGDV GSWIRKPAMK AISSLLVKDS SGKKLSEDIV WCCISYIIRQ TFDKIDSLRG 850 860 870 880 890 900 LAYQALEQIR VHYLIRRCEA LTNIINRISF RNNPNMDGEV LNELNISLLE IPNLRLQAFY 910 920 930 940 950 960 GITVFTADGF GSDLAVKCFE FYLSYVYQLE DSFKKSNSRY GKRDLLQLYI DILSSEDEIA 970 980 990 1000 1010 1020 RFYFPIMKSF TSLLAYGCFT DFQNVKGYVF LHFVQNTNDI SMSKAIFIVQ RRALTCKSPG 1030 1040 1050 1060 1070 1080 GLSAILELYR TLFLSKNELL RHHALKYTAN LLLNPIEKVR YQAADTLLYA KSIGLLTFLP 1090 1100 1110 1120 NELNQKLLTL DWFVPVSQNA TFVKQLRNII QKQIDKLIAD R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Essential role of tubulin-folding cofactor D in microtubule assembly and its association with microtubules in fission yeast." Hirata D., Masuda H., Eddison M., Toda T. EMBO J. 17:658-666(1998) [PubMed: 9450991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: 972 / HM123.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast." Radcliffe P.A., Hirata D., Vardy L., Toda T. Mol. Biol. Cell 10:2987-3001(1999) [PubMed: 10473641] [Abstract] Cited for: INTERACTION WITH ALP21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y10106 Genomic DNA. Translation: CAA71193.1. CU329671 Genomic DNA. Translation: CAA20686.1.
PIR	S67381. T39319.
RefSeq	NP_596393.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q10197.
Genome annotation databases
GeneID	2539669.
GenomeReviews	Gene locus alp1 in contig CU329671_GR.
KEGG	spo:SPBC11C11.04c.
Organism-specific databases
GeneDB_Spombe	SPBC11C11.04c.
Phylogenomic databases
OrthoDB	EOG9Z6454
Gene expression databases
ArrayExpress	Q10197.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR000357. HEAT. [Graphical view]
Pfam	PF02985. HEAT. 1 hit. [Graphical view]
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALP1_SCHPO

Accession

Primary (citable) accession number: Q10197
Secondary accession number(s): P78749

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1999
Last modified:	November 24, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)