Q10045 (UNC50_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein unc-50

Alternative name(s):
Uncoordinated protein 50

Gene names

Name:	unc-50
ORF Names:	T07A5.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for cell surface expression of acetylcholine receptors in body-wall muscles. Ref.3
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein Ref.3.
Developmental stage	Ubiquitously expressed, from early embryogenesis to adulthood. Ref.3
Sequence similarities	Belongs to the unc-50 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to Golgi membrane Inferred from direct assay Ref.3. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Protein unc-50		PRO_0000065452
Regions
	Topological domain	1 – 115	115	Cytoplasmic Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Topological domain	137 – 142	6	Lumenal Potential
	Transmembrane	143 – 163	21	Helical; Potential
	Topological domain	164 – 193	30	Cytoplasmic Potential
	Transmembrane	194 – 214	21	Helical; Potential
	Topological domain	215 – 221	7	Lumenal Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Topological domain	243 – 254	12	Cytoplasmic Potential
	Transmembrane	255 – 275	21	Helical; Potential
	Topological domain	276 – 301	26	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q10045 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 186A5B6AE302DDEA	FASTA	301	34,739
	10 20 30 40 50 60 MSSQPRGSGT QPGPSQSPIS QRNFRYEPAR SGYTSPGQYS TYSTSTADRV GCLTAVRMSA 70 80 90 100 110 120 FAKLSRFTRR LVHIRQMDFE FALWQMLYLL IQPSKVYKNF IYRKRTKDQF ARDDPAFLVL 130 140 150 160 170 180 LALSLLFSSI FYAYALGLEK IGFFTFFLWS VFVDCIGVGV VIATVLWWVS NRFLRKVRDQ 190 200 210 220 230 240 DVEWGYCFDV HLNAFFPMLI LLHVIVPILY PTLIDSPAFL SILLGNTFWF LAACYYVYIT 250 260 270 280 290 300 FLGYTALPIL HKTQYFLYPI SFIFMFFVAT LTGGWNISRT ALNFYHSRAE PHKFAPQHGG L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein." Fitzgerald J., Kennedy D., Viseshakul N., Cohen B.N., Mattick J., Bateman J.F., Forsayeth J.R. Brain Res. 877:110-123(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[3]	"Regulation of nicotinic receptor trafficking by the transmembrane Golgi protein UNC-50." Eimer S., Gottschalk A., Hengartner M., Horvitz H.R., Richmond J., Schafer W.R., Bessereau J.-L. EMBO J. 26:4313-4323(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48055 Genomic DNA. Translation: CAA88132.2.
PIR	T24631.
RefSeq	NP_499279.2. NM_066878.3.
UniGene	Cel.22852.
3D structure databases
ProteinModelPortal	Q10045.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.T07A5.2.
Proteomic databases
PaxDb	Q10045.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T07A5.2; T07A5.2; T07A5.2.
GeneID	176443.
KEGG	cel:CELE_T07A5.2.
UCSC	T07A5.2. c. elegans.
Organism-specific databases
CTD	176443.
WormBase	T07A5.2; CE36501; WBGene00006785; unc-50.
Phylogenomic databases
eggNOG	NOG262379.
GeneTree	ENSGT00390000018553.
HOGENOM	HOG000193563.
InParanoid	Q10045.
OMA	HLNAFFP.
Family and domain databases
InterPro	IPR007881. UNC-50. [Graphical view]
PANTHER	PTHR12841. PTHR12841. 1 hit.
Pfam	PF05216. UNC-50. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	892606.

Entry information

Entry name

UNC50_CAEEL

Accession

Primary (citable) accession number: Q10045

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 4, 2005
Last modified:	May 29, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project