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Protein

Putative glycine--tRNA ligase

Gene

grs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs (By similarity).By similarity

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateBy similarity
Binding sitei221 – 2211SubstrateBy similarity
Binding sitei358 – 3581SubstrateBy similarity
Binding sitei358 – 3581Substrate; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi253 – 2553ATPBy similarity
Nucleotide bindingi263 – 2686ATPBy similarity
Nucleotide bindingi380 – 3812ATPBy similarity
Nucleotide bindingi503 – 5064ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: PomBase
  • diadenosine tetraphosphate biosynthetic process Source: UniProtKB
  • glycyl-tRNA aminoacylation Source: PomBase
  • mitochondrial translation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glycine--tRNA ligase (EC:6.1.1.14)
Alternative name(s):
Diadenosine tetraphosphate synthetase
Short name:
AP-4-A synthetase
Glycyl-tRNA synthetase
Short name:
GlyRS
Gene namesi
Name:grs1
ORF Names:SPAC3F10.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3F10.03.
PomBaseiSPAC3F10.03. grs1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 652652Putative glycine--tRNA ligasePRO_0000073004Add
BLAST

Proteomic databases

MaxQBiQ10179.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279611. 2 interactions.
MINTiMINT-4697545.

Structurei

3D structure databases

ProteinModelPortaliQ10179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 2725Substrate bindingBy similarity
Regioni499 – 5035Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000242015.
InParanoidiQ10179.
KOiK01880.
OMAiIEFRKRV.
OrthoDBiEOG76TB17.
PhylomeDBiQ10179.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVSKAAAF DRTQFEELMK KRFFFSPSFQ IYGGISGLYD YGPPGSALQS
60 70 80 90 100
NLVDIWRKHF VIEESMLEVD CSMLTPHEVL KTSGHVDKFA DWMCKDPATG
110 120 130 140 150
EIFRADHLVE EVLEARLKGD KEARGQNSND QPEESDDKKK RKKKVKEIRA
160 170 180 190 200
TRLDDKTVEE YEFILAQIDN YDGDQLGELM KKYDIRNPAT NGELETPRQF
210 220 230 240 250
NLMFETQIGP SGGLKGYLRP ETAQGQFLNF SRLLEFNNGK VPFASAMVGK
260 270 280 290 300
AFRNEISPRS GLLRVREFLM AEVEHFVDPK NKEHDRFDEV SHMPLRLLPR
310 320 330 340 350
GVQLEGKTDI LEMPIGDAVK KGIVDNTTLG YFMARISLFL EKIGIDMNRV
360 370 380 390 400
RFRQHMSNEM AHYACDCWDA EIQCSYGWIE CVGCADRSAY DLSVHSKATK
410 420 430 440 450
TPLVVQEALP EPVVVEQFEV EVNRKKFGPR FKRDAKAVEE AMISWPESEK
460 470 480 490 500
VEKSAQLVAE GKIIVNVNGV EHTVESDLVT IEKRKHTEHI RTYTPNVIEP
510 520 530 540 550
SFGLGRILYV LMEHAYWTRP EDVNRGVLSF PASIAPIKAL IVPLSRNAEF
560 570 580 590 600
APFVKKLSAK LRNLGISNKI DDSNANIGRR YARNDELGTP FGLTVDFETL
610 620 630 640 650
QNETITLRER DSTKQVRGSQ DEVIAALVSM VEGKSSFEDA LAKFGEFKST

QE
Length:652
Mass (Da):74,005
Last modified:October 1, 1996 - v1
Checksum:i3B7041D0616D2527
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93301.1.
PIRiT38704.
RefSeqiNP_593935.1. NM_001019363.2.

Genome annotation databases

EnsemblFungiiSPAC3F10.03.1; SPAC3F10.03.1:pep; SPAC3F10.03.
GeneIDi2543181.
KEGGispo:SPAC3F10.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93301.1.
PIRiT38704.
RefSeqiNP_593935.1. NM_001019363.2.

3D structure databases

ProteinModelPortaliQ10179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279611. 2 interactions.
MINTiMINT-4697545.

Proteomic databases

MaxQBiQ10179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3F10.03.1; SPAC3F10.03.1:pep; SPAC3F10.03.
GeneIDi2543181.
KEGGispo:SPAC3F10.03.

Organism-specific databases

EuPathDBiFungiDB:SPAC3F10.03.
PomBaseiSPAC3F10.03. grs1.

Phylogenomic databases

HOGENOMiHOG000242015.
InParanoidiQ10179.
KOiK01880.
OMAiIEFRKRV.
OrthoDBiEOG76TB17.
PhylomeDBiQ10179.

Miscellaneous databases

PROiQ10179.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYG_SCHPO
AccessioniPrimary (citable) accession number: Q10179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.