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Protein

Glutathione gamma-glutamylcysteinyltransferase

Gene

SPAC3H1.10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for detoxification of heavy metals such as cadmium and arsenate.2 Publications

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

GO - Molecular functioni

  • cadmium ion binding Source: PomBase
  • glutathione gamma-glutamylcysteinyltransferase activity Source: PomBase

GO - Biological processi

  • cellular response to cadmium ion Source: PomBase
  • detoxification of arsenic-containing substance Source: PomBase
  • detoxification of cadmium ion Source: PomBase
  • detoxification of copper ion Source: PomBase
  • phytochelatin biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Cadmium, Copper, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase (EC:2.3.2.15)
Alternative name(s):
Phytochelatin synthase
Gene namesi
ORF Names:SPAC3H1.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3H1.10.
PomBaseiSPAC3H1.10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Glutathione gamma-glutamylcysteinyltransferasePRO_0000116449Add
BLAST

Proteomic databases

MaxQBiQ10075.

Expressioni

Inductioni

By cadmium, copper and zinc.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi279789. 4 interactions.
MINTiMINT-4696717.
STRINGi4896.SPAC3H1.10-1.

Structurei

3D structure databases

ProteinModelPortaliQ10075.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 256220Peptidase C83PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG76926.
HOGENOMiHOG000209121.
InParanoidiQ10075.
KOiK05941.
OrthoDBiEOG7GFBFP.
PhylomeDBiQ10075.

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIVKRAVPE LLRGMTNATP NIGLIKNKVV SFEAVGQLKK SFYKRQLPKQ
60 70 80 90 100
CLAFDSSLGK DVFLRALQEG RMENYFSLAQ QMVTQNEPAF CGLGTLCMIL
110 120 130 140 150
NSLKVDPGRL WKGSWRWYDQ YMLDCCRSLS DIEKDGVTLE EFSCLANCNG
160 170 180 190 200
LRTITKCVKD VSFDEFRKDV ISCSTIENKI MAISFCRKVL GQTGDGHFSP
210 220 230 240 250
VGGFSESDNK ILILDVARFK YPCYWVDLKL MYESMFPIDK ASGQPRGYVL
260 270 280 290 300
LEPMHIPLGV LTVGLNKYSW RNVSKHILQQ AATVKNADNL AEILLSINQS
310 320 330 340 350
SIPLIQERSN SSKSGDFEHF KECIRSTKTY HLFLKHTNTN VEYITMAFWA
360 370 380 390 400
IFSLPMIQKA LPKGVLEEIQ SLLKEVEISE INTQLTALKK QLDSLTHCCK
410
TDTGCCSSSC CKNT
Length:414
Mass (Da):46,741
Last modified:February 1, 1996 - v1
Checksum:i4EAB522E9C322EBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA92263.1.
PIRiT38742.
RefSeqiNP_593552.1. NM_001018985.2.

Genome annotation databases

EnsemblFungiiSPAC3H1.10.1; SPAC3H1.10.1:pep; SPAC3H1.10.
GeneIDi2543367.
KEGGispo:SPAC3H1.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA92263.1.
PIRiT38742.
RefSeqiNP_593552.1. NM_001018985.2.

3D structure databases

ProteinModelPortaliQ10075.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279789. 4 interactions.
MINTiMINT-4696717.
STRINGi4896.SPAC3H1.10-1.

Proteomic databases

MaxQBiQ10075.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3H1.10.1; SPAC3H1.10.1:pep; SPAC3H1.10.
GeneIDi2543367.
KEGGispo:SPAC3H1.10.

Organism-specific databases

EuPathDBiFungiDB:SPAC3H1.10.
PomBaseiSPAC3H1.10.

Phylogenomic databases

eggNOGiNOG76926.
HOGENOMiHOG000209121.
InParanoidiQ10075.
KOiK05941.
OrthoDBiEOG7GFBFP.
PhylomeDBiQ10075.

Miscellaneous databases

NextBioi20804382.
PROiQ10075.

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe."
    Ha S.-B., Smith A.P., Howden R., Dietrich W.M., Bugg S., O'Connell M.J., Goldsbrough P.B., Cobbett C.S.
    Plant Cell 11:1153-1164(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  3. "Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast."
    Clemens S., Kim E.J., Neumann D., Schroeder J.I.
    EMBO J. 18:3325-3333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPCS_SCHPO
AccessioniPrimary (citable) accession number: Q10075
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.