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Protein

Arginase

Gene

aru1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase (aru1), Arginase (car1)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Manganese 1PROSITE-ProRule annotation
Metal bindingi142 – 1421Manganese 1PROSITE-ProRule annotation
Metal bindingi142 – 1421Manganese 2PROSITE-ProRule annotation
Metal bindingi144 – 1441Manganese 2PROSITE-ProRule annotation
Metal bindingi146 – 1461Manganese 1PROSITE-ProRule annotation
Binding sitei198 – 1981SubstrateBy similarity
Metal bindingi247 – 2471Manganese 1PROSITE-ProRule annotation
Metal bindingi247 – 2471Manganese 2PROSITE-ProRule annotation
Metal bindingi249 – 2491Manganese 2PROSITE-ProRule annotation
Binding sitei261 – 2611SubstrateBy similarity
Binding sitei292 – 2921SubstrateBy similarity

GO - Molecular functioni

  • arginase activity Source: PomBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • arginine catabolic process to ornithine Source: PomBase
  • arginine catabolic process to proline via ornithine Source: PomBase
  • urea cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism, Urea cycle

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-70635. Urea cycle.
UniPathwayiUPA00158; UER00270.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase (EC:3.5.3.1By similarity)
Gene namesi
Name:aru1
ORF Names:SPAC3H1.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3H1.07.
PomBaseiSPAC3H1.07. aru1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323ArginasePRO_0000173710Add
BLAST

Proteomic databases

MaxQBiQ10066.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi279799. 17 interactions.
MINTiMINT-4696665.

Structurei

3D structure databases

ProteinModelPortaliQ10066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1485Substrate bindingBy similarity
Regioni155 – 1573Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000204319.
InParanoidiQ10066.
KOiK01476.
OMAiSSEHANM.
OrthoDBiEOG7X0VT2.
PhylomeDBiQ10066.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiIPR014033. Arginase.
IPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
[Graphical view]
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiPF00491. Arginase. 1 hit.
[Graphical view]
PRINTSiPR00116. ARGINASE.
TIGRFAMsiTIGR01229. rocF_arginase. 1 hit.
PROSITEiPS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPHKISDNH RHLMLSRFMM GNGVSIINMP FSGGQPKDGA ELAPEMVEKA
60 70 80 90 100
GLVDDLEHLG YDVKLIQNPE FKSRPSKEGP NQALMKNPLY VSNVTRQVRD
110 120 130 140 150
AVQRELEQQR VVVNIGGDHS LAIGTVEGVQ AVYDDACVLW IDAHADINTP
160 170 180 190 200
ESSPSKNLHG CPLSFSLGYA EPLPEEFAWT KRVIEERRLA FIGLRDLDPM
210 220 230 240 250
ERAFLRERNI AAYTMHHVDK YGIGRVVEMA MEHINPGKRR PVHLSFDVDA
260 270 280 290 300
CDPIVAPATG TRVPGGLTFR EAMYICEAVA ESGTLVAVDV MEVNPLLGNE
310 320
EEAKTTVDLA RSIVRTSLGQ TLL
Length:323
Mass (Da):35,704
Last modified:February 1, 1996 - v1
Checksum:i1E10876E0E969D66
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti278 – 2781A → R in AAA65456 (Ref. 1) Curated
Sequence conflicti293 – 2942Missing in AAA65456 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24279 Genomic DNA. Translation: AAA65456.1.
CU329670 Genomic DNA. Translation: CAA92260.1.
PIRiT38739.
T52537.
RefSeqiNP_593549.1. NM_001018982.2.

Genome annotation databases

EnsemblFungiiSPAC3H1.07.1; SPAC3H1.07.1:pep; SPAC3H1.07.
GeneIDi2543377.
KEGGispo:SPAC3H1.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24279 Genomic DNA. Translation: AAA65456.1.
CU329670 Genomic DNA. Translation: CAA92260.1.
PIRiT38739.
T52537.
RefSeqiNP_593549.1. NM_001018982.2.

3D structure databases

ProteinModelPortaliQ10066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279799. 17 interactions.
MINTiMINT-4696665.

Proteomic databases

MaxQBiQ10066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3H1.07.1; SPAC3H1.07.1:pep; SPAC3H1.07.
GeneIDi2543377.
KEGGispo:SPAC3H1.07.

Organism-specific databases

EuPathDBiFungiDB:SPAC3H1.07.
PomBaseiSPAC3H1.07. aru1.

Phylogenomic databases

HOGENOMiHOG000204319.
InParanoidiQ10066.
KOiK01476.
OMAiSSEHANM.
OrthoDBiEOG7X0VT2.
PhylomeDBiQ10066.

Enzyme and pathway databases

UniPathwayiUPA00158; UER00270.
ReactomeiR-SPO-70635. Urea cycle.

Miscellaneous databases

PROiQ10066.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiIPR014033. Arginase.
IPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
[Graphical view]
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiPF00491. Arginase. 1 hit.
[Graphical view]
PRINTSiPR00116. ARGINASE.
TIGRFAMsiTIGR01229. rocF_arginase. 1 hit.
PROSITEiPS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Ocampos M.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiARGI2_SCHPO
AccessioniPrimary (citable) accession number: Q10066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.