Tyrosine-protein phosphatase vhp-1 - Caenorhabditis elegans

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Q10038 (VHP1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase vhp-1
EC=3.1.3.48

Alternative name(s):
Vh1 dual specificity phosphatase family protein 1

Gene names

Name:	vhp-1
ORF Names:	F08B1.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts preferentially on the c-Jun N-terminal kinase (JNK) and p38 MAPKs. Plays an important role in the heavy metal stress response by negatively regulating the kgb-1 (JNK-like) and the pmk-1 (p38-type) MAPK signaling pathways. Ref.1
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate.
Tissue specificity	Expressed in the pharynx, intestine, neurons and vulval hypodermal cells. Ref.1
Developmental stage	Expressed throughout development. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of JNK cascade Inferred from direct assay Ref.1. Source: WormBase nematode larval development Inferred from mutant phenotype Ref.1. Source: WormBase peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC response to copper ion Inferred from genetic interaction Ref.1. Source: WormBase
Cellular_component	cytoplasm Inferred from sequence or structural similarity Ref.1. Source: WormBase nucleus Inferred from sequence or structural similarity Ref.1. Source: WormBase
Molecular_function	JUN kinase phosphatase activity Inferred from direct assay Ref.1. Source: WormBase MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from direct assay Ref.1. Source: WormBase protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q10038-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q10038-2) The sequence of this isoform differs from the canonical sequence as follows: 1-136: MTVLDTVTIS...DKFRSVMVLE → MGGQPFEAFR...SPISSSSPTN
Note: No experimental confirmation available.

Isoform c (identifier: Q10038-3) The sequence of this isoform differs from the canonical sequence as follows: 1-155: Missing. 588-657: ASSTPGTSRA...TSSLEIPCHQ → VSIMKLH
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 657

657

Tyrosine-protein phosphatase vhp-1

PRO_0000094925

Regions

Domain

21 – 151

131

Rhodanese

Domain

175 – 317

143

Tyrosine-protein phosphatase

Compositional bias

350 – 565

216

Ser-rich

Sites

Active site

262

Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence

1 – 155

155

Missing in isoform c.

VSP_014013

Alternative sequence

1 – 136

136

MTVLD…VMVLE → MGGQPFEAFRKNRKRKKTKN KKKRRNNNNSKNKTPNTFPN EIEEQDPVSSLPTFPAKKFG LKLQLTLTSSPTNSSSPISS SSPTN in isoform b.

VSP_014014

Alternative sequence

588 – 657

ASSTP…IPCHQ → VSIMKLH in isoform c.

VSP_014015

Sequences

Sequence		Length	Mass (Da)
Isoform a [UniParc]. Last modified June 7, 2005. Version 2. Checksum: A0D9153DE6326B43 Show »	FASTA	657	71,003
10 20 30 40 50 60 MTVLDTVTIS TCGLAALIRE APDTTLVVDC RGFTEYNESH VRHSMNAFFS KLIRRRLFEN 70 80 90 100 110 120 KLDDNCLIHQ LMSCSSGCTK MDEKLDLVLY AEEDKPRGNK RRIASCNAPE STAKIMRVLR 130 140 150 160 170 180 ERLEDTDKFR SVMVLEGGFK QFAQQYPQLC ESSEGMTRLP QSLSQPCLSQ PTGDGITLIT 190 200 210 220 230 240 PNIYLGSQID SLDETMLDAL DISVVINLSM TCPKSVCIKE DKNFMRIPVN DSYQEKLSPY 250 260 270 280 290 300 FPMAYEFLEK CRRAGKKCLI HCLAGISRSP TLAISYIMRY MKMGSDDAYR YVKERRPSIS 310 320 330 340 350 360 PNFNFMGQLL EYENVLIKDH VLDYNQASRP HRHMDYYGPS DLCPPKVPKS ASSNCVFPGS 370 380 390 400 410 420 THDESSPSSP SVSEGSAASE PETSSSAASS SSTASAPPSM PSTSEQGTSS GTVNVNGKRN 430 440 450 460 470 480 MTMDLGLPHR PKALGLPSRI GTSVAELPSP STELSRLSFN GPEAIAPSTP ILNFTNPCFN 490 500 510 520 530 540 SPIIPVASSS REVILTLPTP AASSSSSTSS EPSFDFSSFE SSSSSSIVVE NPFFASTEVP 550 560 570 580 590 600 AGSSSISTPS GSQSTPASAS SSAASRCRMK GFFKVFSKKA PASTSTPASS TPGTSRAARP 610 620 630 640 650 ECLRSSGIII SAPVLAITEE EDAESPESGF NEPEVGEEDD DSVSICSTSS LEIPCHQ « Hide
Isoform b [UniParc]. Checksum: 5B71795C6ED58058 Show »	FASTA	606	64,963
10 20 30 40 50 60 MGGQPFEAFR KNRKRKKTKN KKKRRNNNNS KNKTPNTFPN EIEEQDPVSS LPTFPAKKFG 70 80 90 100 110 120 LKLQLTLTSS PTNSSSPISS SSPTNGGFKQ FAQQYPQLCE SSEGMTRLPQ SLSQPCLSQP 130 140 150 160 170 180 TGDGITLITP NIYLGSQIDS LDETMLDALD ISVVINLSMT CPKSVCIKED KNFMRIPVND 190 200 210 220 230 240 SYQEKLSPYF PMAYEFLEKC RRAGKKCLIH CLAGISRSPT LAISYIMRYM KMGSDDAYRY 250 260 270 280 290 300 VKERRPSISP NFNFMGQLLE YENVLIKDHV LDYNQASRPH RHMDYYGPSD LCPPKVPKSA 310 320 330 340 350 360 SSNCVFPGST HDESSPSSPS VSEGSAASEP ETSSSAASSS STASAPPSMP STSEQGTSSG 370 380 390 400 410 420 TVNVNGKRNM TMDLGLPHRP KALGLPSRIG TSVAELPSPS TELSRLSFNG PEAIAPSTPI 430 440 450 460 470 480 LNFTNPCFNS PIIPVASSSR EVILTLPTPA ASSSSSTSSE PSFDFSSFES SSSSSIVVEN 490 500 510 520 530 540 PFFASTEVPA GSSSISTPSG SQSTPASASS SAASRCRMKG FFKVFSKKAP ASTSTPASST 550 560 570 580 590 600 PGTSRAARPE CLRSSGIIIS APVLAITEEE DAESPESGFN EPEVGEEDDD SVSICSTSSL EIPCHQ « Hide
Isoform c [UniParc]. Checksum: A7FF64BDC46B0774 Show »	FASTA	439	46,893
10 20 30 40 50 60 MTRLPQSLSQ PCLSQPTGDG ITLITPNIYL GSQIDSLDET MLDALDISVV INLSMTCPKS 70 80 90 100 110 120 VCIKEDKNFM RIPVNDSYQE KLSPYFPMAY EFLEKCRRAG KKCLIHCLAG ISRSPTLAIS 130 140 150 160 170 180 YIMRYMKMGS DDAYRYVKER RPSISPNFNF MGQLLEYENV LIKDHVLDYN QASRPHRHMD 190 200 210 220 230 240 YYGPSDLCPP KVPKSASSNC VFPGSTHDES SPSSPSVSEG SAASEPETSS SAASSSSTAS 250 260 270 280 290 300 APPSMPSTSE QGTSSGTVNV NGKRNMTMDL GLPHRPKALG LPSRIGTSVA ELPSPSTELS 310 320 330 340 350 360 RLSFNGPEAI APSTPILNFT NPCFNSPIIP VASSSREVIL TLPTPAASSS SSTSSEPSFD 370 380 390 400 410 420 FSSFESSSSS SIVVENPFFA STEVPAGSSS ISTPSGSQST PASASSSAAS RCRMKGFFKV 430 FSKKAPASTS TPVSIMKLH « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Caenorhabditis elegans MAPK phosphatase VHP-1 mediates a novel JNK-like signaling pathway in stress response." Mizuno T., Hisamoto N., Terada T., Kondo T., Adachi M., Nishida E., Kim D.H., Ausubel F.M., Matsumoto K. EMBO J. 23:2226-2234(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY585194 mRNA. Translation: AAS91377.1. FO081082 Genomic DNA. Translation: CCD68973.1. FO081082 Genomic DNA. Translation: CCD68974.1. FO081082 Genomic DNA. Translation: CCD68975.1.
PIR	T15969.
RefSeq	NP_494997.1. NM_062596.4. NP_494998.1. NM_062597.1. NP_871926.1. NM_182126.3.
UniGene	Cel.8018.
3D structure databases
ProteinModelPortal	Q10038.
SMR	Q10038. Positions 9-319.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59690N.
Proteomic databases
PaxDb	Q10038.
PRIDE	Q10038.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F08B1.1a.1; F08B1.1a.1; F08B1.1. F08B1.1a.2; F08B1.1a.2; F08B1.1. F08B1.1a.3; F08B1.1a.3; F08B1.1. F08B1.1a.4; F08B1.1a.4; F08B1.1.
GeneID	173904.
KEGG	cel:CELE_F08B1.1.
UCSC	F08B1.1a.3. c. elegans.
Organism-specific databases
CTD	173904.
WormBase	F08B1.1a; CE27918; WBGene00006923; vhp-1. F08B1.1b; CE27919; WBGene00006923; vhp-1. F08B1.1c; CE32357; WBGene00006923; vhp-1.
Phylogenomic databases
eggNOG	COG2453.
GeneTree	ENSGT00700000104093.
HOGENOM	HOG000017063.
InParanoid	Q10038.
KO	K04459.
OMA	KRRIASC.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR008343. MKP. IPR001763. Rhodanese-like_dom. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	881603.

Entry information

Entry name

VHP1_CAEEL

Accession

Primary (citable) accession number: Q10038
Secondary accession number(s): Q8IG35, Q8ST18, Q8ST19

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents