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Protein

Kinetochore protein nuf2

Gene

nuf2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.2 Publications

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: PomBase
  • cell division Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein nuf2
Alternative name(s):
NMS complex subunit nuf2
Gene namesi
Name:nuf2
ORF Names:SPAC27F1.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC27F1.04c.
PomBaseiSPAC27F1.04c. nuf2.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: PomBase
  • condensed nuclear chromosome kinetochore Source: PomBase
  • cytoplasm Source: UniProtKB-KW
  • meiotic spindle pole body Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • Ndc80 complex Source: PomBase
  • NMS complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579941 – 441Kinetochore protein nuf2Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143Phosphoserine1 Publication1
Modified residuei242Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10173.
PRIDEiQ10173.

PTM databases

iPTMnetiQ10173.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of ndc80, nuf2, spc24 and spc25. Can self-associate. Component of the NMS super complex which consists of mis12, mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7, spc24 and spc25.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ndc80Q101983EBI-1002565,EBI-1002524

Protein-protein interaction databases

BioGridi278487. 17 interactors.
DIPiDIP-36501N.
IntActiQ10173. 7 interactors.
MINTiMINT-4697479.

Structurei

3D structure databases

ProteinModelPortaliQ10173.
SMRiQ10173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili51 – 80Sequence analysisAdd BLAST30
Coiled coili128 – 186Sequence analysisAdd BLAST59
Coiled coili316 – 426Sequence analysisAdd BLAST111

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiQ10173.
KOiK11548.
OMAiQIFEWFA.
OrthoDBiEOG092C2OAH.
PhylomeDBiQ10173.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKHTFPSL KRAEILECID GLGIPFTAKE LDQPTSKAVI PLYEEFLDLF
60 70 80 90 100
MGLTRQNLEE KVNSLQDSVE NFEIIHESLR FTVFYQILSQ FMQNICFHDF
110 120 130 140 150
TIQDLLKPDR NRLQLILSAV INFAKLREER LQQFDDDIQK RESLLETYTL
160 170 180 190 200
LDAQRKDLEE KVLLSQDRKL ESEAIIKQNE ERNEEMFQSL IEDKRLCSQV
210 220 230 240 250
RTEYDRIRME ASELKIRYHN VDSLMASTLE EIEKLQSSIV HSPEKLKGKI
260 270 280 290 300
ADTSLRIQND RSQQVELDKK SKILHTKLNS LQLIEGDLNA CLKVLEECLV
310 320 330 340 350
ELDKLEHATV LLSTNQELCD QIEINKKKLE FRKEQLLKQL SNAQEKLEHE
360 370 380 390 400
QHSRNQKLEA AKQRMDNIRE EYKVITQERN KKIQETEKKN AMIEMTEQKI
410 420 430 440
AGMREELESQ ISSITMEFEK LKSHVELYIA ELLRNLRSSN S
Length:441
Mass (Da):51,911
Last modified:October 1, 1996 - v1
Checksum:iD716FE83223BD16E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93293.1.
PIRiT38462.
RefSeqiNP_594532.1. NM_001019961.2.

Genome annotation databases

EnsemblFungiiSPAC27F1.04c.1; SPAC27F1.04c.1:pep; SPAC27F1.04c.
GeneIDi2542004.
KEGGispo:SPAC27F1.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA93293.1.
PIRiT38462.
RefSeqiNP_594532.1. NM_001019961.2.

3D structure databases

ProteinModelPortaliQ10173.
SMRiQ10173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278487. 17 interactors.
DIPiDIP-36501N.
IntActiQ10173. 7 interactors.
MINTiMINT-4697479.

PTM databases

iPTMnetiQ10173.

Proteomic databases

MaxQBiQ10173.
PRIDEiQ10173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC27F1.04c.1; SPAC27F1.04c.1:pep; SPAC27F1.04c.
GeneIDi2542004.
KEGGispo:SPAC27F1.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC27F1.04c.
PomBaseiSPAC27F1.04c. nuf2.

Phylogenomic databases

InParanoidiQ10173.
KOiK11548.
OMAiQIFEWFA.
OrthoDBiEOG092C2OAH.
PhylomeDBiQ10173.

Miscellaneous databases

PROiQ10173.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUF2_SCHPO
AccessioniPrimary (citable) accession number: Q10173
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.