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Q10185 (ABC2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette transporter abc2
Short name=ABC transporter abc2
EC=3.6.3.-
Alternative name(s):
ATP-energized glutathione S-conjugate pump abc2
Glutathione S-conjugate-transporting ATPase abc2
Gene names
Name:abc2
ORF Names:SPAC3F10.11c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length1478 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in vacuolar sequestration of glutathione S-conjugates. Together with abc4, required for accumulation of a red pigment (ade pigment) in the vacuole of a mutant affected in the adenine biosynthetic pathway. Ref.2

Subcellular location

Vacuole membrane; Multi-pass membrane protein Potential Ref.2.

Disruption phenotype

Cells lacking both abc2 and abc4 show sensitivity to cycloheximide (CHX) and 4-nitroquinoline oxide (4-NQO), and decreased accumulation of monochlorobimane-glutathione (MCIB-GS). Ref.2

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14781478ATP-binding cassette transporter abc2
PRO_0000093468

Regions

Topological domain1 – 2525Vacuolar By similarity
Transmembrane26 – 4621Helical; Name=1; By similarity
Topological domain47 – 6519Cytoplasmic By similarity
Transmembrane66 – 8520Helical; Name=2; By similarity
Topological domain86 – 905Vacuolar By similarity
Transmembrane91 – 10414Helical; Name=3; By similarity
Topological domain105 – 11612Cytoplasmic By similarity
Transmembrane117 – 13721Helical; Name=4; By similarity
Topological domain138 – 15417Vacuolar By similarity
Transmembrane155 – 17521Helical; Name=5; By similarity
Topological domain176 – 25984Cytoplasmic By similarity
Transmembrane260 – 28021Helical; Name=6; By similarity
Topological domain281 – 31030Vacuolar By similarity
Transmembrane311 – 33121Helical; Name=7; By similarity
Topological domain332 – 38756Cytoplasmic By similarity
Transmembrane388 – 40821Helical; Name=8; By similarity
Topological domain409 – 4113Vacuolar By similarity
Transmembrane412 – 43221Helical; Name=9; By similarity
Topological domain433 – 49563Cytoplasmic By similarity
Transmembrane496 – 51621Helical; Name=10; By similarity
Topological domain517 – 53923Vacuolar By similarity
Transmembrane540 – 56021Helical; Name=11; By similarity
Topological domain561 – 910350Cytoplasmic By similarity
Transmembrane911 – 93121Helical; Name=12; By similarity
Topological domain932 – 96837Vacuolar By similarity
Transmembrane969 – 99022Helical; Name=13; By similarity
Topological domain991 – 103343Cytoplasmic By similarity
Transmembrane1034 – 105421Helical; Name=14; By similarity
Topological domain10551Vacuolar By similarity
Transmembrane1056 – 107621Helical; Name=15; By similarity
Topological domain1077 – 114771Cytoplasmic By similarity
Transmembrane1148 – 116821Helical; Name=16; By similarity
Topological domain1169 – 11724Vacuolar By similarity
Transmembrane1173 – 119321Helical; Name=17; By similarity
Topological domain1194 – 1478285Cytoplasmic By similarity
Domain268 – 557290ABC transmembrane type-1 1
Domain593 – 821229ABC transporter 1
Domain918 – 1202285ABC transmembrane type-1 2
Domain1239 – 1473235ABC transporter 2
Nucleotide binding631 – 6388ATP 1 Potential
Nucleotide binding1273 – 12808ATP 2 Potential

Amino acid modifications

Modified residue8391Phosphoserine Ref.3
Modified residue8431Phosphoserine Ref.3
Modified residue8631Phosphoserine Ref.3
Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation1391N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q10185 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6C59F43105EB7187

FASTA1,478166,938
        10         20         30         40         50         60 
MVLEQDLDPF VGGNWMNSAY KGFTFLSATW LAPNIYLLIS GCLQYFYEVR KRSHYFHFRR 

        70         80         90        100        110        120 
FWTIWLKSLV IMVLLFTHIY DCYKTNESVW NVLSIITYFL ALFLHVVEQP TLRIPMASLL 

       130        140        150        160        170        180 
MFWLFKFLAS ALVLLLRPNY TMFPMLNVVP SITFFCSLVC LLAEIYVPPA NRVWYPDDAA 

       190        200        210        220        230        240 
ELEETGLRPS RFTYANIFSR ISFGWLSPLM KFGYRNYLTE SDAWSLPPAE RSSNLTIVFE 

       250        260        270        280        290        300 
KNWISHAKKK KSSLYMWGVL FLNHWKLTVV IIVLKLVQDV VAFIQPNLIR KIVIFVSSYS 

       310        320        330        340        350        360 
SEHPQPPQVG FSLAIAMFLT NVVQTALLQQ YFQLGMVLGM RWRSELITAI YRKSLRLSSA 

       370        380        390        400        410        420 
ARQSRSVGDI VNYMSVDTQK VCDLTMFLFV IVSGPFQIVL ALTNLYHLVG YGALSGAFVT 

       430        440        450        460        470        480 
FLLFPCNVVI ASIFKRFQNR QMKNKDARSQ FMTEIINNIR SIKLYAWENI FLQKLLQLRN 

       490        500        510        520        530        540 
TRELRMLKKI GIVNTIGNFT WLFAPILVSA ATFGTFIVLY GKTRVLSVDI VFACLSLFNL 

       550        560        570        580        590        600 
LQFPLTMLPI VVSSVLEASV AISRIYGFLT AGELDSNAVQ RYPANKEPSG VCLEIKKGTF 

       610        620        630        640        650        660 
SWSGPGQNAA EPTLRDIDFV ARRGELCCIV GKVGMGKSSL LEACLGNMQK HSGSVFRCGS 

       670        680        690        700        710        720 
IAYAAQQPWI LNATIQENIL FGLELDPEFY EKTIRACCLL RDFEILADGD QTEVGEKGIS 

       730        740        750        760        770        780 
LSGGQKARIS LARAVYSRSD IYLLDDILSA VDQHVNRDLV RNLLGSKGLL RSRCVILSTN 

       790        800        810        820        830        840 
SLTVLKEASM IYMLRNGKII ESGSFTQLSS SPDSQLFQLL SEFSKKDTAS STGADTPLSR 

       850        860        870        880        890        900 
SQSVITSSTD VTSSASRSSD TVSNYPKATI KGTGRIRKRL TDEDNVKATG QAAEKMERGK 

       910        920        930        940        950        960 
VKWKVYWTYF KACSLFLIFL YFLFIIGGIG MNVGTNVWLK HWSEVNTQLG YNPKPYFYLG 

       970        980        990       1000       1010       1020 
IYTLFGLLSC ALISLSSLTI TVFCAIKSCR YLHDSMVKAV LRAPMSFFET TPTGRILNRF 

      1030       1040       1050       1060       1070       1080 
SSDVYRVDEV ISRVFMFFFR NLFQIVFVLA VICYSSPMFM ILIVPLFFLY RYNQVYYTQT 

      1090       1100       1110       1120       1130       1140 
SRELKRLDSV TRSPLYAHFQ ESLGGLSTIR AYDMEDTFIS ENDIRVDTNH RIWFLYFSSN 

      1150       1160       1170       1180       1190       1200 
RWQAIRVEAI GALVVFSSAF FGVLSAVRGN PNSGLVGLSL SYAVQITQSL TFVVRQSVDV 

      1210       1220       1230       1240       1250       1260 
ETNIVSVERM LEYIGLPSEA PSIIPDHRPP EGWPSHGAIK FDHYSVRYRE NLPLVLNDIS 

      1270       1280       1290       1300       1310       1320 
VNIKPQEKIG IVGRTGAGKS TLTLALFRLI EPTSGDIQLD DINITSIGLH DLRSRLAIIP 

      1330       1340       1350       1360       1370       1380 
QENQAFEGTI RENLDPNANA TDEEIWHALE AASLKQFIQT LDGGLYSRVT EGGANLSSGQ 

      1390       1400       1410       1420       1430       1440 
RQLMCLTRAL LTPTRVLLLD EATAAVDVET DAIVQRTIRE RFNDRTILTI AHRINTVMDS 

      1450       1460       1470 
NRILVLDHGK VVEFDSTKKL LENKASLFYS LAKESGLI

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant."
Iwaki T., Giga-Hama Y., Takegawa K.
Microbiology 152:2309-2321(2006) [PubMed: 16849797] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[3]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-839; SER-843 AND SER-863, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329670 Genomic DNA. Translation: CAA93309.1.
PIRT38712.

3D structure databases

ProteinModelPortalQ10185.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ10185.

Protein family/group databases

TCDB3.A.1.208.16. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC3F10.11c.1; SPAC3F10.11c.1:pep; SPAC3F10.11c.
GenomeReviewsGene locus abc2 in contig CU329670_GR.
NMPDRfig|4896.1.peg.3913.

Organism-specific databases

GeneDB_SpombeSPAC3F10.11c.

Phylogenomic databases

GeneTreeEFGT00050000001686.
HOGENOMHBG758042.
OrthoDBEOG4MSH69.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-001584-MONOMER.

Gene expression databases

ArrayExpressQ10185.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABC2_SCHPO
AccessionPrimary (citable) accession number: Q10185
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents