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Protein

Adenine DNA glycosylase

Gene

myh1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adenine glycosylase active on G-A mispairs. Has glycosylase and nicking activities and is active at A/G and A/GO sites.1 Publication

Catalytic activityi

Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69Proton donor/acceptorBy similarity1
Sitei172Transition state stabilizerBy similarity1
Metal bindingi226Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi233Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi236Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi242Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity Source: PomBase
  • adenine/guanine mispair binding Source: PomBase
  • metal ion binding Source: UniProtKB-KW
  • oxidized purine DNA binding Source: PomBase
  • purine-specific mismatch base pair DNA N-glycosylase activity Source: PomBase

GO - Biological processi

  • base-excision repair Source: PomBase
  • base-excision repair, AP site formation Source: PomBase
  • cellular response to DNA damage stimulus Source: PomBase
  • cellular response to UV Source: PomBase
  • depurination Source: PomBase
  • detection of DNA damage stimulus involved in DNA damage checkpoint Source: PomBase
  • mismatch repair Source: PomBase
  • positive regulation of phosphodiesterase I activity Source: PomBase

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-110331 Cleavage of the damaged purine

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine DNA glycosylase (EC:3.2.2.31)
Gene namesi
Name:myh1
Synonyms:myh
ORF Names:SPAC26A3.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC26A3.02
PomBaseiSPAC26A3.02 myh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022421 – 461Adenine DNA glycosylaseAdd BLAST461

Proteomic databases

MaxQBiQ10159
PaxDbiQ10159
PRIDEiQ10159

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi279177, 18 interactors
IntActiQ10159, 4 interactors
STRINGi4896.SPAC26A3.02.1

Structurei

3D structure databases

ProteinModelPortaliQ10159
SMRiQ10159
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini296 – 437Nudix hydrolaseAdd BLAST142

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi340 – 366Nudix boxAdd BLAST27

Sequence similaritiesi

Belongs to the Nth/MutY family.Curated

Phylogenomic databases

InParanoidiQ10159
KOiK03575
OMAiISRFYAI
OrthoDBiEOG092C1L64
PhylomeDBiQ10159

Family and domain databases

CDDicd03431 DNA_Glycosylase_C, 1 hit
cd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR029119 MutY_C
IPR015797 NUDIX_hydrolase-like_dom_sf
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
PF14815 NUDIX_4, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q10159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSNHSLDL HSYTQLEVER FRESLIQFYD KTKRILPWRK KECIPPSEDS
60 70 80 90 100
PLEDWEQPVQ RLYEVLVSEI MLQQTRVETV KRYYTKWMET LPTLKSCAEA
110 120 130 140 150
EYNTQVMPLW SGMGFYTRCK RLHQACQHLA KLHPSEIPRT GDEWAKGIPG
160 170 180 190 200
VGPYTAGAVL SIAWKQPTGI VDGNVIRVLS RALAIHSDCS KGKANALIWK
210 220 230 240 250
LANELVDPVR PGDFNQALME LGAITCTPQS PRCSVCPISE ICKAYQEQNV
260 270 280 290 300
IRDGNTIKYD IEDVPCNICI TDIPSKEDLQ NWVVARYPVH PAKTKQREER
310 320 330 340 350
ALVVIFQKTD PSTKEKFFLI RKRPSAGLLA GLWDFPTIEF GQESWPKDMD
360 370 380 390 400
AEFQKSIAQW ISNDSRSLIK KYQSRGRYLH IFSHIRKTSH VFYAIASPDI
410 420 430 440 450
VTNEDFFWIS QSDLEHVGMC ELGLKNYRAA LEIKKRKVTS LSNFKEPKLT
460
SARRIVTKAE C
Length:461
Mass (Da):52,931
Last modified:October 1, 1996 - v1
Checksum:i5908E880202E2242
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → F in AAC36207 (PubMed:9737967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053340 mRNA Translation: AAC36207.1
CU329670 Genomic DNA Translation: CAA93225.1
PIRiT38390
T43679
RefSeqiNP_594145.1, NM_001019569.2

Genome annotation databases

EnsemblFungiiSPAC26A3.02.1; SPAC26A3.02.1:pep; SPAC26A3.02
GeneIDi2542727
KEGGispo:SPAC26A3.02

Similar proteinsi

Entry informationi

Entry nameiMYH1_SCHPO
AccessioniPrimary (citable) accession number: Q10159
Secondary accession number(s): O74679
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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