Reviewed, UniProtKB/Swiss-Prot Q10062 (PPOX_SCHPO)

Names and origin

Protein names

Recommended name:
    Protoporphyrinogen oxidase
      Short name=PPO
    EC=1.3.3.4

Gene names

Name:	hem14
ORF Names:	SPAC1F5.07c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	FAD By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protoporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Protoporphyrinogen oxidase		PRO_0000116445
Regions
	Nucleotide binding	7 – 12	6	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10062-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CB679F6A437547AA	FASTA	490	53,462
	10 20 30 40 50 60 MSIAICGGGI AGLSTAFYLA RLIPKCTIDL YEKGPRLGGW LQSVKIPCAD SPTGTVLFEQ 70 80 90 100 110 120 GPRTLRPAGV AGLANLDLIS KLGIEDKLLR ISSNSPSAKN RYIYYPDRLN EIPSSILGSI 130 140 150 160 170 180 KSIMQPALRP MPLAMMLEPF RKSKRDSTDE SVGSFMRRRF GKNVTDRVMS AMINGIYAGD 190 200 210 220 230 240 LNDLSMHSSM FGFLAKIEKK YGNITLGLIR ALLAREILSP AEKALKAALL AEPKTAELSN 250 260 270 280 290 300 SMKSTSMFAF KEGIETITLS IADELKKMPN VKIHLNKPAK TLVPHKTQSL VDVNGQAYEY 310 320 330 340 350 360 VVFANSSRNL ENLISCPKME TPTSSVYVVN VYYKDPNVLP IRGFGLLIPS CTPNNPNHVL 370 380 390 400 410 420 GIVFDSEQNN PENGSKVTVM MGGSAYTKNT SLIPTNPEEA VNNALKALQH TLKISSKPTL 430 440 450 460 470 480 TNATLQQNCI PQYRVGHQDN LNSLKSWIEK NMGGRILLTG SWYNGVSIGD CIMNGHSTAR 490 KLASLMNSSS

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAA92235.1.
PIR	T38088.
RefSeq	NP_592866.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2541631.
KEGG	spo:SPAC1F5.07c.
NMPDR	fig|4896.1.peg.2836.
Organism-specific databases
GeneDB_Spombe	SPAC1F5.07c.
Phylogenomic databases
OMA	Q10062. ETGISYI.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000601-MON.
BRENDA	1.3.3.4. 653.
Gene expression databases
ArrayExpress	Q10062.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR004572. Protoporphyrinogen_oxidase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX_SCHPO

Accession

Primary (citable) accession number: Q10062

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)