Q10155 (RNZ1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease Z 1
Short name=RNase Z 1
EC=3.1.26.11

Alternative name(s):
tRNA 3 endonuclease 1
tRNase Z 1

Gene names

Name:	trz1
ORF Names:	SPAC1D4.10

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. May be involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Ref.3
Catalytic activity	Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.
Cofactor	Zinc Probable.
Subcellular location	Nucleus. Cytoplasm Ref.2 Ref.3.
Sequence similarities	Belongs to the RNase Z family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA 3'-trailer cleavage Inferred from mutant phenotype PubMed 19555350. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.2. Source: PomBase nucleus Inferred from direct assay Ref.2Ref.3. Source: PomBase
Molecular_function	3'-tRNA processing endoribonuclease activity Inferred from genetic interaction PubMed 19555350. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Ribonuclease Z 1		PRO_0000155836

Sequences

	Sequence		Length	Mass (Da)
	Q10155 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 350FBE7B05FBF880	FASTA	809	90,603
	10 20 30 40 50 60 MSKTVNFRAT KNFYLQFVSV SSRDTSCIPC IHLFFDSKRY VFGSVGEGCQ RAILSQQLRL 70 80 90 100 110 120 SKIKDVFLMQ GSSISSPDTY DSSSSSSTTS VSDMLQLDDR DKVIVSERNS MCSTVNYPTW 130 140 150 160 170 180 WDSCGGFPGF LLSLNDISEP GETGEASPFV LHGPSEVHQF LSSMRHFTYH TNVNLTVQGY 190 200 210 220 230 240 TSAEAPVFVD ENICVTPVVV SLVKNSFKKR KHENINRGTN ARPLKEDRAN TSPHWYSHVS 250 260 270 280 290 300 NDTSFVVENA MYNTPAPLEP DKPELFISYI VQSHPTPGKF DAAKAKSLGI TKGLDCGRLA 310 320 330 340 350 360 RGEPVTLENG KTVYPKEVIG PSIPGSSFFI IHCPNELVID LVIENHKWTN APKPVCVIHS 370 380 390 400 410 420 VTPEVYKNPR YQSWISSFPS EVSHLIASTE VNEVINYPRS AVAIATLNLL DSKVFPLGFN 430 440 450 460 470 480 CYEVKNVQKN NRIAFAKPKL RFAFGKKTGI DDSEVGVSIE ELKDKILKEK PDYKSFVEEA 490 500 510 520 530 540 QKYVSDKPKA PSFAGSDIQI CTLGTGSAMP SLYRNVSSTY VRIPVDKKCM EDSAISMKNI 550 560 570 580 590 600 LLDCGEGTLG RLSRQYGDNL KYEIASLRWI YISHMHADHH AGVIGVLKAW TKYSDGRSKL 610 620 630 640 650 660 FITAPPQFEF WLLEYSRIDY LPLSNIVFIS NSALRTDRKP SALESSRLSS LFKEFDLVSF 670 680 690 700 710 720 RTVPAIHCPY SYCMEITNSS GWKIAYSGDT RPSEDFANIA KDSTLLIHEA TLEDSMHEIA 730 740 750 760 770 780 IKKQHSTYSE ALEVAKKAGT KNVILTHFSQ RYPKLPDIDI STEDLHIALA FDGMTLKISD 790 800 ISLFRYFGKP LAYLFNEENL KEESDPLKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"The fission yeast Schizosaccharomyces pombe has two distinct tRNase Z(L)s encoded by two different genes and differentially targeted to the nucleus and mitochondria." Gan X., Yang J., Li J., Yu H., Dai H., Liu J., Huang Y. Biochem. J. 435:103-111(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA93219.1.
PIR	T38051.
RefSeq	NP_593023.1. NM_001018422.2.
3D structure databases
ProteinModelPortal	Q10155.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC1D4.10-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC1D4.10.1; SPAC1D4.10.1:pep; SPAC1D4.10.
GeneID	2542457.
KEGG	spo:SPAC1D4.10.
Organism-specific databases
PomBase	SPAC1D4.10.
Phylogenomic databases
eggNOG	COG1234.
KO	K00784.
OMA	HENINRG.
OrthoDB	EOG4JQ75P.
Family and domain databases
InterPro	IPR001279. Beta-lactamas-like. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20803513.

Entry information

Entry name

RNZ1_SCHPO

Accession

Primary (citable) accession number: Q10155

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program