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Q10119

- EF1A2_SCHPO

UniProt

Q10119 - EF1A2_SCHPO

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Protein
Elongation factor 1-alpha-B/C
Gene
tef1b, ef1a-b, SPAC23A1.10
tef1c, ef1a-c, tef1d, SPBC24E9.15c, SPBC839.15c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTP By similarity
Nucleotide bindingi91 – 955GTP By similarity
Nucleotide bindingi153 – 1564GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: InterPro
  3. translation elongation factor activity Source: PomBase
Complete GO annotation...

GO - Biological processi

  1. cytoplasmic translational elongation Source: PomBase
  2. translational elongation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_202295. HSF1 activation.

Names & Taxonomyi

Organism-specific databases

Protein namesi
Recommended name:
Elongation factor 1-alpha-B/C
Short name:
EF-1-alpha-B/C
Gene namesi
Name:tef1b
Synonyms:ef1a-b
ORF Names:SPAC23A1.10
AND
Name:tef1c
Synonyms:ef1a-c, tef1d
ORF Names:SPBC24E9.15c, SPBC839.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I, UP000002485: Chromosome II
PomBaseiSPAC23A1.10.
SPBC839.15c.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. eukaryotic translation elongation factor 1 complex Source: PomBase
  3. nucleus Source: PomBase

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Elongation factor 1-alpha-B/CUniRule annotation
PRO_0000090969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphothreonine1 Publication
Modified residuei24 – 241Phosphothreonine1 Publication
Modified residuei158 – 1581Phosphothreonine1 Publication
Modified residuei289 – 2891Phosphoserine1 Publication
Modified residuei296 – 2961Phosphoserine1 Publication
Modified residuei314 – 3141Phosphoserine1 Publication
Modified residuei388 – 3881Phosphoserine1 Publication
Modified residuei394 – 3941Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ10119.
PRIDEiQ10119.

Interactioni

Protein-protein interaction databases

BioGridi276461. 6 interactions.
278469. 6 interactions.
IntActiQ10119. 1 interaction.
MINTiMINT-4697049.
STRINGi4896.SPBC839.15c-1.

Structurei

3D structure databases

ProteinModelPortaliQ10119.
SMRiQ10119. Positions 2-441.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 240236tr-type G
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1 By similarity
Regioni70 – 745G2 By similarity
Regioni91 – 944G3 By similarity
Regioni153 – 1564G4 By similarity
Regioni192 – 1943G5 By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000229291.
KOiK03231.
OMAiVWHPSAI.
OrthoDBiEOG715QCW.
PhylomeDBiQ10119.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10119-1 [UniParc]FASTAAdd to Basket

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MGKEKGHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEATELG    50
KGSFKYAWVL DKLKAERERG ITIDIALWKF ETPKYNVTVI DAPGHRDFIK 100
NMITGTSQAD CAILIIGGGT GEFEAGISKD GQTREHALLA YTLGVKQLIV 150
AVNKMDTTGW SQARFEEIVK ETSNFIKKVG FNPKTVPFVP VSGFQGDNMI 200
EPTTNMPWYQ GWQKETKAGV VKGKTLLEAI DSIEPPARPT DKPLRLPLQD 250
VYKIGGIGTV PVGRVETGVI KPGMIVTFAP AGVTTEVKSV EMHHESLDAG 300
LPGDNVGFNV KNVSVKDIRR GNVCGDSKND PPMGCASFTA QVIILNHPGQ 350
ISAGYSPVLD CHTAHIACKF AELIEKIDRR SGKKIEESPK FVKSGDACIA 400
KMVPSKPMCV EAFTDYAPLG RFAVRDMRQT VAVGVIKAVE KVAPGAAKVT 450
KAAVKAGAKK 460
Length:460
Mass (Da):49,675
Last modified:November 1, 1996 - v1
Checksum:i2D8429568A5F98D4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211S → F in BAA11571. 1 Publication
Sequence conflicti139 – 1391L → R in BAA11571. 1 Publication
Sequence conflicti139 – 1391L → V in BAA19867. 1 Publication
Sequence conflicti388 – 3881S → A in AAP86554. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82572 mRNA. Translation: BAA11570.1.
D82573 mRNA. Translation: BAA11571.1.
D89112 mRNA. Translation: BAA19867.1.
CU329670 Genomic DNA. Translation: CAA16984.1.
CU329671 Genomic DNA. Translation: CAB46708.1.
AF402093 Genomic DNA. Translation: AAP86554.1.
PIRiB41453.
T38230.
T42089.
RefSeqiNP_594440.1. NM_001019869.2.
NP_595255.1. NM_001021161.2.

Genome annotation databases

EnsemblFungiiSPAC23A1.10.1; SPAC23A1.10.1:pep; SPAC23A1.10.
SPBC839.15c.1; SPBC839.15c.1:pep; SPBC839.15c.
GeneIDi2539917.
2541984.
KEGGispo:SPAC23A1.10.
spo:SPBC839.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82572 mRNA. Translation: BAA11570.1 .
D82573 mRNA. Translation: BAA11571.1 .
D89112 mRNA. Translation: BAA19867.1 .
CU329670 Genomic DNA. Translation: CAA16984.1 .
CU329671 Genomic DNA. Translation: CAB46708.1 .
AF402093 Genomic DNA. Translation: AAP86554.1 .
PIRi B41453.
T38230.
T42089.
RefSeqi NP_594440.1. NM_001019869.2.
NP_595255.1. NM_001021161.2.

3D structure databases

ProteinModelPortali Q10119.
SMRi Q10119. Positions 2-441.
ModBasei Search...

Protein-protein interaction databases

BioGridi 276461. 6 interactions.
278469. 6 interactions.
IntActi Q10119. 1 interaction.
MINTi MINT-4697049.
STRINGi 4896.SPBC839.15c-1.

Proteomic databases

MaxQBi Q10119.
PRIDEi Q10119.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC23A1.10.1 ; SPAC23A1.10.1:pep ; SPAC23A1.10 .
SPBC839.15c.1 ; SPBC839.15c.1:pep ; SPBC839.15c .
GeneIDi 2539917.
2541984.
KEGGi spo:SPAC23A1.10.
spo:SPBC839.15c.

Organism-specific databases

PomBasei SPAC23A1.10.
SPBC839.15c.

Phylogenomic databases

HOGENOMi HOG000229291.
KOi K03231.
OMAi VWHPSAI.
OrthoDBi EOG715QCW.
PhylomeDBi Q10119.

Enzyme and pathway databases

Reactomei REACT_202295. HSF1 activation.

Miscellaneous databases

NextBioi 20801060.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00118_A. EF_Tu_A.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00483. EF-1_alpha. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors."
    Mita K., Morimyo M., Ito K., Sugaya K., Ebihara K., Hongo E., Higashi T., Hirayama Y., Nakamura Y.
    Gene 187:259-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (TEF1B AND TEF1C).
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: PR745.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (TEF1B AND TEF1C).
    Strain: 972 / ATCC 24843.
  4. "Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses."
    Kurtzman C.P., Robnett C.J.
    FEMS Yeast Res. 3:417-432(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-397.
    Strain: NRRL Y-12796.
  5. "Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1alpha and 1beta(gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombe."
    Miyazaki M., Uritani M., Fujimura K., Yamakatsu H., Kageyama T., Takahashi K.
    J. Biochem. 103:508-521(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 64-94.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23; THR-24; THR-158; SER-289; SER-296; SER-314; SER-388 AND SER-394, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEF1A2_SCHPO
AccessioniPrimary (citable) accession number: Q10119
Secondary accession number(s): P78764
, Q10158, Q7M4U9, Q7Z8V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi