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Q10119 (EF1A2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 1-alpha-B/C
Short name=EF-1-alpha-B/C
Gene names
Name:tef1b
Synonyms:ef1a-b
ORF Names:SPAC23A1.10
AND
Name:tef1c
Synonyms:ef1a-c, tef1d
ORF Names:SPBC24E9.15c, SPBC839.15c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Elongation factor 1-alpha-B/C
PRO_0000090969

Regions

Nucleotide binding14 – 218GTP By similarity
Nucleotide binding91 – 955GTP By similarity
Nucleotide binding153 – 1564GTP By similarity

Amino acid modifications

Modified residue231Phosphothreonine Ref.6
Modified residue241Phosphothreonine Ref.6
Modified residue1581Phosphothreonine Ref.6
Modified residue2891Phosphoserine Ref.6
Modified residue2961Phosphoserine Ref.6
Modified residue3141Phosphoserine Ref.6
Modified residue3881Phosphoserine Ref.6
Modified residue3941Phosphoserine Ref.6

Experimental info

Sequence conflict211S → F in BAA11571. Ref.1
Sequence conflict1391L → R in BAA11571. Ref.1
Sequence conflict1391L → V in BAA19867. Ref.2
Sequence conflict3881S → A in AAP86554. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q10119 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2D8429568A5F98D4

FASTA46049,675
        10         20         30         40         50         60 
MGKEKGHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEATELG KGSFKYAWVL 

        70         80         90        100        110        120 
DKLKAERERG ITIDIALWKF ETPKYNVTVI DAPGHRDFIK NMITGTSQAD CAILIIGGGT 

       130        140        150        160        170        180 
GEFEAGISKD GQTREHALLA YTLGVKQLIV AVNKMDTTGW SQARFEEIVK ETSNFIKKVG 

       190        200        210        220        230        240 
FNPKTVPFVP VSGFQGDNMI EPTTNMPWYQ GWQKETKAGV VKGKTLLEAI DSIEPPARPT 

       250        260        270        280        290        300 
DKPLRLPLQD VYKIGGIGTV PVGRVETGVI KPGMIVTFAP AGVTTEVKSV EMHHESLDAG 

       310        320        330        340        350        360 
LPGDNVGFNV KNVSVKDIRR GNVCGDSKND PPMGCASFTA QVIILNHPGQ ISAGYSPVLD 

       370        380        390        400        410        420 
CHTAHIACKF AELIEKIDRR SGKKIEESPK FVKSGDACIA KMVPSKPMCV EAFTDYAPLG 

       430        440        450        460 
RFAVRDMRQT VAVGVIKAVE KVAPGAAKVT KAAVKAGAKK

« Hide

References

« Hide 'large scale' references
[1]"Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors."
Mita K., Morimyo M., Ito K., Sugaya K., Ebihara K., Hongo E., Higashi T., Hirayama Y., Nakamura Y.
Gene 187:259-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (TEF1B AND TEF1C).
Strain: 972 / ATCC 24843.
[2]"Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: PR745.
[3]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (TEF1B AND TEF1C).
Strain: 972 / ATCC 24843.
[4]"Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses."
Kurtzman C.P., Robnett C.J.
FEMS Yeast Res. 3:417-432(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-397.
Strain: NRRL Y-12796.
[5]"Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1alpha and 1beta(gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombe."
Miyazaki M., Uritani M., Fujimura K., Yamakatsu H., Kageyama T., Takahashi K.
J. Biochem. 103:508-521(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 64-94.
[6]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23; THR-24; THR-158; SER-289; SER-296; SER-314; SER-388 AND SER-394, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D82572 mRNA. Translation: BAA11570.1.
D82573 mRNA. Translation: BAA11571.1.
D89112 mRNA. Translation: BAA19867.1.
CU329670 Genomic DNA. Translation: CAA16984.1.
CU329671 Genomic DNA. Translation: CAB46708.1.
AF402093 Genomic DNA. Translation: AAP86554.1.
PIRB41453.
T38230.
T42089.
RefSeqNP_594440.1. NM_001019869.2.
NP_595255.1. NM_001021161.2.

3D structure databases

ProteinModelPortalQ10119.
SMRQ10119. Positions 2-441.
ModBaseSearch...

Protein-protein interaction databases

IntActQ10119. 1 interaction.
STRING4896.SPBC839.15c-1.

Proteomic databases

PRIDEQ10119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC23A1.10.1; SPAC23A1.10.1:pep; SPAC23A1.10.
SPBC839.15c.1; SPBC839.15c.1:pep; SPBC839.15c.
GeneID2539917.
2541984.
KEGGspo:SPAC23A1.10.
spo:SPBC839.15c.

Organism-specific databases

PomBaseSPAC23A1.10.
SPBC839.15c.

Phylogenomic databases

HOGENOMHOG000229291.
KOK03231.
OMALEPSANC.
OrthoDBEOG4BS0V0.

Family and domain databases

InterProIPR000795. EF_GTP-bd_dom.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50465. Elong_init_C. 1 hit.
SSF50447. Translat_factor. 1 hit.
TIGRFAMsTIGR00483. EF-1_alpha. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801060.

Entry information

Entry nameEF1A2_SCHPO
AccessionPrimary (citable) accession number: Q10119
Secondary accession number(s): P78764 expand/collapse secondary AC list , Q10158, Q7M4U9, Q7Z8V5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents